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The Effect of Salinity on Starch-Derived Metabolites Released from Kimchi During Fermentation
Jinsol Song,Ju Hun Lee,Duyun Jeong,Hyun-Jung Chung 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
This study was carried out to identify and quantify sugar-related metabolites and oligosaccharides released from kimchi depending on salinities during fermentation. The kimchi adjusted to 1.0, 2.5, and 4.0% salinity was stored at 4℃ for 70 days. The sugar metabolites and oligomers in kimchi during fermentation were detected by using HPAEC-PAD system. Salt concentrations in kimchi highly influenced its starch-derived metabolites during fermentation. The lower salinity of kimchi induced relatively greater change in mannitol and fructose contents during fermentation. Kimchi samples with 1.0% salinity had greater increase in oligomers than other samples, indicating the rapid rate of fermentation. Total oligomer contents in kimchi reached the maximum value after 10 days of fermentation and they decreased continuously thereafter. Our results suggest that the quantities of starch-derived metabolites in kimchi during fermentation were highly influenced according to the salinity, which could provide useful information for kimchi industries.
Ji-won Jeong,Jinsol Hong,Taechul Park,SoEun Eom,Kimoon Son,Kijong Cho,Jung-Joon Park 한국응용곤충학회 2023 한국응용곤충학회 학술대회논문집 Vol.2023 No.10
Climate change and biological invasions are the greatest threats to biodiversity, agriculture, health and the global economy. Tomato leafminer(Tuta absoluta) (Meyrick) (Lepidoptera: Gelechiidae) is one of the most important threats to agriculture worldwide. This pest is characterized by rapid reproduction, strong dispersal ability, and highly overlapping of generations. Plants are damaged by direct feeding on leaves, stems, buds, calyces, young ripe fruits and by the invasion of secondary pathogens which enter through the wounds made by the pest. Since it invaded Spain in 2006, it has spread to Europe, the Mediterranean region, and, in 2010, to some countries in Central Asia and Southeast Asia. In East Asia, Tomato leafminer was first detected in China in Yili, Xinjiang Uygur Autonomous Region, in 2017. There is a possibility that this pest will invade South Korea as well. This study provides this by the use of MaxEnt algorithm for modelling the potential geographical distribution of Tomato Leafminer in South Korea Using presence-only data.
Optimization of Culture Conditions for Maintaining Pig Muscle Stem Cells In Vitro
Kwang-Hwan Choi,Ji Won Yoon,Minsu Kim,Jinsol Jeong,Minkyung Ryu,Sungkwon Park,Cheorun Jo,Chang-Kyu Lee 한국축산식품학회 2020 한국축산식품학회지 Vol.40 No.4
Muscle stem cells isolated from domestic animals, including cows and pigs, were recently spotlighted as candidates for the production of alternative protein resources, so-called cultured meat or lab-grown meat. In the present study, we aimed to optimize the in vitro culture conditions for the long-term expansion of pig muscle stem cells via the screening of various signaling molecules. Pig muscle stem cells were collected from the biceps femoris muscles of 3-d-old crossbred pigs (Landrace×Yorkshire×Duroc, LYD) and cultured in minimum essential medium-based growth media. However, the pig muscle stem cells gradually lost their proliferation ability and featured morphologies during the long-term culture over two weeks. To find suitable in vitro culture conditions for an extended period, skeletal muscle growth medium-2, including epidermal growth factor (EGF), dexamethasone, and a p38 inhibitor (SB203580), was used to support the stemness of the pig muscle stem cells. Interestingly, pig muscle stem cells were stably maintained in a long-term culture without loss of the expression of myogenic marker genes as determined by PCR analysis. Immunostaining analysis showed that the stem cells were capable of myogenic differentiation after multiple passaging. Therefore, we found that basal culture conditions containing EGF, dexamethasone, and a p38 inhibitor were suitable for maintaining pig muscle stem cells during expanded culture in vitro. This culture method may be applied for the production of cultured meat and further basic research on muscle development in the pig.