Multiplex PCR for simultaneous identification of turkey, ostrich, chicken, and duck

Li, Jingmei,Hong, Yeun,Kim, Jae-Hwan,Qin, Pei,Kim, Mi-Ju,Kim, Hae-Yeong The Korean Society for Applied Biological Chemistr 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.6

To discriminate economically motivated adulteration in poultry meats and products, species-specific oligonucleotide primer sets for multiplex polymerase chain reaction (PCR) of four poultry meats were newly designed. Sequences for species-specific primers were generated based on the nucleotide variation in mitochondria cytochrome b gene through sequence comparisons. The sizes of species-specific PCR products for turkey, ostrich, chicken, and duck were 217, 330, 516, and 820 bp, respectively. By adjusting PCR conditions and the concentrations of primers, a simultaneous discrimination of four poultry meats was successfully performed. The limits of detection of both single and multiplex PCR for turkey were 0.05 and 0.005 ng for other species, respectively. The developed multiplex PCR method was successfully applied to identify the target species in 34 of poultry meat products and thus was considered to be a useful tool for monitoring poultry meat products.

Multiplex PCR for simultaneous identification of turkey, ostrich, chicken, and duck

Jingmei Li,홍연,김재환,Pei Qin,김미주,김해영 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.6

To discriminate economically motivated adulteration in poultry meats and products, species-specific oligonucleotide primer sets for multiplex polymerase chain reaction (PCR) of four poultry meats were newly designed. Sequences for species-specific primers were generated based on the nucleotide variation in mitochondria cytochrome b gene through sequence comparisons. The sizes of species-specific PCR products for turkey, ostrich, chicken, and duck were 217, 330, 516, and 820 bp, respectively. By adjusting PCR conditions and the concentrations of primers, a simultaneous discrimination of four poultry meats was successfully performed. The limits of detection of both single and multiplex PCR for turkey were 0.05 and 0.005 ng for other species, respectively. The developed multiplex PCR method was successfully applied to identify the target species in 34 of poultry meat products and thus was considered to be a useful tool for monitoring poultry meat products.

Reduction in SBPase Activity by Antisense RNA in Transgenic Rice Plants: Effect on Photosynthesis, Growth, and Biomass Allocation at Different Nitrogen Levels

Lingling Feng,Hui Li,Jingmei Jiao,Ding Li,Li Zhou,Jian Wan,Yangsheng Li 한국식물학회 2009 Journal of Plant Biology Vol.52 No.5

Rice cultivar zhonghua11 (Oryza sativa L. ssp. japonica) plants with decreased sedoheptulose-1, 7- bisphosphatase (SBPase) were obtained by transformation with the rice SBPase antisense gene under the control of the maize ubiquitin promoter. The transgenic and wild-type plants were grown at different nitrogen levels (0.1, 1, or 10 mM NH4NO3). Growth rates of the seedlings were measured by the changes in dry weight, and the photosynthetic carbon reduction activities and the potential efficiency of photosystem II were measured by CO2 assimilation and Fv/Fm, respectively. At low N, there are strong effects on growth and photosynthesis when SBPase was reduced by genetic manipulation. Decreased SBPase activity led to a decrease in the amount of starch accumulated in the leaves at all N levels and the decrease was much more prominent in low N than that in high N, but the starch allocation between shoot and root was unaltered. The analysis of chlorophyll fluorescence and SBPase activity indicated that the decrease of growth and photosynthesis at different N levels were not related to the function of PSII but to the activity of SBPase. Western blot analysis showed the content of SBPase in thylakoid membranes was much more than in the stroma fractions in transgenic plants at low N. Results suggested that low N in addition to a 34% decrease in SBPase activity is sufficient to diminish photosynthesis and limit biomass production. Decreased SBPase activity may reduce the N use efficiency of photosynthesis and growth and alter biomass allocation.

Predominant Lactic Acid Bacteria in Mukeunji, a Long-term-aged Kimchi, for Different Aging Periods

홍연,Jingmei Li,Pei Qin,이신영,김해영 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.2

The distribution of dominant lactic acid bacteria (LAB) in mukeunji was examined at different stages of long-term aging. A total of 87 isolates from 3 samples were classified into 13 groups based on sodium dodecyl sulfatepolyacrylamide gel electrophoresis whole-cell protein patterns and identified using 16S rRNA gene sequencing. The 13 LAB were also identified using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and API® strips. Weissella cibaria and Leuconostoc mesenteroides were the most abundant LAB in freshly prepared samples, followed by Lactobacillus (Lact.) curvatus and Lact. sakei, based on 16S rRNA gene sequencing. The species diversity of LAB in samples aged for 12 and 24 months was reduced over time, and Pediococcus sp. was the dominant LAB species in mukeunji samples. MALDI-TOF MS enabled rapid identification of Lactobacillus species. Identification results with similarities higher than 90% using API® strips provided the same results as 16S rRNA gene sequencing.

Characterization of Macrophage-activating Lactic Acid Bacteria Isolated from Mukeunji

김재환,Jingmei Li,Seon-Kyeong Han,Pei Qin,Jushin Kim,Yeseul Park,Shin-Young Lee,Yeun Hong,김우기,김해영 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.2

Lactic acid bacteria (LAB) were reported to comprise the majority of the bacterial population in mukeunji, a long-fermented kimchi. This current study investigated the probiotic abilities of LAB isolated from mukeunji. Forty bacterial strains from mukeunji were identified by SDS-PAGE gel patterns, which were further tested for acid and bile tolerance. An assessment of the immuneboosting effects of these strains in RAW264.7 cells was also carried out by measurement of secreted proinflammatory cytokines. Heat-killed LAB were also tested for their contribution to cytokine production because certain LAB are not durable in the digestive tract. Immune-boosting strains were further characterized by SDS-PAGE of whole-cell protein and 16S rRNA gene sequencing, resulting in the identification of Lactobacillus plantarum, L. sakei, Weissella cibaria, and Pediococcus parvulus. These data and the fact that mukeunji is highly consumed in Korea, together, highlight the need of detailed epidemiological and animal feeding studies.

Designing cactus-like Fe-P doped CoNi-S arrays as highly efficient electrocatalyst for overall water splitting

Xiaojuan Feng,Yanlong Shi,Jingmei Liang,Wei Li,Xuyuan Gou 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.118 No.-

Exploring high activity, stable and low-cost electrocatalysts are of great importance for high-efficienthydrogen production via water splitting. Herein, a cactus-like Fe, P co-doped CoNi-S arraywas constructedvia facile hydrothermal reaction and subsequently sulfuration and phosphorization process. Benefitingfrom the strong synergistic effects of different components, high conductivity of bimetallic sulfide, 3Dcactus-like architecture with rich channels for mass/charge transport, and Fe and P co-doping with electronstructure regulation, the as-prepared Fe, P-NiCo2S4/NF presents superior electrocatalytic activity withlow overpotentials of 139 and 290 mV to drive the current density of 10 and 40 mA cm2 for HER and OER,respectively. Meanwhile, it also manifests the distinguished durability for over 60 h in alkaline media. Notably, when integrating in a two-electrode electrolytic cell with Fe, P-CoNi-S, a reduced operation voltageof 1.55 V is required to reach 10 mA cm2, which is superior to precious metal-based Pt/C/NF//RuO2/NFcounterparts. Impressively, it can also be driven by sustainable solar and thermal energies motivatedStirling engine. This work offers a universal strategy for designing high-activity and durable electrocatalystsby dual doping heteroatoms and synergistic interface engineering.