MicroRNA 452 regulates ASB8, NOL8, and CDR2 expression in colorectal cancer cells

Ji‑Su Mo,Soo‑Cheon Chae 한국유전학회 2021 Genes & Genomics Vol.43 No.1

Background MicroRNAs play important roles in the pathogenesis of human diseases by regulating target gene expression in specifc cells or tissues. Previously, we identifed microRNA 452 (MIR452), which was specifcally up-regulated in early stage human colorectal cancer (CRC) tissue. Objective The current study aims to identify and verify the target genes of MIR452 associated with CRC. Methods A luciferase reporter system was used to confrm the efect of MIR452 on ASB8, NOL8, and CDR2 expression. The expression levels of MIR452 and the target genes were evaluated by quantitative RT-PCR (qRT-PCR) and western blotting. Results We verifed the association between MIR452 and three genes, ASB8, NOL8, and CDR2, and showed that their transcripts were down-regulated by MIR452. Up-regulated MIR452 also down-regulated ASB8, NOL8, and CDR2 mRNA and protein levels in CRC cells. CDR2 protein expression was decreased in CRC tissues compared to adjacent non-tumor tissues. Conclusions These results suggest that ASB8, NOL8, and CDR2 were target genes of MIR452 in CRC cells and that upregulated MIR452 in CRC tissue regulated ASB8, NOL8, and CDR2 expression during colorectal carcinogenesis.

Newcastle Disease Virus의 Fusion 단백 유전자의 제한효소분절형태(RELP)의 특이성

장경수,김지영,김석,김태용,송영환,허지연,김현수,송희종,전무형 충남대학교 수의과대학 동물의과학연구소 2001 動物醫科學硏究誌 Vol.9 No.-

To evaluate the specificity of restriction fragment length polymorphism (RFLP) of Newcastle disease virus (NDV) fusion (F) protein gene for various NDV strains. F protein genes of NDV CBP-1 isolate, LaSota, B1 and Kyojungwon (KJW) were amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) and RFLP were examined. In RT-PCR using F primer sets for 1.7 kb F gene, the amplified products were observed in CBP-1, LaSota and B1 except KJW. However, F2 primer set for 521 bp yielded the amplified bands with all strains. The amplified F genes (1.7 kbp) were treatd with 15 different restriction enzymes to analyze RFLP and the cleavage patterns. CBP-1 showed various cleavage patterns with 8 enzymes, LaSota, with 11 enzymes and B1, with 11 enzymes. According to the cleavage patterns, the physical maps were constructed, and it was found that CBP-1, LaSota and B1 strains had specific cleavage sites with HinfI, XbaI and XhoI. These findings suggest that RFLP are worth applying in the diagnosis and molecular epidemiological studies of Newcastle disease.

捻轉法이 竝行된 鍼刺戟 및 電鍼刺戟이 鎭痛效果에 미치는 影響

Park, Dong-Seok,Kim, Ji-Hoon,Min, Byung-Il,Oh, Soo-Myung 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Dong-Seok Park¹, Ji-Hoon Kim¹ and Byung-Il Min, Soo-Myung Oh,³¹Department of Acupuncture and Moxibustion, College of Medicine, ²Department of Physiology, College of Medicine, And ³East-West Medical Research Institute, Kyung Hee University,Seoul, Korea. Analgesic Effect of Manual Acupuncture and Electroacupuncture combined with Twirling Method. Proceeding of International Symposium on East-West Medicine Seoul. 187-200, 1999.-Twirling the needle (TN) is one of several needing methods frequently used for acupuncture in the oriental medicine. The present study was conducted to see if TN enhanced the antinociceptive effect produced by plain-or electroacupuncture in the rat tail flick test. Rats (Sprague-Dawley, 200-300g)lightly anesthetized with thiopental sodium(40mg/kg, i.p.) were used in the tail-flick test. The basal reaction time for tail-flick was 2 sec. For "plain" acupuncture(PA), a needle was inserted for 20 min into a Chock-samni (ST36) acupuncture point which (is located at the anterior tibial muscle and about 10mm below the knee joint). For electroacupuncture(EA), train-pulses(3 Hz,0.3-ms pulse width, 0.2-0.3mA) were applied to the inserted needle for the period of insertion. For combining TN with either PA or EA, TN was performed during the each acupuncture period. Two methods for TN were used; twirling 3 times for 1 second persisted for 1 min in every 5 mins(long-duration and long-interval;LDLI) and twirling 3 times for 1 second persisted for 10 sec in every 1 min(short-duration and short-interval;SDSI). PA and EA produced an increase in tail flick latency (TFL); peak increases were 21.3±5.1% and 42.1±17.3% of the pre-acupunture control, respectively, and occurred immediately after cessation of 20min-acupuncture. Performing TN combined with PA increased TFL more than PA itself by showing a greater peak increase in TFL when performed with a SDSI-TN than with a LDLI-TN(60.6±12.5% and 42.7±22.7% of the pre-acupuncture control, respectively). Performing TN combined with EA also increased TFL more than EA itself and a greater peak increase in TFL was observed with a SDSI-TN as compared to a LDLI-TN(77.5±13.8% and 67.3±14.0% of the pre-acupuncture control, respectively). These results indicate that TN enhances both PA-and EA-produced antinociception where an greater enhanced antinociception than a LDLI-TN. It is suggested that performing a SDSI-TN combined with EA seems to be one of the most effective ways to produce analgesia.

기술수준에 따른 여자축구 인프론트 킥 동작의 협응구조

지보경,육동원,김지수,신정택 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.2

The purpose of this study was to investigate coordination structure of infront kick in women soccer as a function of skill level. To succeed this purpose, four girls' high school students who had never experienced soccer before were selected as novices, and four girls' high school students who had played soccer for over three years as experts in this lab. To recognize the change of coordination structure according to practice, novices had practiced infront kick in soccer for two weeks. consequently they were taken films of kicking motion three times -before practice, after one week practice, after two weeks practice. However, experts were taken once. The experimental instruments for this study consisted of a video camera, reference frame. LED and APAS for digitizing. Angle to angle diagrams and phase plan portraits for qualitative analysis of coordination structure were gotten with LabVIEW 6i. The conclusions are as follow: firstly, novices' coordination structure had been changed since practice. Secondly, there were significantly different between coordination structures of experts and pre-practice novices. Lastly, there were similar between coordination structures of experts and post practice novices.

대칭형 복사기 줌 렌즈계의 광학적 성능 분석

지택상 ; 박수봉 ; 김봉환 김천대학교 2001 김천대학교 논문집 Vol.22 No.-

We analyzed to calculate the optical capacity, after we chose the designed symmetric 3 group zoom copier lens system in this paper.

아시아 경제위기와 외환위기의 원인과 그 대응에 관한 평가

류지수 영남대학교 사회과학연구소 2001 社會科學硏究 Vol.21 No.1

본 논문은 1997년에 발생한 아시아 경제위기에 대해서 고찰하였다. 한국이 IMF의 구제 금융을 받게된 것을 계기로 IMF는 아시아 경제위기의 치유에 주도적인 역할을 맡게 되었다. 그러나 IMF의 구제금융은 아시아의 경제위기를 해결하지 못하였다. 구제금융의 지원에도 불구하고 이들 3개국의 신용등급은 더욱 멀어졌고 통화가치는 빨리 떨어져 외채위기는 더욱 증폭되어 IMF의 목표가 완전히 실패로 돌아갔다.

대기오염이 기관지 천식의 악화에 미치는 영향

류지원,어수택,김기업,이영목,김양기,홍성아,전창균 순천향의학연구소 2007 Journal of Soonchunhyang Medical Science Vol.13 No.1

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유산소성 운동이 비만 남고생의 전신지구력 향상에 미치는 영향

지수찬,김세환 강원대학교 체육과학연구소 2001 江原大學校附設體育科學硏究所論文集 Vol.- No.24

While measuring 1000m running record of high school students, I witnessed that corpulent students feel harder than ordinary students do while running and confirmed that the 1000m running record of the corpulent students is poor as compared with that of ordinary students. I felt a sense of mission that it is required to take measures for the corpulent students, and conducted this study under the understanding of the corpulent students themselves, their class teachers and parents. The conclusion of this study is as follows: 1)The corpulent students who intend to do physical exercise but really carry out their intention have successfully participated in this program as the instruction programs that consider their life style, physical conditions and preference of physical exercise have been effectively implemented including relatively small mountain climbing. 2)Amusing games have been offered to the corpulent students so that they are accustomed to do warming-up and finishing exercise as well as reinforcing exercise form time to time. And relatively over-corpulent students could improve their physical endurance by climbing mountain in a systematic manner.

다 파장 광원 개발을 위한 온도 안정화 특성에 관한 연구

지유강,박수봉 동신대학교 2006 論文集 Vol.16 No.-

본 논문은 광 전송 시스템에서 정보를 전송하기 위하여 필요한 안정된 광원을 얻기 위한 것으로써, 광원을 얻기 위한 광원시스템은 광원 모듈부와 디지털 제어부 그리고 온도 안정화부로 구성하였다. 본 논문에서는 레이져 다이오드의 광 출력을 안정화 시키기 위하여 온도센서와 자체개발한 TEC(Thermo Electric Cooler)를 이용하여 온도제어회로를 구성함으로써 안정된 광원을 얻을 수 있었다. 제안된 온도 안정화부의 온도범위는 ±0.5도 이내에서 제어가 가능하며 이와 같이 구성된 광원시스템의 4파장 광원은 ±0.05dB 이내에서 안정화되었다. In this paper, we composed optical source system of optical module section, digital control section, and temperature stability section to acquire optical source and to transmit information in optical transmit system, we could obtaion optical source stabilized as composed of temperature control circuit to take advantage of temperature sensor and ourselves developed TEC (Thermo Electric Cooler) to balance optical output power of laser diode. The temperature range of proposed temperature stability section make control possible within ±0.5 degree, also four wavelength optical source stabilized less than ±0.05dB.

열대 지역에서 통일형 초다수성 벼 품종의 생육 및 수량성 비교

이지윤,준현,성환,손영보,송유천,한상익,박동수,권영업,이철원,이종희 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2