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Ethionine의 시간투여로 인한 Rat의 간과 신장의 지질과산화와 지질량의 변화 연구
김은석,김현아,이주연,이주현,한정희 曉星女子大學校 藥學大學 學生會 1990 曉星藥誌 Vol.5 No.-
The present studies were undertaken to evaluate the effect of Schedule administration of ethionine on the change of lipid peroxidation, total lipid, phospholipid, total cholesterol, and triglycerides of liver and kidney in male rats. The result obtained were summarized as follows ; 1) Hepatic and renal lipid peroxidation were significantly increased 18hr and 24hr after ethionine I.P. injecion. The change of hepatic and renal lipid peroxidation was maximal 18hr after ethionine I.P. injection. 2) Hepatic total lipid and triglycerides were significantly increased 12hr, 18hr, and 24hr after ethionine I.P. injection. The accumulation of total lipid and triglycerides was maximal 18hr after ethionine I.P. injection.
Microsatellite marker development for onion genetic purity test
Srigopalram Srisesharam,Jeong-Suk Hyeon Han,Ranjith Kumar Manoharan,Ill-Sup Nou 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Bulb onion (Allium cepa), which belongs to the family Amaryllidaceae, is one of the oldest vegetative crops known to humans. Despite its high economic value, only a few reports are available on the use of molecular markers in genetic diversity analysis of Allium cepa for its improvement. Molecular genetic markers have been widely used as powerful tools for analyzing the plant genome. In particular, Microsatellites or simple sequence repeats (SSRs) markers are tandem repeats of one to six bp in length and have been proven to be the most powerful polymerase chain reaction (PCR)-based DNA markers in plant diversity analysis. In this study, the genomic DNA was isolated from different Allium cepa lines. The ESTs and gDNA sequences of onion were collected from National Center for Biotechnology information. The SSRs with two to five motifs over a length of 12 bp, were identified using SSRIT (Gramene) software. The PCR products of 100 to 350 bp in length containing SSRs, primers was designed using Primer3 with lengths of 20 to 24 bp and a melting temperature of 60℃. The SSR markers with high polymorphism-information content (PIC) levels was useful for collecting progeny with high genetic homogeneity for onion breeding, and to obtain representative marker sets for genetic tests. The SSR Finder program and the developed SSR markers could be a useful resource for genetic diversity and purity testing in onion.
Ranjith Kumar Manoharan,Jeong Suk Hyeon Han,Senthil Kumar Thamilarasan,Jong-In Park,Ill-Sup Nou 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Onion and other Allium vegetables have been valued since antiquity for their pungent flavor and aroma. Modern science has confirmed traditional benefits that the organosulfur compounds that impart flavor also confer significant human health benefits such as reduced blood clotting and antimicrobial properties. Glucose, fructose and sucrose comprises majority of onion bulb dry matter content. The sugars, pyruvic acid accumulation and transcript level of some transcription factors involved in the biosynthesis of high sugars and pyruvic acid. These profiles were compared with two different lines 36101 (early) and 36122(Late) of bulb onion (Allium cepa L.) growing under drought and photoperiod condition using High Performance Liquid Chromatography (HPLC) and Quantitative real time PCR using FT genes. We identified the gene AcFT4 was responsible for early and late bulb intiation in the onion lines. The cultivar lines 36101and 36122 were used to identify potential genes controlling pungency and sugar. The comparative analysis of two lines showed significant positive phenotypic and genetic correlations. Sugar and pungency profile showed significant difference between two lines. FT gene expression and pungency level was high in onion lines during drought stress. In this study, we proposed the biochemical characterization of two line and genes involved in the bulb formation were also studied. There is a correlation between sugars and pungency level during the drought stress. These results could be presumably used as useful information to obtain onion varieties rich in sugars and pungency.
Ranjith Kumar Manoharan,Jeong-Suk Hyeon Han,Srigopalram Srisesharam,Mi-Young Chung,Ill-Sup Nou 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Onion is one of the most widely consumed vegetables. There are many cultivars, which are grouped according to skin color as yellow, white or red. Onions can also be classified as sweet or non-sweet. Their importance in cooking comes from their typical taste and flavour. The sugars, pyruvic acid accumulation and transcript level of some transcription factors involved in the biosynthesis of high sugars and pyruvic acid was analyzed at different stages of bulb onion (Allium cepa) growing under light and dark condition using High Performance Liquid Chromatography (HPLC) and Quantitative real time PCR. A genetic map and cultivar lines 36101and 36122 were used to identify transcription factors controlling pungency and sugar. We compared 2 different lines for low pungency and high sugars during water and photoperiod stress, which showed significant positive phenotypic and genetic correlations. These results could be presumably used as useful information to obtain onion varieties rich in sugars.
Senthil Kumar Thamilarasan,Jeong Suk Hyeon Han,Jong-In Park,Ill-sup Nou 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Bulb onion (Allium cepa) is one of the second most widely cultivated and consumed vegetable crops in the world. During winter where the temperature can be as low, plant could get cold injury and limit the production of bulb onion. However, the genomic resources available for bulb onion are still very limited. To date, no studies about heritably durable cold and freezing tolerance were carried out in bulb onion genotypes using high-throughput sequencing technology was applied. We sequenced cold (2°C) freezing (-5 and -15°C) treated and control (25°C) samples of contrasting genotypes of A. cepa lines and obtained 4,52,194,370 total high quality reads. After de novo assembly reads were assembled into 54,047 genes finally generated with an average length of 1,331 bp. Based on the similarity search aligning all genes with known public non-redundant (NR) database, including Swiss-prot, KEGG and COG. Differentially expressed genes (DEGs) were investigated using FPKM method. Overall, 92,862 genes were differentially regulated in all libraries were identified. Additionally, increase our understanding of the DEGs, we performed GO and KEGG pathway enrichment analyses. Based on FDR<=0.01 value in cold freezing tolerant line candidate genes were selected and discussed. Finally 25 candidate genes were examined using qRT-PCR were differentially regulated and known to be associated with cold and freezing stresses. Moreover, in silico prediction of putative molecular marker 4,437 SSRs and 6,076 SNPs. Our study is the first to provide the transcriptome sequence resource of Allium spp., for cold and freezing stress. We identified large set of genes to determine its DEGs profile under cold and freezing condition using two different genotypes. These data provides a valuable resource of genetic and genomic studies of Allium spp.
Han, Joong Tark,Kim, Bo Gyeong,Yang, Myung,Kim, Jun Suk,Jeong, Hee Jin,Jeong, Seung Yol,Hong, Seong-Hyeon,Lee, Geon-Woong American Chemical Society 2011 ACS APPLIED MATERIALS & INTERFACES Vol.3 No.7
<P>We report a facile chemical route for stabilizing a dispersion of carboxylated single-walled carbon nanotubes (SWCNTs) in a ZnO sol. The dispersion is stabilized via capping of the carboxyl groups on the SWCNT surface by a titania layer, which was confirmed by Fourier transform infrared spectroscopy and transmission electron microscopy. We also demonstrate that the conductivity of the films prepared from the SWCNT/TiO<SUB><I>x</I></SUB>/ZnO sol is dramatically enhanced by thermal treatment and that the thermal stability of the hybridized films with the ZnO sol is notably improved relative to that of a pristine SWCNT film. The structural and chemical changes of the fabricated films were characterized by Raman spectroscopy. As one application, it was presented that thermally treated SWCNT/TiO<SUB><I>x</I></SUB>/ZnO hybrid thin film sensors showed hydrogen sensing characteristics even at room temperature.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2011/aamick.2011.3.issue-7/am200450u/production/images/medium/am-2011-00450u_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am200450u'>ACS Electronic Supporting Info</A></P>