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Volatile Compositions of Different Ginseng Cultivars under Different Cultivation Years
Yejin Kim,Jeehye Sung,Jung-Woo Lee,Inbae Jang,Ick-Hyun Jo,Nayeong Kwon,Woojong Jang,Dong-Hwi Kim,Kyong-Hwan Bang 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
The volatile compositions of thre Korean ginseng cultivars (yunpoong, gumpoong, and cheonryang) and American ginseng under different cultivation years were analyzed by using HS-SPME/GC-MS. The differential volatile compounds were determined by multivariate statistical analysis including supervised PCA and unsupervised PLS-DA. In the present study, the results showed that major volatile compounds of ginseng cultivars were terpenes, such as spathulenol, β-myrcene, β-pinene, caryophyllene, D-limonen, sabinene and camphene. In particular, hexanal, γ-selinene, octanal, bicyclogermacrene, α-gurjunene, β-sesquiphellandrene, ginsinsene and panaginsene were mainly associated with Korean ginsengs. On the other hand, there was no significant difference between the volatile compounds of ginsengs and different cultivation years. The discrimination between Korean ginsengs and American ginseng could be contribute to β-farnesene, β-panasinsene, α-neoclovene, and α-isocomene. This study concludes that the volatile compositions of ginsengs could be more influenced by cultivars than the cultivation years.
Byungkwan Lee,Minh Duy Pham,Hyunseung Hwang,Inbae Jang,Changhoo Chun 한국원예학회 2021 원예과학기술지 Vol.39 No.2
To design a novel plug tray for ginseng seedling production, the effects of cell height and diameter, which determine root-zone volume, on growth and morphology of ginseng seedlings were investigated. Stratified seeds of the cultivar ‘Chunpoong’ were sown into containers with different cell heights (150, 200, 250, and 300 mm) with a diameter of 50 mm, denoted as H150, H200, H250, and H300, and different cell diameters (15, 20, 30, 50, and 75 mm) with a height of 200 mm, denoted as D15, D20, D30, D50, and D75, and filled with commercial growing media. Seedlings were then grown for 20 weeks in an ebb-and-flow subirrigation system installed in a plant factory with artificial light. Fresh and dry weights, length of roots, and leaf area increased as the cell height increased up to 300 mm. Length, fresh and dry weights of both shoots and roots, root diameter, and leaf area all increased as the cell diameter increased up to 30 mm. The root diameter was not significantly different between D30 and D75, though the roots had more space to expand. A further increase in cell height and diameter beyond H200 or D30 was not effective at increasing plant growth. The roots of ginseng seedlings were long and thick in the cells with a height of 200 mm and diameter of 30 mm. As a result, a novel plug tray was developed with a height and diameter of 200 mm and 30 mm, respectively, resulting in a root volume of 141.37 mL and planting density of 1,156 seedlings/㎡(9 ㎠/plants).
Su Ryun Choi,Sang Heon Oh,Vignesh Dhandapani,Chang Soon Jang,Chun-Hee Ahn,Jana Jeevan Rameneni,Hyuna Kim,Inbae Jeon,Yong Pyo Lim 한국원예학회 2020 Horticulture, Environment, and Biotechnology Vol.61 No.2
Traditional breeding methods usually involve fi eld tests carried out by experienced breeders. However, such methods arecostly and time-consuming. Recently, with the development of Next-Generation Sequencing (NGS) technology, molecularmarkers are being utilized for selection processes in breeding. To implement a high-throughput system using molecularmarkers in Chinese cabbage ( Brassica rapa subsp. pekinensis ) breeding, we developed single nucleotide polymorphism(SNP) marker sets for background selection and testing F 1 purity using Fluidigm genotyping assays. SNPs were generatedusing NGS technology on 209 varieties of Chinese cabbage collected from around the world. Those with minor allele frequency≥ 5% and polymorphism information content ≥ 0.3 were screened, and then based on the physical distribution amongthe 10 chromosomes, 177 SNPs were selected and synthesized for testing. To obtain marker sets with high selection effi ciency,we tested 192 SNPs on 45 types of inbred lines and 29 types of F 1 hybrids. Among the 192 SNPs, we selected 96 markerssets for background selection and 24 marker sets for F 1 purity testing according to the following criteria; the genotype of theparents was homozygous, and the F 1 follows the parents’ genotypes. These SNP sets are suitable for high-throughput systemsusing the 96.96 and 192.24 integrated fl uidic circuit platforms of Fluidigm genotyping assays. These SNP marker sets are notonly effi cient for selecting of early fi xed lines as background selection but are also useful for testing the purity of F 1 hybrids.