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Kim, S-H,Kim, E-J,Hitomi, M,Oh, S-Y,Jin, X,Jeon, H-M,Beck, S,Jin, X,Kim, J-K,Park, C G,Chang, S-Y,Yin, J,Kim, T,Jeon, Y-j,Song, J,Lim, Y C,Lathia, J D,Nakano, I,Kim, H Macmillan Publishers Limited 2015 CELL DEATH AND DIFFERENTIATION Vol.22 No.9
Glioblastomas (GBMs) maintain their cellular heterogeneity with glioma stem cells (GSCs) producing a variety of tumor cell types. Here we interrogated the oncogenic roles of Lim domain only 2 (LMO2) in GBM and GSCs in mice and human. High expression of LMO2 was found in human patient-derived GSCs compared with the differentiated progeny cells. LMO2 is required for GSC proliferation both in vitro and in vivo, as shRNA-mediated LMO2 silencing attenuated tumor growth derived from human GSCs. Further, LMO2 is sufficient to induce stem cell characteristics (stemness) in mouse premalignant astrocytes, as forced LMO2 expression facilitated in vitro and in vivo growth of astrocytes derived from Ink4a/Arf null mice and acquisition of GSC phenotypes. A subset of mouse and human GSCs converted into vascular endothelial-like tumor cells both in vitro and in vivo, which phenotype was attenuated by LMO2 silencing and promoted by LMO2 overexpression. Mechanistically, the action of LMO2 for induction of glioma stemness is mediated by transcriptional regulation of Jagged1 resulting in activation of the Notch pathway, whereas LMO2 directly occupies the promoter regions of the VE-cadherin gene for a gain of endothelial cellular phenotype. Subsequently, selective ablation of human GSC-derived VE-cadherin-expressing cells attenuated vascular formation in mouse intracranial tumors, thereby significantly prolonging mouse survival. Clinically, LMO2 expression was elevated in GBM tissues and inversely correlated with prognosis of GBM patients. Taken together, our findings describe novel dual roles of LMO2 to induce tumorigenesis and angiogenesis, and provide potential therapeutic targets in GBMs.
이성교제 중인 대학생의 사이버섹스 중독, 외로움 및 이성관계 만족에 관한 연구
김윤정,박지야,변수영,이겨레,이아름,이지영,임재희,조혜림,진진화,최재의,박효정,이혜림 이화여자대학교 간호과학대학 2016 이화간호학회지 Vol.- No.50
Objectives: The purpose of the study was to identify the relationships between cybersex addition, loneliness and relationship satisfaction among Korean college students. Methods: 293 students were conveniently selected. The instruments used in this study were cyber-sexual addiction index, loneliness scale and relationship assesment scale. ANOVA, t-test and Pearson correlation coefficient with SPSS 22.0 Version were used to analyze the data. Results: 8.9% of the participants had moderate or severe addiction to cybersex. The level of cybersex addiction is significantly influenced by gender, period of relationship, time taking to sexual relations, contact number of cyber pornography and contact time of cyber pornography. Significant association was observed between cybersex addition, loneliness and relationship satisfaction. Conclusions: The factors mentioned in this study as well as loneliness and relationship satisfaction were important factors. Therefore, future studies on this topics need to develope interventions to reflect cultural and physical character of college students.
임정호,류관희,진제용 한국농업기계학회 2003 바이오시스템공학 Vol.28 No.1
this study was carried out to design, construct, and test a greenhouse monitoring system for the environment and status of control devices in a greenhouse from a remote site using internet. The measuring items selected out of many environmental factors were temperature, humidity, solar radiation, CO_2, SO_x, NO_x concentration, EC, pH of nutrient solution, the state of control devices, and the image of greenhouse. The network system consists of the three kinds of monitors named the Group Monitor and the Server Monitor and the Server Monitor. The results of the study are summarized as follows. 1.The measuring module named the House Monitor, which is used to watch the state of the control device and the environment of the greenhouse, was developed to a embedded monitoring module using one chip microprocessor. 2.For all measuring items, the House Monitor showed a satisfactory accuracy within the range of ±0.3%FS. The House Monitors were connected to the Group Monitor by communication method of RS-485 type and could operate under power and communication fault condition within 10 hours. The Group Monitor was developed to receive and display measurement data received from the House and to control the greenhouse environmental devices. 3.The image of the plants inside greenhouse were captured by PC camera and sent to the Group Monitor. the greenhouse manager was able to monitor the growth state of plants inside greenhouse without visiting individual greenhouse. 4.Remote monitoring the greenhouse environment and status of control devices was implemented in a client/server environment. The client monitor of the greenhouse manager at a remote site or other greenhouse manager was able to monitor the greenhouse environment and the state of control devices from the Server Monitor using internet.
Byun, Hae-Ok,Kim, Hee Young,Lim, Jin J.,Seo, Yong-Hak,Yoon, Gyesoon Wiley Subscription Services, Inc., A Wiley Company 2008 Journal of cellular biochemistry Vol.104 No.5
<P>Mitochondrial complex II defect has recently been implicated in cellular senescence and in the ageing process of which a critical phenotype is retardation and arrest of cellular growth. However, the underlying mechanisms of how complex II defect affects cellular growth, remain unclear. In this study, we investigated the effect of complex II inhibition using a subcytotoxic dose (400 µM) of 2-thenoyltrifluoroacetone (TTFA), a conventional complex II inhibitor, on cell cycle progression. TTFA (400 µM) directly decreased KCN-sensitive cellular respiration rate to 67% of control and disrupted the mitochondrial membrane potential. In contrast to other respiratory inhibitors such as rotenone, antimycin A, and oligomycin, TTFA prolonged the duration of each phase of the cell cycle (G1, S, and G2/M) equally, thereby delaying overall cell cycle progression. This delay was accompanied by a biphasic increase of reactive oxygen species (ROS) and concurrent glutathione oxidation, in addition to a slight decrease in the cellular ATP level. Finally, the delay in cell cycle progression caused by TTFA was proved to be mainly due to ROS overproduction and subsequent oxidative stress, as evidenced by its reversal following pretreatment with antioxidants. Taken together, these results suggest that an overall delay in cell cycle progression due to complex II defects may contribute to ageing and degenerative diseases via inhibition of cellular growth and proliferation without arrest at any specific phase of the cell cycle. J. Cell. Biochem. 104: 1747–1759, 2008. © 2008 Wiley-Liss, Inc.</P>
Han, Min-Su,Kim, Hyo-Jin,Wee, Hee-Jun,Lim, Kyung-Eun,Park, Na-Rae,Bae, Suk-Chul,van Wijnen, Andre J.,Stein, Janet L.,Lian, Jane B.,Stein, Gary S.,Choi, Je-Yong Wiley Subscription Services, Inc., A Wiley Company 2010 Journal of cellular biochemistry Vol.110 No.1
<P>Cleidocranial dysplasia (CCD) is caused by haploinsufficiency in RUNX2 function. We have previously identified a series of RUNX2 mutations in Korean CCD patients, including a novel R131G missense mutation in the Runt-homology domain. Here, we examine the functional consequences of the RUNX2<SUP>R131G</SUP> mutation, which could potentially affect DNA binding, nuclear localization signal, and/or heterodimerization with core-binding factor-β (CBF-β). Immunofluorescence microscopy and western blot analysis with subcellular fractions show that RUNX2<SUP>R131G</SUP> is localized in the nucleus. Immunoprecipitation analysis reveals that heterodimerization with CBF-β is retained. However, precipitation assays with biotinylated oligonucleotides and reporter gene assays with RUNX2 responsive promoters together reveal that DNA-binding activity and consequently the transactivation of potential of RUNX2<SUP>R131G</SUP> is abrogated. We conclude that loss of DNA binding, but not nuclear localization or CBF-β heterodimerization, causes RUNX2 haploinsufficiency in patients with the RUNX2<SUP>R131G</SUP> mutation. Retention of specific functions including nuclear localization and binding to CBF-β of the RUNX2<SUP>R131G</SUP> mutation may render the mutant protein an effective competitor that interferes with wild-type function. J. Cell. Biochem. 110: 97–103, 2010. © 2010 Wiley-Liss, Inc.</P>
열선 화학 기상 증착법에 의한 실리콘 박막 및 태양전지 특성
김상균(Kim, Sang-Kyun),이정철(Lee, Jeong Chul),전상원(Jeon, Sang Won),임충현(Lim, Chung Hyun),안세진(Ahn, Sae Jin),윤재호(Yun, Jae Ho),김석기(Kim, Seok Ki),송진수(Song, Jinsoo),박성주(Park, S-J),윤경훈(Yoon, Kyung Hoon) 한국신재생에너지학회 2005 한국신재생에너지학회 학술대회논문집 Vol.2005 No.06
최근 열선 화학 기상 증착법(HWCVD)은 낮은 온도에서 TFT용 Poly Si 중착을 할 수 있다는 점과 실리콘 박막을 빠른 속도로 증착할 수 있다는 점에서 각광을 받고 있다. 본 연구에서는 HWCVD를 이용하여 태양전지를 제조하고 그 특성을 평가하였다. 조건에 따른 실리콘 박막의 특성 변화를 알기 위해 corning glass 및 실리콘 wafer에 다양한 조건에서 단위 박막(intrinsic layer)을 증착하였고 이 결과를 바탕으로 p/i/n 구조의 태양전지를 제조하였다. Ta 열선 온도는 1700-2000도였고 가스 원료인 SiH₄와 수소의 비율을 조절하면서 그 영향을 관찰하였다. 태양전지의 경우 p충과 n충은 PECVD로 증착하였으며 단위박막 및 태양전지 i충 증착시 기판과 열선간의 거리는 7cm, 기판 온도는 200?C와 250?C로 고정하였고 작업압력은 30mTorr였다. 단위 박막 특성 평가를 위해 암/광 전도도, SEM, Raman Scattering, FT-IR등을 사용하였으며 태양전지 특성 평가를 위해 I-V 및 Spectral response를 측정하였다. 열선 온도가 증가함에 따라 증착속도 및 결정화 분율은 증가하였다. 특히 비정질에서 결정질로 전이되는 구간은 매우 좁았으며 여러 분석 방법에서 일치되는 결과를 보였다. SiH₄ 유량이 늘어날수록 비정질이 결정질로 바뀌는 열선 온도가 증가하였으며 기판 온도가 낮을 경우 또한 결정으로 바뀌는 열선 온도가 증가하였다. 태양전지의 경우 열선 온도가 증가함에 따라 V_{oc} 및 W가 낮아졌으며 J_{sc}, 는 증가하는 경향을 보였으며 결정질 비율이 증가하는 것을 관찰할 수 있었다. 이러한 경향은 quantum efficiency 결과에서도 확인할 수 있었다.
Song, Sun U.,Cha, Young-Deog,Han, Jeoung-Uk,Oh, In-Suk,Choi, Kyoung Baek,Yi, Youngsuk,Hyun, Jong-Pil,Lee, Hyeon-Youl,Chi, Guang Fan,Lim, Chae-Lyul,Ganjei, J. Kelly,Noh, Moon-Jong,Kim, Seong-Jin,Lee, D Mary Ann Liebert, Inc 2005 Tissue engineering Vol.11 No.9-10
<P>The purpose of this study was to investigate the efficacy of cartilage regeneration when using a mixture of transforming growth factor-beta1 (TGF-beta1)-producing human chondrocytes (hChon-TGF-beta1) and primary human chondrocytes (hChon) ('mixed cells'), compared with either hChon-TGF-beta1 or hChon cells alone. Specifically, mixed cells or hChon cells were first injected intradermally into the backs of immune-deficient nude mice to test the feasibility of cartilage formation in vivo. Both the mixed cells and the hChon-TGF-beta1 cells alone induced cartilage formation in nude mice, whereas hChon cells alone did not. To further test the efficacy of the cells in generating cartilage, an artificially induced partial thickness defect of the femoral condyle of a rabbit knee joint was loaded with hChon-TGF-beta1 cells with or without mixing additional untransduced hChon cells, and hyaline cartilage regeneration was observed at 4 or 6 weeks. The efficiency of complete filling of the defect and the quality of tissue generated after implanting were evaluated on the basis of a histological grading system modified from O'Driscoll et al. (J. Bone Joint Surg. 70A, 595, 1988). Significantly, mixed cells (14.2 +/- 0.9) produced significantly better results than hChon-TGF-beta1 (9.0 +/- 1.7) or hChon (8.0 +/- 1.8) cells alone. Histological and immunohistochemical staining of the newly repaired tissues produced after treatment with either mixed cells or hChon-TGF-beta1 cells alone showed hyaline cartilage- like characteristics. These results suggest that the implantation of mixed cells may be a clinically efficient method of regenerating hyaline articular cartilage.</P>