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Xiliang Liu,Song Liu,Youkun Fan,Jin Qi,Xin Wang,Wei Bai,Dongliang Chen,Chengdong Xiong,Lifang Zhang 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.104 No.-
The poly(L-lactide-co-e-caprolactone) (PLCL) ureteral stent creeps and loses shape stability, increasingthe risk of stent tube dislocation. The rubbery biodegradable cross-linked PLCL networks were preparedthrough gamma irradiation under vacuum in the presence of trimethylolpropane triacrylate (TMPTA),pentaerythritol tetraacrylate (PET4A), and pentaerythritol triacrylate (PETA). At a standard sterilizationdose of 25 kGy, the gel content and network density of PLCL networks increased with increasingcrosslinking agent content (1, 3, 5, 7 wt%), and crosslinking efficiency decreased in the order ofPETA > PET4A > TMPTA. The average molecular weight (Mc ) between two crosslinks ranged from 2000to 105 g/mol. To perform the beneficial semi-interpenetrated polymer network and characterized bythe principle, the networks were processed in several doses (25, 50, 75, 100, and 125 kGy). In place ofthe Charlesby-Pinner equation, the irradiation cross-linking followed the Chen-Liu-Tang equation. ThePLCL network with 7 wt% PETA had a gel fraction of 83%, tensile strength of 34.7 MPa, and tensile setvalue as low as 5%. Furthermore, degradation in vitro was slowed down. Thus, PLCL networks with appropriateelasticity and flexibility, inherent biodegradability, and excellent biocompatibility can provide apromising alternative method for soft tissue repair engineering, such as ureteral stents.
Effect of solution properties on the interaction of 90Sr(II) with GMZ bentonite
Xin Chen,Jin Wang,Shaowei Wang,Fengliang Ma,Xiaopeng Chen,Jiaxing Li 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.11
The bentonite from gaomiaozi county (Inner Mongolia, China) (GMZ bentonite) has been selected as the candidate of backfill material in China for nuclear waste repository. Herein, the sorption of 90Sr(II) on GMZ bentonite was investigated as a function of contact time, solid content, pH, ionic strength, foreign ions, humic acid (HA) and temperature. The results indicated that the sorption of 90Sr(II) was mainly dominated by ion exchange or outer-sphere surface complexation at low pH, whereas inner-sphere surface complexation was the main sorption mechanism at high pH values. The thermodynamic parameters (ΔH0, ΔS0 and ΔG0) calculated from the temperature-dependent sorption isotherms indicated that the sorption of 90Sr(II) on GMZ bentonite was an endothermic and spontaneous process. The results provided important information for the interaction mechanism of 90Sr(II) with GMZ bentonite, and is crucial for the evaluation of GMZ bentonite as backfill material.
Microstructure Distribution and Grain Coarsening Model of GCr15 Steel in the Laser Surface Treatment
Jin‑shan Chen,Zhen‑xing Li,Ya‑jie Chu,Jie Chen, Xin‑jun Shen 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.10
The GCr15 steel was subjected to laser surface treatment using a diode laser with rectangular spot. Based on the simulatedtemperature field, the microstructure distribution of the laser hardened layer was analyzed, and an empirical equation wasproposed to predict the peak temperature at different depths. The results indicated that with the decrease of depth, the peaktemperature increased exponentially, and the peak temperature at different depths was approximately linear with the powerdensity and the reciprocal of square root of scanning speed. High peak temperature led to the coarsening of grain and thesignificant dissolution of cementite near the surface of hardened layer. At the deeper position of hardened layer, the prioraustenite grain size decreased slightly due to high nucleation rate. The cementite dissolution and coarsening occurred simultaneouslynear the surface, and the cementite dissolution process was postponed due to the significant enrichment of Cr nearthe interface of austenite and cementite. Additionally, an empirical model about grain coarsening was established, which canbe used to predict the prior austenite grain size near the surface during the laser surface treatment.
Gao, Jin-Xin,Jing, Jing,Yu, Chuan-Jin,Chen, Jie The Korean Society of Plant Pathology 2015 Plant Pathology Journal Vol.31 No.2
Curvularia lunata is an important maize foliar fungal pathogen that distributes widely in maize growing area in China, and several key pathogenic factors have been isolated. An yeast two-hybrid (Y2H) library is a very useful platform to further unravel novel pathogenic factors in C. lunata. To construct a high-quality full length-expression cDNA library from the C. lunata for application to pathogenesis-related protein-protein interaction screening, total RNA was extracted. The SMART (Switching Mechanism At 5' end of the RNA Transcript) technique was used for cDNA synthesis. Double-stranded cDNA was ligated into the pGADT7-Rec vector with Herring Testes Carrier DNA using homologous recombination method. The ligation mixture was transformed into competent yeast AH109 cells to construct the primary cDNA library. Eventually, a high qualitative library was successfully established according to an evaluation on quality. The transformation efficiency was about $6.39{\times}10^5$ transformants/$3{\mu}g$ pGADT7-Rec. The titer of the primary cDNA library was $2.5{\times}10^8cfu/mL$. The numbers for the cDNA library was $2.46{\times}10^5$. Randomly picked clones show that the recombination rate was 88.24%. Gel electrophoresis results indicated that the fragments ranged from 0.4 kb to 3.0 kb. Melanin synthesis protein Brn1 (1,3,8-hydroxynaphthalene reductase) was used as a "bait" to test the sufficiency of the Y2H library. As a result, a cDNA clone encoding VelB protein that was known to be involved in the regulation of diverse cellular processes, including control of secondary metabolism containing melanin and toxin production in many filamentous fungi was identified. Further study on the exact role of the VelB gene is underway.
( Na Chen ),( Min Jin ),( Hong Mei Qu ),( Zhi Qiang Chen ),( Zhao Li Chen ),( Zhi Gang Qiu ),( Xin Wei Wang ),( Jun Wen Li ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.2
A strain of bacterium producing antifungal antibiotic was isolated and identification of the strain was attempted. We could identify the bacterium as being a Bacillus sp., based on morphological observation, physiological characteristics, and 16S rDNA sequence analysis, thus leading us to designate the strain as Bacillus sp. AH-E-1. The strain showed potent antibiotic activity against phytopathogenic and human pathogenic fungi by inducing mycelial distortion and swelling and inhibiting spore germination. The antibiotic metabolite produced by the strain demonstrated excellent thermal and pH (2-11) stability, but was labile to autoclaving. From these results, we could find a broader antifungal activity of Bacillus genus. Isolation and characterization of the active agent produced by the strain are under progress.
Jun Wei Wu,Yu Rong Xing,Yi Bo Wen,Tian Fang Li,Quan De Feng,Xiao Ping Shang,Yun Long Li,Jin Jin Feng,Xin Xin Wang,Rong Qun Zhai,Xiang Fei He,Tao Chen,Xin Jian Liu,Jian Guo Wen 대한배뇨장애요실금학회 2016 International Neurourology Journal Vol.20 No.2
Purpose: To investigate the prevalence of spina bifida occulta (SBO) and its relationship with the presence of overactive bladder (OAB) in middle-aged and elderly people in China. Methods: A cross-sectional community-based survey was carried out at 7 communities in Zhengzhou City, China from December 15, 2013 to June 10, 2014, where residents aged over 40 years were randomly selected to participate. All of the participants underwent lumbosacral radiographic analysis and relevant laboratory tests. A questionnaire including basic information, past medical history and present illness, and the OAB symptom score was filled out by all participants. Chi-square tests and logistic regression were used for data analysis with a P-value of <0.05 denoting statistical significance. Results: A total of 1,061 subjects were qualified for the final statistical analysis (58.8±11.7 years; male, 471 [44.4%]; female, 590 [55.6%]). The overall prevalence of SBO was 15.1% (160 of 1,061): 18.3% (86 of 471) in men and 12.5% (74 of 590) in women. Among these subjects, 13.7% (145 of 1,061) had OAB: 13.2% (62 of 471) in men and 14.1% (83 of 590) in women. The results of logistic regression showed that age, SBO, history of cerebral infarction (HCI), and constipation were risk factors for OAB (P<0.05), while sex, history of childhood enuresis (HCE), body mass index (BMI), and diabetes mellitus (DM) were not (P>0.05). In men, age, SBO, and constipation were risk factors for OAB (P<0.05), while HCE, BMI, DM, HCI, and benign prostate hyperplasia were not (P>0.05). In women, age, SBO, and HCI were risk factors for OAB (P<0.05), while HCE, BMI, DM, vaginal delivery, and constipation were not (P>0.05). Conclusions: The prevalence of SBO is high and it is related to OAB in middle-aged and elderly people in China.
Triterpenoids from Schisandra henryi with Cytotoxic Effect on Leukemia and Hela Cells In Vitro
Chen, Ye-Gao,Wu, Zheng-Cai,Lv, Yu-Ping,Gui, Shi-Hong,Wen, Jin,Liao, Xin-Rong,Yuan, Li-Ming,Halaweish, Fathi The Pharmaceutical Society of Korea 2003 Archives of Pharmacal Research Vol.26 No.11
Four known lanostane triterpenoids, schiprolactone A (1), schisanlactone B (2), nigranoic acid (3) and schisandronic acid (4) Were isolated from the stems of Schisandra henryi for the first time. Their structures were characterized by IR, MS and NMR techniques. Compounds 1, 2 and 4 showed moderate cytotoxic activity against Leukemia cells in vitro. Cytotoxic activity of compounds 1-4 showed $IC_{50}$ of 0.0097, 0.01, 0.097 and 0.0099 $\mu$ mol/mL respectively toward Leukemia cells and $IC_{50}$ of 0.097, 0.1, 0.097 and 0.099 $\mu$mol/mL toward Hela cells respectively. It is the first report that these compounds possess cytotoxic activity on Leukemia and Hela cells.
Chen, Peng,Wang, Xiu-Li,Ma, Zhong-Sen,Xu, Zhong,Jia, Bo,Ren, Jin,Hu, Yu-Xin,Zhang, Qing-Hua,Ma, Tian-Gang,Yan, Bing-Di,Yan, Qing-Zhu,Li, Yan-Lei,Li, Zhen,Yu, Jin-Yan,Gao, Rong,Fan, Na,Li, Bo,Yang, Jun Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7
HMGN5 is a typical member of the HMGN (high mobility group nucleosome-binding protein) family which may function as a nucleosomal binding and transcriptional activating protein. Overexpression of HMGN5 has been observed in several human tumors but its role in tumorigenesis has not been fully clarified. To investigate its significance for human lung cancer progression, we successfully constructed a shRNA expression lentiviral vector in which sense and antisense sequences targeting the human HMGN5 were linked with a 9-nucleotide loop. Inhibitory effects of siRNA on endogenous HMGN5 gene expression and protein synthesis were demonstrated via real-time RT-PCR and western blotting. We found HMGN5 silencing to significantly inhibit A549 and H1299 cell proliferation assessed by MTT, BrdU incorporation and colony formation assays. Furthermore, flow cytometry analysis showed that specific knockdown of HMGN5 slowed down the cell cycle at the G0/G1 phase and decreased the populations of A549 and H1299 cells at the S and G2/M phases. Taken together, these results suggest that HMGN5 is directly involved in regulation cell proliferation in A549 and H1299 cells by influencing signaling pathways involved in cell cycle progression. Thus, our finding suggests that targeting HMGN5 may be an effective strategy for human lung cancer treatment.
Net micromineral requirements for maintenance and growth of ewe lambs at the latter fattening period
Jin Ya Qian,Ding Na,Diao Xiao Gao,Yu Sheng Chen,Zhao Jun Xing,Zhang Jian Xin 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.9
Objective: The objective of this study was to investigate the net micromineral (Cu, Fe, Mn, Zn) requirements for maintenance and growth of Dorper×Jinzhong crossbred ewe lambs at their latter fattening period. Methods: Thirty 1/2 Dorper × 1/2 Jinzhong crossed F1 ewe lambs (35±0.5 kg of body weight [BW]) were used and divided into five groups in a randomized design for a comparative slaughter trial. At the beginning of the experiment, six lambs were randomly selected and slaughtered at 35 kg BW to determine their initial body composition. When their BWs reached 43 kg, another six lambs fed ad libitum were slaughtered to serve as an intermediate slaughter group. The retained eighteen lambs were randomly distributed into three groups and offered one type of feed at 100%, 65%, and 40% of the ad libitum intake. When the lambs fed ad libitum reached a BW of 50 kg, the three groups were slaughtered. The body composition (muscle, fat, bone, blood with viscera, skin, and wool) were weighted, ground, mixed, and subsampled for mineral content analysis. Results: The net maintenance requirements of Cu, Fe, Mn, and Zn were 0.017, 0.160, 0.004, and 0.067 mg/kg BW/d, respectively, and the net growth requirements per 100 grams of average daily gain ranged from 0.48 to 0.51 mg of Cu, 2.63 to 2.17 mg of Fe, 0.12 to 0.15 mg of Mn, and 2.07 to 2.00 mg of Zn, respectively, for Dorper × Jinzhong crossed ewes from 35 to 50 kg BW. Conclusion: Our results suggest that the micromineral requirements for both maintenance and growth of Dorper × Jinzhong crossbred ewe lambs were quite different from the recommendations of NRC (2007), except for Zn.