Curdione Inhibits Proliferation of MCF-7 Cells by Inducing Apoptosis

Li, Juan,Bian, Wei-He,Wan, Juan,Zhou, Jing,Lin, Yan,Wang, Ji-Rong,Wang, Zhao-Xia,Shen, Qun,Wang, Ke-Ming Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Background: Curdione, one of the major components of Curcuma zedoaria, has been reported to possess various biological activities. It thus might be a candidate anti-flammatory and cancer chemopreventive agent. However, the precise molecular mechanisms of action of curdione on cancer cells are still unclear. In this study, we investigated the effect of curdione on breast cancer. Materials and Methods: Xenograft nude mice were used to detect the effect of curdione on breast cancer in vivo; we also tested the effect of curdione on breast cancer in vitro by MTT, Flow cytometry, JC-I assay, and western blot. Results: Firstly, we found that curdione significantly suppressed tumor growth in a xenograft nude mouse breast tumor model in a dose-dependent manner. In addition, curdione treatment inhibited cell proliferation and induced cell apoptosis. Moreover, after curdione treatment, increase of impaired mitochondrial membrane potential occurred in a concentration dependent manner. Furthermore, the expression of apoptosis-related proteins including cleaved caspase-3, caspase-9 and Bax was increased in curdione treatment groups, while the expression of the anti-apoptotic Bcl-2 was decreased. Inhibitors of caspase-3 were used to confirm that curdione induced apoptosis. Conclusions: Overall, our observations first suggested that curdione inhibited the proliferation of breast cancer cells by inducing apoptosis. These results might provide some molecular basis for the anti-cancer activity of curdione.

Mitochondrial DNA Levels in Blood and Tissue Samples from Breast Cancer Patients of Different Stages

Xia, Peng,Wang, Hui-Juan,Geng, Ting-Ting,Xun, Xiao-Jie,Zhou, Wen-Jing,Jin, Tian-Bo,Chen, Chao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.3

Aims: Alterations in mitochondrial DNA (mtDNA) have been implicated in carcinogenesis and tumor progression. We here evaluated the diagnostic and prognostic potential of mtDNA as a biomarker for breast cancer. Methods: Using multiplex real-time polymerase chain reaction, nuclear DNA (nDNA) and mtDNA levels in serum, buffy coat, tumor, and tumor-adjacent tissue samples from 50 breast cancer patients were determined and assessed for associations with clinicopathological features. To evaluate mtDNA as a biomarker for distinguishing between the four sample types, we created receiver operating characteristic (ROC) curves. Results: The mtDNA levels in buffy coat were significantly lower than in other sample types. Relative to tumor-adjacent tissue, reduced levels of mtDNA were identified in buffy coat and tumor tissue but not in serum. According to ROC curve analysis, mtDNA levels could be used to distinguish between buffy coat and tumor-adjacent tissue samples with good sensitivity (77%) and specificity (83%). Moreover, mtDNA levels in serum and tumor tissue were positively associated with cancer TMN stage. Conclusions: The mtDNA levels in blood samples may represent a promising, non-invasive biomarker in breast cancer patients. Additional, large-scale validation studies are required to establish the potential use of mtDNA levels in the early diagnosis and monitoring of breast cancer.

Antifungal activity of 3-acetylbenzamide produced by actinomycete WA23-4-4 from the intestinal tract of Periplaneta americana

Xia Fang,Juan Shen,Jie Wang,Zhi-li Chen,Pei-bin lin,Zhi-yu Chen,Lin-yan Liu,Huan-xiong Zeng,Xiao-bao Jin 한국미생물학회 2018 The journal of microbiology Vol.56 No.7

Actinomycetes are well-known for producing numerous bioactive secondary metabolites. In this study, primary screening by antifungal activity assay found one actinomycete strain WA23-4-4 isolated from the intestinal tract of Periplaneta americana that exhibited broad spectrum antifungal activity. 16S rDNA gene analysis of strain WA23-4-4 revealed close similarity to Streptomyces nogalater (AB045886) with 86.6% sequence similarity. Strain WA23-4-4 was considered as a novel Streptomyces and the 16s rDNA sequence has been submitted to GenBank (accession no. KX291006). The maximum antifungal activity of WA23-4-4 was achieved when culture conditions were optimized to pH 8.0, with 12% inoculum concentration and 210 ml ISP2 medium, which remained stable between the 5th and the 9th day. 3-Acetyl benzoyl amide was isolated by ethyl acetate extraction of WA23- 4-4 fermentation broth, and its molecular formula was determined as C9H9NO2 based on MS, IR, 1H, and 13C NMR analyses. The compound showed significant antifungal activity against Candida albicans ATCC 10231 (MIC: 31.25 μg/ml) and Aspergillus niger ATCC 16404 (MIC: 31.25 μg/ml). However, the compound had higher MIC values against Trichophyton rubrum ATCC 60836 (MIC: 500 μg/ml) and Aspergillus fumigatus ATCC 96918 (MIC: 1,000 μg/ml). SEM analysis showed damage to the cell membrane of Candida albicans ATCC 10231 and to the mycelium of Aspergillus niger ATCC 16404 after being treatment with 3-acetyl benzoyl amide. In conclusion, this is the first time that 3-acetyl benzoyl amide has been identified from an actinomycete and this compound exhibited antifungal activity against Candida albicans ATCC 10231 and Aspergillus niger ATCC 16404.

Prestressed concrete bridges with corrugated steel webs: Nonlinear analysis and experimental investigation

Xia-chun Chen,Zhi-zhou Bai,Yu Zeng,Rui-juan Jiang,Francis T.K. Au 국제구조공학회 2016 Steel and Composite Structures, An International J Vol.21 No.5

Concrete bridges with corrugated steel webs and prestressed by both internal and external tendons have emerged as one of the promising bridge forms. In view of the different behaviour of components and the large shear deformation of webs with negligible flexural stiffness, the assumption that plane sections remain plane may no longer be valid, and therefore the classical Euler-Bernoulli and Timoshenko beam models may not be applicable. In the design of this type of bridges, both the ultimate load and ductility should be examined, which requires the estimation of full-range behaviour. An analytical sandwich beam model and its corresponding beam finite element model for geometric and material nonlinear analysis are developed for this type of bridges considering the diaphragm effects. Different rotations are assigned to the flanges and corrugated steel webs to describe the displacements. The model accounts for the interaction between the axial and flexural deformations of the beam, and uses the actual stress-strain curves of materials considering their stress path-dependence. With a nonlinear kinematical theory, complete description of the nonlinear interaction between the external tendons and the beam is obtained. The numerical model proposed is verified by experiments.

Interactions Between MTHFR C677T - A1298C Variants and Folic Acid Deficiency Affect Breast Cancer Risk in a Chinese Population

Wu, Xia-Yu,Ni, Juan,Xu, Wei-Jiang,Zhou, Tao,Wang, Xu Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Background: Our objective was to evaluate the MTHFR C677T-A1298C polymorphisms in patients with breast cancer and in individuals with no history of cancer, to compare the levels of genetic damage and apoptosis under folic acid (FA) deficiency between patients and controls, and to assess associations with breast cancer. Methods: Genetic damage was marked by micronucleated binucleated cells (MNBN) and apoptosis was estimated by cytokinesis-block micronucleus assay (CBMN). PCR-RFLP molecular analysis was carried out. Results: The results showed significant associations between the MTHFR 677TT or the combined MTHFR C677T-A1298C and breast cancer risk (OR = 2.51, CI = 0.85 to 7.37, p = 0.08; OR = 4.11, CI = 0.78 to 21.8, p < 0.001). The MNBN from the combined MTHFR C677T-A1298C was higher and the apoptosis was lower than that of the single variants (p < 0.05). At 15 to 60 nmol/L FA, the MNBN in cases with the TTAC genotype was higher than controls (p < 0.05), whereas no significant difference in apoptosis was found between the cases and controls after excluding the genetic background. Conclusions: Associations between the combined MTHFR C677T-A1298C polymorphism and breast cancer are possible from this study. A dose of 120 nmol/L FA could enhance apoptosis in cases with MTHFR C677T-A1298C. Breast cancer individuals with the TTAC genotype may be more sensitive to the genotoxic effects of FA deficiency than controls.

Genetic Variant in CLPTM1L Confers Reduced Risk of Lung Cancer: a Replication Study in Chinese and a Meta-analysis

Luo, Xia,Lamsal, Laxmi Pangeni,Xu, Wen-Juan,Lu, Jie,Lu, Yan-Jun,Shen, Ying,Guan, Qing Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

Background: Rs31489 in the cleft lip and palate transmembrane1-like gene (CLPTM1L) has been identified to be associated with lung cancer through genome-wide association studies (GWAS). However, some recent replication studies yielded inconclusive results. Thus, we undertook this study to investigate the precise effect of rs31489 on lung cancer susceptibility. Materials and Methods: A hospital-based case-control study in 1,673 Chinese subjects (611 individuals with lung cancer and 1,062 controls) and a meta-analysis among 32,199 subjects (16,364 cases and 15,835 controls) were performed in this study. Results: In our case-control study, rs31489 was inversely associated with lung cancer (AC versus CC: OR=0.68, 95%CI=0.52-0.88; additive model: OR=0.68, 95%CI=0.54-0.85; dominant model: OR=0.65, 95%CI =0.51-0.84). Stratification analysis by smoking status showed a significant association and strong genetic effect in non-smokers but not in smokers. Our meta-analysis further confirmed the association, although with significant heterogeneity contributed by study design and source of controls, as shown by stratified analysis. Sensitive and cumulative analyses both indicated robust stability of our results. In addition, there was no observable publication bias in our meta-analysis. Conclusions: Overall, the findings from our replication study and meta-analysis demonstrated that CLPTM1L gene rs31489 is significantly associated with lung cancer.

Energy efficiency characteristic analysis of tri‑coil PT symmetric MC‑WPT systems

Zhi-Juan Liao,Qi-Wei Zhu,Wen Ren,Chen-Yang Xia,Xu Liu 전력전자학회 2023 JOURNAL OF POWER ELECTRONICS Vol.23 No.9

The parity-time (PT) symmetric magnetic coupling wireless power transfer (MC-WPT) system has received a great deal of attention since it was proposed. Its transmission efficiency has been greatly improved when compared with previous research. The operational amplifier (OA) is a typical construction method for PT symmetric MC-WPT systems. On this basis, to achieve a higher transmission efficiency and a longer effective power transmission distance at the same time, this paper constructs an OA-based tri-coil PT symmetric MC-WPT system. The analytical expressions of its singularity, PT symmetric state, and PT symmetric broken state are obtained. Then a complete set of parameter design criteria for the tri-coil PT symmetric system is derived. The transmission efficiency and resonant frequency of two-coil and tri-coil system are simulated on MATLAB software, and the simulation results are consistent with the theoretical analysis results. Finally, an experimental device is constructed to further verify the correctness of the theory. This paper demonstrates that the effective power transmission distance of the tri-coil PT symmetric MC-WPT system is more than twice that of the two-coil PT symmetric MC-WPT system, which can achieve a good balance between transmission efficiency and transmission distance.

An Iterative Algorithm for Solving the Least-Squares Problem of Matrix Equation AXB+CYD=E

Kai-juan Shen,Chuan-hua You,Yu-xia Du 한국전산응용수학회 2008 Journal of applied mathematics & informatics Vol.26 No.5

In this paper, an iterative method is proposed to solve the least-squares problem of matrix equation AXB + CY D = E over un-known matrix pair [X, Y ]. By this iterative method, for any initial matrix pair [X1, Y1], a solution pair or the least-norm least-squares solution pair of which can be obtained within finite iterative steps in the absence of roundoff errors. In addition, we also consider the optimal approximation problem for the given matrix pair [X0, Y0] in Frobenius norm. Given numerical examples show that the algorithm is efficient. In this paper, an iterative method is proposed to solve the least-squares problem of matrix equation AXB + CY D = E over un-known matrix pair [X, Y ]. By this iterative method, for any initial matrix pair [X1, Y1], a solution pair or the least-norm least-squares solution pair of which can be obtained within finite iterative steps in the absence of roundoff errors. In addition, we also consider the optimal approximation problem for the given matrix pair [X0, Y0] in Frobenius norm. Given numerical examples show that the algorithm is efficient.

Realgar bioleaching solution suppress ras excessive activation by increasing ROS in Caenorhabditis elegans

De Juan Zhi,Na Feng,Dong Ling Liu,Rong Li Hou,Mei Zu Wang,Xiao Xia Ding,Hong Yu Li 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.3

Although realgar bioleaching solution (RBS) hasbeen proved to be a potential candidate for cancer therapy, themechanisms of RBS anticancer are still far from beingcompletely understood. Dosed with RBS in C. elegans, themultivulva phenotype resulting from oncogenic ras gain-offunctionwasinhibited in a dose dependentmanner. It could beabrogated by concurrent treatment C. elegans with RBS andthe radical scavengerDMSO. However, RBS could not induceDAF-16 nuclear translocation in TJ356 or the increase of HSP16.2 expression in CL2070, which both could be arousedvisible GFP fluorescent variation to represent for oxidativestress generation. Treatment C. elegans with superoxide aniongenerator paraquat, similar results were also obtained. Ourresults indicated that RBS suppress excessive activated ras byincreasing reactive oxygen species (ROS) in C. elegans. Secondly, ROS induced by RBS significantly accumulated ona higher level inC. elegans with amutational ras than that withwild ras, thus leading to oxidative stress on ras gain-of-functionbackground rather than on normal ras context. Our resultsfirstly demonstrated that using C. elegans as amodel organismfor evaluating prooxidant drug candidates for cancer therapy.

HIST1H2BN induced cell proliferation and EMT phenotype in prostate cancer via NF-κB signal pathway

Zhang Juan,Chang Yuhan,Xia Haiyan,Xu Luwei,Wei Xiaowei 한국유전학회 2021 Genes & Genomics Vol.43 No.11

Background The potential role of HIST1H2BN in prostate cancer remains unclear. Objective To evaluate the carcinogenic role of HIST1H2BN in prostate cancer. Methods The expression of HIST1H2BN in prostate cancer was analyzed using TCGA database and clinical samples. The roles and mechanisms of HIST1H2BN were investigated in DU145 and PC3 cells. Results HIST1H2BN was signifcantly upregulated in prostate cancer. HIST1H2BN knockdown inhibited cell proliferation, migration and EMT phenotype in prostate cancer cells. Downregulating HIST1H2BN diminished the expression and binding activity of NF-κB p65, then infuenced the expression of MMP2 and MMP9. Conclusion This is the frst study to elaborate a HIST1H2BN-NF-κB-EMT regulatory axis in oncogenesis, indicating that HIST1H2BN might be potential therapeutic target for prostate cancer.