BRCA1 Reversion Mutation Confers Resistance to Olaparib and Camrelizumab in a Patient with Breast Cancer Liver Metastasis

Jia-Ni Pan,Lei Lei,Wei-Wu Ye,Xiao-Jia Wang,Wen-Ming Cao 한국유방암학회 2021 Journal of breast cancer Vol.24 No.5

Reversion mutations are associated with clinical resistance to poly(ADP-ribose) polymerase inhibitors (PARPi). Here, we describe the detection of a BRCA1 reversion mutation in a 39-year-old woman with metastatic breast cancer harboring a heterozygous germline BRCA1 exons 7–8 deletion who received PARPi olaparib combined with immune checkpoint inhibitor camrelizumab as third-line therapy. During progression from the olaparib and camrelizumab combination therapy, we identified via genomic sequencing a novel 7-base pair somatic deletion in BRCA1 (c.617_623delACAAATC). Sequence analyses indicated that this mutation realigned the reading frame of BRCA1, which potentially led to the reversal of its normal function and conferred resistance to PARPi.

Serum Amyloid A is a Novel Prognostic Biomarker in Hepatocellular Carcinoma

Ni, Xiao-Chun,Yi, Yong,Fu, Yi-Peng,He, Hong-Wei,Cai, Xiao-Yan,Wang, Jia-Xing,Zhou, Jian,Fan, Jia,Qiu, Shuang-Jian Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Purpose: To investigate the prognostic value of serum amyloid A (SAA) in patients with hepatocellular carcinoma (HCC) undergoing surgery. Materials and Methods: Preoperative serum samples of 328 patients with HCC who underwent curative resection and of 47 patients with benign liver lesion were assayed. Serum levels of SAA were measured by enzyme-linked immunosorbent assay and its correlations with clinicopathological characteristics and survival were explored. Results: Levels of SAA were significantly higher in patients with HCC than those with benign liver lesion. There were strong correlations between preoperative serum SAA level and tumor size and more advanced BCLC stage. On univariate analysis, elevated SAA was associated with reduced disease-free survival and overall survival (p=0.001 and 0.03, respectively). Multivariate analyses showed that serum SAA level was an independent prognostic factor for overall survival (hazard ratio 2.80, p=0.01). Conclusions: High SAA serum level is a novel biomarker for the prognosis of HCC patients.

Treatment of Human Thyroid Carcinoma Cells with the G47delta Oncolytic Herpes Simplex Virus

Wang, Jia-Ni,Xu, Li-Hua,Zeng, Wei-Gen,Hu, Pan,Rabkin, Samuel D.,Liu, Ren-Rin Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.3

Background: Thyroid carcinoma is the most common malignancy of the endocrine organs. Although the majority of thyroid cancer patients experience positive outcomes, anaplastic thyroid carcinoma is considered one of the most aggressive malignancies. Current therapeutic regimens do not confer a significant survival benefit, and new therapies are urgently needed. Oncolytic herpes simplex virus (oHSV) may represent a promising therapy for cancer. In the present study, we investigated the therapeutic effects of a third-generation HSV vector, $G47{\Delta}$, on various human thyroid carcinoma cell lines in vitro. Two subcutaneous (s.c.) models of anaplastic thyroid carcinoma were also established to evaluate the in vivo anti-tumor efficacy of $G47{\Delta}$. Materials and Methods: The human thyroid carcinoma cell line ARO, FRO, WRO, and KAT-5, were infected with $G47{\Delta}$ at different multiplicities of infection (MOIs) in vitro. The survival rates of infected cells were calculated each day. Two s.c. tumor models were established using ARO and FRO cells in Balb/c nude mice, which were intratumorally (i.t.) treated with either $G47{\Delta}$ or mock. Tumor volumes and mouse survival times were documented. Results: $G47{\Delta}$ was highly cytotoxic to different types of thyroid carcinomas. For ARO, FRO, and KAT-5, greater than 30% and 80% of cells were killed at MOI=0.01 and MOI=0.1, respectively on day 5. WRO cells displayed modest sensitivity to $G47{\Delta}$, with only 21% and 38% of cells killed. In the s.c. tumor model, both of the anaplastic thyroid carcinoma cell lines (ARO and FRO) were highly sensitive to $G47{\Delta}$; $G47{\Delta}$ significantly inhibited tumor growth and prolonged the survival of mice bearing s.c. ARO and FRO tumors. Conclusions: The oHSV $G47{\Delta}$ can effectively kill different types of human thyroid carcinomas in vitro. $G47{\Delta}$ significantly inhibited growth of anaplastic thyroid carcinoma in vivo and prolonged animal survival. Therefore, $G47{\Delta}$ may hold great promise for thyroid cancer patients.

AR 뉴스의 특성이 수용자의 몰입도 및 정보습득에 미치는 영향 - 중국의 재해, 스포츠 보도를 중심으로

유가니(Jia Ni Liu),이윤(Yoon Lee),이혜은(Hye Eun Lee) 한국콘텐츠학회 2021 한국콘텐츠학회논문지 Vol.21 No.2

증강현실(Augmented Reality: AR)기술은 의료, 교육, 마케팅, 그리고 저널리즘 등 더욱더 다양한 분야에서 활용되고 있다. 본 연구는 2(영상 기술: AR 뉴스, 일반 뉴스) × 2(뉴스 종류: 재난, 스포츠) 피험자 간 실험을 통해 AR 뉴스의 세 가지 특성(시각적 이미지, 스토리텔링, 상호작용성)에 대한 평가가 수용자의 몰입도와 정보습득에 미치는 영향을 알아보고자 한다. 연구 결과, AR 뉴스의 세 가지 특성에 대한 평가가 일반 뉴스에 대한 평가보다 높았다. 또한, 뉴스 종류와 상관없이 AR 뉴스의 스토리텔링과 상호작용성에 대한 평가가 수용자 몰입도에 정적인 영향을 미치는 것으로 나타났지만, 시각적 이미지에 대한 평가는 수용자 몰입도에 유의한 영향을 미치지 않았다. 뉴스 종류와 상관없이 AR 뉴스 시청 후 정보습득이 일반 뉴스보다 높았지만, AR 뉴스의 세 가지 특성에 대한 평가는 정보습득에 영향을 미치지는 않는 것으로 나타났다. 본 연구의 결과들은 AR 뉴스의 향후 발전을 위한 실용적인 전략 수립에 활용될 수 있다는 점에서 시사점을 지닌다. The application of Augmented Reality(AR) technology is visible in medical procedures, education, marketing, and journalism. By experimenting, this study examines how the evaluation of the three elements of augmented reality news contents(visual image, storytelling, interactivity) affects immersion and information acquisition. Specifically, a 2 (video technology: AR vs. general) × 2 (news type: disaster vs. sports) between-subject design was examined. Results showed that the evaluation of all three elements was higher when viewing AR news than when viewing general news. The level of immersion was higher when viewing AR news, and the evaluation of storytelling and interactivity had positive effect on the level of immersion regardless of the news types. However, the evaluation of visual images did not affect the level of immersion. Information acquisition was higher after viewing AR news, yet the effect of the evaluation of the three elements on information acquisition had not been found. Implication and discussion of the study were added in the end.

사실적인 파괴 특수효과의 제작과 360도 파노라마 영상 전시

저우자니(Zhou jia ni),윤태수(Yun tae soo) 한국콘텐츠학회 2018 한국콘텐츠학회 종합학술대회 논문집 Vol.2018 No.5

Effect of light quality on regeneration and naphthoquinones accumulation of Arnebia euchroma

Jia Hu,Yao Leng,Yuanyuan Jiang,Su Ni,Li Zhang 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.4

The influence of light quality on the regeneration (i.e., formation, proliferation, growth, development, and rooting) and naphthoquinones’ accumulation of Arnebia euchroma was investigated in vitro. The cotyledons of sterile seedings were placed on modified LS medium supplemented with 0.8 mg/L thidiazuron to obtain callus, which was incubated on the corresponding medium at different culture stages under varying light conditions, including dark, fluorescent lamp (Fl), white light-emitting diodes (LEDs), blue LEDs, red LEDs, and a combination of 25% red, 25% blue, and 50% white LEDs [RBW (1:1:2)]. During the establishment of the A. euchroma regeneration system, we obtained the maximum fresh weight, dry weight, germination rate, shoot number, and proliferation rate under RBW (1:1:2) LEDs in the formation and multiplication phases; the growth index, shoot height, leaf number, and photosynthetic pigment concentration were promoted under red LEDs in the growth and development phase; and the maximum rooting rate (89.29% ± 2.06%) was observed under red LEDs in the rooting phase. To study the effect of light quality on naphthoquinones’ accumulation of A. euchroma, we used the euphylla to achieve adventitious roots, and the highest shikonin (121.77 ± 6.68 μg/g DW) and β′β-dimethylacrylalkannin (619.18 ± 15.71 μg/g DW) contents were detected in the dark culture. The lowest naphthoquinone contents were recorded under Fl in the adventitious roots. Overall, RBW (1:1:2) LEDs enhanced A. enchroma formation and proliferation, while red LEDs promoted the plant’s growth, development, and rooting. The dark condition was beneficial to naphthoquinones’ accumulation, whereas the light condition inhibited the naphthoquinones production of A. enchroma adventitious roots. Determining the suitable light quality for the regeneration and accumulation of naphthoquinones that is conducive to the protection and promotes resource utilization is essential.

Modeling and Tracing Web Content Provenance

Jing Ni,Jia Hao,Xuemei Li,Tong Zhao 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.4

In recent years, research in data provenance has attracted a lot of attention, since it helps to judge the relevance and trustworthiness of the information enclosed in the data. However, many webpages still lack provenance annotation, and this is a main obstacle of tracing the content. In this paper, we propose a model for on-line Web paper variation, based on the W3C PROV Data Model. A semantic similarity clustering method is adopted to determine the relationship within the documents derivation, and feature words variation and the responsible person can be found with the aid of PROV-O. To verify this model, a detailed case study is shown in this paper.

Two new species of the genus Arboridia Zachvatkin from karst area of southwestern China (Hemiptera: Cicadellidae: Typhlocybinae)

Zhang Ni,Jiang Jia,Song Yue-Hua 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.3

Two new species: A. (Arboridia) xiaotungensis sp. nov. and A. (Arboridia) luojiashangensis sp. nov. from Guizhou Province, China are described and illustrated. In addition, the appearances of A. reniformis Song & Li (2013a) and A. anteoculara Song & Li (2013a) are supplementarily described. A key to distinguish all known species of the genus from China is provided. LSID: https://www.zoobank.org/urn:lsid:zoobank.org:pub:3DD14108-ED08-430D-AE8D-CD0 FCC215A4E

F-box Protein Arabidillo-1 Promotes Lateral Root Development by Depressing the Functioning of GA3 in Arabidopsis

Changjun Mu,Ni Chen,Xiaofeng Li,Pengfei Jia,Zhaoyan Wang,Heng Liu 한국식물학회 2010 Journal of Plant Biology Vol.54 No.5

In Arabidopsis, Arabidillo-1 and Arabidillo-2have great sequence homology to Dictyostelium and metazoan β–catenin/Armadillo, which are important to animal and Dictyostelium development. Arabidillo-1 and Arabidillo-2 promote lateral root formation redundantly in Arabidopsis. Here, we showed that gibberellins (GA3) has a greater inhibitory effect on lateral root growth from the null mutant arabidillo-1 than from the wild type, suggesting that the mechanism for Arabidillo-1-regulated modulation of lateral root proliferation is associated with GA3-metabolic or signaling pathways. Our yeast two-hybrid analysis demonstrated that Arabidillo-1 interacts with ASK2 and ASK11, and that ASK2 can bind with the F-box domain of Arabidillo-1. Therefore, Arabidillo-1 is involved in the ubiquitin/26S proteasome-mediated proteolytic pathway. Based on these results, we conclude that Arabidillo-1 can degrade some positive regulator of the GA3 signaling pathway through selective protein degradation of ubiquitin/26S. Moreover, that process is believed to be the mechanism for Arabidillo-1 promotion of lateral root development in Arabidopsis.

Low Counts of γδ T Cells in Peritumoral Liver Tissue are Related to More Frequent Recurrence in Patients with Hepatocellular Carcinoma after Curative Resection

Cai, Xiao-Yan,Wang, Jia-Xing,Yi, Yong,He, Hong-Wei,Ni, Xiao-Chun,Zhou, Jian,Cheng, Yun-Feng,Jin, Jian-Jun,Fan, Jia,Qiu, Shuang-Jian Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Objectives: TCR-gamma-delta+T cells (${\gamma}{\delta}$ T cells) are non-conventional T lymphocytes that can recognize and eradicate tumor cells. Our previous studies showed that infiltration and function of ${\gamma}{\delta}$ T cells were substantially attenuated in hepatocellular carcinoma (HCC). However, their prognostic value was not clarified. Methods: The association between ${\gamma}{\delta}$ T cells and the clinical outcomes was determined by immunohistochemistry (IHC) in a HCC patient cohort (n = 342). Results:Immunohistochemistry showed decreased infiltration of ${\gamma}{\delta}$ T cells in tumoral tissues compared with paired peritumoral tissues. The counts of ${\gamma}{\delta}$ T cells in peritumoral tissues were negatively correlated with tumor size (P = 0.005). Survival analysis showed that the levels of peritumoral ${\gamma}{\delta}$T cells were related to both time to recurrence (TTR) and overall survival (OS) (P = 0.010 and P = 0.036, respectively) in univariate analysis, and related to TTR in multivariate analysis (P = 0.014, H.R. [95% CI] = 0.682 [0.502-0.927]). Furthermore, the level of peritumoral ${\gamma}{\delta}$ T cells showed independent prognostic value for TTR in Barcelona Clinic Liver Cancer (BCLC) stage A patients (P = 0.038, H.R. [95% CI] = 0.727 [0.537-0.984]). However, tumoral ${\gamma}{\delta}$ T cells did not show independent prognostic value for either TTR or OS in HCC patients. Conclusions: Low counts of ${\gamma}{\delta}$ T cells in peritumoral liver tissue are related to a higher incidence of recurrence in HCC and can predict postoperative recurrence, especially in those with early-stage HCC.