Simpler Efficient Group Signature Scheme with Verifier-Local Revocation from Lattices

( Yanhua Zhang ),( Yupu Hu ),( Wen Gao ),( Mingming Jiang ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.1

Verifier-local revocation (VLR) seems to be the most flexible revocation approaches for any group signature scheme, because it just only requires the verifiers to possess some up-to-date revocation information, but not the signers. Langlois et al. (PKC 2014) proposed the first VLR group signature based on lattice assumptions in the random oracle model. Their scheme has at least O(n²)·logN bit group public key and O (n) ·log N bit signature, respectively. Here, n is the security parameter and N is the maximum number of group members. In this paper, we present a simpler lattice-based VLR group signature, which is more efficient by a(log)ONfactor in both the group public key and the signature size. The security of our VLR group signature can be reduced to the hardness of learning with errors (LWE) and small integer solution (SIS) in the random oracle model.

Molecularly imprinted electrospun nanofibre membrane assisted stir bar sorptive extraction for trace analysis of sulfonamides from animal feeds

Yahan Cui,Liyan Jiang,Huiyu Li,Di Meng,Yanhua Chen,Lan Ding,Yang Xu 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.96 No.-

The widespread addition of sulfa antibiotics in feeds has long been an environment safety issue. Therefore, it is quite meaningful to constantly propose better solutions for determining the sulfaantibiotics in the environment. Herein, molecularly imprinted electrospun nanofibre membrane (MIM)assisted stir bar sorptive extraction method for the determination sulfonamides (SAs) in feed wasdeveloped. In this study, molecularly imprinted polymers (MIPs) werefirstly prepared via emulsionpolymerization using sulfamonomethoxine as template. Then, the morphology-controllable MIM wasobtained with MIPs-doping electro-spinning solution using electro-spinning technique. Next, MIM arecoated on the magnetic stir bar by heat-sealing. Finally, MIM coated stir bar was used for selectiveenrichment of SAs. The obtained MIM exhibited excellent class selectivity towards SAs (the selectivityfactor β is 2.3–2.7). The MIM coated stir bar can be directly applied to extract SAs from feed samples. Under the optimum extraction conditions, the detection limit of the method could reach 1.5 3.4 ng/g. And satisfactory recoveries ranging from 80 6% to 89 7% were achieved under all three spiked levelsfor determination of four SAs infive different feed samples. The present work not only offers insights todevelop magnetic stir bar using imprinted membrane, but also provides a new method for SAs extraction.

BMB : Reports ; An inhibitory role of NEK6 in TGFβ/Smad signaling pathway

( Jie Zuo ),( Haijie Ma ),( Hao Cai ),( Yanhua Wu ),( Wei Jiang ),( Long Yu ) 생화학분자생물학회(구 한국생화학분자생물학회) 2015 BMB Reports Vol.48 No.8

The NEK6 (NIMA-related kinases 6) is reported to play po-tential roles in tumorigenesis. Although it is suggested to function in several cellular pathways, the underlying mechanism in tumorigenesis is still largely unknown. In the present study, we discovered interaction of NEK6 with Smad4, a key member of transforming growth factor beta (TGFβ) pathway. Over-expression of NEK6 in hepatocellular carcinoma (HCC) cell lines suppresses TGFβ- mediated transcription activity in a kinase activity-dependent manner. In addition, NEK6 suppresses the cell growth arrest induced by TGFβ. Mechanically, NEK6 blocks nuclear translocation of Smad4, which is essential for TGF β function. Moreover, we identified that NEK6 could be regulated by TGFβ and hypoxia. Our study sheds new light on the roles of NEK6 in canonical TGFβ/Smad pathway and tum-origenesis. [BMB Reports 2015; 48(8): 473-478]

Protective effect of Lactobacillus plantarum ATCC8014 on acrylamide-induced oxidative damage in rats

Zhao Sijia,Zhao Xiaoduo,Liu Qingbo,Jiang Yujun,Li Yanhua,Feng Wenxiao,Xu Honghua,Shao Meili 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.4

Acrylamide (AA), which is mainly found in fried foods, causes neurotoxicity, genetic toxicity, carcinogenic effects, and DNA damage. This study confirms that a strain of lactic acid bacteria (Lactobacillus plantarum ATCC8014) could alleviate the toxicity of rats by inhibiting the AA-induced oxidative damage. Forty-eight adult male SD rats were randomly divided into eight groups: control group, AA group (40 mg/kg), three different doses (1 × 107 CFU/ml, 1 × 108 CFU/ ml, 1 × 109 CFU/ml of Lactobacillus plantarum ATCC8014) of prevention groups and therapeutic groups, respectively. At the end of three-week experiment, AA treatment produced a significant reduction in the rate of weight gain along with the symptoms of hind limb splay and ataxia. Histological examinations revealed various degrees of injury in five tissues. Levels of superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) in group AA rats were significantly decreased, but the level of lipid peroxidation (LPO) was significantly increased (p < 0.05). Both prevention and therapeutic groups with 1 × 109 CFU/ml of Lactobacillus plantarum ATCC8014 could effectively reduce the injury of AA to the body. However, reductions in both groups were not statistically significant.

Discovery of genes associated with cadmium accumulation from gill of scallop Chlamys farreri based on high-throughput sequencing

Yuxiu Zhai,Hui Zhang,Lin Yao,Yanhua Jiang,Fengling Li 한국유전학회 2016 Genes & Genomics Vol.38 No.5

Chlamys farreri is an important economic mollusk and is able to accumulate cadmium (Cd) excessively. Two cDNA libraries from gill of C. farreri which were Cd treated or not were generated based on the Illumina sequencing platform in order to identify differentially expressed genes (DEGs) associated with cadmium accumulation. The analysis of comparative transcriptomics identified 82,800 unigenes and 128 differentially expressed genes. Of these, 8 of the most significant DEGs were verified by real-time PCR, showing consistent trends in expression patterns between the two techniques. KEGG pathways analysis revealed that 37 associated processes were highly enriched. A set of key DEGs were identified that may play important roles in cadmium exposure. Our work presents an overview of gene expression change during cadmium exposure to C. farreri and identifies a series of candidate genes and pathways for further research on the molecular mechanisms of cadmium accumulation.

Function of VP2 Protein in the Stability of the Secondary Structure of Virus-like Particles of Genogroup II Norovirus at Different pH Levels: Function of VP2 Protein in the Stability of NoV VLPs

Yao Lin,Li Fengling,Wang Lianzhu,Zhai Yuxiu,Jiang Yanhua 한국미생물학회 2014 The journal of microbiology Vol.52 No.11

VP2 is the minor structural protein of noroviruses (NoV)and may function in NoV particle stability. To determinethe function of VP2 in the stability of the NoV particle, weconstructed and purified two kinds of virus-like particles(VLPs), namely, VLPs (VP1) and VLPs (VP1+VP2), fromSf9 cells infected with recombinant baculoviruses by usinga Bac-to-Bac® baculovirus expression system. The two kindsof VLPs were treated with different phosphate buffers (pH2 to pH 8); the secondary structure was then analyzed byfar UV circular dichroism (CD) spectroscopy. Results showedthat significant disruptions of the secondary structure ofproteins were not observed at pH 2 to pH 7. At pH 8, thepercentages of α-helix, β-sheet, and β-turn in VLPs (VP1)were decreased from 11% to 8%, from 37% to 32%, andfrom 20% to 16%, respectively. The percentage of coil wasincreased from 32% to 44%. By contrast, the percentages ofα-helix, β-sheet, and β-turn in VLPs (VP1+VP2) were decreasedfrom 11% to 10%, from 37% to 35%, and from 20%to 19%, respectively. The percentage of coil was increasedfrom 32% to 36%. VLPs (VP1+VP2) was likely more stablethan VLPs (VP1), as indicated by the percentage of the secondarystructures analyzed by CD. These results suggestedthat VP2 could stabilize the secondary structure of VLPsunder alkaline pH conditions. This study provided novelinsights into the molecular mechanism of the function ofVP2 in the stability of NoV particles.

MCT2 overexpression promotes recovery of cognitive function by increasing mitochondrial biogenesis in a rat model of stroke

Xiaorong Yu,Rui Zhang,Cunsheng Wei,Yuanyuan Gao,Yanhua Yu,Lin Wang,Junying Jiang,Xuemei Zhang,Junrong Li,Xuemei Chen 한국통합생물학회 2021 Animal cells and systems Vol.25 No.2

Monocarboxylate transporter 2 (MCT2) is the predominant monocarboxylate transporter expressed by neurons. MCT2 plays an important role in brain energy metabolism. Stroke survivors are at high risk of cognitive impairment. We reported previously that stroke-induced cognitive impairment was related to impaired energy metabolism. In the present study, we report that cognitive function was impaired after stroke in rats. We found that MCT2 expression, but not that of MCT1 or MCT4, was markedly decreased in the rat hippocampus at 7 and 28 days after transient middle cerebral artery occlusion (tMCAO). Moreover, MCT2 overexpression promoted recovery of cognitive function after stroke. The molecular mechanism underlying these effects may be related to an increase in adenosine monophosphate-activated protein kinase-mediated mitochondrial biogenesis induced by overexpression of MCT2. Our findings suggest that MCT2 activation ameliorates cognitive impairment after stroke.