Establishment of immortalized human ovarian surface epithelial cells using the lentiviral system from Korea Gynecologic Cancer Bank

( Jinkyoung Kong ),( Hyunja Kwon ),( Eun Ju Lee ),( Ha-yeon Shin ),( Wookyeom Yang ),( Jae-hoon Kim ),( Doo Byung Chay ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-

목적: Immortalization is a key difference that distinguishes cancer cell form normal cell. In cell line studies, normal cells like human ovarian surface epithelial cells (HOSE) are difficult to culture and limited due to the senescence nature of normal cell. Thus for the expansion of biospecimen from Korea Gynecologic Cancer Bank, the purpose of this study was to establish immortalize human ovarian surface epithelial cells (IHOSE). 방법: Immortalize human ovarian surface epithelial cells (IHOSE) were established by transfecting HPV E6/E7 and SV40 T antigen to short cultured human ovarian surface epithelial cells (HOSE) using the lentiviral system. Cells were grown in Dulbecco’s Modified Eagle Medium (DMEM) in the presence of 10% fetal bovine serum (FBS) and were cultured in 5% CO2 balanced air at 37°C. After confirmation of successful passages, immortalize human ovarian surface epithelial cells (IHOSE) were analyzed for alteration of gene expressions by DNA fingerprinting method. 결과: Five new human ovarian surface epithelial cells that succeeded ten passages were established using the lentiviral system (IHOSE). For molecular expression, 6% differential gene expression was noted compared to three human ovarian surface epithelial cells (HOSE). 결론: Newly established human ovarian surface epithelial cells, from Korea Gynecologic Cancer Bank, can be important research resources for a mimic model against molecular alternation of ovarian cancer.

Cloning of Dextranase Gene from Bacteroides xylanisolvens X3 isolated from Human Intestine and Characterization of its Enzyme

Jinkyoung Kim,Ling Li,Yu Mi Son,Nam Soo Han 한국당과학회 2014 한국당과학회 학술대회 Vol.2014 No.1

백본이 수정된 YOLOv3를 이용한 약용작물 병충해 탐지 AI 서비스 구현

허진경(JinKyoung Heo),이근호(Geunho Lee) 한국정보기술학회 2022 Proceedings of KIIT Conference Vol.2022 No.12

식물이 생산하는 화학 물질은 자신의 생존을 향상하게 하는 과정 과정에서 화학 물질이 더 많이 생산될 수 있다. 특히 약용으로 사용되는 천연 추출물을 다량 함유한 약용작물은 적은 노동력으로 농업 생산성을 최대화할 수 있다. 본 논문은 식물의 천연 추출물 함량을 높일 수 있는 환경적 요인을 알아보기 위한 과정에서 발생할 수 있는 병충해로 인한 피해를 줄이기 위해 인공신경망 객체탐지 알고리즘인 YOLOv3의 백본을 수정한 모델을 적용한 인공지능 모델과 탐지 서비스 구현을 제안한다. Chemicals produced by plants can be produced more in the process of improving their own survival. In particular, medicinal crops containing a large amount of natural extracts used for medicinal purposes can maximize agricultural productivity with less labor. This paper proposes an artificial intelligence model and detection service implementation with a modified model of YOLOv3, an artificial neural network object detection algorithm, to reduce damage caused by pests in the process of identifying environmental factors that can increase the natural extract content of plants.

코메니우스의 덕성교육: 7 주덕(主德)의 현대적 가치

최진경(JinKyoung Choi) 한국기독교교육정보학회 2021 기독교교육정보 Vol.- No.70

4차산업혁명의 과학기술과 포스트휴머니즘의 도래는 인간 이해에 새로운 변화를 가져오고 있다. 이는 기독교 교육에도 큰 도전과 과제를 던져준다. 본 논문에서는 기독교적 관점에서 인간을 인간답게 하는 ‘인간다움’이라는 인간 본성의 근본 토대는 무엇인지 밝히며, 인간다움의 중요성을 일깨우고, 인간다움을 회복하는 데 있어 한국교회가 오랫동안 간과해왔던 덕성교육의 중요성과 필요성을 제기하고 있다. 이를 위해 코메니우스가 『대 교수학』(Didactica magna) 제23장에서 덕성 교육을 위해 제시한 7 덕목의 의미와 가치를 다루며, 우리 시대 기독교 교육의 실천적 과제로써 덕성교육의 필요성과 시급성을 피력하였다. The Science and Thechnology of the 4th Industrial Revolution and post-humanism are foretelling a big change different from before in Human understanding. This gives great challenge and task to Christian Education as well. In this paper is revealed, in Christian point of view, what is the fundamental foundation of human nature called ‘human-becoming’ that makes humans human-being, and is awakened the importance of ‘human-becoming’. This paper is raised also the importance and necessity of virtue education, which the Korean Church had long overlooked. Therefore, this thesis deals with the meaning and value of the 7 virtues that Comenius suggested for virtue education in the Chapter 23 of the “Didactica magna”, and emphasize the importance and urgency of virtue education as a practical task for Christian education in our time.

Metal-Lined Semiconductor Nanotubes for Surface Plasmon-Mediated Luminescence Enhancement

Yoo, Jinkyoung,Ma, Xuedan,Tang, Wei,Yi, Gyu-Chul American Chemical Society 2013 NANO LETTERS Vol.13 No.5

<P>Highly efficient solid-state light-emitting devices require semiconductor architectures equipped with high quantum efficiency and integratability on conductive substrates. Surface plasmon (SP)-mediated luminescence enhancement has been considered as one of the most promising solutions, because SP resonance can greatly improve the radiative recombination rate and be achieved using metal entities compatible with the electrode fabrication process. Nevertheless, metal/semiconductor heterostructures have had several fabrication-compatible issues due to metal as a potential contaminant of the semiconductor. We present here a simple fabrication scheme for a metal-lined semiconductor nanotube heterostructure, in which a metal layer is selectively formed on the inner wall of the semiconductor nanotube. The Ag-lining process in a ZnO nanotube resulted in 7.5-fold enhancement of the photoluminescence intensity at 11 K. This SP fabrication technique looks promising for highly efficient solid-state lighting based on semiconductor nanostructures without detrimental effects.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2013/nalefd.2013.13.issue-5/nl400547z/production/images/medium/nl-2013-00547z_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl400547z'>ACS Electronic Supporting Info</A></P>

Analysis of Korean NEG-Morphemes in Terms of Transitivity

Kim, Jinkyoung 한국중앙영어영문학회 2009 영어영문학연구 Vol.51 No.1

This study analyzes the distribution and interpretations of negative forms in Korean from functionalists’ viewpoint of language, because they are heavily influenced by functions associated with constituents in a sentence/discourse. This study establishes a functional and discourse approach in the analysis of negation under the assumption that the interpretation of negation in Korean can be better explained by their functions in discourse. On the basis of this assumption, the distribution and interpretation of NEG-forms are examined in terms of transitivity. In addition, to examine why the two NEG-morphemes are so distributed, a transitivity theory is applied. In this theory, an can be regarded as an unmarked NEG-morpheme which negates predicates of either high or low transitivity, but mot must be regarded as a marked NEG-morpheme and is specialized to negate predicates of high transitivity only. The semantic interpretations of the NEG-morphemes an and mot then follow naturally as a consequence of derivation from the degree of transitivity each NEG-morpheme is associated with in the given predicate. In short, this study shows that the distribution and interpretation of NEG-forms cannot be explained in terms of purely syntactic form, but they can be explained better in terms of their functions in discourse.

Fabrication and Optical Characteristics of Position-Controlled ZnO Nanotubes and ZnO/Zn_0.8Mg_0.2O Coaxial Nanotube Quantum Structure Arrays

Yoo, Jinkyoung,Hong, Young Joon,Jung, Hye Seong,Kim, Yong-Jin,Lee, Chul-Ho,Cho, Jeonghui,Doh, Yong-Joo,Dang, Le Si,Park, Kyung Ho,Yi, Gyu-Chul WILEY-VCH Verlag 2009 Advanced Functional Materials Vol.19 No.10

<P>The position-controlled growth and structural and optical characteristics of ZnO nanotubes and their coaxial heterostructures are reported. To control both the shape and position of ZnO nanotubes, hole-patterned SiO<SUB>2</SUB> growth-mask layers on Si(111) substrates with GaN/AlN intermediate layers using conventional lithography are prepared. ZnO nanotubes are grown only on the hole patterns at 600 °C by catalyst-free metal–organic vapor-phase epitaxy. Furthermore, the position-controlled nanotube growth method allows the fabrication of artificial arrays of ZnO-based coaxial nanotube single-quantum-well structures (SQWs) on Si substrates. In situ heteroepitaxial growth of ZnO and Zn<SUB>0.8</SUB>Mg<SUB>0.2</SUB>O layers along the circumference of the ZnO nanotube enable an artificial formation of quantum-well arrays in a designed fashion. The structural and optical characteristics of the ZnO nanotubes and SQW arrays are also investigated using synchrotron radiation X-ray diffractometry and photoluminescence and cathodoluminescence spectroscopy.</P> <B>Graphic Abstract</B> <P>Position-controlled ZnO nanotubes and their coaxial nanotube quantum structure arrays are fabricated by selective metal–organic vapor-phase epitaxy (see image). Both the position-controlled nanotube and associated coaxial heterostructures arrays exhibit excellent luminescent characteristics. This simple, precise, well-controlled “bottom-up” fabrication provides a general and rational route to integrate vertical nanodevices for nanometer-scale electronics and optoelectronics. <img src='wiley_img/1616301X-2009-19-10-ADFM200801123-content.gif' alt='wiley_img/1616301X-2009-19-10-ADFM200801123-content'> </P>

Electrical and optical characteristics of hydrogen-plasma treated ZnO nanoneedles

Yoo, Jinkyoung,Park, Won Il,Yi, Gyu-Chul American Vacuum Society 2005 JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B - Vol.23 No.5

다문화주의와 사회통합 -캐나다와 호주를 중심으로-

박진경 ( Jinkyoung Park ),임동진 ( Dong Jin Lim ) 한국정책학회 2012 韓國政策學會報 Vol.21 No.2

다문화주의가 사회통합전략을 위한 정책아이디어로 부각되고 있지만, 국가마다 선택적으로 해석, 적용됨에 따라 사회통합 성과가 다르게 나타나고 있다. 본 연구는 정책아이디어와 다문화주의의 이론적 논의를 바탕으로 다문화주의의 형성과정과 정책수단의 특성에 따라 사회통합의 성과에 영향을 미치는 것으로 분석틀을 설정하고, 강한 다문화주의 국가로 분류되고 있는 캐나다와 호주의 사례를 통해 검증하였다. 이를 위해 첫째, 이민 및 소수인종의 등장 등 역사적 맥락에 의한 다문화주의 정책 채택 배경과 그 변화과정을 통해 국가마다 다르게 형성된 다문화주의의 이념과 목표를 분석하였다. 둘째, 다문화주의 정책수단인 법과 제도 및 정부기구, 예산, 프로그램의 차이를 분석하였다. 셋째, 두 나라의 사회통합의 성과를 중심으로 경험적 자료를 동원하여 정책성과를 분석하였다. 그 결과, 캐나다는 강한 소수인종의 등장으로 다문화주의를 국가정체성이라는 높은 수준의 가치로 받아들이고, 법과 제도 등 안정적 정책수단을 통해 가시적인 사회통합 성과를 보이고 있는 반면, 호주는 백호주의를 선호하는 정치적 영향과 낮은 대중적 지지로 동화주의에 가까운 통합정책을 구현하고 있어, 그 결과 사회통합에도 도움이 되고 있지 못함을 알 수 있었다. 결국 정책아이디어의 형성과정에 나타난 특성이 정책수단을 통한 제도화와 공고화에 영향을 미치고 이는 다시 사회통합 성과에 영향을 미치고 있음을 알 수 있었다. Despite multiculturalism’shaving emerged as a policy idea for social inclusion strategy, as each country interpreted and applied it as they liked, the performance of actual social integration might appear differently in each country. This study analyzed Canada and Australia, classified as strong multicultural countries in the world, based on theoretical discussions of multiculturalism. The study went as follows: First, it analyzed the ideology and goals of multiculturalism, varying from state to state, through policy background, and historical context, such as with the emergence of immigration and ethnic minorities, and the process of their changes. Second, the study analyzed their laws and institutions, and government agencies, budgets, and programs as multiculturalism policy instruments. Third, the study analyzed the performance of social integration, by use of empirical data. Canada is showing visible results for social integration, with the emergence of a strong ethnic, accepting multiculturalism at high value in society,as part of the national identity, and as being at the foundation of its laws and institutions. But Australia, on the other hand, has been running integration policies that are close to assimilation. Because of political authorities tending towards the White Australia policy, and because of low public support for multiculturalism policies, Australia has not achieved social integration. Their process of policy formation has influenced the institutionalization and application of their policies, which in turn has influenced the social integration there.

MANET에서 안전한 서비스 제공을 위한 On-demand 라우팅 프로토콜의 성능 비교

허진경(JinKyoung Heo),양환석(HwanSeok Yang) 한국디지털콘텐츠학회 2009 한국디지털콘텐츠학회논문지 Vol.10 No.2