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Colorimetric and Fluorescent Recognition of Fluoride by a Binaphthol Thioureido Derivative
Li-Jun Tang,Nannan Wang,Jiaojiao Guo 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.7
A new thiourea based receptor (1) was synthesized and applied to fluoride ion recognition in acetonitrile solution. Receptor 1 displayed dual changes in absorption and fluorescence emission intensities selectively for fluoride ions. The interaction of 1 with fluoride undergoes a deprotonation process that is confirmed by 1H NMR titration.
JIAOJIAO DU,QIANG WANG,GUOJIAN LI,YONGZE CAO,JICHENG HE 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.2
This paper studies the e®ects of a high magnetic feld on the structural evolution and magneticproperties of nanocrystalline Ni flms prepared on quartz substrates by a molecular beam vapordeposition (MBVD) method. Atomic force microscope, X-ray di®ractometer, transmission elecftron microscope and vibrating sample magnetometer were used to study the microstructures andmagnetic properties of the Ni flms. The results indicate that high magnetic feld has no obviousinfluence on crystal structures except changing the lattice constant of the Ni flms. However, thehigh magnetic feld can refne particle size. The flm deposited under magnetic feld tends to growthrough columnar mode because of the magnetized particles aligning along the direction ofmagnetic feld. Furthermore, the ordered and dense arrangement of Ni atoms results in more spinscontained in per unit volume and improves the saturation magnetization (Ms). Ms of the 6 T Ni¯lm increases by 70% (578 emu/cm3) than that of the ¯lm without magnetic ¯eld (341 emu/cm3),and the coercivity is also slightly increased for the 6 T flm.
Jiaojiao Zheng,Hong Zhang,Tianyu Cao,Yuanyuan Zhu,Lizhong He,Jianwei Li,Xudong Chen,Yinhu Qu 한국섬유공학회 2022 Fibers and polymers Vol.23 No.10
Superhydrophobic surfaces by creating surface roughness followed by coating with low-surface-energyfluorinated materials have been intensively developed on cotton fabrics. However, exploring superhydrophobic surfaces withnon-fluorine components and further improving their attachment with cotton fabrics is still challenging. Herein, wedeveloped a superhydrophobic surface on cotton fabric by dip coating with hexadecylamine (HDA) modified coppernanowires (Cu NWs) based on the coordination between Cu atoms and amine groups. The HDA modified Cu NWs not onlycreated surface roughness, but also decreased the surface energy, leading to a superhydrophobic cotton fabric with watercontact angle of 164 °±2.0 °. Furthermore, ultrasonic washing and tape peeling tests exhibited the Cu NWs were adheredtightly to the surface of cotton fibers, owing to the strong hydrogen bond between amine groups on HDA molecules andhydroxyl groups on cotton fibers. In addition, the superhydrophobic cotton fabric showed excellent antifouling, oil-waterseparation and infrared reflection properties. It is believed that this facile method provide promising industrial applicationsfor fabricating superhydrophobic surfaces on other substrates.
Shuang Li,Bo Wang,Shun Jiang,Xiaohui Lan,Yongbo Qiao,Jiaojiao Nie,Yuhe Yin,Yuhua Shi,Wei Kong,Yaming Shan 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.1
Background: Porcine circovirus type 2 (PCV2) is an important infectious pathogen implicated in porcine circovirus-associated diseases (PCVAD), which has caused significant economic losses in the pig industry worldwide. Objectives: A suitable viral vector-mediated gene transfer platform for the expression of the capsid protein (Cap) is an attractive strategy. Methods: In the present study, a recombinant adeno-associated virus 8 (rAAV8) vector was constructed to encode Cap (Cap-rAAV) in vitro and in vivo after gene transfer. Results: The obtained results showed that Cap could be expressed in HEK293T cells and BABL/c mice. The results of lymphocytes proliferative, as well as immunoglobulin G (IgG) 2a and interferon-γ showed strong cellular immune responses induced by Cap-rAAV. The enzyme-linked immunosorbent assay titers obtained and the IgG1 and interleukin-4 levels showed that humoral immune responses were also induced by Cap-rAAV. Altogether, these results demonstrated that the rAAV8 vaccine Cap-rAAV can induce strong cellular and humoral immune responses, indicating a potential rAAV8 vaccine against PCV2. Conclusions: The injection of rAAV8 encoding PCV2 Cap genes into muscle tissue can ensure long-term, continuous, and systemic expression.
Establishment of hybridization breeding of Ganoderma lucidum by protoplast monokaryogenesis method
Wu Jiaojiao,Fu Lizhong,Wu Xueqian,Xu Xiuhong,Li Haibo,Wu Qingqi,Wei Hailong,Cheng Junwen 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4
Four Ganoderma lucidum strains, Chizhi 05, Jingda, Huizhou and Xinzhou, were screened out as hybrid parent in order to establish G. lucidum cross breeding system that based on protoplast monokaryogenesis method. Monokaryotic strains of each parental strains were obtained and mating type of each monokaryotic strains were determined. One to three monokaryotic strains that have different mating types were mated, and hybrids were identified by clamp connection observation and antagonist response. The results showed that the number of monokaryon came from Chizhi 05, Jingda, Huizhou and Xinzhou was 9, 14, 40 and 38, respectively. Only one mating type was obtained from Jingda, and two mating types were obtained from the other three strains, Chizhi 05, Huizhou and Xinzhou, respectively. Chi-square test showed that the ratio of two mating types of the three strains was 1:1. Fourteen monokaryotic strains of different mating types from 4 parental strains were select as a cross- breeding materia, and 17 hybrids were obtained, which were identified by clamp connection observation and antagonist response. This study proclaimed that the practicality of the hybridization breeding of G. lucidum by protoplast monokaryogenesis method.
Yang, Tianyi,Li, Jiaojiao,Li, Liangzhi,Zhang, Haiyang,Ma, Jing,Chen, Zhi,Hu, Cuiying,Ju, Xin,Fu, Jiaolong 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.6
Tacrolimus (FK506), a 23-membered polyketide macrolide with immunosuppressant activity, can be produced by Streptomyces tsukubaensis. We studied a variety of mutant strains of S. tsukubaensis for the microbial production of FK506. The best strain (CZ-19) was obtained from the parent strain LLZ-1 by nitrosoguanidine mutation and 4-Aminobutyric acid (FK506 precursor structure analogs) adaption. In the shake-flask experiments, titer of FK506 by CZ-19 was 532.44 mg/L, increased by 65.13% compared to that of the parent strain. Through single factor experiments and response surface methodology, we further optimized the medium for improved FK506 production by CZ-19 in shake flask culture. The optimal medium for enhanced FK506 production was as follows: 17.19 g/L corn starch, 21.78 g/L glucose, 8.06 g/L peptone, and 18.98 mg/L 4-aminobutyric acid. The predicted FK506 titer was 906.49 mg/L, and the experimental data confirmed the validity of the model. The present study demonstrates that S. tsukubaensis CZ-19 is a promising strain for industrial production of FK506.
Tianyi Yang,Jiaojiao Li,Liangzhi Li,Haiyang Zhang,Jing Ma,Zhi Chen,Cuiying Hu,Xin Ju,Jiaolong Fu 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.6
Tacrolimus (FK506), a 23-membered polyketidemacrolide with immunosuppressant activity, can be produced byStreptomyces tsukubaensis. We studied a variety of mutant strainsof S. tsukubaensis for the microbial production of FK506. Thebest strain (CZ-19) was obtained from the parent strain LLZ-1 bynitrosoguanidine mutation and 4-Aminobutyric acid (FK506precursor structure analogs) adaption. In the shake-flask experiments,titer of FK506 by CZ-19 was 532.44 mg/L, increased by 65.13%compared to that of the parent strain. Through single factorexperiments and response surface methodology, we furtheroptimized the medium for improved FK506 production by CZ-19in shake flask culture. The optimal medium for enhanced FK506production was as follows: 17.19 g/L corn starch, 21.78 g/Lglucose, 8.06 g/L peptone, and 18.98 mg/L 4-aminobutyric acid. The predicted FK506 titer was 906.49 mg/L, and the experimentaldata confirmed the validity of the model. The present studydemonstrates that S. tsukubaensis CZ-19 is a promising strain forindustrial production of FK506.
Lu Wang,Xia Li,Hongwei Niu,Lin Yang,Mingqin Xu, Jiaojiao Yi 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.10
Cr was used to replace Nb, Ti, and Zr in a precursor alloy HfMoNbTiZr, and its effect on the microstructure, phase constitution,and mechanical properties of the newly formed alloys were investigated. Microstructural evolutions revealed thata cubic Laves phase emerged regardless of which element was substituted by Cr. The original single-phase BCC structureof the precursor alloy was thus deteriorated and replaced by two BCC and one cubic Laves phases. The formation of theLaves phase and BCC phases increased the strength to 2480 ± 40 MPa in the HfMoNbTiCr alloy, while the net-like structure(Laves phase) impeded the crack extension and compensated for the decreased plasticity due to the introduction of thebrittle-stiff Laves phase. The other two alloys, HfMoTiZrCr and HfMoNbZrCr, possessed lower strengths of 1400 ± 20 and1642 ± 23 MPa and exhibited a completely brittle fracture character, which was attributed to the high volume fraction andthe morphologies of the Laves phase.
Jianhui Zhang,Jun Ge,Juyin Li,Jianqiang Li,Yong Zhang,Yinghui Shi,Jiaojiao Sun,Qiongjin Wang,Xiaobo Zhang,Xing-xu Zhao 대한수의학회 2023 Journal of Veterinary Science Vol.24 No.1
Background: Inactivated vaccines are limited in preventing foot-and-mouth disease (FMD) due to safety problems. Recombinant virus-like particles (VLPs) are an excellent candidate for a novel vaccine for preventing FMD, given that VLPs have similar immunogenicity as natural viruses and are replication- and infection-incompetent. Objectives: The 3C protease and P1 polyprotein of type O FMD virus (FDMV) was expressed in yeast Hansenula polymorpha to generate self-resembling VLPs, and the potential of recombinant VLPs as an FMD vaccine was evaluated. Methods: BALB/c mice were immunized with recombinant purified VLPs using CpG oligodeoxynucleotide and aluminum hydroxide gel as an adjuvant. Cytokines and lymphocytes from serum and spleen were analyzed by enzyme-linked immunosorbent assay, enzyme-linked immunospot assay, and flow cytometry. Results: The VLPs of FMD were purified successfully from yeast protein with a diameter of approximately 25 nm. The immunization of mice showed that animals produced high levels of FMDV antibodies and a higher level of antibodies for a longer time. In addition, higher levels of interferon-γ and CD4+ T cells were observed in mice immunized with VLPs. Conclusions: The expression of VLPs of FMD in H. polymorpha provides a novel strategy for the generation of the FMDV vaccine.