Thiobacillus sp. IW.에 의한 황화수소 제거

차진명,고한철,이인화 조선대학교 부설 유전생물공학연구소 1994 유전생물공학연구지 Vol.4 No.-

Thiobacillus sp. IW., capable of oxidizing hydrogen sulfide, was isolated from waste coal mine water around Hawsun in Chunnam province. The isolate was motile gram-negative rod shape, formed spore and grew up to be aerobically facultative chemolithotroph by using energy released from the oxidation of reduced inorganic sulfur compounds. It was found that the optimum thiosulfate concentration for growth was 32mM. The G+C content of the DNA was 65.0 mol%. The isolate had 16:1+17cyc, 16:0 as their major non-hydroxylated cellular fatty acids, 3-OH 12:0 as a hydroxylated fatty acid and also contained unidentified C_18 branched fatty acid. The ubiquinone system in the respiratory chain was Q-9. The cell productivity was 6.25mg/1.h and the isolate grew logarithmically up to 18 hour. The maximum specific growth rate of cell was 0.78h^-1 and generation time was 0.9 hour. In order to oxidize hydrogen sulfide in a bubble column reactor, Thiobacillus sp. IW. was immobilized on Polyvinylpyridine(PVP). 1.2l of broth medium containing 7.5g of immobilized PVP was used as a liquid phase and gas was supplied through the sintered glass distributor under hydrogen sulfide concentration controlled air stream. A Michaelis-Menten equation was applied for kinetics modeling and the maximum removal rate(V_m) and saturation constant(K_s) of hydrogen sulfide were 52.4(g-S/kg-dry PVP.d) and 62.88ppm, respectively.

새마을기계화영농단의 적정모델설정에 관한 연구

김철기, 차균도, 유진채, 박명근 忠北大學校 새마을硏究所 1985 새마을 硏究 論文集 Vol.4 No.-

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냄새제거용 첨착활성탄 개발에 관한 연구

노성희,차진명,김선일 한국공업화학회 2005 응용화학 Vol.9 No.1

In this study, we developed impregnated activated carbon manufacturing process, and investigated the possibility of using coconut as a activated carbon for odor removal. Among the five collectors, KCI, NaOH, Ki, HNO₃, and H₂SO₄, that were added to the adsorption process for the odor removal of H₂S and NH₃, the treatment with H₂SO₄ showed the best result having the adsorption quantity of 4.6 mg/g.

이동형 생체계측을 위한 임베디드 컴퓨팅

홍주현,진계환,박경순,조명찬,박길선,차은종,이태수 충북대학교 의과대학 충북대학교 의학연구소 2003 忠北醫大學術誌 Vol.13 No.2

연구목적: 초소형 정밀 기계 기술로 제작된 2차원 가속도 센서와 916 MH2 단거리 고주파 데이터 통신 기술을 응용하여 인체의 운동상태를 자동으로 감지하고 분류하는 기법을 제안 하고자 하였다. 재료 및 방법: 인체의 신호를 획득하기 위하여 미국 버클리대에서 제작한 무선 센서를 등 산용 조끼의 전면상단에 부착해서, 대상자의 흉골 부위에 위치하도록 하고, 앉은 상태에서 일어서거나 눕거나 하는 동작 및 그 역동작이 일어날 때, 가속도 센서에서 감지되는 4가지 종류의 파형 현화에 해당되는 데이터를 무선센서 네트웍을 통해 획득하였다. 결과: 획득한 신호를 주성분분석(Principal Component Analysis)과 지원벡터머신(Support Vector Machine)을 사용하여, 2차주성분까지를 도표화하고 클러스터링 함으로써, 4개의 영역으로 분할하는 것이 가능함을 말 수 있었다 결론: 소형 센서들이 작동되는 임베디드 컴퓨팅 환경에서 일상생활 중에 발생하는 인체의 4가지 동작을 자동으로 감지하고 분류하는 것이 가능함을 보였다. Purpose: New monitoring and classifying method of human motion contort was pro- posed by using 2-axial MEMS accelerometer and 916 MHz short-range data communica-tion technology. Materials and Methods: The wireless sensor of MICA mote was attached to a woman mountain vest and located close to the subject's sternum. During four types of sub-ject's motion, that is, sit-to-stand, stand-to-sit, sit-to-tying, and tying-to-sit, waveform changes of the accelerometer data were acquired by wireless sensor network. Results: The acquired signal was analyzed by Principal Component Analysis(PCA) and Support Vector Machine(SVM) technique. By clustering the first and second principal components, four regions corresponding to the four types of motion, were clearly segment-ed. Conclusion: Human motion context during daily life could be monitored and classified automatically by using small sensors in embedded computing environment

Optimal Conditions for Chitinase Production by Serratia marcescens

Cha, Jin-Myeong,Cheong, Kyung-Hoon,Cha, Wol-Suk,Choi, Du-Bok,Roh, Sung-Hee,Kim, Sun-Il The Korean Society for Biotechnology and Bioengine 2004 Biotechnology and Bioprocess Engineering Vol.9 No.4

A chitinase-producing bacterium was isolated from seashore mud around Beobseongpo in Chunmam province through the use of a selective enrichment culture. The best chitinase producing strain was isolated and identified as Serratia marcescens KY from its characteristics. For effective production of chitinase, optimum pH, temperature, and agitation speed were investigated in flask cultures. The optimum pH using Serratia marcescens KY was between pH 6 and 7 and the chitinase produced was 37.9 unit/mL. On the other hand, the optimal pH of the Serratia marcescens ATCC 27117 was 7.5, and the produced amount of chitinase was 35.2 unit/mL. The optimal temperature for chitinase production for Serratia marcescens KY and Serratia marcescens ATCC 27117 was $30^{\circ}$. The cell growth pattern at different temperature was almost identical to the chitinase production. To investigate the optimal shaking speed under optimal culture, speeds were varied in the range of 0∼300 rpm. The maximum production of chitinase was carried at 200 rpm although the cell growth was the highest at 150 rpm. It indicates that oxygen adjustment is required for the maximum chitinase production. Using optimal conditions, batch cultures for comparing Serratia marcescens KY and Serratia marcescens ATCC 27117 were carried out in a 5 L fermentor. The oxygen consumption was increased with the increase of culture. Especially, at 120 h of culture Serratia marcescens KY and Serratia marcescens ATCC 27117 produced 38.3 unit/mL, and 33.5 unit/mL, respectively.

Chitinase 생성을 위한 배양 조건 최적화

차진명(Jin Myeong Cha),석근영(Keun Young Suk),차월석(Wol Suk Cha) 한국생물공학회 2001 KSBB Journal Vol.16 No.4

기포탑 반응기상 (床) 에서 Thiobacillus sp . IW . 고정화 Polyvinylpyridine 를 이용한 황화수소 제거

차진명(Jin Myeong Cha),이인화(In Wha Lee) 大韓環境工學會 1995 대한환경공학회지 Vol.17 No.2

영산강 수계의 비점오염원에 관한 연구1 - 토지이용 및 강우를 중심으로 -

차진명(Jin Myeong Cha),신성의(Sung Euy Shin),차규석(Gyu Suk Cha) 한국환경영향평가학회 1999 환경영향평가 Vol.8 No.1

This study was carried out to estimate the runoff loading characteristics of the non-point source pollutions in the Youngsan river basins by the method of land-use types and rainfall. The lysimeter test, rainfall and stream flowmeter measurement were performed to develop the pollutant loading unit discharged from the non-point sources. As the non-point sources, the unit pollutant discharge rates were different from the land-use types such as paddy field, upland, forest, housing site and others. The pollutant loading units classified by land-use types in the Youngsan river basins are as follows : The total BOD loading rate is 15.3 ton/day and the housing site is discharged 50.6%, the total T-N loading rate is 6.0 ton/day and the paddy field and upland is discharged 77.6%, and the total T-P loading rate is 0.39 ton/day and the paddy field and upland is discharged 81.2%. The pollutant loadings by rainfall in the Youngsan river basins are about 7,425 ton/year of BOD, 324 ton/year of T-N and 118 ton/year of T-P, respectively.

폐탄광수에서 분리한 황화수소 분해 세균 Thiobacillus sp. IW.의 성장 조건

차진명(Jin-Myeong Cha),박열(Yeol Park),이인화(In-Wha Lee) 한국생물공학회 1994 KSBB Journal Vol.9 No.3

액상촉매를 이용한 SO₂/NO 동시제거 공정의 최적화 연구

배진열(Jin Youl Bae),정승호(Seung Ho Jung),박돈희(Don Hee Park),박상일(Sang Il Park),정경훈(Kyung Hoon Jung),차규석(Kyu Suk Cha),장태사(Tae Sa Jang),차진명(Jin Myeong Cha) 大韓環境工學會 2007 대한환경공학회지 Vol.29 No.10

화석연료의 사용 증가로 인해 대기오염이 전지구적 차원에서 심각한 문제가 되고 있다. 특히 화석연료의 연소 시 발생되는 SOx, NOx는 다양한 문제를 발생시키기 때문에 발생을 저감시키는 것이 대기오염 관리에서 중요한 목적이 되고 있다. SOx, NOx를 제거하기 위한 다양한 공정이 개발되었으나 몇 가지 문제점들을 가지고 있다. 본 연구의 목적은 연소가스 중에 포함되어 있는 SO₂/NO를 동시제거하기 위해 최적의 액상촉매 조성물, 농도 및 반응조건을 도출하는 데 있다. 다양한 금속염과 킬레이트 및 실험조건에 따른 가스의 제거효율을 살펴보았으며. 그 결과 철염과 EDTA를 선정할 수 있었으며 적정 농도는 각각 0.03 M이었다. 최적반응조건은 pH=8, Ca/S 당량비=1.5이었으며 O₂ 가스 농도는 낮을수록 유리하였다. 본 연구조건 하에서 촉매 성능을 증가시키기 위한 안정제 조성물과 농도는 ascorbic acid=0.024 M, adipic acid=0.024 M, sodium sulfite=0.09 M로 결정되었다. 다만 안정제의 농도가 본 연구의 조건보다 증가한다면 가스의 제거효율도 증가할 것으로 예상된다. With increasing use of fossil fuel, air pollution is becoming a serious problem in the world. Especially SOx and NOx, which are formed when fossil fuel burns, generate various problem and thus abatement of their emission is a major target in air pollution control. Various process have developed to remove SOx and NOx, but that has several disadvantage. This study aimed at selecting the optimum liquid catalyst component, concentration and reaction conditions for simultaneous removal of SO₂/NO in flue gas. It was that make on investigation into SO₂/NO simultaneous removal efficiencies using some metallic salts and chelates under various conditions, which optimum components were selected iron salts and EDTA and its concentrations were 0.03 M respectively. The optimum reaction conditions were pH=8.0, Ca/S equivalent ratio=1.5 and low O₂ concentration. Under the testing conditions of this study, additive component and concentration to increase performance of liquid catalyst were as follow: ascorbic acid=0.024 M, adipic acid=0.024 M and sodium sulfite=0.09 M. But SO₂/NO simultaneous removal efficiency would be increased if additive concentration was to increase in liquid catalyst.