Effects of Aqueous Extract of Schizandra Chinensis Fruit on Cadmium-Induced Change of Monoamine Neurotransmitters in Rats

Zhao, Zheng Lin,Zhao, Guang Wen,Li, Li,Li, Meng Quan,Guan, Li Xin,Yang, Xu Dong,Li, Hou Zhong,Lin, Feng,Lee, Jong-Rok,Zhao, Rong Jie Korean Society of ToxicologyKorea Environmental Mu 2009 Toxicological Research Vol.26 No.1

The effects of aqueous extract of Schizandra Chinensis Fruit (AESC) on cadmium-induced changes of monoamine neurotransmitters in the different brain regions of adult rats were investigated. Male rats were received intraperitoneal (i.p.) administration of CdCl2 (0.6 mg/kg/d) for 21 days and sacrificed 7 days after the last administration. Concentrations of norepinephrine (NE), dopamine (DA) in striatum and serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) in cortex were measured by HPLC. There were significant decreases of NE, DA, 5-HT and 5-HIAA in Cd intoxicated rats (P < 0.05), while pretreatment with AESC (20 mg/kg/d or 60 mg/kg/d, p.o., 30 min before $CdCl_2$) greatly inhibited the decrease of monoamine transmitters, respectively (P < 0.05). Also, AESC significantly increased the reduction of glutathione contents and superoxide dismutase activities in cortex induced by $CdCl_2$. These results suggest that AESC ameliorates Cd-induced depletion of monoamine neurotransmitters in brain through its antioxidant activity.

Effects of Aqueous Extract of Schizandra Chinensis Fruit on Cadmium-Induced Change of Monoamine Neurotransmitters in Rats

Zheng Lin Zhao,Guang Wen Zhao,Li Li,Meng Quan Li,Li Xin Guan,Xu Dong Yang,Hou Zhong Li,Feng Lin,Jong Rok Lee,Rong Jie Zhao 한국독성학회 2009 Toxicological Research Vol.25 No.1

The effects of aqueous extract of Schizandra Chinensis Fruit (AESC) on cadmium-induced changes of monoamine neurotransmitters in the different brain regions of adult rats were investigated. Male rats were received intraperitoneal (i.p.) administration of CdCl₂ (0.6 ㎎/㎏/d) for 21 days and sacrificed 7 days after the last administration. Concentrations of norepinephrine (NE), dopamine (DA) in striatum and serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) in cortex were measured by HPLC. There were significant decreases of NE, DA, 5-HT and 5-HIAA in Cd intoxicated rats (P < 0.05), while pretreatment with AESC (20 ㎎/㎏/d or 60 mg/kg/d, p.o., 30 min before CdCl₂) greatly inhibited the decrease of monoamine transmitters, respectively (P < 0.05). Also, AESC significantly increased the reduction of glutathione contents and superoxide dismutase activities in cortex induced by CdCl₂. These results suggest that AESC ameliorates Cd-induced depletion of monoamine neurotransmitters in brain through its antioxidant activity.

Hypoxia Induced High Expression of Thioredoxin Interacting Protein (TXNIP) in Non-small Cell Lung Cancer and its Prognostic Effect

Li, Yan,Miao, Li-Yun,Xiao, Yong-Long,Huang, Mei,Yu, Min,Meng, Kui,Cai, Hou-Rong Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.7

Although associations between thioredoxin interacting protein (TXNIP) and cancers have been recognized, the effects of TXNIP on non-small cell lung cancer (NSCLC) prognosis remained to be determined in detail. In addition, while hypoxia is a key characteristic of tumor cell growth microenvironment, the effect of hypoxia on TXNIP expression is controversial. In this study, formaldehyde fixed and paraffin embedded (FFPE) samples of 70 NSCLC patients who underwent resection between January 2010 and December 2011 were obtained. Evaluation of TXNIP and hypoxia inducible factor-$1{\alpha}$ ($HIF-1{\alpha}$) protein expression in FFPE samples was made by immunohistochemistry. By Kaplan-Meier method, patients with high TXNIP expression demonstrated a significantly shorter progression free survival (PFS) compared with those with low TXNIP expression (18.0 months, 95%CI: 11.7, 24.3 versus 23.0 months, 95%CI: 17.6, 28.4, P=0.02). High TXNIP expression level was also identified as an independent prognostic factor by Cox regression analysis (adjusted hazard ratio: 2.46; 95%CI: 1.08, 5.56; P=0.03). Furthermore, TXNIP expression was found to be significantly correlated with $HIF-1{\alpha}$ expression (Spearman correlation=0.67, P=0.000). To further confirm correlations, we established a tumor cell hypoxic culture model. Expression of TXNIP was up-regulated in all three NSCLC cell lines (A549, SPC-A1, and H1299) under hypoxic conditions. This study suggests that hypoxia induces increased TXNIP expression in NSCLC and high TXNIP expression could be a poor prognostic marker.

Elevated Platelets Enhance Cancer Cell Migration, Promote Hematogenous Metastasis and Associate with a Poor Prognosis in Advanced Non-small Cell Lung Cancer Cases

Li, Yan,Miao, Li-Yun,Xiao, Yong-Long,Cai, Hou-Rong,Zhang, De-Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

Although correlations between platelets and lung cancer has been recognized, effects on non-small cell lung cancer (NSCLC) metastasis remain to be determined in detail. In the present study, wound healing assays revealed a role of platelets in NSCLC cell migration. Thus the mean migration rate of lung adenocarcinoma A549 cells was significantly elevated after co-culture with platelets ($81.7{\pm}0.45%$ vs $41.0{\pm}3.50%$, P<0.01). Expression of GAPDH was examined by reverse transcription-polymerase chain reaction to study the effect of platelets on NSCLC cell proliferation. The result showed that the proliferation of A549 and SPC-A1 cells was not affected. Mouse models were established by transfusing A549 cells and SPC-A1 cells into mice lateral tail veins. We found tumor metastasis nodules in lungs to be increased significantly after co-transfusion with platelets (in A549, $4.33{\pm}0.33$ vs $0.33{\pm}0.33$, P=0.01; in SPC-A1, $2.67{\pm}0.33$ vs $0.00{\pm}0.00$, P=0.01). In addition, consecutive inoperable patients with newly diagnosed NSCLC (TNM stage III or IV) between January 2009 and December 2011 were retrospectively reviewed. Using the Kaplan-Meier method, NSCLC patients with a high platelet counts demonstrated a significantly shorter progression free survival compared with those with a low platelet count (> $200{\times}10^9/L$, 3 months versus ${\leq}200{\times}10^9/L$, 5 months, P=0.001). An elevated platelet count was also identified as an independent prognostic factor by Cox regression analysis for prgression free survival (adjusted hazard ratio: 1.69; 95% CI: 1.16, 2.46; P=0.006). This study suggested that platelets might contribute to the hematogenous metastatic process by promoting cancer cell migration, which eventually affects the prognosis of NSCLC.

Distinct Transcriptional and Functional Differences of Lung Resident and Monocyte-Derived Alveolar Macrophages During the Recovery Period of Acute Lung Injury

Hou Fei,Wang Huan,Zheng Kun,Yang Wenting,Xiao Kun,Rong Zihan,Xiao Junjie,Li Jing,Cheng Baihe,Tang Li,Xie Lixin 대한면역학회 2023 Immune Network Vol.23 No.3

In acute lung injury, two subsets of lung macrophages exist in the alveoli: tissue-resident alveolar macrophages (AMs) and monocyte-derived alveolar macrophages (MDMs). However, it is unclear whether these 2 subsets of macrophages have different functions and characteristics during the recovery phase. RNA-sequencing of AMs and MDMs from the recovery period of LPS-induced lung injury mice revealed their differences in proliferation, cell death, phagocytosis, inflammation and tissue repair. Using flow cytometry, we found that AMs showed a higher ability to proliferate, whereas MDMs expressed a larger amount of cell death. We also compared the ability of phagocytosing apoptotic cells and activating adaptive immunity and found that AMs have a stronger ability to phagocytose, while MDMs are the cells that activate lymphocytes during the resolving phase. By testing surface markers, we found that MDMs were more prone to the M1 phenotype, but expressed a higher level of pro-repairing genes. Finally, analysis of a publicly available set of single-cell RNA-sequencing data on bronchoalveolar lavage cells from patients with SARS-CoV-2 infection validated the double-sided role of MDMs. Blockade of inflammatory MDM recruitment using CCR2−/− mice effectively attenuates lung injury. Therefore, AMs and MDMs exhibited large differences during recovery. AMs are long-lived M2-like tissue-resident macrophages that have a strong ability to proliferate and phagocytose. MDMs are a paradoxical group of macrophages that promote the repair of tissue damage despite being strongly pro-inflammatory early in infection, and they may undergo cell death as inflammation fades. Preventing the massive recruitment of inflammatory MDMs or promoting their transition to pro-repairing phenotype may be a new direction for the treatment of acute lung injury.

Convolutional neural network-based data anomaly detection considering class imbalance with limited data

Lingfang Li,Yao Du,Rong-rong Hou,Xiaoyou Wang,Wei Tian,Yong Xia 국제구조공학회 2022 Smart Structures and Systems, An International Jou Vol.29 No.1

The raw data collected by structural health monitoring (SHM) systems may suffer multiple patterns of anomalies, which pose a significant barrier for an automatic and accurate structural condition assessment. Therefore, the detection and classification of these anomalies is an essential pre-processing step for SHM systems. However, the heterogeneous data patterns scarce anomalous samples and severe class imbalance make data anomaly detection difficult. In this regard, this study proposes a convolutional neural network-based data anomaly detection method. The time and frequency domains data are transferred as images and used as the input of the neural network for training. ResNet18 is adopted as the feature extractor to avoid training with massive labelled data. In addition, the focal loss function is adopted to soften the class imbalance-induced classification bias. The effectiveness of the proposed method is validated using acceleration data collected in a long-span cable-stayed bridge. The proposed approach detects and classifies data anomalies with high accuracy.

Unsupervised one-class classification for condition assessment of bridge cables using Bayesian factor analysis

Lingfang Li,Xiaoyou Wang,Wei Tian,Yao Du,Rong-rong Hou,Yong Xia 국제구조공학회 2022 Smart Structures and Systems, An International Jou Vol.29 No.1

Cables are critical components of cable-stayed bridges. A structural health monitoring system provides real-time cable tension recording for cable health monitoring. However, the measurement data involve multiple sources of variability, i.e., varying environmental and operational factors, which increase the complexity of cable condition monitoring. In this study, a one-class classification method is developed for cable condition assessment using Bayesian factor analysis (FA). The singlepeaked vehicle-induced cable tension is assumed to be relevant to vehicle positions and weights. The Bayesian FA is adopted to establish the correlation model between cable tensions and vehicles. Vehicle weights are assumed to be latent variables and the influences of different transverse positions are quantified by coefficient parameters. The Bayesian theorem is employed to estimate the parameters and variables automatically, and the damage index is defined on the basis of the well-trained model. The proposed method is applied to one cable-stayed bridge for cable damage detection. Significant deviations of the damage indices of Cable SJS11 were observed, indicating a damaged condition in 2011. This study develops a novel method to evaluate the health condition of individual cable using the FA in the Bayesian framework. Only vehicle-induced cable tensions are used and there is no need to monitor the vehicles. The entire process, including the data pre-processing, model training and damage index calculation of one cable, takes only 35 s, which is highly efficient.

Diversity of Duodenal and Rectal Microbiota in Biopsy Tissues and Luminal Contents in Healthy Volunteers(s)

( Gangping Li ),( Min Yang ),( Kan Zhou ),( Lei Zhang ),( Lugao Tian ),( Shangze Lv ),( Yu Jin ),( Wei Qian ),( Hanhua Xiong ),( Rong Lin ),( Yu Fu ),( Xiaohua Hou ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.7

The diverse microbial communities that colonize distinct segments of the gastrointestinal tract are intimately related to aspects of physiology and the pathology of human health. However, most recent studies have focused on the rectal or fecal microbiota, and the microbial signature of the duodenum is poorly studied. In this study, we compared the microbiota in duodenal and rectal samples to illustrate the characteristic microbial signatures of the duodenum in healthy adults. Nine healthy volunteers donated biopsies and luminal contents from the duodenum and rectum. To determine the composition and diversity of the microbiota, 454- pyrosequencing of bacterial 16S rRNA was performed and multiple bioinformatics analyses were applied. The α-diversity and phylogenetic diversity of the microbiota in the duodenal samples were higher than those of the rectal samples. There was higher biodiversity among the microbiota isolated from rectal biopsies than feces. Proteobacteria were more highly represented in the duodenum than in the rectum, both in the biopsies and in the luminal contents from the healthy volunteers (38.7% versus 12.5%, 33.2% versus 5.0%, respectively). Acinetobacter and Prevotella were dominant in the duodenum, whereas Bacteroides and Prevotella were dominant in the rectum. Additionally, the percentage of OTUs shared in biopsy groups was far higher than in the luminal group (43.0% versus 26.8%) and a greater number of genera was shared among the biopsies than the luminal contents. Duodenal samples demonstrated greater biological diversity and possessed a unique microbial signature compared with the rectum. The mucosa-associated microbiota was more relatively conserved than luminal samples.

論文 : 488例淸代溫病醫案的舌象分析

양영 ( Rong Liang ),이연 ( Ya Li ),왕성화 ( Sheng Hua Wang ),후양방 ( Yang Fang Hou ),관군달 ( Jun Da Guan ) 대한한의학원전학회 ( 구 대한원전의사학회 ) 2006 대한한의학원전학회지 Vol.19 No.3

Expression of Tumor Necrosis Factor Receptor-associated Factor 6 in Lung Cancer Tissues

Zhang, Xiu-Ling,Dang, Yi-Wu,Li, Ping,Rong, Min-Hua,Hou, Xin-Xi,Luo, Dian-Zhong,Chen, Gang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Background: Tumor necrosis factor (TNF) receptor-associated factor 6 (TRAF6) has been reported to be associated with the development of various cancers. However, the role of TRAF6 in lung cancer remains unclear. Objective: To explore the expression and clinicopathological significance of TRAF6 protein in lung cancer tissues. Materials and Methods: Three hundred and sixty-five lung cancer samples and thirty normal lung tissues were constructed into 3 microarrays. The expression of TRAF6 protein was determined using immunohistochemistry (IHC). Furthermore, correlations between the expression of TRAF6 and clinicopathological parameters were investigated. Results: The expression of TRAF6 in total lung cancer tissues (365 cases), as well as in small cell lung cancer (SCLC, 26 cases) and non-small cell lung cancer (NSCLC, 339 cases) was significantly higher compared with that in normal lung tissues. The ROC curve showed that the area under curve of TRAF6 was 0.663 (95%CI 0.570~0.756) for lung cancer. The diagnostic sensitivity and specificity of TRAF6 were 52.6% and 80%, respectively. In addition, the expression of TRAF6 was correlated with clinical TNM stage, tumor size and lymph node metastasis in all lung cancers. Consistent correlations were also observed for NSCLCs. Conclusions: TRAF6 might be an oncogene and the expression of TRAF6 protein is related to the progression of lung cancer. Thus, TRAF6 might become a target for diagnosis and gene therapy for lung cancer patients.