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곽정훈,최동윤,박치호,김재환,정광화,양창범,유용희,천현식,라창식,Kwag, J.H.,Choi, D.Y.,Park, Ch.H.,Kim, J.H.,Jeong, K.H.,Yang, Ch.B.,Yoo, Y.H.,Chen, H.S.,La, C.S. 한국축산환경학회 2007 축산시설환경학회지 Vol.13 No.3
본시험은 비육돈사료에 미생물제제를 사료에 미생물제제 A 및 B 0.1 미생물제제 C를 0.2% 혼합 급여할 경우 사료섭취량 및 돈분의 오염물질 배설농도에 미치는 영향을 분석하기 위하여 4처리$\times$반복당 5두로서 총 20두를 공시하여 실시하였는데 그 결과를 요약하면 다음과 같다. 1. 비육돈의 일일 평균사료섭취량은 대조구 3.15 kg/일.두였고 미생물A, B, C구는 각각 3.14kg/일/두, 3.31, 3.42로 미생물제제 C구에서 일일 사료섭취량이 가장 높게 조사되었으며(p<0.05), 2. 일일평균 음수량은 사료섭취량이 높았던 미생물 C구에서 3.95kg/일/두로 가장 높게 조사되었다(p<0.05). 3. 미생물제제 처리구별로 분뇨배설량은 사료섭취량이 높았던 미생물제제 C구에서 가장 많이 배설되는 것으로 조사되었으며(p<0.05), 돈뇨의 배설량도 미생물제제 C구에서 2.23kg/일/두에서 높았다(p<0.05). 4. 돈분뇨의 수분 함량은 및 비료성분인 T-N, $P_{2}O_{5}$, $K_{2}O$ 성분도 처리 간에 큰 차이를 보이지 않았다(p<0.05). 5. 돈분뇨의 평균 BOD 농도는 돈분의 경우 미생물제제 B, C제제 급여구가 유의적으로 높게 조사되었다(p<0.05). 그리고 돈뇨의 BOD의 경우에는 대조구에서 $8,657.5mg/{\ell}$로 가장 높은 것으로 조사되었다(p<0.05). 6. COD 농도는 대조구에서 가장 높게 조사되었으며(p<0.05). 돈뇨의 경우에는 미생물제제 A급여구에서 평균 $9,545mg/{\ell}$로 가장 높았다(p<0.05). 7. SS 농도는 미생물제제 B급여구에서 가장 높게 조사되었으며(p<0.05), 돈분뇨중의 T-N 농도는 처리구간에 유의적인 차이가 나타나지 않았다(p<0.05). 그리고 T-P 농도의 경우에는 미생물제제 C급여구에서 유의적인 차이가 나는 것으로 조사되었다(p<0.05). 이상의 결과를 요약해보면 비육돈에 미생물제제 혼합급여시 사료섭취량과 음수량을 증가시키는데 효과가 있는 것으로 조사되었으나, 비료성분 배설량에는 큰 차이를 보이지 않는 것으로 조사되었으나, BOD 등 오염물질농도의 경우에는 미생물제제 A급여구에서 가장 낮게 조사되어 비육돈사료에 미생물제제 급여시 오염물질 저감효과가 있는 것으로 조사되었다. Study for the effect of three different microbial feed additives(henceforth MA-A, MA-B, and MA-C) on feed coversion rate, and physical and chemical characteristics of swine finisher was conducted. MA-B had higher number of Lactobacillus spp. and yeast, compared to any other. The amylase activity of MA-B was also higher than any other. The daily feed intake rates of pigs fed control, MA-A, MA-B and MA-C were 3.15, 3.14, 3.31 and 3.42 kg, respectively. MA-C had the highest weight gain. However, there was no significant difference between treatments. The weights of feces daily excreted by pigs fed control, MA-A, MA-B, and MA-C were 2.14, 2.02, 2.18, and 2.23 kg/day, respectively. The volume of urine daily excreted by pigs fed control, MA-A, MA-B, and MA-C were 3.14, 3.26, 3.27, and $3.41\;{\ell}/day$, respectively. Water content, T-N, $P_{2}O_{5}$, and $K_{2}O$ in swine manure were not significantly different between treatments. The BOD were between 42,576 and $67,450\;mg/{\ell}$ for feces and were between 5,882.5 and $8,657.5\;mg/{\ell}$ for urine, respectively. The SS were between 138,000 and $180,000\;mg/{\ell}$ for feces and were between 875.0 and $1450.0mg/{\ell}$ for urine, respectively.
Kim, B.S.,Jeong, C.S.,Kim, J.M.,Park, S.B.,Park, S.H.,Jeon, J.K.,Jung, S.C.,Kim, S.C.,Park, Y.K. Elsevier Science Publishers 2016 CATALYSIS TODAY - Vol.265 No.-
<P>H-V-MCM-41 catalysts containing 5, 10, and 30 wt% of vanadium were synthesized and applied to the ex situ catalytic pyrolysis (CP) of three polymeric components of lignocellulosic biomass for the first time. Characterization of the catalysts was performed using N-2 adsorption-desorption, XRD, FT-IR, and NH3-TPD. The results of XRD analysis showed that 5 wt% and 10 wt% H-V-MCM-41 catalysts maintained the mesoporous structure, whereas the mesoporous structure was destroyed in 30 wt% H-V-MCM-41 with considerable amount of small V2O5 crystalline outside the framework. NH3-TPD showed that H-V-MCM-41 has mostly weak acid sites and that 10 wt% H-V-MCM-41 had the largest quantity of acid sites due to framework vanadium. In the case of CP of cellulose using Py-GC/MS, 10 wt% H-V-MCM-41 showed the highest catalytic activity for the production of valuable furanic compounds such as furfural because of the enhanced deoxygenation over the acid sites formed on framework vanadium. In the case of CP of xylan as well, 10 wt% H-V-MCM-41 led to the largest yield of mono-aromatics. The production of acetic acid was also promoted by H-V-MCM-41 catalysts. The CP of lignin over H-V-MCM-41 catalysts promoted substantially the production of important feedstock chemicals for the petrochemical industry: phenolics and mono-aromatics. (C) 2015 Elsevier B.V. All rights reserved.</P>
Jeong, J.B.,Chul Hong, S.,Jin Jeong, H. G. Fischer 2009 Phytomedicine Vol.16 No.1
Barley is a major crop worldwide. It has been reported that barley seeds have an effect on scavenging ROS. However, little has been known about the functional role of the barley on the inhibition of DNA damage and apoptosis by ROS. In this study, we purified 3,4-dihydroxybenzaldehyde from the barley with silica gel column chromatography and HPLC and then identified it by GC/MS. And we firstly investigated the inhibitory effects of 3,4-dihydroxybenzaldehyde purified from the barley on oxidative DNA damage and apoptosis induced by H<SUB>2</SUB>O<SUB>2</SUB>, the major mediator of oxidative stress and a potent mutagen. In antioxidant activity assay such as DPPH radical and hydroxyl radical scavenging assay, Fe<SUP>2+</SUP> chelating assay, and intracellular ROS scavenging assay by DCF-DA, 3,4-dihydroxybenzaldehyde was found to scavenge DPPH radical, hydroxyl radical and intracellular ROS. Also it chelated Fe<SUP>2+</SUP>. In in vitro oxidative DNA damage assay and the expression level of phospho-H2A.X, it inhibited oxidative DNA damage and its treatment decreased the expression level of phospho-H2A.X. And in oxidative cell death and apoptosis assay via MTT assay and Hoechst 33342 staining, respectively, the treatment of 3,4-dihydroxybenzaldehyde attenuated H<SUB>2</SUB>O<SUB>2</SUB>-induced cell death and apoptosis. These results suggest that the barley may exert the inhibitory effect on H<SUB>2</SUB>O<SUB>2</SUB>-induced tumor development by blocking H<SUB>2</SUB>O<SUB>2</SUB>-induced oxidative DNA damage, cell death and apoptosis.
최재혁,B. C. Choi,S. M. Lee,S. H. CHUNG,K. S. Jung,W. L. Jeong,S. K. Choi,S. K. Park 대한기계학회 2015 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.29 No.5
In order to investigate the effect of DME mixing on the number density and size of soot particles, DME was mixed in a counter flownon-premixed ethylene flame with mixture ratios of 5%, 14% and 30%. A laser extinction/scattering technique has been adopted tomeasure the volume fraction, number density, and mean size of soot particles. The experimental results showed that the highest soot concentrationswere observed for flames with mixture ratios of 5% and 14%; however, for a mixture ratio of 30% the soot concentrationdecreased. Numerical results showed that the concentrations of propargyl radicals (C3H3) at the 5% and 14% ratios were higher thanthose measured in the ethylene-based flame, and the production of benzene (C6H6) in the 5% and 14% DME mixture flames was alsoincreased. This indicates the crucial role of propargyl in benzene ring formation. These reactions generally become stronger with increasedDME mixing, except for A1 + H2 → A1 + H (-R554) and n-C4H5 + C2H2 → A1 + H (R542). Therefore, it is indicated that addingDME to ethylene flames promotes benzene ring formation. Note that although the maximum C6H6 concentration is largest in the 30%DME mixing flame, the soot volume fraction is smaller than those for the 5% and 14% mixture ratios. This is because the local C6H6concentration decreases in the relatively low temperature region in the fuel side where soot growth occurs.
Paenibacillus pueri sp. nov., isolated from Pu'er tea
Kim, B.-C.,Jeong, W.-J.,Kim, D. Y.,Oh, H.-W.,Kim, H.,Park, D.-S.,Park, H.-M.,Bae, K. S. Microbiology Society 2009 International journal of systematic and evolutiona Vol.59 No.5
<P>Pu'er tea is a fermented drink made from the leaves of the tea plant, Camellia sinensis. Two novel bacteria, designated strains b09i-3(T) and b13i-1, were isolated during the process of fermentation of this tea. These isolates were Gram-positive, endospore-forming, motile rods that grew at 25-42 degrees C and pH 5.5-10.4. The DNA G+C content was 56.6-58.4 mol%, the predominant isoprenoid quinone was MK-7 and the predominant cellular fatty acid was anteiso-C(15 : 0) (49.0-50 % of the total). Phylogenetic analysis based on 16S rRNA gene sequences showed that strains b09i-3(T) and b13i-1 shared 99.9 % similarity and were affiliated with a cluster within the family Paenibacillaceae. Strains b09i-3(T) and b13i-1 were related most closely to Paenibacillus ginsengihumi DCY16(T) (97 % 16S rRNA gene sequence similarity). Levels of DNA-DNA relatedness between the two novel isolates and P. ginsengihumi DCY16(T) were below 56 %. The phylogenetic and phenotypic characteristics of these novel isolates allowed them to be distinguished clearly from recognized species of the genus Paenibacillus. Based on these data, strains b09i-3(T) and b13i-1 are considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus pueri sp. nov. is proposed. The type species is b09i-3(T) (=KCTC 13223(T)=CECT 7360(T)).</P>
Multiple novel H5N6 highly pathogenic avian influenza viruses, South Korea, 2016
Lee, E.K.,Song, B.M.,Lee, Y.N.,Heo, G.B.,Bae, Y.C.,Joh, S.J.,Park, S.C.,Choi, K.S.,Lee, H.J.,Jang, I.,Kang, M.S.,Jeong, O.M.,Choi, B.K.,Lee, S.M.,Jeong, S.C.,Park, B.K.,Lee, H.S.,Lee, Y.J. Elsevier Science 2017 INFECTION GENETICS AND EVOLUTION Vol.51 No.-
<P>We report the identification of novel highly pathogenic avian influenza viruses of subtype H5N6, Glade 23.4.4, that presumably originated from China. In addition, reassortant strains with Eurasian lineage low pathogenic avian influenza viruses were isolated in wild birds and poultry in South Korea. The emergence of these novel H5N6 viruses and their circulation among bird populations are of great concern because of the potential for virus dissemination with intercontinental wild bird migration. (C) 2017 Elsevier B.V. All rights reserved.</P>
Preparation of zwitterion-type chromium(II) complexes for ethylene oligomerization
Lee, D.H.,Kim, E.H.,Jeon, J.Y.,Park, S.H.,Jeong, M.S.,Kang, Y.Y.,Lee, J.,Lee, B.Y. Elsevier Sequoia 2016 Journal of organometallic chemistry Vol.803 No.-
<P>Lithium bis(phosphino)aluminate (4, [Et2Al(PPh2)(2)]Li center dot(THF); 5, [Et2Al(PCy2)(2)]Li center dot(THF)(2)), lithium bis(amino)aluminate (6, [Et2Al(NC4H8)(2)]Li center dot(THF); 7, [Et2Al(NiPr2)(2)]Li; 8, [iBu(2)Al(NiPr2)(2)]Li), and potassium bis(phosphino)borate ligands (11, [(C6F5)(2)B(PPh2)(2)]K; 12, [(C6F5)(2)B(PCy2)(2)]K) were prepared. Reaction of 4-6 with CrCl3(THF)(3) did not provide any discrete chromium complexes, but the reaction of 5-6 with CrCl2(THF) yielded zwitterion-type Cr(II) complexes (13, [Et2Al(PCy2)(2)](2)Cr(II); 14, [Et2Al(NC4H8)(2)](2)Cr(II)). Reaction of THF-free 7-8 with CrCl3(THF)(3) resulted in the formation of Cl-bridged Cr(II) dimer complexes {[Et(Cl)Al(NiPr2)(2)]Cr(II)-mu(2)-Cl}(2) (15) and {[iBu(Cl)Al(NiPr2)(2)]Cr(II)-mu(2)-Cl}(2) (16). A similar Cl-bridged Cr(II) dimer complex {[(C6F5)(2)B(PCy2)(2)]Cr(II)-mu(2)-Cl}(2) (17) was also obtained by reacting the borate ligand 12 with CrCl2(THF). The structures of 13-17 were elucidated by X-ray crystallography. None of the prepared zwitterion-type Cr(II) complexes 13-17 exhibited any activity after activation with MAO, supporting catalytic cycle involving Cr(I/III) oxidation states in ethylene oligomerization. (C) 2015 Elsevier B.V. All rights reserved.</P>