http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Changhoo Chun,Haeyoung Na 한국원예학회 2011 원예과학기술지 Vol.29 No.5
Cold pretreatment, washing medium and composition of nutrient media may have marked effects on microspore embryogenesis. When microspores isolated from radish (Raphanus sativus L. cv. Gwanhun) flower buds were washed with Nitsch & Nitsch (NLN) medium liquid medium containing 130 g·L-1 sucrose (NLN-13), yields of microspore-derived embryos were greater than when using B5 liquid medium containing 130 g·L-1 sucrose. Microspore viability is known to decrease rapidly with storage; however, in this experiment, microspore viability was maintained for 24 h at 4°C without media. Among the various medium concentrations used (0.25*, 0.5*, 1.0*, 2.0*, and 4.0* NLN liquid medium), 0.5* NLN liquid medium induced the most efficient formation of microspore-derived embryos. In addition, microspore-derived embryos yields were greater when microspores were cultured in 0.5* NLN liquid medium supplemented with 0.25*, 0.5*, and 1.0* NLN microelements, compared to medium not supplemented with microelements. In this study, the highest yield of microspore-derived embryos was observed when the microspores derived from flower buds were washed using NLN-13 liquid medium and then cultured on 0.5* NLN liquid medium supplemented with 0.25* NLN microelements, followed by incubation at 25°C for 30 days.
Chun, Changhoo,Kozai, Toyoki The Korean Society of Plant Biotechnology 2001 Plant molecular biology and biotechnology research Vol.3 No.2
Photoautotrophic micropropagation systems do not include sugar in the culture media. This characteristic provides advantages to scale up the micropropagation systems comparing photomixotrophic micropropagation systems. A closed, large-scale photoautotrophic micro-propagation for transplant production system has been developed at Chiba University, Japan. New concepts and technologies were adapted to produce high quality transplants at minimum usage of resources, and as scheduled. Newly developed software for production management was used to enhance the efficiency of the transplant production system. Currently, virus-free transplants of sweetpotato (Ipomoea batatas (L.) Lam.) are vegetatively propagated and produced under sterilized conditions in this system. This system can also be used for production of transplants of any other species including horticultural and woody plants with a minimum of modification.