國內에 植栽되어 있는 外國産樹種의 特性調査(Ⅰ) : 外來樹種調査 The exotic woody plants

黃震聲,文永培 진주산업대학교 1980 論文集 Vol.18 No.-

The exotic woody plants which were surveyed until 1979 belonged to 174 species and 110 families. Among these woody plants introduced the most of species were 134 species of Rosaceae, and the next rank; 130 species of Pinaceae, 110 species of Cupressaceae, 81 species of Leguminosa, 51 species of Aceraceae, 49 species of Berberidaceae, 49 species of Aguifoliaceae, and yet 19 families had only I species. In each country of origin there were total 76 countries. Among all sorts of exotic woody plants surveyed 320 species were introduced from China, 210 from the North America, 190 from Japan, and the countries above 30 species were Newzealand, Taiwan, Himalaya, Chile, Australia, Mexico and India etc. and the countries introduced by only 1 species were 24. On the possession of those trees of today Cheonlipo botanic garden have been kept the most 723 species and the next nursery garden of Seoul city office, fo rest experiment station.

播種期와 栽植密度가 大豆收量에 미치는 影響

文永培,金鎭雨 진주산업대학교 1980 論文集 Vol.18 No.-

大豆의 單作栽培(早播區)와 麥後作栽培(晩播區)에 있어서 栽植密度를 各各 달리 했을때 大豆의 生育 및 收量差를 調査하므로서 作付體係에 따른 栽植密度를 究明하기 위하여 本試驗을 實施한바 몇가지 結果를 얻어 要約하고져 한다. 1. 播種期間의 開花에 所要된 日數는 單作早播區(5月 20日 播種區)가 59日, 晩播區(6月 20日 播種區)는 38日이 소요되어 晩播할수록 開花日數가 短縮되었고 成熱日數도 역시 같은 傾向이었다. 2. 播種期에 관계없이 密植일수록 莖長을 길어지고 反面 分枝數는 적은 傾向이었다. 3. 收量에 있어서는 早播區가 晩播區에 比하여 增收되었고 특히 早播區는 疎植할수록, 晩播區는 密植에 가까울수록 增收되는 結果를 보였다. This experiment was conducted to determine the feasible population density of soybean treated with the different population densities and sowned in the two difference seasons in the early spring and in the late spring after harvest of wheat and barley and the results were as follows; 1. The reguiring days to flowering of the soybean took 59 days in the treatment sowned in the early spring and 38 days in the treatment sowned in the late spring. The later we planted, the shorter the days to flowering became. In the case in the days to maturity, it showed similar trends in response to the days to flowering. 2. The more the populations increased, the longer the length of leaf became, but the number of branches became, shorter and shorter. However, it had nothing to do with the different sowing season. 3. In the treatment sowned in the early spring, the yields of soybean was increased than those in the late spring. Especially, the more the population density in the early spring and the less the population density in the late spring increased, the more the yield was high.

Establishment of withdrawal time and analysis of didecyldimethylammonium chloride (DDAC) residue in edible tissues of broiler chickens by spraying DDAC disinfectant

Yeung Bae Jin,Woo H. Kim,Kwang Il Park,Sung Joong Lee,Suk Kim,Hu-Jang Lee 한국예방수의학회 2023 예방수의학회지 Vol.47 No.4

This study was conducted to reset the maximum residue limit (MRL) for didecyldimethylammonium chloride (DDAC) in broiler chickens. The disinfectant containing DDAC (10%, w/w) was diluted 160 times and evenly sprayed on the bodies of twenty-four broiler chickens at a rate of 15 mL per day per bird for 7 days. After the disinfectant treatment, tissue samples were collected from six broiler chickens at 0.25, 1, 3 and 5 days, respectively. Residual DDAC concentrations in poultry tissues were determined using LC-MS/MS. Correlation coefficient (0.99 >), the limits quantification (LOQ, 2.0~10.0 μg/kg), recoveries (86.9~118.6%), and coefficient of variations (<19.98%) were satisfied the validation criteria of Korean Ministry of Food and Drug Safety. In all tissues except for liver, DDAC was detected more than LOQ at 5 days after the disinfectant treatment. In liver tissues, DDAC was detected more than LOQ at 3 days after treatment. According to the European Medicines Agency’s guideline on determination of withdrawal periods, withdrawal period of DDAC in poultry tissues was established to 26 days. In conclusion, the developed analytical method is sensitive and reliable for detecting DDAC in poultry tissues. When DDAC disinfectant is sprayed on a poultry house in the presence of broiler chickens, it is necessary to keep the disinfectant from contacting the body of the livestock.

Effects of simultaneous combined exposure to CDMA and WCDMA electromagnetic fields on serum hormone levels in rats

Jin, Yeung Bae,Choi, Hyung-Do,Kim, Byung Chan,Pack, Jeong-Ki,Kim, Nam,Lee, Yun-Sil Oxford University Press 2013 Journal of radiation research Vol.54 No.3

<P>Despite more than a decade of research on the endocrine system, there have been no published studies about the effects of concurrent exposure of radiofrequency electromagnetic fields (RF-EMF) on this system. The present study investigated the several parameters of the endocrine system including melatonin, thyroid stimulating hormone, stress hormone and sex hormone after code division multiple access (CDMA, 849 MHz) and wideband code division multiple access (WCDMA, 1.95 GHz) signals for simultaneous exposure in rats. Sprague-Dawley rats were exposed to RF-EMF signals for 45 min/day, 5 days/week for up to 8 weeks. The whole-body average specific absorption rate (SAR) of CDMA or WCDMA was 2.0 W/kg (total 4.0 W/kg). At 4 and 8 weeks after the experiment began, each experimental group's 40 rats (male 20, female 20) were autopsied. Exposure for 8 weeks to simultaneous CDMA and WCDMA RF did not affect serum levels in rats of melatonin, thyroid stimulating hormone (TSH), triiodothyronine (T3) and thyroxin (T4), adrenocorticotropic hormone (ACTH) and sex hormones (testosterone and estrogen) as assessed by the ELISA method.</P>

Effects on micronuclei formation of 60-Hz electromagnetic field exposure with ionizing radiation, hydrogen peroxide, or c-Myc overexpression

Jin, Yeung Bae,Kang, Ga-Young,Lee, Jae Seon,Choi, Jong-Il,Lee, Ju-Woon,Hong, Seung-Cheol,Myung, Sung Ho,Lee, Yun-Sil Informa Healthcare 2012 International Journal of Radiation Biology Vol.88 No.4

<P><I>Purpose</I>: Epidemiological studies have demonstrated a possible correlation between exposure to extremely low-frequency magnetic fields (ELF-MF) and cancer. However, this correlation has yet to be definitively confirmed by epidemiological studies. The principal objective of this study was to assess the effects of 60 Hz magnetic fields in a normal cell line system, and particularly in combination with various external factors, via micronucleus (MN) assays. <I>Materials and methods</I>: Mouse embryonic fibroblast NIH3T3 cells and human lung fibroblast WI-38 cells were exposed for 4 h to a 60 Hz, 1 mT uniform magnetic field with or without ionizing radiation (IR, 2 Gy), H<SUB>2</SUB>O<SUB>2</SUB> (100 <I>μ</I>M) and cellular myelocytomatosis oncogene (c-Myc) activation. <I>Results</I>: The results obtained showed no significant differences between the cells exposed to ELF-MF alone and the unexposed cells. Moreover, no synergistic effects were observed when ELF-MF was combined with IR, H<SUB>2</SUB>O<SUB>2</SUB>, and c-Myc activation. <I>Conclusions</I>: Our results demonstrate that ELF-MF did not enhance MN frequency by IR, H<SUB>2</SUB>O<SUB>2</SUB> and c-Myc activation.</P>

Effect of KAI1(CD82) sialylation on the metastatic behavior of human colon cancer cells

Jung-Jin Park,Minyoung Lee,Yeung Bae Jin,Yoon-Jin Lee,Young-Gyu Ko,Yun-Sil Lee 한국당과학회 2011 한국당과학회 학술대회 Vol.2011 No.1

The cell membrane glycoprotein KAI1 (CD82) is a candidate metastasis suppressor gene that has been indicated in the progression of solid tumors. KAI1 is significantly downregulated in various human malignancies. However, the expression and mechanism of regulation of KAI1 in metastasis has not yet been fully elucidated. Previously, it is suggested that N-glycosylation of KAI1 occurred at high level and lead to significant inhibition or promotion of cell motility depending on the extracellular matrix. In particular, little is known about the glycosylation of KAI1 and its functional role in cancer metastasis. Recently, we demonstrated that expression of ST6 Gal I, which was highly expressed in colon cancer, was responsible for integrin 1-mediated adhesion and migration in human colon cancer. Remarkably, KAI1 inhibits the tumor metastasis via interaction with tetraspanins, integrins and chemokines which are responsible for cell migration, adhesion and signalling. We found for the first time that KAI1 was sialylated by ST6 Gal I in human colon cancer cells and sialic acid modification was involved in stabilization of KAI1 by proteasome-dependent manner. Also, abolishment of Asn residues largely disrupts the migration-, invasion-, suppressive activities of KAI1. Importantly, when KAI1 was expressed in HCT116 human colon cancer cells with ST6 Gal I, inhibitory effects on migration and invasion was abrogated by altering of p130CAS-Crk coupling, a signaling step for integrin dependent migration. Moreover, sialylated KAI1 interacted with PKC-integrin and is unlikely to inhibit cell migration through its associated proteins. These new observations may indicated that KAI1 can be stably expressed by sialylation in the process of tumor malignancy and sialylation profoundly affects KAI1, leading to significant alterations of functions as metastasis suppressor.

Soluble HSPB1 regulates VEGF-mediated angiogenesis through their direct interaction.

Lee, Yoon-Jin,Lee, Hae-Jun,Choi, Seo-Hyun,Jin, Yeung Bae,An, Ho Jung,Kang, Jin-Hyoung,Yoon, Sam S,Lee, Yun-Sil Rapid Science Publishers ; Kluwer Academic Publish 2012 Angiogenesis Vol.15 No.2

<P>Endothelial cell function is critical for angiogenic balance in both physiological and pathological conditions, such as wound healing and cancer, respectively. We report here that soluble heat shock protein beta-1 (HSPB1) is released primarily from endothelial cells (ECs), and plays a key role in regulating angiogenic balance via direct interaction with vascular endothelial growth factor (VEGF). VEGF-mediated phosphorylation of intracellular HSPB1 inhibited the secretion of HSPB1 and their binding activity in ECs. Interestingly, co-culture of tumor ECs with tumor cells decreased HSPB1 secretion from tumor ECs, suggesting that inhibition of HSPB1 secretion allows VEGF to promote angiogenesis. Additionally, neutralization of HSPB1 in a primary mouse sarcoma model promoted tumor growth, indicating the anti-angiogenic role of soluble HSPB1. Overexpression of HSPB1 by HSPB1 adenovirus was sufficient to suppress lung metastases of CT26 colon carcinoma in vivo, while neutralization of HSPB1 promoted in vivo wound healing. While VEGF-induced regulation of angiogenesis has been studied extensively, these findings illustrate the key contribution of HSPB1-VEGF interactions in the balance between physiological and pathological angiogenesis.</P>

Chlorogenic acid alleviates the reduction of 14-3-3 protein in the cerebral cortex of stroke animal model

Murad-Ali Shah, Yeung Bae Jin, Myeong-Ok Kim, Phil-Ok Koh 한국예방수의학회 2023 예방수의학회지 Vol.47 No.3

Ischemic stroke causes severe neuronal damage. Chlorogenic acid is a phenolic substance present in fruits and coffee. It also exerts neuroprotective effects against various brain injuries. The 14-3-3 family protein perform a variety of functions including metabolism, signal transduction, cell differentiation, and apoptosis. The purpose of this study is to investigate whether chlorogenic acid regulates the expression of 14-3-3 protein in stroke animal models. Ischemic stroke was induced by middle cerebral artery occlusion (MCAO) surgery. Phosphate buffered saline (PBS) or chlorogenic acid (30 mg/kg) were intraperitoneally injected to adult male rats 2 h before MCAO surgery. Adhesive-removal test was performed 24 h after MCAO surgery and cerebral cortical tissues were collected for further study. MCAO damage caused severe neurological impairment and chlorogenic acid treatment ameliorated this disorder. Our proteomic approach showed a decrease in 14-3-3 expression in MCAO animals with PBS. The decrease in 14-3-3 expression alleviated in MCAO animal with chlorogenic acid. We confirmed changes in various 14-3-3 protein isoforms, including beta/alpha, zeta/delta, gamma, epsilon, eta, and tau through reverse transcription-PCR. These results explained that chlorogenic acid regulates the expression of 14-3-3 protein in MCAO-induced cerebral ischemia. 14-3-3 is considered to be an important protein for cell survival through binding to pro-apoptotic proteins. The maintenance of 14-3-3 levels is an important event in neuroprotection against ischemic injury. Therefore, we can demonstrate that the 14-3-3 protein contributes to the neuroprotective effect of chlorogenic acid in stroke animal models.

Establishment of withdrawal time and analysis of ethopabate residue in edible tissues of broiler chickens administered in drinking water

Jun Young Kim, Yeung Bae Jin, Woo H. Kim, Kwang Il Park, Sung Joong Lee, Suk Kim, Hu-Jang Lee 한국예방수의학회 2023 예방수의학회지 Vol.47 No.3

This study investigated ethopabate (EPB) residues in edible tissues of broiler chickens given in drinking water and established the withdrawal time (WT) of EPB in poultry tissues. Twenty-four healthy Ross broiler chickens were orally administered with EPB at the concentration of 3.8 mg/L for 14 days (EPB-1, n=24) and 15.2 mg/L for 7 days (EPB-2, n=24) through drinking water, respectively. After the drug treatment, tissue samples were collected from six broiler chickens at 0, 1, 3, and 5 days, respectively. EPB residue concentrations in poultry tissues were determined using LC-MS/MS. Correlation coefficient values ranged from 0.9980 to 0.9998, and the limits of detection and quantification (LOQ) were 0.03~0.09 and 0.1~0.3 μg/kg, respectively. Mean recoveries in muscle, liver, kidney and skin/fat tissues were 95.9~109.8, 108.7~115.3, 89.9~96.6 and 86.7~96.8%, respectively, and coefficient of variations were less than 17.11%. At the end of the drug-administration period (0 day), EPB was detected at levels under the LOQ in all tissues from both the EPB-1 and EPB-2 groups. According to the results of EPB residue in Ross broiler tissues, withdrawal periods of both EPB-1 and EPB-2 in poultry tissues were established to 0 day. In conclusion, the developed analytical method is suitable for the detection of EPB in poultry tissues, and the estimated WT of EPB in poultry tissues will contribute to ensuring the safety of Ross broiler chickens.

Establishment of withdrawal time and analysis of amprolium residue in edible tissues of broiler chickens administered in drinking water

Jun Young Kim(Jun Young Kim),Woo H. Kim(Woo H. Kim),Yeung Bae Jin(Yeung Bae Jin),Kwang Il Park(Kwang Il Park),Sung Joong Lee(Sung Joong Lee),Suk Kim(Suk Kim),Hu-Jang Lee(Hu-Jang Lee) 한국예방수의학회 2023 예방수의학회지 Vol.47 No.2

From 2020, Korean Animal and Plant Quarantine Agency has reset the withdrawal time (WT) for veterinary drugs typically used in livestock in preparation for the introduction of positive list system (PLS) program in 2024. This study was conducted to reset the MRL for amprolium (APL) in broiler chickens as a part of PLS program introduction. Forty-eight healthy Ross broiler chickens were orally administered with APL at the concentration of 60 mg/L (APL-1, n=24) for 14 days and 240 mg/L (APL-2, n=24) for 7 days through drinking water, respectively. After the drug treatment, tissue samples were collected from six broiler chickens at 0, 1, 3 and 5 days, respectively. Residual APL concentrations in poultry tissues were determined using LC-MS/MS. Correlation coefficient (0.99 >), the limits quantification (LOQ, 0.3~5.0 μg/kg), recoveries (81.5~112.4%), and coefficient of variations (<15.5%) were satisfied the validation criteria of Korean Ministry of Food and Drug Safety. In APL-1, APL in all tissues except for kidney was detected less than LOQ at 3 days after drug treatment. In APL-2, APL in liver and kidney was detected more than LOQ at 5 days after treatment. According to the European Medicines Agency’s guideline on determination of withdrawal periods, withdrawal periods of APL-1 and APL-2 in poultry tissues were established to 3 and 2 days, respectively. In conclusion, the developed analytical method is sensitive and reliable for detecting APL in poultry tissues. The estimated WT of APL in poultry tissues is longer than the current WT recommendation of 2 days for APL in broiler chickens.