Berberine suppresses in vitro migration of human aortic smooth muscle cells through the inhibitions of MMP-2/9, u-PA, AP-1, and NF-κB

( Su Jian Liu ),( Cai Xia Yin ),( Ming Chao Ding ),( Shao You Xia ),( Qin Min Shen ),( Ji Dong Wu ) 생화학분자생물학회(구 한국생화학분자생물학회) 2014 BMB Reports Vol.47 No.7

Berberine, a type of isoquinoline alkaloid isolated from Chinese medicinal herbs, has been reported to have various pharmacological activities. Studies have demonstrated that berberine has beneficial effects on vascular remodeling and alleviates restenosis after vascular injury. However, its mechanism of action on vascular smooth muscle cell migration is not fully understood. We therefore investigated the effect of berberine on human aortic smooth muscle cell (HASMC) migration. Boyden chamber assay was performed to show that berberine inhibited HASMC migration dose- dependently. Real-time PCR and Western blotting analyses showed that levels of matrix metalloproteinase (MMP)-2, MMP-9, and urokinase-type plasminogen activator (u-PA) were reduced by berberine at both the mRNA and protein levels. Western blotting assay further confirmed that activities of c-Fos, c-Jun, and NF-κB were significantly attenuated. These results suggest that berberine effectively inhibited HASMC migration, possibly by down-regulating MMP-2, MMP-9, and u-PA; and interrupting AP-1 and NF-κB mediated signaling pathways. [BMB Reports 2014; 47(7): 388-392]

Cultivation of Nostoc flagelliforme on Solid Medium

( Jian Yu Su ),( Shi Ru Jia ),( Chang Sheng Qiao ),( Jung Gyu Kim ),( Wan Hae Hong ),( Ki An Cho ),( Du Bok Choi ) 한국환경생물학회 2005 환경생물 : 환경생물학회지 Vol.23 No.2

N/A In order to construct an artificial cultivation of Nostoc flagelliforme on solid medium, we attempted to assess the viability of approaches, which utilized either BG -11 agar or sand medium using both sterile and non-sterile algal segments. In the trial in which the BG-11 agar medium was inoculated with the non-sterile algal segments, the algae exhibited the rapid growth in the initial 4 days of cultivation. However, after 4 days of cultivation, the growth rate of the algae slowed, and the algal growth was completely stopped by 7 days of cultivation. When the BG-11 medium was inoculated with the sterile algal segments, the algae exhibited the rapid growth for a longer period of 8 days, reaching a length of 24.9 mm. The growth rate during this period was measured to be 24.5%. After the 8 days of cultivation, the algal growth rate began to slow and had almost stopped by the 13 days of cultivation. On the other hand, when the sterile algal segments were inoculated onto a sand plate, the algal segments decomposed, reaching total decomposition after 11 days of cultivation. By way of contrast, the desiccation treatment samples continued to grow for 14 days of cultivation. After 14 days of cultivation, the algae achieved a length of 26.1 mm, with a growth rate of 30.6%. Our results indicate that periodic desiccation may constitute an effective strategy for the prevention of algal decomposition.

Redundant rule Detection for Software-Defined Networking

( Jian Su ),( Ruoyu Xu ),( Shiming Yu ),( Baowei Wang ),( Jiuru Wang ) 한국인터넷정보학회 2020 KSII Transactions on Internet and Information Syst Vol.14 No.6

The emergence of Software Defined Networking (SDN) overcomes the limitations of traditional networking architectures. There are some advantages in SDN which are centralized global network view, programmability, and separation of the data plane and control plane. Due to the limitation of data plane storage capacity in SDN, it is necessary to process the redundancy rules of switch. In this paper, we propose a method for active detection and processing of redundant rules. We use the result generated by the customized probe package to detect redundant rules. And by checking the forwarding behavior of probe packets in the data plane, the redundancy rules are further processed. Furthermore, in order to quickly check the dynamic networks, we propose an incremental algorithms for rapidly evolve the network strategies. We conduct simulation experiments on Matlab to verify the feasibility of the algorithm. The influence of some parameters on the result are discussed.

Eliminating the Third Harmonic Effect for Six Phase Permanent Magnet Synchronous Generators in One Phase Open Mode

Jian Liu,Gui-jie Yang,Yong Li,Hong-wei Gao,Jian-yong Su 전력전자학회 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.1

To insure stable operation and eliminate twice torque ripple, a topology for a six phase permanent magnet synchronous generator (SP-PMSG) with a neutral point connected together was analyzed in this paper. By adopting an extended transformation matrix, the mathematic model of the space vector control was established. The voltage and torque equations were deduced while considering the third harmonic flux and inductance. In addition, the suppression third harmonic method and the closed loop control strategy were proposed. A comparison analysis indicates that the cooper loss minimum method and the current magnitude minimum method can meet different application requirements. The voltage compensation amount for each of the methods was deduced which also takes into account the third harmonic effect. A simulation and experimental result comparison validates the consistency through theoretical derivation. It can be seen that all of the two control strategies can meet the requirements of post-fault.

Ab Initio and Experimental Studies on Dibenzothiazyl-Disulfide

Jian, Fang-Fang,Zhang, Ke-Jie,Zhao, Pu-Su,Zheng, Jian Korean Chemical Society 2006 Bulletin of the Korean Chemical Society Vol.27 No.7

Ab initio calculations of the structure, atomic charges and natural bond orbital (NBO) have been performed at HF/6-311G** and B3LYP/6-311G** levels for the title compound of dibenzothiazyl-disulfide. The calculated results show that the two nitrogen atoms have the biggest negative charges and they are the potential sites to react with the metallic ions, which make the title compound become a di-dentate ligand. Vibrational frequencies of the title compound have been obtained and compared with the experimental value and the comparison indicates that B3LYP/6-311G** level is better than HF/6-311G** level to predict the vibrational frequencies for the system studied here. For the title compound, electronic absorption spectra calculated by time?ependent density functional theory (TD-DFT) are more accurate than Hartree-Focksingle-excitation CI (CI-Singles) method. NBO analyses show that the electronic transitions are mainly derived from the contribution of bands $\pi\rightarrow\pi^{*}$. Thermodynamic calculated results show that the formation of the title compound from 2-mercaptobenzothiazole is a spontaneous process at room temperature with the change of free Gibbs being negative value.

Idle Slots Skipped Mechanism based Tag Identification Algorithm with Enhanced Collision Detection

( Jian Su ),( Ruoyu Xu ),( Shiming Yu ),( Baowei Wang ),( Jiuru Wang ) 한국인터넷정보학회 2020 KSII Transactions on Internet and Information Syst Vol.14 No.5

In this article, a new Aloha-based tag identification protocol is presented to improve the reading efficiency of the EPC C1 Gen2-based UHF RFID system. Collision detection (CD) plays a vital role in tag identification process which determines the efficiency of anti-collision protocols since most Aloha-based protocols optimize the incoming frame length based on the collisions in current frame. Existing CD methods are ineffective in identifying collision, resulting in a degradation of identification performance. Our proposed algorithm adopts an enhanced CD (ECD) scheme based on the EPC C1 Gen2 standard to optimize identification performance. The ECD method can realize timely and effective CD by detecting the pulse width of the randomly sent by tags. According to the ECD, the reader detects the slot distribution and predicts tag cardinality in every collision slot. The tags involved in each collision slot are identified by independently assigned sub-frames. A large number of numerical results show that the proposed solution is superior to other existing anti-collision protocols in various performance evaluation metrics.

Decitabine in the Treatment of Acute Myeloid Leukemia and Myelodysplastic Syndromes, Which Combined with Complex Karyotype Respectively

Gao, Su,Li, Zheng,Fu, Jian-Hong,Hu, Xiao-Hui,Xu, Yang,Jin, Zheng-Ming,Tang, Xiao-Wen,Han, Yue,Chen, Su-Ning,Sun, Ai-Ning,Wu, De-Pei,Qiu, Hui-Ying Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.15

Background: We conducted a study exploring the clinical safety and efficacy of decitabine in patients with acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS), combined with a complex karyotype. Materials and Methods: From April 2009 to September 2013, a total of 35 patients with AML/MDS combined with a complex karyotype diagnosed in the First Affiliated Hospital of Soochow University were included for retrospective analysis. All patients were treated with decitabine alone ($20mg/m^2$ daily for 5 days) or combination AAG chemotherapy (Acla 20mg qod*4d, Ara-C $10mg/m^2$ q12h*7d, G-CSF $300{\mu}g$ qd, the dose of G-CSF adjusted to the amount in blood routinely). Results: In 35 patients, 15 exhibited a complete response (CR), and 6 a partial response (PR), the overall response rate (CR+PR) being 60% (21 of 35). Median disease-free survival was 18 months and overall survival was 14 months. In the 15 MDS patients with a complex karyotype, the CR rate was 53.3% (8 of 15); in 20 AML patients with complex karyotype, the overall response rate was 65% (13 of 20). The response rate of decitabine alone (22 cases) was 56.5% (13 of 22), while in the combination chemotherapy group (13 cases), the effective rate was 61.5% (8 of 13)(P>0.05). There are 15 patients with chromosome 7 aberration, after treatment with decitabine, 7 CR, 3 PR, overall response rate was 66.7% (10 of 15). Of 18 patients with 3 to 5 kinds of chromosomal abnormalities, 66.7% demonstrated a response; of 17 with more than 5 chromosomal abnormalities, 52.9% had a response. In the total of 35 patients, with one course (23 patients) and ${\geq}$two courses (12 patients), the overall response rate was 40.9% and 92.3% (P<0.05). Grade III to IV hematological toxicity was observed in 27 cases (75%). Grade III to IV infections were clinically documented in 7 (20%). Grades I to II non-hematological toxicity were infections (18 patients), haematuria (2 patients), and bleeding (3 patients). With follow-up until September 2013, 7 patients were surviving, 18 had died and 10 were lost to follow-up. In the 6 cases who underwent allogeneic hematopoietic stem cell transplantation (HSCT) all were still relapse-free survivors. Conclusions: Decitabine alone or combination with AAG can improve outcome of AML/MDS with a complex karyotype, there being no significant difference decitabine in inducing remission rates in patients with different karyotype. Increasing the number of courses can improve efficiency. This approach with fewer treatment side effects in patients with a better tolerance should be employed in order to create an improved subsequent chance for HSCT.

Growth, Clonability, and Radiation Resistance of Esophageal Carcinoma-derived Stem-like Cells

Li, Jian-Cheng,Liu, Di,Yang, Yan,Wang, Xiao-Ying,Pan, Ding-Long,Qiu, Zi-Dan,Su, Ying,Pan, Jian-Ji Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.8

Objective: To separate/enrich tumor stem-like cells from the human esophageal carcinoma cell line OE-19 by using serum-free suspension culture and to identify their biological characteristics and radiation resistance. Methods: OE-19 cells were cultivated using adherent and suspension culture methods. The tumor stem-like phenotype of CD44 expression was detected using flow cytometry. We examined growth characteristics, cloning capacity in soft agar, and radiation resistance of 2 groups of cells. Results: Suspended cells in serum-free medium formed spheres that were enriched for CD44 expression. CD44 was expressed in 62.5% of suspended cells, but only in 11.7% of adherent cells. The suspended cells had greater capacity for proliferation and colony formation in soft agar than the adherent cells. When the suspended and adherent cells were irradiated at 5 Gy, 10 Gy, or 15 Gy, the proportion of CD44+ suspended cells strongly and weakly positive for CD44 was 77.8%, 66.5%, 57.5%; and 21.7%, 31.6%, 41.4%, respectively. In contrast, the proportion of CD44+ adherent cells strongly positive for CD44 was 18.9%, 14.%, and 9.95%, respectively. When the irradiation dose was increased to 30 Gy, the survival of the suspended and adherent cells was significantly reduced, and viable CD44+ cells were not detected. Conclusion: Suspended cell spheres generated from OE-19 esophageal carcinoma cells in serum-free stem medium are enriched in tumor stem-like cells. CD44 may be a marker for these cells.

Eliminating the Third Harmonic Effect for Six Phase Permanent Magnet Synchronous Generators in One Phase Open Mode

Liu, Jian,Yang, Gui-Jie,Li, Yong,Gao, Hong-Wei,Su, Jian-Yong The Korean Institute of Power Electronics 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.1

To insure stable operation and eliminate twice torque ripple, a topology for a six phase permanent magnet synchronous generator (SP-PMSG) with a neutral point connected together was analyzed in this paper. By adopting an extended transformation matrix, the mathematic model of the space vector control was established. The voltage and torque equations were deduced while considering the third harmonic flux and inductance. In addition, the suppression third harmonic method and the closed loop control strategy were proposed. A comparison analysis indicates that the cooper loss minimum method and the current magnitude minimum method can meet different application requirements. The voltage compensation amount for each of the methods was deduced which also takes into account the third harmonic effect. A simulation and experimental result comparison validates the consistency through theoretical derivation. It can be seen that all of the two control strategies can meet the requirements of post-fault.

A Study on the Development of E-Commerce Shipping Platform in China

Lou Ying,Su-Ho Lee,Shou Jian-Min 한국항해항만학회 2016 한국항해항만학회지 Vol.40 No.2

With the advent of Internet era, e-commerce has become the focus of human's life. It leads a new direction of social development . As the representative of traditional industry, shipping industry is confronted with a series of difficulties, which have to break through the traditional and existing model to make their business for survival. With the increasing pricking up of market competition, shipping industry is now facing development bottle neck, but e-commerce provides a new way to solve the problem. This paper firstly describes the existing forms of the e-commerce shipping platform. Secondly analyzes the data for the situation of shipping industry in China, the data for expected functions of an e-commerce shipping platform and the data for how to choose a specific e-commerce shipping platform. Thirdly analyzes the potential risks of establishing e-commerce shipping platform in China. Based on the above researched, the paper provides a suggested model of the shipping e-commerce shipping platform in China.