急性精神分裂障碍患者의 Haloperidol 治療時 臨床 好轉度와 血中 Prolactin 濃度의 關係

김성윤,주진형,우종인 大韓神經精神醫學會 1996 신경정신의학 Vol.35 No.2

저자들은 혈청 PRL농도가 급성 정신증에서의 haloperidol의 치료 반응에 대한 지표로서 임상적으로 이용가능한가를 규명하기 위하여 22명(남자 4, 여자 7)의 초발 정신분열양장애 환자들을 대상으로 하여 이 중 10명의 남자 환자군에 대해서는 11명의 남자 대조군과 기저 혈청 PRL치의 비교를 하였고 경구 haloperidol 치료를 받은 11명(남자 4, 여자 7)에 대해서는 치료전과 4주째의 혈청 PRL치와 haloperidol의 혈중 농도를 측정하고 동시에 Positive and Negative Syndrome Scale(PANSS)로 정신병리를 평가하였다. 결과는 다음과 같다. 1) 남자 환자군 10명의 치료전 혈청 PRL치는 정상 대조군의 혈청 PRL치와 유의한 차이를 보이지 않았다. 환자군에서 치료 개시전 PANSS로 측정한 정신상태 중 음성증상 subscale만이 혈청 PRL치와 유의한 상관관계(r=0.6636, p<0.05)를 보였다. 2) 4주간의 치료후 PANSS 점수 변화율은 기초 및 4주째의 혈청 PRL치와 유의한 상관관계를 보이지 않았고 PRL치의 변화율 및 변화치와도 유의한 상관관계를 보이지 않았다. 3) 4주간의 HALOPERIDOL 치료를 통해 PANSS 전체 점수는 유의한 감소를 보였으며(p<0.05), 양성증상(p<0.05)과 일반정신병리(p<0.05) subscale 점수상에서도 유의한 감소를 보였다. 혈청 PRL치도 기초 측정치에 비하여 4주째 혈청 PRL치, PRL치의 변화율 또는 변화치와 통계적으로 유의한 상관관계를 보이지 않았다. Objects : The authors investigated to evaluated the clinical utillity of serum prolactin(PRL) level as a therapeutic index of haloperidol treatment for acute psychotic patients. Methods : 22 patients(10 males, 12 females) first-onset schizophreniform disorder were studied. Among them 10 male patients' baseline PRL levels were compared to those of 11 agematched male volunteers and 11 patients who were treated with oral haloperidol(4 males, 7 females) were studied by measuring baseline and 4the week levels of serum PRL and haloperidol and administering Positive and Negative Syndrome Scale(PANSS) concurrently. Results : 1) The serum PRL level of 10 male patients showed no statistical difference from 11 healthy males. Only the patients' pre-treatment PANSS negative subscale score had significant correlation with the baseline serum PRL level. 2) Percentage of PANSS score change didn't show significant correlation with the baseline and 4th-week serum PRL level nor with amount and percentage of serum PRL level change. 3) The total PANSS score decreased significantly after 4 week treatment with haloperidol s well as positive symptoms subscale and general psychopathology(all, p<0.05), and 4th-week serum PRL level significantly increased(p<0.01) compared with the baseline. 4) The 4th-weeks plasma haloperidol level didn't show correlation with serum PRL level nor with amount and percentage of serum PRL level change. Conclusions : The findings suggest that serum PRL level has limitation for use as therapeutic index of haloperidol treatment for acute psychotic patients.

발효차중의 미량 성분인 gallic acid 산화물 purpurogallin carboxylic acid의 항염증 효과

주진우(Jin-Woo Jhoo) 한국식품과학회 2008 한국식품과학회지 Vol.40 No.6

본 연구의 목적은 발효차 중에 미량물질로 존재하는 purpurogallin 유도체의 항염증에 대한 효과를 검토하고자 실시하였다. 특히 발효차에 존재하는 미량 성분들에 대한 생리활성 연구보고는 많지 않은데 이러한 이유는 미량성분을 기술적으로 발효차로부터 분리 정제하기가 매우 어렵기 때문으로 판단된다. 특히 녹차잎에 존재하는 페놀 화합물중 하나인 gallic acid는 발효과정 중 benzotropolone 구조를 가지는 purpurogallin 유도체를 새롭게 만드는 것으로 알려져 있는데 본 연구에서는 gallic acid 화합물이 이러한 과정 중에 생성하는 산화물을 효소적 산화 모델시스템인 peroxidase/hydrogen peroxide 모델을 이용하여 산화물을 얻은 후 컬럼크로마토그라피법을 이용하여 순수 분리하였다. 이 과정에서 purpurogallin carboxylic acid(PCA)를 gallic acid의 산화물로 분리할 수 있었고 이의 구조를 ¹H NMR, ¹³C NMR 및 MS 분석방법을 이용하여 확인할 수 있었다. 이후 단리되어진 PCA의 항염증 효과를 검토하였다. RAW264.7 세포를 이용하여 PCA의 항염증 효과를 검토하였는데 PCA 100, 75, 50 ㎍/mL 처리는 NO의 생성을 LPS만 처리한 세포에 비해 각각 57.6, 41.5, 21.8%를 유의적으로 저해시키는 것으로 나타났다. PCA 100, 75 ㎍/mL 처리군에서 LPS만 처리한 RAW264.7 세포에 비해 IL-6의 생성이 각각 43.1, 23.9% 억제되어지는 것으로 관찰되었다. 또한 유사한 경향으로 PCA 100 및 75 ㎍/mL 처리군에서 LPS만 처리한 RAW264.7 세포에 비해 PGE₂의 생성이 각각 29.0% 및 15.4% 억제 되어지는 것으로 관찰되었다. 최근 생리활성을 가지는 신물질의 탐색 및 신소재 개발의 중요성이 부각되고 있다. 특히 선진국의 신물질의 개발에 의한 물질 특허가 증가하고 있고, 이에 따라 국내에서도 국제 경쟁력을 확보하기 위한 새로운 소재 개발이 시급한 상황이다. 본 연구 결과는 gallic acid의 산화물인 purpurogallin 유도체를 이용한 항염증 소재의 적용 가능성을 검토한 주요한 결과로 사료되어진다. 또한 본 연구를 통하여 발효차에 미량으로 존재하는 화합물들의 생리활성을 확인할 수 있었으며, 발효차에 포함되어 있는 미량 성분들의 항염증 관련 신소재 개발가능성을 검토할 수 있었다. The principal objective of the current study was to isolate a purpurogallin derivative as an oxidation product from gallic acid, in an effort to assess the anti-inflammatory effects of this compound. Purpurogallin derivative is known to be the one of the oxidation products of gallic acid. This compound has been identified as a minor chemical component in fermented tea products. It has been previously demonstrated that theaflavins, the oxidation products of catechins found in fermented tea products, exert profound antioxidant and anti-inflammatory effects. However, the biological activities of a minor chemical component in fermented teas have yet to be evaluated. Purpurogallin carboxylic acid (PCA) was identified as a major oxidation product of gallic acid from a peroxidase/hydrogen peroxide oxidation model system. The identity of the PCA was verified by ¹H NMR, ¹³C NMR and MS techniques. PCA treatment significantly suppressed the generation of pro-inflammatory mediators including nitric oxide and IL-6 in lipopolysaccharide (LPS)-stimulated RAW264.7 murine macrophages. According to the nitrite assay, PCA 100, 75, and 50 ㎍/mL treatment dose-dependently inhibited NO production by 57.6, 41.5, and 21.8%, respectively, in LPS-stimulated RAW264.7 murine macrophage cells. Moreover, IL-6 production was inhibited to a significant degree with PCA treatment of 100 and 75 ㎍/mL at 43.1 and 23.9%, respectively. PCA treatment also significantly suppressed PGE₂ production at levels of 100 and 75 ㎍/mL. These results showed that PCA exerts inhibitory effects on the production of inflammatory mediators.

No Evidence of Association of Interleukin 1A (-889) Genetic Polymorphism with Alzheimer's Disease in Koreans

Jhoo, Jin Hyeong,Park, Woong Yang,Kim, Ki Woong,Lee, Kwang Hyuk,Lee, Dong Young,Youn, Jong Chul,Suh, Young Ju,Seo, Jeong-Sun,Woo, Jong Inn Korea Genome Organization 2004 Genomics & informatics Vol.2 No.2

To examine whether the IL-1A (-889) polymorphism associates with a risk for Alzheimer's disease (AD) and acts interactively with the apolipoprotein (APOE) $\epsilon$4 in the development of AD, we performed genotype analyses of the IL-1A and the APOE of the 102 Korean AD patients and 200 Korean non-demented controls. We failed to detect a significant difference in genotypic and allelic frequencies of IL-1A between the AD group and control group. No overexpression of the IL-1A C/T genotype and IL-1A T allele was found when we analyzed the late-onset and early-onset patients, separately. There was no significant genetic interaction between IL-1A polymorphism and the APOE polymorphism. I n conclusion, the IL-1A polymorphism did not contribute to the development of AD independently or interactively with the APOE $\epsilon$4 allele in Koreans.

두 가지 한글판 간이정신상태평가(Mini-Mental State Examination)의 비교 : MMSE-KC와 K-MMSE

주진형,김기웅,이동영,윤종철,이태주,추일한,고혜정,서은현,우종인 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.1

Objectives : We compared the scores of the MMSE-KC (Korean version of MMSE in the Korean version of CERAD As-sessment Packet) and K-MMSE (Korean MMSE), and analyzed the influences of age, gender, and educational level on the differences between the two. Methods : We administered the MMSE-KC and K-MMSE simultaneously along with geriatric depression scale (GDS-K) to 733 community-dwelling elderly aged 50 or over in three districts of Suwon. The differences between the MMSE-KC scores and K-MMSE scores were examined by repeated measure ANOVA computing the MMSE for intra-individual factor, age, gender, and educational level as inter-individual factor, and GDS-K score as a covariate. Results : The scores of MMSE-KC and K-MMSE were identical only in the 17.8% of the subjects and the difference between the MMSE-KC score and K-MMSE score were significantly influenced by the educational level of the subjects (F (3,173) =8.21, p<0.001). The MMSE-KC score was higher than the K-MMSE score in illiterate subjects, and lower than the K-MMSE score in literate or educated subjects. Although the main effect of the kind of MMSE was not significant in total MMSE scores (F (1,731)=0.91, p>0.1), it was highly significant in 'judgement and writing/reading' subscores (F (1,731)=16.35, P<0.001) and its influences were significantly varied by the educational level of the subjects (F (1,731) =20.82, P<0.001). Conclusion : The MMSE-KC and K-MMSE which were standardized differently are not identical in scores as well as in content, and the difference between the two is greatly influenced by the educational level of the subject. When interpreting or comparing the MMSE scores of the two different versions of Korean MMSE, the comparability of the scores among them observed in this study should be considered.

알쯔하이머병의 말초 생물학적 지표로서 혈청 Alpha-1-antichymotrypsin

우종인,이정희,주진형 大韓神經精神醫學會 1996 신경정신의학 Vol.35 No.6

저자들은 대조군과 각 치매군의 혈청 alpha-1-antichymotrypsin(ACT) 수치를 측정하여 이것의 알쯔하이머병(AD)의 말초 지표로서의 가능성을 검토하고 혈청 ACT와 노화 과정사이의 상관성을 분석하고자 하였다. 치매의 진단을 위하여 NINCDS-ADRDA 기준을 이용하였고 혈청 ACT 수치에 영향을 미칠 수 있는 검사상의 이상자나 약물 복용자등은 연구에서 배제되었다. 최종 진단은 정신과 전문의 2명과 전공의 6명이 참여하여 합의 증례 토의를 통해서 내려졌다. 이러한 과정을 거쳐 10명의 probable AD 환자(나이 : 71.5±7.5, 범위 : 55∼82), 9명의 혈관 요인을 지닌 possible AD 환자(나이 : 72.3±8.2,범위 : 58∼89), 8명의 혈관성 치매(VD, 나이 : 72.5±9.4, 분포 : 56∼85)환자, 9명의 65세 이상의 정상 대조군(OC, 나이 : 70.9±4.6, 범위 : 66∼82저자들은 대조군과 각 치매군의 혈청 alpha-1-anti-chymotrypsin(ACT) 수치를 측정하여 ), 23명의 65세 미만의 정상 대조군(나이 : 31.2±13.0, 범위 : 21∼54)이 최종 대상군으로 설정되었다. 이들을 대상으로 72시간 단순 방사상 면역확산법을 시행하여 혈청 ACT 수치를 측정하였고 다음과 같은 결과를 얻었다. 1) Probable AD(420.0±75.6mg/L)군과 혈관 요인을 지니는 Possible AD군(443.7±54.6mg/L)의 평균 혈청 ACT 수치는 VD 환자군(483.3±89.4mg/L) 및 65세 이상의 정상대조군(OC, 382.4±20.0mg/L)과 비교하여 차이가 관찰되지 않았다. 2) VD군의 평균 혈청 ACT 수치는 OC군에 비해 의미있게 높았으며(p<0.05) 네 군을 혈관 요인이 존재하지 않는 OC과 probable AD 통합군과 혈관 요인이 존재하는 VD와 possible AD 통합군으로 재분류 하였을 때 재분류한 두 그룹사이의 평균 혈청 ACT 수치(402.1±58.3mg/L, 462.3±73.5mg/L)는 의미있는 차이를 나타냈다(p<0.05). 3) 정상 대조군의 혈청 ACT 수치는 연령이 높아짐에 따라 의미있게 증가하였다(r=0.5161;p<0.01)(Fig.3). 4) AD와 VD 환자의 clinical dementia rating, Blessed dementia scale 및 Short Blessed test 점수상의 치매의 정도와 혈청 ACT 수치 사이에는 상관 관계가 관찰되지 않았다. 이상의 결과는 혈청 ACT 수치의 증가가 치매 환자의 혈관 요인 및 정상적인 노화 과정과 연관되어 있음을 시사한다. 그러나 혈청 ACT 수치가 AD의 말초 지표일 가능성은 적을 것으로 판단된다. Objectives : The authors measured serum alpha-1-antichymotrypsin(ACT) level to elucidate its possibility to be a peripheral marker in Alzheimer's disease(AD) and to examine its relationship with aging. Methods : The subjects were 19 AD patients(age : 71.9±7.6, range : 55∼89) diagnosed by NINCDS-ADRDA criteria, 8 vascular dementia(VD) patients(age : 72.5±9.4, range : 56∼85), 32 normal control subjects(age : 42.4±21.4, range : 21∼82). Serum ACT levels were determined with radial immunodiffusion plates purchased from the Binding Site after 72-hour incubation. Results : The serum ACT levels of AD patients were not significantly different from those of old control(OC) subjects(age : 70.9±4.6, range : 66∼82), but ACT levels of VD patients were significantly higher than those of OC subjects(p<0.05). And there was significant correlation between ACT levels and age(r=0.5820, p<0.01). Conclusion : These results suggest that the increment of serum ACT level appears to be associated with the vascular component in dementia patients as well as with aging factor. However, its possibility to be a peripheral marker of AD seems to be low.

Antioxidant and hepatoprtective effects of eriodictyol against hydrogen peroxide induced oxidative stress in HepG2 cells

Tae-Woo Joo,Sun-Young Park,Jun-Hong Park,Gur-Yoo Kim,Jin-Woo Jhoo 한국식품영양과학회 2015 한국식품영양과학회 학술대회발표집 Vol.2015 No.8

Qualitative Characteristics and Determining Shelf- Life of Milk Beverage Product Supplemented with Coffee Extracts

Ji-woo Yoon,Sung-il Ahn,Ha-na Kim,Jun-hong Park,Sun-young Park,Jae-hoon Kim,Duk-geun Oh,Jin-woo Jhoo,Gur-yoo Kim 한국축산식품학회 2017 한국축산식품학회지 Vol.37 No.2

This study was conducted to establish the shelf-life of a milk beverage product supplemented with coffee extracts. Qualitative changes including peroxide value (PV), microorganism content, caffeine content, and sensory evaluation were measured periodically in beverages kept at 10, 20, and 30℃ for 8 wk. Lipid oxidation of the product was measured by peroxide value analysis, and apparent changes were observed during a 4 wk storage period. Caffeine analysis revealed that the changes in caffeine content were negligible during the storage period. Total aerobic bacteria, Escherichia coli, yeast, and mold were not detected in the products during an 8 wk storage period. Sensory evaluation revealed that after 4 wk of storage overall acceptance was less than 3 points on a 5-point scale. In this study, PV was used as an indicator of the shelflife of the milk beverage product. PV analysis revealed that a value of 20 meq/kg was the end of the shelf-life using the Arrhenius equation and the accelerated shelf-life test (ASLT). Assuming that the beverages are kept at 4℃ during distribution, calculation of when the PV reached the quality limit point (20 meq/kg) was done with the equation ln(PV) = 0.3644X - 2.21834 and, using that equation, PV = e0.3644X-2.21834 was calculated. Therefore, 14.3086 wk was determined to be the shelf-life of the milk beverage supplemented with coffee when stored at 4℃.

Antioxidant Activity of Yogurt Fermented at Low Temperature and Its Anti-inflammatory Effect on DSS-induced Colitis in Mice

Ji-Woo Yoon,Sung-Il Ahn,Jin-Woo Jhoo,Gur-Yoo Kim 한국축산식품학회 2019 한국축산식품학회지 Vol.39 No.1

This study was performed to evaluate the antioxidant activity of yogurt fermented at low temperature and the anti-inflammatory effect it has on induced colitis with 2.5% dextran sodium sulfate (DSS) in Balb/c mice. Yogurt premix were fermented with a commercial starter culture containing Lactobacillus acidophilus, Bifidobacterium lactis, Streptococcus thermophilus, and Lactobacillus delbrueckii subsp. bulgaricus at different temperatures: 22℃ (low fermentation temperature) for 27 h and 37℃ (general fermentation temperature) for 12 h. To measure antioxidant activity of yogurt samples, DPPH, ABTS+ and ferric reducing antioxidant potential (FRAP) assays were conducted. For animal experiments, inflammation was induced with 2.5% DSS in Balb/c mice. Yogurt fermented at low temperature showed higher antioxidant activity than that of the yogurt fermented at general temperature. In the inflammatory study, IL-6 (interleukin 6) was decreased and IL-4 and IL-10 increased significantly in DSS group with yogurt fermented at general temperature (DYG) and that with yogurt fermented at low temperature (DYL) compared to that in DSS-induced colitic mice (DC), especially DYL had higher concentration of cytokines IL-4, and IL-10 than DYG. MPO (myeloperoxidase) tended to decrease more in treatments with yogurt than DC. Additionally, yogurt fermented at low temperature had anti-inflammatory activity, although there was no significant difference with general temperature-fermented yogurt (p>0.05).

Effect of Trehalose on Lactobacillus Viability and Antioxidant activity of Low Temperature Fermented Frozen Yogurt

Ji-Woo Yoon,Sun-Young Park,Jun-Hong Park,Ha-Na Kim,Sung-Il Ahn,Jae-Hoon Kim,Duk-Geun Oh,Jin-Woo Jhoo,Gur-Yoo Kim 한국식품영양과학회 2016 한국식품영양과학회 학술대회발표집 Vol.2016 No.10

The Effect of Trehalose for Lactobacillus Survival during Low Temperature Fermentation in Yogurts and Its Antioxidant Activity

Ji-Woo Yoon,Ha-Na Kim,Jin-Woo Jhoo,Gur-Yoo Kim 한국식품영양과학회 2017 한국식품영양과학회 학술대회발표집 Vol.2016 No.10