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Production of Heterologous Protein from Pichia pasteris by Air Lift Bioreactor
Choi, Du Bok,Kim, Sun Il 한국공업화학회 2005 Journal of Industrial and Engineering Chemistry Vol.11 No.3
For high-level expression of heterologous protein using a methylotrophic yeast, P. pastoris, in an air lift bioreactor, the initial methanol concentration and inoculation time were investigated. When 30 g/L of methanol was used, α-amylase activity was highest (235 U/mL). When the inoculation was carried out at 18 hr of culture, the maximum α-amylase activity was obtained (270 U/mL) and the consumed methanol concentration was 24.S g/L. The α-amylase activity expressed in P. pastrris was stable up to 50℃ and showed 86.4% of residual activity at 60℃. However. in the case of S. cererisiae, it remained only 57.3% under the same conditions. Between pH 6 and 7, the mouse α-amylase activity expressed in P. pcesteris was stable. However, in the case of S. cerehiciae, it was stable between pH 7 and 8. Using the optimum culture conditions, the fed cultures were carried out for 4 days. The methanol consumption increased upon culture time up to 3 day s, but after 4 days it began to decrease. The cell concentration was similar to that of S. cerevisiae. The maximum α-amylase activity was 610 U/mL at 4 days of culture; this value was about 6.0-fold higher than that of S. cerevisiae. In the case of product yield from methanol, it was 15.2 U/mL/g the consumed carbon source, which was about 11.5-fold higher than that of S. cerevisiae using glucose.
Functional properties of hot water extract of a fish, seaweed, and mushroom mixture
Du Bok Choi,차월석 한국화학공학회 2011 Korean Journal of Chemical Engineering Vol.28 No.5
The purpose of this study was to evaluate the effect of hot water extracts of a fish, seaweed, and mushroom mixture (FSM extract) on the antioxidant and anticancer activities for use as health and functional food resources. DPPH radical scavenging activity increased from 13.4 to 93.5% when the FSM extract concentration ranged from 0.5 to 25 mg/mL. The reducing power increased from 0.04 to 1.06 OD 700 nm when the FSM extract concentration increased from 0.25 to 10 mg/mL. Nitrite scavenging activity increased from 10.3 to 96.9% when the FSM extract concentration increased from 1 to 25 mg/mL. The activities of alcohol dehydrogenase and acetaldehyde dehydrogenase in the FSM extractfed group were 2.45 and 6.12 units/min, respectively. The activities of CAT, SOD, and GSH-Px in the FSM extractfed group were 19.7 units/mg protein, 11.5 units/mg protein, and 16.9 units/mg protein/min, respectively. Cell viabilities of SNU213, SNU324, and SNU354 were 7.5, 9.4, and 8.9%, respectively. Cell viabilities of SNU719, SNU1, and SNU5ranged from 14.6 to 16.8%. However, for SNU216, SNU484, SNU601, SNU638, SNU668, SNU16, and SNU520,they were below10%. These results demonstrate that the FSM extract can be used in the functional food, pharmaceutical,and cosmetic industries.