고압-저온 보관에 따른 쥐 치아 치주인대세포의 활성도 평가

정진호,김진,최성호,김의성,박지용,이승종 大韓齒科保存學會 2010 Restorative Dentistry & Endodontics Vol.35 No.4

본 연구의 목적은 흰 쥐의 상악 대구치를 발거한 후 치주인대세포를 0°C/2 MPa고압-저온하에 1주간 보관시켜 MTT, WST-1 검색법을 이용하여 측정한 치주인대세포의 활성도를 저속 냉동법 (No Additional Pressure. 2,3 MPa). 급속 냉동법, (No Additional Pressure. 2 MPa), -5°C/90 MPa초고압 저온보존법과 비교하여 평가하는 것이다. 생후 4주된 암컷 Sprague-Dawley계 흰쥐의 상악 좌우 제 1,2대 구치를 발거하여 각 군 당 12개의 쥐 치아를 MTT, WST-1 검색에 이용하였다 실험군은 9개 군으로 대조군은 즉시 발치군이며, 각각 3 MPa. 2 MPa. NoAdditional Pressure (NAP)의 압력을 가한 후 4°C 에 서 -35°C 까지 -0.5°C/min 속도로 서서히 냉동시킨 뒤 -196°C 에 냉동한 저속 냉동군, 발치 후 동해방지제 처리과정을 거쳐 각각 2 MPa, NP의 압력을 가한 후, -196°c 의 액화질소에 넣어 냉동한 급속 냉동군, 발치 후 각각 2MPa,NP의 압력을 가한 후. 0°C에 보관한 저온 보존군, -5°C/90 MPa의 초고압 저온 보존군으로 나누었다. 보존액은 Fmedium을 사용했으며 통해방지제로 10% dimethylsulfoxide (DMSO) 를 사용하였다. 치근변을 단위면적으로 표준화하기 위해 MTT. WST-1 측정값을 Eosin 염색 후 530 nm에서 측정한 흡광도 값으로 나누었다. 통계 분석을 위해 one way ANOVA를 시행하였으며 사후 검정으로는 Tukey HSD 방법을 사용하였고 결과는 다음과 같다. 1. MTT 검색법 및 WST-1 검색법 결과 0°C/2 MPa 고압 저온 보존군이 즉시 발치군보다 세포 활성도가 낮았으나 통계적 유의차는 없었으며, 저속 압력 냉동군 (NP, 2 MPa, 3 MPa)과, 급속 압력 냉동군 (NP, 2 MPa). 저온보존군(0 °C/NP) 초고압 저온 보존군 (-5°C /90 MPa) 보다 통계적으로 유의차있게 높은 세포 활성도를 나타내었다 (p <0.05). 2. MTT검색법 및 WST-1 검색법 결과 -5°C/90 MPa 초고압 저온 보존군이 가장 낮은 세포 활성도를 나타내었으며, MTT 검사 결과에서는 모든 군에 대해 통계적으로 유의성 있는 결과를 보였다 (p <0.05). 위의 결과를 통해, 0°C/2 MPa (20기압) 의 고압 저온 보존법이 다른 급속 냉동 보관법 (2 MPa. NAP) 이 나 저속냉동보관법 (3. 2 MPa, NAP), -5°C/90 MPa 초고압 저온 보존법에 비해 우수한 쥐 치아의 치주인대세포의 활성도를 보여 차후 치아의 재이식시 치아보관을위한방법으로의 가능성을제시하였다. The purpose of this study was to evaluate the viability of periodontalligament cells of rat teeth after low temperature preservation under high pressure by means ofMTT assay, WST-1 assay. 12 teeth of SpragueDawley white female rats of 4 week-old were used for each group. Both side of the first and second maxillary molars were extracted as atraumatically as possible under tiletamine anesthesia. The experimental groups were group 1 (Immediate extraction) , group 2 (Slow freez ing under pressure of 3 MPa), goup 3 (Slow freezing under pressure of 2 MPa), group 4 (Slow freezing under no additional pressure), group 5 (Rapid freezing in liquid nitrogen under pressure of 2 MPa), group 6 (Rapid freezing in liquid nitrogen under no additional pressure), group 7 (low-temperature preservation at Ot under pressure of 2 MPa), group 8 (low-temperature preservation at 0˚C under no additional pres sure), group 9 (low-temperature preservation at -5 t under pressure of 90 MPa). F-medium and 10% DMSO were used as preservation medium and cryo-protectant. For cryo-preservation groups, thawing was performed in 37˚C water bath, then MTT assay , WST-1 assay were processed. One way ANOVA and Tukey HSD method were performed at the 95% level of confidence. The values of optical density obtained by MTT assay and WST-1 were divided by the values of eosin staining for tissue volume standardization. In both MTT and WST-1 assay, group 7 (0˚C/2 MPa) showed higher viability of periodontal ligament cells than other group (2-6, 8) and this was statistically significant (p <0.05), but showed lower viability than group 1, immediate extraction group (no statistical significance). By the results of this study, low-temperature preservation at Ot under pressure of 2 MPa suggest the possibility for long term preservation of teeth.

Subacute oral toxicity of Dendropanax morbiferus H.Lév leaf extracts in Sprague-Dawley rats

Jiyong Park(Jiyong Park),Chang-Soo Cho(Chang-Soo Cho),Jun-Ho Song(Jun-Ho Song),Kwang Il Park(Kwang Il Park),Yeung Bae Jin(Yeung Bae Jin),Woo Hyun Kim(Woo Hyun Kim),Suk Kim(Suk Kim),Hu-Jang Lee(Hu-Jang 한국예방수의학회 2023 예방수의학회지 Vol.47 No.1

This study examined the subacute oral toxicity of Dendropanax morbiferus H.Lév leaves hot-water extracts (DMWE) using male and female Spargue-Dawley rats. Rats were orally administered the DMWE at dose levels of 0, 250, 500, 1,000, and 2,000 mg/kg body weight (BW) for four weeks. For experimental period, clinical signs and the number of deaths were examined, and feed intake and BW of all experimental animals were measured once a week for four weeks. At the end of the experiment, blood samples were collected from all rats, and all animals were euthanized and autopsies were performed to collect major organs. No dead animals were found during the experimental period. In addition, no differences were found between control and DMWE-treated groups in feed intakes, BW changes, organ weights, clinical signs, hematological parameters, and serum biochemical parameters. The results of this study provided evidence that oral administration of DMWE at the dose of 2,000 mg/kg BW is safe in rats and may not exert severe toxic effects.

Transplantation of mesenchymal stem cells within a poly(lactide-co-epsilon-caprolactone) scaffold improves cardiac function in a rat myocardial infarction model.

Jin, Jiyong,Jeong, Sung In,Shin, Young Min,Lim, Kwang Suk,Shin, Heung soo,Lee, Young Moo,Koh, Hyun Chul,Kim, Kyung-Soo Elsevier Science 2009 European journal of heart failure Vol.11 No.2

<P>AIMS: Cardiac tissue engineering has been proposed as an appropriate method to repair myocardial infarction (MI). Evidence suggests that a cell with scaffold combination was more effective than a cell-only implant. Nevertheless, to date, there has been no research into elastic biodegradable poly(lactide-co-epsilon-caprolactone) (PLCL) scaffolds. The aim of this study was to investigate the effect of mesenchymal stem cells (MSCs) with elastic biodegradable PLCL scaffold transplants in a rat MI model. METHODS AND RESULTS: Ten days after inducing MI through the cryoinjury method, a saline control, MSC, PLCL scaffold, or MSC-seeded PLCL scaffold was transplanted onto the hearts. Four weeks after transplantation, cardiac function and histology were evaluated. Transplanted MSCs survived and differentiated into cardiomyocytes in the injured region. Left ventricular ejection fraction in the MSC+PLCL group increased by 23% compared with that in the saline group; it was also higher in the MSC group. The infarct area in the MSC+PLCL group was decreased by 29% compared with that in the saline group; it was also reduced in the MSC group. CONCLUSION: Mesenchymal stem cells plus PLCL should be an excellent combination for cardiac tissue engineering.</P>

Chronological changes in epidemiologic features of patients with gallstones over the last 20 years in a single large-volume Korean center

Jiyong Zhao,Hongbeom Kim,Youngmin Han,Yoo Jin Choi,Yoonhyeong Byun,Wooil Kwon,Jin-Young Jang 대한외과학회 2019 Annals of Surgical Treatment and Research(ASRT) Vol.97 No.3

Purpose: South Korea has a high prevalence of gallstones, the type of which could be influenced by changes in diet and socioeconomic status. Here we aimed to investigate the epidemiological characteristics and changing patterns of gallstones over the past 20 years in Korea. Methods: A total of 5,808 patients who underwent cholecystectomy due to gallstones at Seoul National University Hospital between 1996 and 2015 were analyzed. Patients were divided into 4 subgroups: period 1 (1996–2000, n = 792), period 2 (2001–2005, n = 1,215), period 3 (2006–2010, n = 1,525), period 4 (2011–2015, n = 2,276). Gallstones were classified by type: pure cholesterol (PC), mixed cholesterol (MC), calcium bilirubinate (CB), black pigment (BP), and combination (COM). Results: The female to male ratio was 1.16 with mean ages of 53.6 and 55.3 years old, respectively. The ratio of cholesterol stones to pigment stones was 0.96:1. The mean age and male to female ratio of the patients increased over time. The proportion of cholesterol vs pigment stone did not differ significantly. Proportions of PC and MC stone subtypes did not change notably, whereas proportion of BP stones increased (34.0% to 45.5%), and CB stones decreased (20.7% to 5.3%). Conclusion: Gallstone types and occurrences were affected by environmental changes, and pigment stones remained common in Korea. Although no distinct increase in cholesterol stones was noted, the proportion of CB stones decreased. As the mean age at gallstone presentation increases, BP stones could become more prevalent.

Electrical Automatic Control System Based on the Internet of Things

Jiyong Jin 한국정보처리학회 2022 Journal of information processing systems Vol.18 No.6

Grid-connected distributed power generation has been widely used in green energy generation. However, dueto the distributed characteristics, distributed power generation is difficult to be dynamically allocated andmonitored in the electrical control process. In order to solve this problem, this research combined the Internetof Things (IoT) with the automatic control system of electrical engineering to improve the control strategy ofthe power grid inverter according to the characteristics of the IoT system. In the research, a connection systemof the power grid inverter and the IoT controller were designed, and the application effect was tested bysimulation experiments. The results showed that the power grid inverter had strong tracking control ability forcurrent and power control. Meanwhile, the electrical control system of the IoT could independently anddynamically control the three-phase current and power. The given value was reached within 50 ms after thestep signal was input, which could protect the power grid from being affected by the current. The overall systemcould realize effective control, dynamic control and protective control.

Development of an autonomous outdoor patrol robot in private road environment

Jiyong Jin,Changbae Jung,Donghyeon Kim,Woojin Chung 제어로봇시스템학회 2010 제어로봇시스템학회 국제학술대회 논문집 Vol.2010 No.10

Recently, the necessity of an autonomous outdoor patrol robot is increasing. The outdoor navigation is more difficult than an indoor navigation because the outdoor environment is more complex than the indoor environment. Various methods[1-2] for the outdoor navigation were researched. However, it is not easy to implement these methods[1-2], since these solutions require expensive sensors. In this paper, we compute the robot’s pose by using Extended Kalman Filter[3], and extract the road information by using single Laser Range Finder(LRF). The localization, motion control, and the road information are used for a stable navigation. By using the proposed method, the autonomous outdoor patrol robot can navigate successfully in regular road environments.

Motion Control of Indoor Mobile Robots for Safe navigation in Cluttered Environment

Jiyong Jin,Woojin Chung 제어로봇시스템학회 2013 제어로봇시스템학회 국제학술대회 논문집 Vol.2013 No.10

실내 자율주행로봇의 운동 불확실성을 고려한 장애물 회피 기법

진지용(Jiyong Jin),정우진(Woojin Chung) 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.12

이륜이동로봇의 충돌위험을 고려한 운동제어기법

진지용(Jiyong Jin),정우진(Woojin Chung) 한국정밀공학회 2011 한국정밀공학회 학술발표대회 논문집 Vol.2011 No.6월

Isolation and characterization of tumorspheres from a recurrent pineoblastoma patient: Feasibility of a patient-derived xenograft

Kwak, Jiyong,Shim, Jin-Kyoung,Kim, Dong Seok,Lee, Ji-Hyun,Choi, Junjeong,Park, Junseong,Shin, Kyoung-Jin,Kim, Se-Hoon,Kim, Pilnam,Huh, Yong-Min,Kim, Eui Hyun,Chang, Jong Hee,Kim, Sun Ho,Kang, Seok-Gu Spandidos Publications 2016 International journal of oncology Vol.49 No.2

<P>The existence of tumorspheres (TSs) might confer treatment resistance to pineoblastoma (PB). The existence of PB TSs with cellular immortalization potential has not yet been reported. We developed a procedure for isolating TSs from recurrent PB (rPB) and tested whether their properties made them suitable for use as a patient-derived xenograft (PDX). Immunocytochemical staining, RT-PCR and quantitative real-time PCR showed that, among stemness proteins, CD133, musashi and podoplanin were expressed at elevated levels in rPB TSs, but nestin was not. rPB TSs cultured under neuro-glial differentiation conditions expressed TUBB3, but not GFAP, MBP or NeuN. Unlike glioblastoma TSs, rPB TSs showed no clear evidence of invasion in 3D invasion assay or increased expression of genes associated with epithelial-mesenchymal transition. An orthotopic xenograft showed that tumor xenografts replicated the histopathological features of the patient tumor and expressed similar genome profiles, as determined by short tandem repeat genotyping. These data demonstrate the isolation and the characterization of rPB TSs for the first time. Using an orthotopic xenograft, we showed that rPB TSs could replicate the patient tumor, demonstrating their potential as a PDX for precision medicine.</P>