Multiplex PCR Detection for 3 Events of Genetically Modified Maize, DAS-59122-7, TC6275, and MIR604

Ji-Hye Ahn,Jae-Hwan Kim,Su-Youn Kim,Woo-Young Lee,Sun-Hee Park,Hae-Yeong Kim 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.3

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 3 events of genetically modified (GM) maize. The event-specific primers were used to discriminate the following 3 events of GM maize (DAS-59122-7, TC6275, and MIR604) using multiplex PCR method. The zein gene was used as an endogenous maize reference gene in the multiplex PCR detection. The primer pair Zein-F/R producing a 99 bp amplicon was used to amplify the zein gene. The primer JI-Das-F1/R1 for DAS-59122-7, JI-TC6275-F3/R3 for TC6275, and JI-MIR F1/R1 for MIR604 yielded an amplicon of 130, 162, and 197 bp, respectively. The detection limit of multiplex PCR was 1% for DAS-59122-7, TC6275, and MIR604 for one reaction.

석면함유 슬레이트 지붕 물받이 퇴적물 중 석면 섬유 함유율

임지현,한솔민,김현석,신유민,박시은,허정윤,김민영,장봉기 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

This study attempted to determine the degree of asbestos release from the aging slate roof by comparing the asbestos content in the slate roof rain gutter with a colored steel plate (tin plate) over the slate roof. Four slate roof houses located in Haengmok-ri, Asan-si, Chungcheongnam-do, and one house constructed with a colored steel plate on the slate roof were selected to collect the sediment of the roof rain gutter. The asbestos fiber content was calculated by a point counting method using a polarization microscope after pretreatment with conversion treatment and hydrochloric acid treatment. The average asbestos content of the four slate roof rain gutter were 1.89%. However, asbestos was not detected in the Slate covering roof rain gutter, which were constructed on the slate roof. Asbestos fiber content was the highest at 2.89% in the slate roof rain gutter installed in 1976, followed by 2.44% in 1953. From the above results, it is necessary to minimize secondary damage as asbestos fibers released from slate roof houses to the surrounding atmosphere or leaked from slate roofs as rainwater may cause soil pollution and seriously affect residents' health. Although covering with colored steel plates (tin plates) has been shown to prevent the leakage of asbestos fibers to some extent, it is believed that a policy alternative to remove the slate roof as soon as possible is needed to solve the fundamental problem.

전신홍반루푸스 환자에서 Corticosteroids 복용 중에 B형 간염이 활성화한 1예

곽지영 외 중앙대학교 의과대학 의과학연구소 2011 中央醫大誌 Vol.36 No.3/4

Hepatitis B virus (HBV) infection is endemic in Asia and reactivation of HBV is a well recognized complication in infected patients who undergo cytotoxic chemotherapy for cancer and immunosuppressive therapy. Since immunosuppressant can facilitate viral replication and lead to reactivation of HBV, the management of systemic lupus erythematosus (SLE) patients who are HBsAg-positive presents a clinical challenge. We report here on a 29-year old female with SLE and HBsAg positivity who presented hepatitic flare during corticosteroid treatment only. HBsAg carriers who receive cytotoxic or immunosuppressive therapy are at considerable risk of reactivation of HBV infection. Antiviral prophylaxis is clinically efficacious as primary prophylaxis to prevent HBV reactivation. Risk factors allowing patient stratification for HBV reactivation include foremost the HBV DNA level before initiation of immunosuppresive treatment, including corticosteroids. Monitoring for development of acute flare of HBV should be performed by serial measurement of HBV DNA and liver enzymes regularly.

여수국가산업단지 인근 지역 대기오염물질 농도에 영향을 미치는 요인분석

김민영,변정훈,이영훈,이건영,한지은,이예림,임지현,장봉기 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

Eight monitoring stations in the Yeosu National Industrial Complex will determine the concentration of air pollutants measured every hour in 2019, analyze what factors affect them, and how they correlate with the air pollutant measurements. Six air pollutants (SO2, NO2, O3, CO, PM-10 and PM-2.5) measured at eight monitoring stations around Yeosu National Industrial Complex (Seogang-dong, Wolnae-dong, Munsu-dong, Yeocheon-dong, Deokchung-dong, Hwayang-myeon, Yulchon-myeon, Samil-dong) the measurement data for each hour of the year were downloaded to analyze the concentration by weather condition, time zone, season, separation distance from Yeosu Industrial Complex, and separation distance from Gwangyang Industrial Complex. SO2, NO2, and CO concentrations were significantly higher in winter, significantly lower in summer, and O3, PM-10, and PM-2.5 concentrations were significantly higher in spring. SO2, CO, PM-10, and PM-2.5 concentrations were significantly higher between 10 a.m. and 12 p.m., NO2 concentrations were significantly higher between 7 a.m. and 9 a.m., and O3 concentrations were significantly higher between 13 p.m. and 15 p.m.(p<0.001). The concentration of air pollutants (SO2, NO2, CO, PM-10, PM-2.5) excluding O3 was found to have a negative (-) correlation with weather condition, and in the case of O3, it was found to have a positive (+) correlation with PM-10, PM-2.5, temperature and wind speed. It was confirmed that the concentration of air pollutants emitted from the Yeosu National Industrial Complex differs depending on the season, time and distance to the industrial complex. This study aims to establish measures to minimize health damage to residents living relatively close to the industrial complex and contribute to the establishment of management and response measures according to the properties of substances to reduce the concentration of air pollutants in the future.

근피로 후 세 가지 회복 방법

이예영,김지은,서예은,정다연,강현주 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

Muscle fatigue is a symptom that decreases your muscles’ ability to perform over time. It can be associated with a state of exhaustion, often following excessive sports activity or exercise. While exercise is a common cause of muscle fatigue, this symptom can be the result of other health conditions. Muscle fatigue caused by excessive activity limits activities of daily living and difficult to maintain a stable posture. Therefore, the purpose of this study was to investigate the effect of stretching, icing, and sports massage on muscle fatigue recovery.

영상처리에 의한 버스전용차로 위반차량의 검출방법

김지영 서원대학교 응용과학연구소 2001 응용과학연구 Vol.10 No.1

In this paper, an image processing method detecting the vehicles which violate the exclusive bus lain traffic regulation is proposed. The tow cameras are used to capture the images of the first lain of a highway where the exclusive bus lain traffic regulation is enforced. One camera is used to capture the front view of a vehicle, and the second camera to capture the side view of the craft. The front view camera is used for recognizing the vehicle licence number. The side view camera is employed for categorizing the vehicle class. The proposed method in this paper is very practical to be implemented because it does not need any attachment on the vehicle such as an identification transmitter or any other electronic devices.

긴장증의 임상적 특성에 대한 고찰

송지영,윤도준,변재영,장환일 大韓神經精神醫學會 1989 신경정신의학 Vol.28 No.3

26 cases(9 males and 17 females) with catatonia features on admission were analyzed in terms of its clinical characteristics. The results obtained were as follows : 1) In the diagnostic distribution, more than a half of the cases were schizophrenic disorders(57.5%) and the others were major depressions(26.9%) and schizophreniform disorders(15.4%). 2) The catatonia signs were nonspecific and evenly distributed among a variety of clinical diagnostic entities. But the signs of negativism, mutism, and posturing appeared frequently in all cases. 3) Acute onset was prevalent(65.4%), ECT was more frequently applied(61.5%) and treatment response was better(70.9%) in the catatonia group than non-catatonia. Although the definition of catatonia is not clear yet, above results support the opinions of previous studies that catatonia should be understood as a heterogenous symptom complex. And not only schizophrenia but also affective disorder are associated with catatonia features. This suggests that we have to re-evaluate the concept of Kahlbaum's old description.

급성 뇌졸중 환자에 있어서 병원 도착까지 지연시간 및 지연요인 분석

류지영,어은경,김용재,정구영 대한응급의학회 2000 대한응급의학회지 Vol.11 No.3

Background: Recent advances have been made in the treatment of acute stroke, but the effectiveness of the new therapies is highly time-dependent. The purpose of this study is to investigate the factors that influence the time from symptom onset to hospital arrival and the total arrival delay time for patients with acute stroke. Methods: A prospective registry of patients presenting to the ED with signs or symptoms of acute stroke was established at Ewha Womans university Mokdong and Dongdaemun hospitals from March to December 1999, We analyzed the the prehospital delay time(reaction interval and total arrival delay) and factors associated with delayed arrival at the hospital. Results: The study included 256 patients(49% were women) with a mean age of 62±13 years. 50.9% of the patients arrived within 3 hours, and 94.9% patients arrived within 24 hours after onset of symptoms of acute stroke. The total arrival delay mine was 180 minutes(median time), and the reaction interval was 60 minutes(median time). Transportation by 119 or 129 ambulance was linked to shorter delay(47 minutes). Age, mental status, and degree of disability were statistically significant factors associated with delayed arrival at the hospital. Conclusion: Age, mental status, and degree of disability were significant factors associated with delayed arrival at the hospital. For effective treatment of acute stroke patients, increased public awareness to use an ambulance with direct transport to the acute-care hospital is required.

YCbCr 공간에서의 컬러 영역화

김지영 서원대학교 미래창조연구소 2004 과학과 문화 Vol.1 No.1

In this paper a new color segmentation algorithm is proposed. The proposed algorithm uses YCbCr 3D color space where similar group of colors are easily identified. Two dimensional color space has difficulty in identifying group of colors while the processing time is surely advantageous. In the YCbCr histogram, those groups of colors are looked like stems and branches of a tree. A recursive region growing algorithm is developed and implemented in this paper. The proposed algorithm gives good results but the processing cost is very high. The spatial information of the image plane for the proposed region growing algorithm will be useful for the future research.

한국과 일본의 미술과 교육과정 비교 분석

류지영 춘천교육대학교 초등교육연구소 2004 敎育硏究 Vol.22 No.-

This research aims at considering the present condition of art education and the view in the present public curriculum mainly by the Republic of Korea and Japan. The art education in the Republic of Korea and Japan which have shown deployment different after the war has greeted the new aspect of affairs through this revision of both countries. The following three points can be got as a view of future Art education. 1. Expansion of Comprehensive Education, and Educational Quality 2. It is Serious Consideration of Teacher's Creativity with Child's Creativity. 3. Arts Education Which Gropes for Educational Contents and Method according to Order of Arts As mentioned above, the view has been considered to be the present condition of the appreciation education in a South Korean new curriculum. In spite of having shared the same curriculum as modernization, the art education in the Republic of Korea and Japan which have shown a different aspect has greeted the aspect of affairs new now in the present age. In the increase in the image which will be developed from now on, and the information society of the future which will become more complicated, art education aims at correspondence how, or the department education of art of an elementary school specifies the character how in such a situation, and a role of school education can be played, or the argument about the possibility of a future change and a subject are required.