行政組織에의 組織群 生態學 接近의 必要性과 課題

주정연,박홍윤 昌原大學校 社會科學硏究所 1995 사회과학연구 Vol.2 No.-

활성형 Vitamin D₃ 전환능을 소유한 방선균에서 P-450 Hydroxylase유전자의 클로닝 전략

서주원,현창구,김정미,김승영,홍순광 명지대학교 자연과학연구소 1998 자연과학논문집 Vol.17 No.-

비타민 D₃는 포유동물이나 새의 간에서 25-hydroxyvitamin D₃[25(OH)D₃]로 전환되는데, 이 hydroxylation은 P-450 hydroxylase에 의해 촉매된다고 알려져있다. 본 연구에서는 활성형 비타민 D₃전환능을 소유한 방선균에서P-450 hydroxylase효소를 신속하게 분리가능한 PCR방법을 보고하고자 한다. Primer는 방선균에서 보고된 P-450 hydroxylase들 사이의 아미노산 서열의비교를 통해 산소결합부위와 heme-ligand pocket 주의에서 제작되었고,5종의 방선균에서 효과적으로 PCR증폭산물을 획득할 수 있었다. 증폭산물을 획득할 수 있었다.증폭 산물들의 아미노산서열을 분석한 결과 기존에 밝혀진 많은P-450 hydroxylase유전자들과 상동성을 보이고 있었으며, 특히 Streptomyces griseolus에서 분리되어 sulfonylurea 등의 제초제의 분해능력을 가진 SuaC, SubC 효소 등과 높은 아미노산 상동성을 지니고 있었다. 이러한 PCR전략은 방선균에서 유용한 물질의 대사에 관여하는 P-450 hydroxylase효소의 클로닝에 도움을 줄 것이라 사료된다 In mammals and birds, vitamin D₃is converted to 25-hydroxyvitamin D₃[25(OH)D₃] and then to 1α25-dihydroxyvitamin D₃[25(OH)₂D₃]in the liver and kideny, respectively these hydroxylating reactions are known to be catalyzed by P-450 hydroxylases. In this paper we report a PCR method which can be used for the repid amplification of DNA fragments for _450 hydroxylase from actinomycetes which can convert to active from of vitamin D₃. Primers were designed based on several regions sharing strong similarities in amino acid sequence of P-450 hydroxylases from a variety of actinomycetes, primarily in the regions of an oxygen binding site and a heme ligand pocket. These primers were used to amplify DNA fragments from five different actinomycetes. The deduced amino acid sequences of the isolated fragments revealed significant similarities to known P-450 hydroxylase including the product of the suaC or subC genes from Streptomyces griseolus that is capable of metabolizing a number of sulfonylurea herbicides and to the product of the P450sca2 from S. carbophilus that produces a specific HMG-CoA reductase inhibitor. This method will be helpful for the researchers in cloning the genes for P-450 hydroxylase involved in the biosynthesis of useful compounds.

황정(黃精) 추출물의 화학구조결정에 관한 연구 (Ⅰ)

윤중호,박주희,김정주,권기락,안철진,주우홍,강진호,신동수 Institute of Genetic Engineering Changwon National 1998 Gene and Protein Vol.2 No.1

본 연구에서는 황정 속에 포함되어 있는 생리활성 물질을 hexane, CHCl₃과 n-butanol층에서 각각의 성분들을 추출하였고, hexane층에서 분리된 화합물 I과 H 중에서, 화합물 I의 봐학구조를 ¹H-nmr, ??, DEPT135, COSY, HMQC, HMBC 스펙트럼 및 MS 스펙트럼 등의 분광학적인 방법에 의해 결정하였다. 화합물 I의 구조는 9,12-(9E, 12E)-octadecadienoic acid 임을 확인하였다. In this Paper, biologically active compounds were extracted using organic solvents as hexane. CHC1₃, n-butanol to give each component. Chemical structure of compound I was characterized using ¹H-nmr, ??, DEPT135, COSY, HMQC, HMBC spectrum and MS-spectrum, in separated compound I and Ⅱ from hexane layer. Finally, chemical structure of compound I was determined as 9,12-(9E,12E)-octadecadienoic acid.

조기탈형을 위한 라텍스 개질 초속경 콘크리트의 개발 및 강도발현 특성

이봉학,홍창우,이주형,정원경 강원대학교 석재복합신소재제품연구센터 2001 석재연 논문집 Vol.6 No.-

현재 스틸렌-부타디엔 라텍스(styrene-butadiene latex)를 혼입한 콘크리트는 광범위하게 사용되고 있다. 교면포장용으로 라텍스 개질 콘크리트(LMC)의 사용은 일반 콘크리트와 다르지 않지만 라텍스에 의해 콘크리트의 특성을 향상시킨다. 그러나, 이와 같은 라텍스 개질 콘크리트와 다르지 않지만 라텍스에 의해 콘크리트의 특성을 향상시킨다. 그러나, 이와 같은 라텍스 개질 콘크리트는 장시간 양생을 요하는 문제로 인해 긴급을 요하는 곳에는 적용할 수 없다. 따라서 본 연구에서는 초속경시멘트를 사용한 라텍스 개질 콘크리트를 개발하고, 이에 따른 작업성 및 강도발현특성을 분석하는데 그 목적이 있다. 실험결과, 지연제와 소포제를 사용한 라텍스 개질 초속경 콘크리트(RSLMC)의 강도발현을 확인할 수 있었으며, 제안된 최적배합은 초속경성의 강도발현으로 인해 현장에서 긴급보수에 적용이 가능함을 알 수 있었다. 따라서 라텍스 개질 초속경 콘크리트는 교면포장, 포장체, 빌딩등의 보수에 적용이 가능할 것으로 판단된다. Concrete containing styrene-butadiene latex is widely used, nowadays, as a protective system for bridge. The usage of latex modified concrete (LMC) as an overlay system is not all that different from any quality concrete, but the improved properties impart to the concrete by the latex. However, this may not be used for repair works because the bridge lane could not be closed for several weeks for curing. Therefore, this study focused on the development of rapid-setting cement latex modified concrete (RSLMC), and on the investigation of workability and strength development. As a result, this study clearly validated the strength development of RSLMC by introducing a retarder and an antifoam agent. The proposed optimized concrete mixtures might be satisfactorily applied for the repair works in fields, because of its rapid strength development, respectively. Therefore, RSLMC could be used in many repair or overlay works such as bridge deck, pavement or building.

Case Report : A Case of Jejunal Adenocarcinoma Diagnosed by Preoperative Double Balloon Enteroscopy

( Hong Joo Lee ),( Jae Myung Cha ),( Joung Il Lee ),( Kwang Ro Joo ),( Sung Won Jung ),( Hyun Phil Shin ) The Editorial Office of Gut and Liver 2009 Gut and Liver Vol.3 No.4

Despite a thorough history, physical examination, and complete diagnostic workup, the correct diagnosis of small-intestinal malignancy is established preoperatively in only 50% of cases; an exploratory laparotomy is often required. However, recent advances in endoscopic technologies, such as double-balloon enteroscopy (DBE), have been shown to facilitate the preoperative diagnosis of these tumors. Confirmation of malignancy using DBE in equivocal cases may greatly increase both patients` acceptance of surgery and the confidence of the physician planning a surgical resection. We describe herein the case of a 53-year-old woman with a stage I jejunal adenocarcinoma that was diagnosed by DBE and treated by laparoscopic jejunectomy. Histopathological examination revealed a stage I jejunal adenocarcinoma (T2N0M0) without muscularis propria invasion, lymphovascular invasion, or lymph-node metastasis. (Gut and Liver 2009;3:311-314)

Mitoribosome insufficiency in β cells is associated with type 2 diabetes-like islet failure

Hong Hyun Jung,Joung Kyong Hye,Kim Yong Kyung,Choi Min Jeong,Kang Seul Gi,Kim Jung Tae,Kang Yea Eun,Chang Joon Young,Moon Joon Ho,Jun Sangmi,Ro Hyun-Joo,Lee Yujeong,Kim Hyeongseok,Park Jae-Hyung,Kang 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Genetic variations in mitoribosomal subunits and mitochondrial transcription factors are related to type 2 diabetes. However, the role of islet mitoribosomes in the development of type 2 diabetes has not been determined. We investigated the effects of the mitoribosomal gene on β-cell function and glucose homeostasis. Mitoribosomal gene expression was analyzed in datasets from the NCBI GEO website (GSE25724, GSE76894, and GSE76895) and the European Nucleotide Archive (ERP017126), which contain the transcriptomes of type 2 diabetic and nondiabetic organ donors. We found deregulation of most mitoribosomal genes in islets from individuals with type 2 diabetes, including partial downregulation of CRIF1. The phenotypes of haploinsufficiency in a single mitoribosomal gene were examined using β-cell-specific Crif1 (Mrpl59) heterozygous-deficient mice. Crif1beta+/− mice had normal glucose tolerance, but their islets showed a loss of first-phase glucose-stimulated insulin secretion. They also showed increased β-cell mass associated with higher expression of Reg family genes. However, Crif1beta+/− mice showed earlier islet failure in response to high-fat feeding, which was exacerbated by aging. Haploinsufficiency of a single mitoribosomal gene predisposes rodents to glucose intolerance, which resembles the early stages of type 2 diabetes in humans.

Focal transient ischemia increases APP-BP1 expression in neural progenitor cells

Joo, Yuyoung,Lee, Sang Hyung,Ha, Sungji,Hong, Bo-Hyun,Kim, Joung-Hun,Suh, Yoo-Hun,Kim, Hye-Sun Lippincott Williams Wilkins, Inc. 2011 NEUROREPORT - Vol.22 No.5

Amyloid precursor protein binding protein-1 (APP-BP1) binds to the carboxyl terminus of APP. In this study, we explored whether APP-BP1 expression is affected by focal transient cerebral ischemia induced by middle cerebral artery occlusion in Wistar rats. APP-BP1 expression was increased in the dentate gyrus of the hippocampus and in the subventricular zone of rats exposed to focal transient cerebral ischemia. In addition, APP-BP1 immunoreactivity overlapped with antidoublecortin and anti-5-bromo-2-deoxyuridine labeling. Focal transient cerebral ischemia has been reported earlier to induce neurogenesis in adult brains. The upregulation of APP-BP1 expression in neural progenitor cells after focal transient ischemia suggests that this protein contributes to the neurogenesis induced by transient ischemia and reperfusion.

Assessment of Antioxidative Capacity in Relation to Seed Traits of Rice Varieties

Hong-Keun Song,Joung-kuk Ahn,Kwang-Ho Kim,Sun-Joo Lee,Jin-Yeong Baek,Ill-Min Chung 韓國作物學會 2006 Korean journal of crop science Vol.51 No.6

In order to assess antioxidant capacity in relation to seed traits of rice (Oryza sativa L.), ninety-six varieties were examined for antioxidative activity of brown rice grain using superoxide dismutase (SOD), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and thiobarbituric acid (TBA) assays. Overall, average total activities measured by the three methods were of very wide range between 64% and 13%. Significant differences were noted depending on the variety and evaluation method. Rice varieties with foreign origin, middle maturity, colored hulls, and colorless awn exhibited statistically significant higher total activity. As for the measurements, total activity was significantly correlated with SOD (r=0.29***), DPPH (r=0.80***) and TBA (r=0.76***). Between the three activities, SOD was not positively correlated with DPPH (r=0.15*), while TBA was significantly correlated with DPPH value (r=0.51***). DPPH (55.20%) and TBA (50.36%) were significantly higher in foreign rice, while SOD activity (44.29%) was significantly higher in domestic rice. However, an average total activity was significantly higher in foreign rice (47.31%) than in domestic rice (35.92%). SOD, DPPH and TBA activities of middle maturity in maturity time were the highest total activity (44.96%) and significantly differed from the other two groups. Total activity was significantly higher in rice with a colorless awn (42.18%) than with a colored awn (35.87%).

Response of Soybean to Elevated CO₂ Concentrations and Temperatures at Two Levels of Nitrogen Application

Hong Rae Kim,Hong Keun Song,Sun Joo Lee,Seung Hyun Kim,Sang Joon Han,Joung Kuk Ahn,Ill Min Chung 韓國作物學會 2004 Korean journal of crop science Vol.49 No.2

Effects of ambient and elevated ~textrmCO2 and high temperature, and their interactions with zero and applied nitrogen supply (NN-no nitrogen and AN-applied nitrogen) were studied on soybean (Glycine max L.) in 2001. In this experiment, elevated ~textrmCO2 (650 ~mu~textrmmol.~textrmmol-1 ) and temperature (+5~circ ) increased total dry mass at final harvest by 125% and 119% and seed weight per plant by 57% and 105% for NN and AN plants, respectively. Although the influence of temperature and temperature x ~textrmCO2 were not significant, the influences of ~textrmCO2 concentration and temperature x ~textrmCO2 concentration were significant on total dry weight and seed weight, respectively. In particular, seed weight per plant was increased, while weight per one hundred seed weight was decreased with elevated ~textrmCO2 and temperature. The N supply increased biomass and seed weight per soybean plants. The results of this study suggest that the long-term adaptation of soybean growth at an elevated ~textrmCO2 concentration and high temperature might potentially result in a increase in dry matter production and yield.

소화기암 가족력이 소화기암 발생에 미치는 위험 평가

정주원 ( Joo Won Chung ),박재준 ( Jae Jun Park ),임윤정 ( Yun Jeong Lim ),이준 ( Jun Lee ),김선문 ( Sun Moon Kim ),한정호 ( Joung-ho Han ),전성란 ( Seong Ran Jeon ),이홍섭 ( Hong Sub Lee ),김용성 ( Yong Sung Kim ),송시영 ( Si You 대한소화기학회 2018 대한소화기학회지 Vol.71 No.6

Background/Aims: This study was performed to evaluate the relationship between family history of gastrointestinal (GI) cancers and incidence of any GI cancer in the Korean population. Methods: Between January 2015 and July 2016, 711 GI cancer patients and 849 controls in 16 hospitals in Korea were enrolled. Personal medical histories, life styles, and family history of GI cancers were collected via questionnaire. Results: There was a significant difference in the incidence of family history of GI cancer between GI cancer patients and controls (p=0.002). Patients with family history of GI cancer tended to be diagnosed as GI cancer at younger age than those without family history (p=0.016). The family members of GI cancer patients who were diagnosed before 50 years of age were more frequently diagnosed as GI cancer before the age of 50 years (p=0.017). After adjusting for major confounding factors, age (adjusted odds ratio [AOR] 1.065, 95% confidence interval [CI]; 1.053-1.076), male gender (AOR 2.270, 95% CI; 1.618-3.184), smoking (AOR 1.570, 95% CI; 1.130-2.182), and sibling’s history of GI cancer (AOR 1.973, 95% CI; 1.246-3.126) remained independently associated with GI cancers. Conclusions: GI cancer patients tended to have a first relative with a history of concordant GI cancer. Personal factors (old age and male) and lifestyle (smoking) contribute to the development of GI cancer, independently. Individuals with high risk for GI cancers may be advised to undergo screening at an earlier age. (Korean J Gastroenterol 2018;71:338-348)