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Secondary Metabolites of Miconia rubiginosa
Juliana Rodrigues,Daniel Rinaldo,Marcelo Aparecido da Silva,Lourdes Campaner dos Santos,Wagner Vilegas 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.7
The Miconia genus is the most representative of the Melastomataceae family, and some species are commonly used in Brazilian folk medicine as anti-inflammatory agents. In this work we investigated the leaves from Miconia rubiginosa (Bonpl.) DC, using high-speed countercurrent chromatography, which yielded 11 substances (eight flavonoids, gallic acid, casuarictin, and schizandriside). Identification was achieved using nuclear magnetic resonance spectroscopy and high-performance liquid chromatography–circular dichroism–diode array detection analyses.
Antimicrobial Activity of Miconia Species (Melastomataceae)
Juliana Rodrigues,Danielle Carvalho Michelin,Daniel Rinaldo,Guilherme Juli? Zocolo,Lourdes Campaner dos Santos,Wagner Vilegas,H?ida Regina Nunes Salgado 한국식품영양과학회 2008 Journal of medicinal food Vol.11 No.1
This work evaluated the antimicrobial activity of the methanol and chloroform extracts of the leaves of Mi-conia cabucu, Miconia rubiginosa, and Miconia stenostachya using the disc-diffusion method. The results obtained showedthat the methanol extracts of the leaves of M. rubiginosaand M. stenostachyaand the chloroform extract of the leaves of M.cabucu presented antimicrobial activity against the tested microorganisms.
Daryne L.M.G. Costa,Daniel Rinaldo,Eliana A. Varanda,Juliana F. de Sousa,Ana L.M. Nasser,Ana C.Z. Silva,Debora C. Baldoqui,Wagner Vilegas,Lourdes Campaner dos Santos 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.10
It is well known that phytotherapy has grown in popularity in recent years. Because a drug cannot be administered without ensuring its effectiveness and safety, the standardization and regulation of phytotherapeutic drugs are required by the global market and governmental authorities. This article describes a simple and reliable high-performance liquid chromatography–diode array detection analysis method for the simultaneous detection of myricetin-3-O-b-Dgalactopyranoside, myricetin-3-O-a-L-arabinopyranoside, and myricetin-3-O-a-L-rhaminopyranoside present in the hydroethanolic extract (ethanol/H2O, 7:3, v/v) of Pouteria torta. The mutagenic activity of the extract was evaluated on Salmonella typhimurium and by an in vivo micronucleus test on the peripheral blood cells of Swiss mice. The linearity, sensitivity, selectivity, repeatability, accuracy, and precision of the assay were evaluated. The analytical curves were linear and exhibited good repeatability (with a deviation of less than 5%) and demonstrated good recovery (within the 83–107% range). The results demonstrate that the hydroethanolic extract exhibited a mutagenic activity in both assays, suggesting caution in the use of this plant in folk medicine.