RUNX1 Ameliorates Rheumatoid Arthritis Progression through Epigenetic Inhibition of LRRC15

Jian-ning Zhao,Hao Ding,Xiaoliang Mei,Lintao Li,Peng Fang,Ting Guo 한국분자세포생물학회 2023 Molecules and cells Vol.46 No.4

Leucine-rich repeat containing 15 (LRRC15) has been identified as a contributing factor for cartilage damage in osteoarthritis; however, its involvement in rheumatoid arthritis (RA) and the underlying mechanisms have not been well characterized. The purpose of this study was to explore the function of LRRC15 in RA-associated fibroblastlike synoviocytes (RA-FLS) and in mice with collagen-induced arthritis (CIA) and to dissect the epigenetic mechanisms involved. LRRC15 was overexpressed in the synovial tissues of patients with RA, and LRRC15 overexpression was associated with increased proliferative, migratory, invasive, and angiogenic capacities of RA-FLS and accelerated release of pro-inflammatory cytokines. LRRC15 knockdown significantly inhibited synovial proliferation and reduced bone invasion and destruction in CIA mice. Runt-related transcription factor 1 (RUNX1) transcriptionally represses LRRC15 by binding to core-binding factor subunit beta (CBF-β). Overexpression of RUNX1 significantly inhibited the invasive phenotype of RA-FLS and suppressed the expression of proinflammatory cytokines. Conversely, the effects of RUNX1 were significantly reversed after overexpression of LRRC15 or inhibition of RUNX1-CBF-β interactions. Therefore, we demonstrated that RUNX1-mediated transcriptional repression of LRRC15 inhibited the development of RA, which may have therapeutic effects for RA patients.

Parametric Research on Underactuated Tendon-Driven Grasping Mechanism for Space Capture Operation

Jian Tian,Cheng Wei,Min Luo,Ning Wang,Chunlin Tan,Yang Zhao 한국정밀공학회 2020 International Journal of Precision Engineering and Vol.21 No.2

Space objects always stay in rotation mode with various nutation according to their different inertia parameters during free flying. It is hard for designing a grasping mechanism to capture a rotating target with unknown velocity and unknown size. An under-actuated tendon-driven grasping mechanism is designed and researched in this paper, and which could handle the uncertainties during the capturing using its structural flexibility and strong adaption to space unknown rotating objects for taking the advantage of under-actuation. The under-actuated grasping mechanism consists of four fingers, and each finger has four joints, but driven by only one tendon and four pre-designed springs, which make it a typical under-actuated mechanism. The partial feedback linearization method is modified for the control with time varying constraints and the parametric design method is proposed to complete the tracking control of multiple joint angle using a single driven tendon, and which is validated by the simulation of the whole capturing procedure.

Comparison of Apixaban and Low Molecular Weight Heparin in Preventing Deep Venous Thrombosis after Total Knee Arthroplasty in Older Adults

Ting Guo,Jian-ning Zhao,Hui Jiang,Jia Meng,Yi-cun Wang,Jun Wang,Yang Qiu,Hao Ding 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.7

Purpose: To compare the effect of apixaban and low molecular weight heparin (LMWH) in the prevention and treatment of deepvenous thrombosis (DVT) after total knee arthroplasty in older adult patients. Materials and Methods: A total of 220 patients (average age of 67.8±6.4 years) undergoing total knee arthroplasty were randomlyselected as research subjects and were divided into apixaban and LMWH groups (110 in each group). Results: The incidence of DVT was lower in the apixaban group than in the LMWH group (5.5% vs. 20.0%, p=0.001). Activatedpartial thromboplastin times (35.2±3.6 sec vs. 33.7±2.2 sec, p=0.010; 37.8±4.6 sec vs. 34.1±3.2 sec, p<0.001; 39.6±5.1 sec vs. 35.7±3.0sec, p=0.032) and prothrombin times (14.0±1.0 sec vs. 12.8±0.9 sec, p<0.001; 14.5±1.2 sec vs. 13.0±1.1 sec, p<0.001; 15.3±1.4 sec vs. 13.2±1.3 sec, p=0.009) in the apixaban group at 1 week after surgery, 3 weeks after surgery, and the end of treatment were higherthan those in the LMWH group. Platelet and fibrinogen levels in the apixaban group were lower than those of the LMWH group. Also, capillary plasma viscosity and erythrocyte aggregation in the apixaban group at 1 week after surgery, 3 weeks after surgery,and the end of treatment were lower than those in the LMWH group. Conclusion: Apixaban, which elicits fewer adverse reactions and is safer than LMWH, exhibited better effects in the preventionand treatment of DVT after total knee arthroplasty in older adults.

Controlled Growth of Well-Defined Conjugated Polymers from the Surfaces of Multiwalled Carbon Nanotubes: Photoresponse Enhancement <i>via</i> Charge Separation

Hou, Wenpeng,Zhao, Ning-Jiu,Meng, Dongli,Tang, Jing,Zeng, Yi,Wu, Yu,Weng, Yangziwan,Cheng, Chungui,Xu, Xiulai,Li, Yi,Zhang, Jian-Ping,Huang, Yong,Bielawski, Christopher W.,Geng, Jianxin American Chemical Society 2016 ACS NANO Vol.10 No.5

<P>The installation of heterojunctions on the surfaces of carbon nanotubes (CNTs) is an effective method for promoting the charge separation processes needed for CNT-based electronics and optoelectronics applications. Conjugated polymers are proven state-of-the-art candidates for modifying the surfaces of CNTs. However, all previous attempts to incorporate conjugated polymers to CNTs resulted in unordered interfaces. Herein we show that well-defined chains of regioregular poly(3-hexylthiophene) (P3HT) were successfully grown from the surfaces of multiwalled CNTs (MWNTs) using surface-initiated Kumada catalyst-transfer polycondensation. The polymerization was found to proceed in a controlled manner as chains of tunable lengths were prepared through variation of the initial monomer-to-initiator ratio. Moreover, it was determined that large-diameter MWNTs afforded highly ordered P3HT aggregates, which exhibited a markedly bathochromically shifted optical absorption due to a high grafting density induced planarization of the polymer chains. Using ultrafast spectroscopy, the heterojunctions formed between the MWNTs and P3HT were shown to effectively overcome the binding energy of excitons, leading to photoinduced electron transfer from P3HT to MWNTs. Finally, when used as prototype devices, the individual MWNT-g-P3HT core-shell structures exhibited excellent photoresponses under a low illumination density.</P>

Real-Time Contour-Error Estimation Methods for Three-Dimensional Free-Form Parametric Curves in Contour-Following Tasks

Zhen-yuan Jia,De-Ning Song,Jian-Wei Ma,Xiao-Xuan Zhao,Ning Zhang 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.19 No.2

It is crucial to control the contour error in curved contour-following tasks caused by reasons such as servo delay and external disturbance. Contour-error estimation plays as a precondition for its further control. Existing methods can hardly keep well estimation accuracy for high-speed following of free-form curves with sharp corners, especially for three-dimensional curves. Consequently, this paper presents three high-precision real-time contour-error estimation methods for spatial free-form parametric curved contour following. By generating and updating the backstepping point according to the tangential tracking error, a multiple tangential approximation method is presented first. Then, a spatial circular approximation method is given by means of approximating the actual-position nearby region of the desired contour with a spatial circle. Finally, via modification of the Newton method so as to improve its stability without sacrificing of its fast convergence property, an initial value regeneration-based Newton algorithm is proposed for contour-error estimation. All of the presented methods take both estimation precision and calculation burden into consideration, and possess their own advantages. Using these algorithms, the contour error can be rapidly estimated in vector form with a high accuracy. Simulation and experimental results demonstrate the feasibility and the superiority of the presented algorithms.

Effects of Tissue Factor, PAR-2 and MMP-9 Expression on Human Breast Cancer Cell Line MCF-7 Invasion

Lin, Zeng-Mao,Zhao, Jian-Xin,Duan, Xue-Ning,Zhang, Lan-Bo,Ye, Jing-Ming,Xu, Ling,Liu, Yin-Hua Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Objective: This study aimed to explore the expression of tissue factor (TF), protease activated receptor-2 (PAR-2), and matrix metalloproteinase-9 (MMP-9) in the MCF-7 breast cancer cell line and influence on invasiveness. Methods: Stable MCF-7 cells transfected with TF cDNA and with TF ShRNA were established. TF, PAR-2, and MMP-9 protein expression was analyzed using indirect immunofluorescence and invasiveness was evaluated using a cell invasion test. Effects of an exogenous PAR-2 agonist were also examined. Results: TF protein expression significantly differed between the TF cDNA and TF ShRNA groups. MMP-9 protein expression was significantly correlated with TF protein expression, but PAR-2 protein expression was unaffected. The PAR-2 agonist significantly enhanced MMP-9 expression and slightly increased TF and PAR-2 expression in the TF ShRNA group, but did not significantly affect protein expression in MCF-7 cells transfected with TF cDNA. TF and MMP-9 expression was positively correlated with the invasiveness of tumor cells. Conclusion: TF, PAR-2, and MMP-9 affect invasiveness of MCF-7 cells. TF may increase MMP-9 expression by activating PAR-2.

Characteristics of Mammary Paget's Disease in China: a National-wide Multicenter Retrospective Study During 1999-2008

Zheng, Shan,Song, Qing-Kun,Zhao, Lin,Huang, Rong,Sun, Li,Li, Jing,Fan, Jin-Hu,Zhang, Bao-Ning,Yang, Hong-Jian,Xu, Feng,Zhang, Bin,Qiao, You-Lin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

The aim of this study was to detail characteristics of mammary Paget's disease (PD) representing the whole population in China. A total of 4211 female breast cancer inpatients at seven tertiary hospitals from seven representative geographical regions of China were collected randomly during 1999 to 2008. Data for demography, risk factors, diagnostic imaging test, physical examination and pathologic characters were surveyed and biomarker status was tested by immunohistochemistry. The differences of demography and risk factors between PD with breast cancer and other lesions were compared using Chi-square test or t-test, with attention to physical examination and pathological characters. The percentage of PD was 1.6% (68/4211) in all breast cancers. The mean age at diagnosis was 48.1, and 63.2% (43/68) patients were premenopausal. There is no difference in demography and risk factors between PD with breast cancer and other breast cancer (P > 0.05). The main pattern of PD in physical exam and pathologic pattern were patients presenting with a palpable mass in breast (65/68, 95.6%) and PD with underlying invasive cancer (82.4%, 56/68) respectively. The rate of multifocal disease was 7.4% (5/68). PD with invasive breast cancer showed larger tumor size, more multifocal disease, lower ER and PR expression and higher HER2 overexpression than those in other invasive breast cancer (P < 0.05). These results suggested that PD in China is a concomitant disease of breast cancer, and that PD with underlying invasive cancer has more multiple foci and more aggressive behavior compared with other breast invasive cancer. We address the urgent needs for establishing diagnostic and therapeutic guidelines for mammary PD in China.

Utrecht Interstitial Applicator Shifts and DVH Parameter Changes in 3D CT-based HDR Brachytherapy of Cervical Cancer

Shi, Dan,He, Ming-Yuan,Zhao, Zhi-Peng,Wu, Ning,Zhao, Hong-Fu,Xu, Zhi-Jian,Cheng, Guang-Hui Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9

Background: For brachytherapy of cervical cancer, applicator shifts can not be avoided. The present investigation concerned Utrecht interstitial applicator shifts and their effects on organ movement and DVH parameters during 3D CT-based HDR brachytherapy of cervical cancer. Materials and Methods: After the applicator being implanted, CT imaging was achieved for oncologist contouring CTVhr, CTVir, and OAR, including bladder, rectum, sigmoid colon and small intestines. After the treatment, CT imaging was repeated to determine applicator shifts and OARs movements. Two CT images were matched by pelvic structures. In both imaging results, we defined the tandem by the tip and the base as the marker point, and evaluated applicator shift, including X, Y and Z. Based on the repeated CT imaging, oncologist contoured the target volume and OARs again. We combined the treatment plan with the repeated CT imaging and evaluated the change range for the doses of CTVhr D90, D2cc of OARs. Results: The average applicator shift was -0.16 mm to 0.10 mm for X, 1.49 mm to 2.14 mm for Y, and 1.9 mm to 2.3 mm for Z. The change of average physical doses and EQD2 values in Gy${\alpha}/{\beta}$ range for CTVhr D90 decreased by 2.55 % and 3.5 %, bladder D2cc decreased by 5.94 % and 8.77 %, rectum D2cc decreased by 2.94 % and 4 %, sigmoid colon D2cc decreased by 3.38 % and 3.72 %, and small intestines D2cc increased by 3.72 % and 10.94 %. Conclusions: Applicator shifts and DVH parameter changes induced the total dose inaccurately and could not be ignored. The doses of target volume and OARs varied inevitably.