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Hwang, JeeNa,Li, Jinjie,Liu, Wing-Yee,An, Song-Ji,Cho, Hwajin,Her, Nam Han,Yeam, Inhwa,Kim, Dosun,Kang, Byoung-Cheorl Springer-Verlag 2009 Molecules and cells Vol.27 No.3
<P>To evaluate the involvement of translation initiation factors eIF4E and eIFiso4E in Chilli veinai mottle virus (ChiVMV) infection in pepper, we conducted a genetic analysis using a segregating population derived from a cross between Capsicum annuum 'Dempsey' containing an eIF4E mutation (pvr1(2)) and C. annuum 'Perennial' containing an eIFiso4E mutation (pvr6). C. annuum 'Dempsey' was susceptible and C. annuum 'Perennial' was resistant to ChiVMV. All F(1) plants showed resistance, and F(2) individuals segregated in a resistant-susceptible ratio of 166:21, indicating that many resistance loci were involved. Seventy-five F(2) and 329 F(3) plants of 17 families were genotyped with pvr1(2) and pvr6 allele-specific markers, and the genotype data were compared with observed resistance to viral infection. All plants containing homozygous genotypes of both pvr1(2) and pvr6 were resistant to ChiVMV, demonstrating that simultaneous mutations in eIF4E and eIFiso4E confer resistance to ChiVMV in pepper. Genotype analysis of F2 plants revealed that all plants containing homozygous genotypes of both pvr1(2) and pvr6 showed resistance to ChiVMV. In protein-protein interaction experiments, ChiVMV viral genome-linked protein (VPg) interacted with both eIF4E and eIFiso4E. Silencing of eIF4E and eIFiso4E in the VIGS experiment showed reduction in ChiVMV accumulation. These results demonstrated that ChiVMV can use both eIF4E and eIFiso4E for replication, making simultaneous mutations in eIF4E and eIFiso4E necessary to prevent ChiVMV infection in pepper.</P>
황지나(Jeena Hwang),서주환(Ju Hwan Seo),임정선(Jung-Sun Lim),유형선(Hyoung Sun Yoo),박진한(Jinhan Park),김유일(You-eil Kim),김지희(Ji Hui Kim) 한국콘텐츠학회 2017 한국콘텐츠학회논문지 Vol.17 No.7
기업 내부 및 외부 환경 요인 분석을 통한 올바른 비즈니스 전략 수립은 기업의 혁신성과 창출로 이어질 수 있다. 때문에 SWOT 분석을 통해 기업 내부의 강점요인과 약점요인을 인지하고, 기업 외부의 기회요인과 위협요인을 정확하게 분석하는 과정은 매우 중요하다. 그러나 많은 기업들이 그들의 상대적인 강점요인과 약점요인, 기회요인과 위협요인에 대해 정확하게 판단하지 못하는 경우가 많다. 본 연구는 기업이 환경분석 과정에서 보편적으로 활용하는 분석모델 중 하나인 SWOT 분석을 지원하는 도구를 설계 및 구현한 것으로, 각 분야의 전문가들이 작성한 SWOT 분석 결과를 바탕으로 SWOT 분석 사례 DB를 만들어 사용자가 데이터베이스에 저장되어있는 각각의 환경요소를 조회하여 기업에 적합한 환경요소들을 선택, 수정, 추가하게 함으로써 SWOT 분석 시에 기업들이 고려해야하는 내부 및 외부 환경 요인에 대한 정보를 제공하고자 한다. 이를 통해 기업들이 자신의 내부 역량에 대해 보다 정확하게 인지하고, 외부 환경 변화에 대한 고찰을 도울 수 있을 것으로 사료된다. The best business strategy leading to innovation and productivity can be achieved by carefully analyzing internal and external environments of a company. Many companies often require, but difficult to find a tool to determine their own internal/external environmental factors including strengths, weaknesses, opportunities and threats(SWOT). SWOT is one analytical base model that is utilized in this research to design semi-automated environmental analysis process. This study investigates on SWOT generation system that is built on existing analysis database created by experts in each field. Companies can search and choose their best expressing environmental elements that are stored in the database. This semi-automated SWOT tool is expected to contribute that companies can recognize their internal capabilities more accurately, and help consider external environment changes around them.