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Thiobacillus sp. IW.에 의한 황화수소 제거
차진명,고한철,이인화 조선대학교 부설 유전생물공학연구소 1994 유전생물공학연구지 Vol.4 No.-
Thiobacillus sp. IW., capable of oxidizing hydrogen sulfide, was isolated from waste coal mine water around Hawsun in Chunnam province. The isolate was motile gram-negative rod shape, formed spore and grew up to be aerobically facultative chemolithotroph by using energy released from the oxidation of reduced inorganic sulfur compounds. It was found that the optimum thiosulfate concentration for growth was 32mM. The G+C content of the DNA was 65.0 mol%. The isolate had 16:1+17cyc, 16:0 as their major non-hydroxylated cellular fatty acids, 3-OH 12:0 as a hydroxylated fatty acid and also contained unidentified C_18 branched fatty acid. The ubiquinone system in the respiratory chain was Q-9. The cell productivity was 6.25mg/1.h and the isolate grew logarithmically up to 18 hour. The maximum specific growth rate of cell was 0.78h^-1 and generation time was 0.9 hour. In order to oxidize hydrogen sulfide in a bubble column reactor, Thiobacillus sp. IW. was immobilized on Polyvinylpyridine(PVP). 1.2l of broth medium containing 7.5g of immobilized PVP was used as a liquid phase and gas was supplied through the sintered glass distributor under hydrogen sulfide concentration controlled air stream. A Michaelis-Menten equation was applied for kinetics modeling and the maximum removal rate(V_m) and saturation constant(K_s) of hydrogen sulfide were 52.4(g-S/kg-dry PVP.d) and 62.88ppm, respectively.
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노성희,차진명,김선일 한국공업화학회 2005 응용화학 Vol.9 No.1
In this study, we developed impregnated activated carbon manufacturing process, and investigated the possibility of using coconut as a activated carbon for odor removal. Among the five collectors, KCI, NaOH, Ki, HNO₃, and H₂SO₄, that were added to the adsorption process for the odor removal of H₂S and NH₃, the treatment with H₂SO₄ showed the best result having the adsorption quantity of 4.6 mg/g.
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홍주현,진계환,박경순,조명찬,박길선,차은종,이태수 충북대학교 의과대학 충북대학교 의학연구소 2003 忠北醫大學術誌 Vol.13 No.2
연구목적: 초소형 정밀 기계 기술로 제작된 2차원 가속도 센서와 916 MH2 단거리 고주파 데이터 통신 기술을 응용하여 인체의 운동상태를 자동으로 감지하고 분류하는 기법을 제안 하고자 하였다. 재료 및 방법: 인체의 신호를 획득하기 위하여 미국 버클리대에서 제작한 무선 센서를 등 산용 조끼의 전면상단에 부착해서, 대상자의 흉골 부위에 위치하도록 하고, 앉은 상태에서 일어서거나 눕거나 하는 동작 및 그 역동작이 일어날 때, 가속도 센서에서 감지되는 4가지 종류의 파형 현화에 해당되는 데이터를 무선센서 네트웍을 통해 획득하였다. 결과: 획득한 신호를 주성분분석(Principal Component Analysis)과 지원벡터머신(Support Vector Machine)을 사용하여, 2차주성분까지를 도표화하고 클러스터링 함으로써, 4개의 영역으로 분할하는 것이 가능함을 말 수 있었다 결론: 소형 센서들이 작동되는 임베디드 컴퓨팅 환경에서 일상생활 중에 발생하는 인체의 4가지 동작을 자동으로 감지하고 분류하는 것이 가능함을 보였다. Purpose: New monitoring and classifying method of human motion contort was pro- posed by using 2-axial MEMS accelerometer and 916 MHz short-range data communica-tion technology. Materials and Methods: The wireless sensor of MICA mote was attached to a woman mountain vest and located close to the subject's sternum. During four types of sub-ject's motion, that is, sit-to-stand, stand-to-sit, sit-to-tying, and tying-to-sit, waveform changes of the accelerometer data were acquired by wireless sensor network. Results: The acquired signal was analyzed by Principal Component Analysis(PCA) and Support Vector Machine(SVM) technique. By clustering the first and second principal components, four regions corresponding to the four types of motion, were clearly segment-ed. Conclusion: Human motion context during daily life could be monitored and classified automatically by using small sensors in embedded computing environment
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Optimal Conditions for Chitinase Production by Serratia marcescens
Cha, Jin-Myeong,Cheong, Kyung-Hoon,Cha, Wol-Suk,Choi, Du-Bok,Roh, Sung-Hee,Kim, Sun-Il The Korean Society for Biotechnology and Bioengine 2004 Biotechnology and Bioprocess Engineering Vol.9 No.4
A chitinase-producing bacterium was isolated from seashore mud around Beobseongpo in Chunmam province through the use of a selective enrichment culture. The best chitinase producing strain was isolated and identified as Serratia marcescens KY from its characteristics. For effective production of chitinase, optimum pH, temperature, and agitation speed were investigated in flask cultures. The optimum pH using Serratia marcescens KY was between pH 6 and 7 and the chitinase produced was 37.9 unit/mL. On the other hand, the optimal pH of the Serratia marcescens ATCC 27117 was 7.5, and the produced amount of chitinase was 35.2 unit/mL. The optimal temperature for chitinase production for Serratia marcescens KY and Serratia marcescens ATCC 27117 was $30^{\circ}$. The cell growth pattern at different temperature was almost identical to the chitinase production. To investigate the optimal shaking speed under optimal culture, speeds were varied in the range of 0∼300 rpm. The maximum production of chitinase was carried at 200 rpm although the cell growth was the highest at 150 rpm. It indicates that oxygen adjustment is required for the maximum chitinase production. Using optimal conditions, batch cultures for comparing Serratia marcescens KY and Serratia marcescens ATCC 27117 were carried out in a 5 L fermentor. The oxygen consumption was increased with the increase of culture. Especially, at 120 h of culture Serratia marcescens KY and Serratia marcescens ATCC 27117 produced 38.3 unit/mL, and 33.5 unit/mL, respectively.
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미혼모와 대학생의 가족기능, 자아분화 및 생활자세에 관한 연구
차명진 ( Myeong Jin Cha ),제석봉 ( Suk Bong Je ) 한국가족사회복지학회 2010 한국가족복지학 Vol.29 No.-
본 연구는 미혼모와 대학생의 생활자세, 자아분화, 가족기능의 비교분석과 함께 세 변인간의 관계를 봄으로써 미혼모의 특성을 파악하고자 하였다. 이를 위해 전국의 미혼모 생활시설의 미혼모와 대학생을 대상으로 설문조사하였다. 분석결과 미혼모와 대학생의 생활자세는 타인부정을 제외한 3개 영역, 즉 자기긍정, 자기부정, 타인긍정에서 유의적인 차이가 있는 것으로 분석되었으며, 대학생에 비하여 자기긍정과 타인긍정이 낮고 자기부정과 타인부정이 높은 것으로 조사됨으로써 미혼모의 생활자세가 대학생에 비하여 부정적이라는 사실이 확인되었다. 자아분화의 5개 하위 영역과 가족기능의 6개 하위 영역 중 대학생과 미혼모의 생활자세에 영향력 있는 예측변수의 파악을 위해 단계적 회귀분석을 실시한 결과, 대학생의 경우 네 가지 생활자세에 영향을 미치는 공통변수는 인지대정서기능과 커뮤니케이션인 것으로 나타났다. 미혼모의 경우 자기긍정과 타인긍정의 생활자세에는 대학생과 동일한 인지대정서기능과 커뮤니케이션 이외에 문제해결이, 자기부정과 타인부정의 생활자세에는 정서적 반응과 정서적 단절인 것으로 나타났다. 이러한 결과는 삶의 태도를 결정하는 생활자세가 대학생과 미혼모간 차이가 있음은 물론 생활자세 형성과 관련있는 변수들에 차이가 있음을 확인함으로써 미혼모를 대상으로 한 상담이나 교육현장에서 이들의 특성을 고려한 접근방법들이 모색되고 제시되어야 함을 시사한다. This study identified family function, self differentiation, and life position; it also attempted to identify variables that influence life position. In addition, this study explored the differences between the college students and unmarried mothers. A self-administered questionnaire was designed and administered to college students living in Daegu and unmarried mothers who live in institutional care centers. A total of 268 questionnaires(110 college students, 158 unmarried mothers) were collected for a response rate of 89.33%. The results of t-test comparing four types of life position between college students and unmarried mothers showed that I`m OK. I`m not OK and You`re OK were significantly different. Especially, for unmarried mothers, I`m not OK and You`re not OK were much greater among unmarried mothers than among college students, identifying unmarried mothers` negative life position. Stepwise regression analysis was performed to examine the influence of self differentiation and family function; we analyzed 5 subcategories of self differentiation and 6 subcategories of family function on life position. There were totally different between college students and unmarried mothers in variables affecting life position. College students` life position on I`m OK was affected by communication, intelligence to emotional function, self integration. For unmarried mothers, problem solving, family regression, communication, and intelligence to emotional function influenced on life position of I`m OK. Family regression influenced life position of I`m not OK among both college students and unmarried mothers survey respondents differently. problem solving influenced only unmarried mothers` life position of You`re OK. From a practical viewpoint, effective counseling and educational programs, which consider different unmarried mothers characteristics with college students, should carefully integrate to control the positive family function and self differentiation for improving unmarried mothers` life position.
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영산강 수계의 비점오염원에 관한 연구1 - 토지이용 및 강우를 중심으로 -
차진명(Jin Myeong Cha),신성의(Sung Euy Shin),차규석(Gyu Suk Cha) 한국환경영향평가학회 1999 환경영향평가 Vol.8 No.1
This study was carried out to estimate the runoff loading characteristics of the non-point source pollutions in the Youngsan river basins by the method of land-use types and rainfall. The lysimeter test, rainfall and stream flowmeter measurement were performed to develop the pollutant loading unit discharged from the non-point sources. As the non-point sources, the unit pollutant discharge rates were different from the land-use types such as paddy field, upland, forest, housing site and others. The pollutant loading units classified by land-use types in the Youngsan river basins are as follows : The total BOD loading rate is 15.3 ton/day and the housing site is discharged 50.6%, the total T-N loading rate is 6.0 ton/day and the paddy field and upland is discharged 77.6%, and the total T-P loading rate is 0.39 ton/day and the paddy field and upland is discharged 81.2%. The pollutant loadings by rainfall in the Youngsan river basins are about 7,425 ton/year of BOD, 324 ton/year of T-N and 118 ton/year of T-P, respectively.
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