국내 수집 야생버섯류 추출물의 생리활성 비교

안기홍,한재구,김옥태,조재한,An, Gi-Hong,Han, Jae-Gu,Kim, Ok-Tae,Cho, Jae-Han 한국버섯학회 2021 한국버섯학회지 Vol.19 No.1

국내 자생하는 야생버섯 추출물의 생리활성 성분을 평가하기 위하여 각지에서 수집된 야생버섯 70% 에탄올추출물에 대한 DPPH 라디컬 소거능, 총 폴리페놀 및 총 플라보노이드 함량, 철 환원 항산화능, 환원력의 항산화 활성, 아질산염 소거능 및 야생버섯의 건조시료를 이용하여 베타글루칸 함량을 분석하였다. 본 연구에서 수집된 10종의 야생버섯류 중에서 영지(OK1362) 에탄올추출물의 DPPH 라디컬 소거능(73.2%), 총 폴리페놀 함량(28.9 mg GAE/g) 및 총 플라보노이드 함량(10.0 mg QE/g)철 환원 항산화능(0.134), 환원력(0.155), 아질산염 소거능(56.3%)이 다른 버섯류에 비하여 유의적으로 가장 높았다. 그 이외에도 아까시흰구멍버섯(OK1360), 광대버섯속의 A. lanigera (OK1398), 졸각무당버섯(OK1406)이 높은 항산화능 및 아질산염 소거능을 가지고 있음을 확인하였다. 또한 베타글루칸 함량은 영지(OK1362)가 25.2%를 나타내며 가장 높았으며, 그 외에 구름버섯속의 T. lactinea (OK1457)가 24.5%로 높은 것으로 나타났다. 본 연구의 결과는 국내 자생하는 야생버섯류 중 새로운 천연물 유래 생리활성 물질을 탐색하기 위한 기초자료로서 활용 가능성을 기대한다. The aim of the study was to obtain the extracts of various native wild mushrooms and select the useful resources though biological activity evaluation. The anti-oxidant potential, nitrite scavenging activity, and ��-glucan content of wild mushrooms collected from Eumseong and Bonghwa in Korea were investigated. Based on the results of this study, Ganoderma lingzhi (OK1362) showed the highest DPPH radical scavenging activity (73.2%), ferric reducing anti-oxidant power (0.134), reducing power (0.155), nitrite scavenging activity (53.6%), total polyphenol content (28.9 mg GAE/g), flavonoid content (10.0 mg QE/g), and ��-glucan content (25.2%) when compared to other wild mushrooms sampled in this study. In addition, it was confirmed that Perenniporia fraxinea (OK1360), Amanita sp. (OK1398), and Russula sp. (OK1406) had relatively high anti-oxidant and nitrite scavenging potentials. In conclusion, our results can provide fundamental data for extracting beneficial compounds from wild mushrooms.

Progastrin-releasing peptide as a diagnostic and therapeutic biomarker of small cell lung cancer

오형주,( Ha Young Park ),( Tae Ok Kim1 ),( Chul Kyu Park ),( Hong Jun Shin ),( Hee Jung Ban ),( In Jae Oh ),( Yong Soo Kwon ),( Yu Il Kim ),( Sung Chul Lim ),( Young Chul Kim ),( Soo Hyun Kim ),( Myung G 대한결핵 및 호흡기학회 2015 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.120 No.-

Background: Progastrin-releasing peptide (proGRP) is a recently identified biomarker of small cell lung cancer (SCLC). We aimed this study for evaluating the usefulness of automated proGRP measurement for diagnosis and treatment monitoring in patients with SCLC. Methods: From January 2011 to December 2013, plasma samples were prospectively collected from 452 [213 non-small cell lung cancer (NSCLC), 104 SCLC, 135 other diseases] patients visited for tissue diagnosis and tested by two-step automated immunoassay using the ARCHITECT proGRP assay kit (Abbott Diagnostics, USA). The cutoff level of proGRP was set at 63 pg/mL. Results: The mean proGRP was higher in SCLC (1823.0 ± 2684.0 pg/mL) than in NSCLC (61.0 ± 341.7 pg/mL) and other diseases (51.5 ± 222.6 pg/mL, p<0.001). The sensitivity of proGRP was 85.7% (90/105) in SCLC and 11.8% (25/212) in NSCLC. The specificity was 90.2%, positive predictive value was 72.5%, and negative predictive value was 95.4% in SCLC. The mean proGRP was higher in extensive disease (2158.1 ± 2980.6 pg/mL) than in limited disease (901.4 ± 1216.0 pg/mL, p=0.033). Among the 39 patients with SCLC could be followed, the mean proGRP levels of 23 responders were significantly decreased after chemotherapy (from 1651.5 ± 1386.4 pg/mL to 290.0 ± 524.8 pg/mL, p<0.001), whereas those of the 16 non-responders were not. (from 572.5 ± 790.3 pg/mL to 494.4 ± 610.9 pg/mL, p=0.583). Conclusion: Plasma proGRP could be a useful biomarker of SCLC for diagnosis and treatment monitoring. And the initial level may represent the tumor extent of SCLC.

Distribution of Aquaporins (Water Channels) in the Rat Salivary Glands

정지연(Ji-Yeon Jung),한창룡(Chang-Ryoung Han),정연진(Yeon-Jin Jeong),오원만(Won-Mann Oh),김미원(Mi-Won Kim1),김선헌(Sun-Hun Kim),김옥준(Ok-Joon Kim),김현진(Huyn-Jin Kim),고정태(Jeong-Tae Koh),최홍란(Hong-Ran Choi),김원재(Won-Jae Kim) 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.3

침샘은 하루 1.5 l의 침을 분비하며, 일반적으로 침샘에서 수분이동은 능동적 염분 이동에 따른 삼투현상에 의해 일어난다. 따라서 침샘에서 높은 수분투과성으로 인해 수분통로 단백인 aquaporin (AQP)들이 풍부하게 존재할 수 있다. 지금까지침샘에 4가지 형태의 AQP이 존재한다고 알려져 있지만 침샘에서 정확한 위치와 수분 이동에 대한 각 AQP들의 역할은 아직 확실치 않다. 본 연구는 300 g 정도의 흰쥐를 pentobarbital sodium (50 mg/kg, IP) 마취하에서 carbarchol (10 μg/kg)을 복강 내 투여한 후 AQP들의 역할과 분포를 면역조직화학방법으로 조사하였다. AQP1은 침샘의 미세혈관의 내피세포와 샘세포와 샘관 주위에 존재하는 근육상피세포에 존재하였다. AQP4는 침샘관의 마지막 부위인 배출관에 존재하였다. AQP5는 주로 장액세포의 바닥가쪽과 세포사이 분비소관을 포함한 세포꼭대기쪽막 에 존재하였으며 콜린성 분비 자극으로 세포꼭대기쪽막으로 이동되어 밀집되었다. AQP5는 모든 침샘의 사이관과 줄무늬 관에도 존재하였으며 점액세포는 약하게 존재하였다. AQP8은 AQP5처럼 장액세포의 바닥쪽막과 세포사이 분비소관을 포함한 세포꼭대기쪽막에 존재하였으며 콜린성 침분비 자극으로 세포꼭대기쪽막쪽으로 이동되어 밀집되었다. 이상의 실험결과는 AQP5와 AQP8이 장액세포에서 일차 침 형성시 일어나는 수분이동의 주된 통로임을 시사하였다. The salivary glands produce 1.5 l of fluid per day. As in other organs, the general paradigm in the salivary glands is that water movement occurs secondary to osmotic driving forces created by active salt transport. Therefore, high water permeability in salivary glands is expected to need a variety of aquaporin (AQP), a water channel. Although four AQPs have been known to reside in salivary glands, the precise location and roles of AQPs have been not well examined. This study is aimed to investigate the distribution of AQPs in 3 major salivary glands and their changes after cholinergic stimulation using immunohistochemical study in Sprague Dawley rats weighing 300 g under pentobarbital sodium anesthesia. AQP1 was localized in the endothelial cells of all salivary capillary vessels and the myoepithelial cells. AQP4 was demonstrated in the epithelium of the excretory ductal cells of all salivary glands. AQP5 and 8 were abundantly present in the basolateral membrane and apical membranes of the serous acini including intercellular secretory canaliculi, whereas AQP5 was weakly present in mucous acini. In addition, AQP5 was found in the epithelium of the intercalated and striated ducts. Upon stimulation of carbachol (10 μg/kg, I.P). AQP5 and 8 tended to translocate from basolateral membrane to the apical membrane, appearing as clusters of dots. These results suggest that AQP5 and 8 are the candidate molecules responsible for the water movement in salivary acinar cells.

A Study on the Drag Reduction of Viscosity Flow by Heat Flux Change under Non-Boiling

Kim, Jae-Ho,Cha, Kyung-Ok 明知大學校 産業技術硏究所 2003 産業技術硏究所論文集 Vol.22 No.-

Abstract - When a water soluble polymer is applied to various kinds of the fluid transportation systems, it is predicted that the application could be used for the progress of the pump capacity and so forth in the fluid transportation. But in the condition of the poorly evolved theories concerning the development of the turbulence in the turbulent flow of the viscosity fluid and the dynamics of polymer, actually it is very difficult to develop the fixed quantity analysis. When a polymer solution is also flowing in the fluid transportation system, the degradation which has very close relation with the phenomena of the drag reduction occurs necessarily. This study has been focussing on a searching examination for the experimental study considering the mechanical and the thermal degradation in the closed system to find out the conditions which could improve the result, under non-boiling, we have found out that the change of the heat flux has a little effect on the drag reduction.

Water Extract of <i>Cordyceps militaris</i> Enhances Maturation of Murine Bone Marrow-Derived Dendritic Cells <i>in Vitro</i>

Kim, Gi-Young,Ko, Woo-Shin,Lee, Jae-Yoon,Lee, Jeong-Ok,Ryu, Chung-Ho,Choi, Byung Tae,Park, Yeong-Min,Jeong, Young-Ki,Lee, Kyeong-Jun,Choi, Kwang-Sik,Heo, Moon-Soo,Choi, Yung Hyun Pharmaceutical Society of Japan 2006 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.29 No.2

<P>Water extract (WE) of <I>Cordyceps militaris</I> has been reported to produce antitumor and immunomodulatory activities <I>in vivo</I> and <I>in vitro</I>. However, the therapeutic mechanism has not been known. In this study, we investigated whether water extract of <I>C. militaris</I> induces the phenotypic and functional maturation of dendritic cells (DC). It profoundly increased CD40, CD54, CD80, CD86, and MHC class II expression in murine bone marrow (BM)-derived myeloid DC. Endocytosis was assessed by the uptake of FITC-dextran and FITC-albumin. The ability of unstimulated DC (UT-DC) to uptake dextran and albumin was higher than that of WE- or LPS-stimulated DC (LPS-DC). Also, UT-DC secreted a low concentration of IL-12, while WE- or LPS-DC secreted higher levels of IL-12 than UT-DC. WE not only formed morphologically mature DC and clusters, but also induced predominantly functional maturation. Moreover, WE is shown to promote the cytotoxicity of specific-cytotoxic T lymphocyte (CTL) induced by DC which were pulsed with P815 tumor-lysate during the stage of antigen presentation. These results suggest that DC maturation by WE can play a critical role in the improvement of the immunoregulatory function in patients with impaired host defense.</P>

Analysis of the Fine-Scale Population Structure of “Candidatus Accumulibacter phosphatis” in Enhanced Biological Phosphorus Removal Sludge, Using Fluorescence In Situ Hybridization and Flow Cytometric Sorting

Kim, Jeong Myeong,Lee, Hyo Jung,Kim, Sun Young,Song, Jae Jun,Park, Woojun,Jeon, Che Ok American Society for Microbiology 2010 Applied and environmental microbiology Vol.76 No.12

<B>ABSTRACT</B><P>To investigate the fine-scale diversity of the polyphosphate-accumulating organisms (PAO) “<I>Candidatus</I> Accumulibacter phosphatis” (henceforth referred to as “<I>Ca.</I> Accumulibacter”), two laboratory-scale sequencing batch reactors (SBRs) for enhanced biological phosphorus removal (EBPR) were operated with sodium acetate as the sole carbon source. During SBR operations, activated sludge always contained morphologically different “<I>Ca</I>. Accumulibacter” strains showing typical EBPR performances, as confirmed by the combined technique of fluorescence <I>in situ</I> hybridization (FISH) and microautoradiography (MAR). Fragments of “<I>Ca.</I> Accumulibacter” 16S rRNA genes were retrieved from the sludge. Phylogenetic analyses together with sequences from the GenBank database showed that “<I>Ca.</I> Accumulibacter” 16S rRNA genes of the EBPR sludge were clearly differentiated into four “<I>Ca.</I> Accumulibacter” clades, Acc-SG1, Acc-SG2, Acc-SG3, and Acc-SG4. The specific FISH probes Acc444, Acc184, Acc72, and Acc119 targeting these clades and some helpers and competitors were designed by using the ARB program. Microbial characterization by FISH analysis using specific FISH probes also clearly indicated the presence of different “<I>Ca.</I> Accumulibacter” cell morphotypes. Especially, members of Acc-SG3, targeted by probe Acc72, were coccobacillus-shaped cells with a size of approximately 2 to 3 μm, while members of Acc-SG1, Acc-SG2, and Acc-SG4, targeted by Acc444, Acc184, and Acc119, respectively, were coccus-shaped cells approximately 1 μm in size. Subsequently, cells targeted by each FISH probe were sorted by use of a flow cytometer, and their polyphosphate kinase 1 (<I>ppk1</I>) gene homologs were amplified by using a <I>ppk1</I>-specific PCR primer set for “<I>Ca.</I> Accumulibacter.” The phylogenetic tree based on sequences of the <I>ppk1</I> gene homologs was basically congruent with that of the 16S rRNA genes, but members of Acc-SG3 with a distinct morphology comprised two different <I>ppk1</I> genes. These results suggest that “<I>Ca.</I> Accumulibacter” strains may be diverse physiologically and ecologically and represent distinct populations with genetically determined adaptations in EBPR systems.</P>

Phylogenetic Relationship of Cheju Native Horses by Mitochondrial DNA Analysis

Kim, Gi Ok,Ko, Mi Hee,Hong, Sung Soo,Oh, You Sung,Oh, Moon You,Kim, Se Jae 濟州大學校 基礎科學硏究所 1994 基礎科學硏究 Vol.7 No.1

The RELP patterns of Cheju native horse's mtDNA were analyzed in 52 head from the Cheju (Korea) population using 18 different restriction enzymes. Of the 18 different enzymes used, polymorphism of the cleavage patterns was observed in 4 enzyme (BamHI, EcoRI, HindIII, and AvaII) digestions. By combining the cleavage patterns for each enzyme, 52 head were classified into 9 restriction types. The average number of nucleotide substitution per site (δ) was estimated to be 0.0104. The phylogenetic tree constructed from the 9 restriction types by UPGMA method indicated that mtDNAs of the 52 Cheju native horses are divided into two clusters which diverged approximately 7.2×10 5 years ago. The comparison of mtDNA cleavage maps of Cheju native horses with those of other species showed that each species contained an average of 55 cleavage sites for 15 enzymes, of which 25 were invariant for all species. Trees constructed according to the parsimony principle, on the basis of 34 phylogenetically informative restriction sites, indicated that Cheju native horse was more closely related to E. przewalskii among species of Equus. We suggest that RFLP analysis of mtDNA is a useful tool for elucidating the origin of Cheju native horses and phylogenetic relationships among species of Equus.

Risk Factor Associated with Acquired hypofibrinogenemia in Patients with Hemocoagulase Administration for Hemoptysis

( Jae-kyeong Lee ),( Hong-joon Shin ),( Young-ok Na ),( Hwa-kyung Park ),( Hyung-joo Oh ),( Bo-gun Kho ),( Tae-ok Kim ),( Yong-soo Kwon ),( Yu-il Kim ),( Sung-chul Lim ),( Min-seok Kim ),( Cheol-kyu P 대한결핵 및 호흡기학회 2021 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.129 No.-

Purpose Hemocoagulase, a hemostatic, is used in patients with trauma, gastrointestinal bleeding, or pulmonary hemorrhage or those undergoing surgery. However, cases of acquired hypofibrinogenemia and paradoxical bleeding associated with hemocoagulase administration have been reported. In this study we evaluate the risk factors associated with acquired hypofibrinogenemia in patients with hemocoagulase batroxobin administration for hemoptysis. Methods We reviewed medical charts of hospitalized patients receiving hemocoagulase batroxobin for hemoptysis from January 2020 to April 2021 at a tertiary hospital. Acquired hypofibrinogenemia was defined as a plasma fibrinogen level more than 200 mg/ dL at baseline and decreased to less than 200 mg/dL after hemocoagulase administration. Results Total 186 patients were enrolled. Mean age was 68.4 ± 13.9 years, and male was predominant (63.4%). Most common cause of hemoptysis was bronchiectasis (59%). Mean duration of batroxobin administration was 3.5 ± 0.2 days and mean dosage of batroxobin was 35.3 ± 2.0 U. One hundred six patients (57.0%) developed acquired hypofibrinogenemia after administration of batroxobin. Recurrence of bleeding during hospitalization was more frequent in patients with acquired hypofibrinogenemia (22.6% vs. 6.3%; P=0.002). Multivariate analysis with logistic regression revealed longer duration of batroxobin administration was associated with acquired hypofibrinogenemia (odds ratio, 1.40; 95% confidence interval, 1.12-1.74; P=0.003). Conclusion Long duration of hemocoagulase use is associated with acquired hypofibrinogenemia and increased risk of rebleeding. Therefore, it is necessary to measure plasma fibrinogen levels regularly in patients with hemocoagulase administration.

[P10-221] Comparisions on Antioxidant Activities of Various Kind of Seaweeds

Jae Hak Shon,Dae-Yeon Kang,Jung Ae Park,Mi-Ok Shin,Mihyang Kim,Song-Ja Bae 한국식품영양과학회 2005 한국식품영양과학회 학술대회발표집 Vol.2005 No.10

Electroluminescent Characteristics of Conjugated Polymer/Ionomer Blend Devices According to Ionic Contents

Kim, Tae-Ho,Park, O Ok,Yu, Jae-Woong,Kim, Jai Kyeong TaylorFrancis 2006 MOLECULAR CRYSTALS AND LIQUID CRYSTALS - Vol.444 No.1

<P>Fluorescent polymer and poly (sodium 4-styrenesulfonate) ionomer (PSS-ionomer) blends were used as emitting layers in polymer light-emitting diodes (LEDs). The two polymers were blended in the optimal ratio which shows the highest luminescent intensity due to dilution effect. We changed the ionic contents of ionomer from 5 mol% to 10 mol% with fixed optimal blended ratio. We obtained the narrow spectrum having high color purity in the blend system. The conjugated polymer/PSS-ionomer blend devices achieved a more enhanced luminescent efficiency as compared with that of the conjugated polymer/PS device due to polar groups in the PSS-ionomer, which enhance the electron injection from the cathode to the emitting molecules.</P>