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Pseudomonas sabulinigri sp. nov., isolated from black beach sand
Kim, K.-H.,Roh, S. W.,Chang, H.-W.,Nam, Y.-D.,Yoon, J.-H.,Jeon, C. O.,Oh, H.-M.,Bae, J.-W. Microbiology Society 2009 International journal of systematic and evolutiona Vol.59 No.1
<P>A novel Gram-negative, aerobic, motile, short rod-shaped bacterium, designated J64T, was isolated from black sand collected from Soesoggak, Jeju Island, Korea. Cells grew at 4-37 degrees C, at pH 5.5-10.0 and with 0-10 % NaCl. The strain was found to be oxidase- and catalase-positive. Phylogenetic analyses showed that strain J64T belongs to the genus Pseudomonas, forming a monophyletic group with Pseudomonas pachastrellae, Pseudomonas pertucinogena and 'Pseudomonas denitrificans'. The 16S rRNA gene sequence similarity between strain J64T and type strains of all Pseudomonas species with validly published names was below 96.6 %. Low levels of DNA-DNA relatedness were found with respect to type strains of P. pachastrellae and P. pertucinogena, supporting the classification of strain J64T within a novel species of the genus Pseudomonas. Strain J64T contained C(18 : 1)omega7c (37.2 %), C(16 : 0) (20.4 %), summed feature 3 (17.4 %; comprising iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c) and C(12 : 0) (7.6 %) as major cellular fatty acids. On the basis of the phenotypic and phylogenetic data, strain J64T represents a novel species of the genus Pseudomonas, for which the name Pseudomonas sabulinigri sp. nov. is proposed. The type strain is J64T (=KCTC 22137T =JCM 14963T).</P>
Sphingomonas aestuarii sp. nov., isolated from tidal flat sediment
Roh, S. W.,Kim, K.-H.,Nam, Y.-D.,Chang, H.-W.,Kim, M.-S.,Oh, H.-M.,Bae, J.-W. Microbiology Society 2009 International journal of systematic and evolutiona Vol.59 No.6
<P>A novel bacterium (strain K4(T)) belonging to the genus Sphingomonas was isolated from tidal flat sediment in Korea. Its morphology, physiology, biochemical features and 16S rRNA gene sequence were characterized. Colonies of this strain are yellow in colour and the cells are rod-shaped, exhibiting negative Gram staining. The strain grows at 0-5 % (w/v) NaCl and 20-35 degrees C, with optimal growth occurring at 0 % (w/v) NaCl and 30 degrees C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain K4(T) is associated with the genus Sphingomonas. Within the phylogenetic tree, this novel strain shares a branching point with Sphingomonas asaccharolytica Y-345(T), with which it shares 97.3 % 16S rRNA gene sequence similarity. The polyamine pattern predominantly contains the Sphingomonas-specific triamine sym-homospermidine. Combined analysis of 16S rRNA gene sequences, DNA-DNA relatedness, physiological and biochemical test results identified genotypic and phenotypic differences between strain K4(T) and other Sphingomonas species. On the basis of these differentiating features, it is concluded that strain K4(T) (=KCTC 22050(T)=DSM 19475(T)) represents a novel species of the genus Sphingomonas, for which the name Sphingomonas aestuarii sp. nov. is proposed.</P>
Gill, S.S.,Oh, H.W.,Lee, D.W.,Roh, J.Y.,Park, H.W.,Jin, B.R.,Je, Y.H.,Kang, S.K. 東亞大學校附設遺傳工學硏究所 1998 遺傳工學硏究 Vol.- No.5
J.Y.ROH,H.W.PARK,Y.H.JE,D.W.LEE,B.R.JIN, H.W.OH,S.S.GILL AND S.K.KANG.1997. Bacillus thuringiensis NTB-1 isolated from soil samples in Korea produces ovoidal parasporal inclusions with proteins of approximately 24-40kDa in size. Although serological study indicated that the isolate has a flagella (H) antigen identical with subsp. israelensis, it seemed to be non-insecticidal against Lepidoptera and Coleoptera as well as Diptera. To investigate the activity of non-insecticidal B.thuringiensis transformed with insecticidal crystal protein genes, cryIVD and cytA genes of B. thuringiensis subsp. morrisoni PG-14, highly toxic to mosquito larvae, were introduced into the isolate NTB-1. The expression of mosquitocidal crystal protein genes in NTB-1 was characterized by SDS-PAGE analysis and electron microscopy. The results showed that crystalline inclusions of host, CryIVD and CytA were stably expressed in the transformant. However, the mosquitocidal activity of transformant was similar to that of B. thuringiensis subsp. kurstaki Cry-B harbouring cryIVD and cytA genes, demonstrating that a synergistic effect by an interaction of both introduced insecticidal and resident non-insecticidal crystal proteins was not observed.
Roh, H.J.,Kim, S.Y.,Min, B.K.,Kim, J.N. Pergamon Press ; Elsevier Science Ltd 2017 Tetrahedron letters: the international organ for t Vol.58 No.1
Various dihydrofuranyl spirooxindoles have been synthesized via montmorillonite K-10-catalyzed propargylation of 1,3-dicarbonyl compounds with isatin-derived propargylic alcohols and subsequent base-mediated 5-exo-dig cyclization.
HBsAg에 오염된 혈액검체에 대한 化學的 消毒劑의 效果에 關한 硏究
함건주,이정녀,조종래,노상옥,김공현 인제대학교 1993 仁濟醫學 Vol.14 No.1
병원에서 각종 진단 목적에 이용되는 환자의 혈액 검체 내에는 세균, 바이러스 등의 병원성 미생물에 감염된 것이 많다. 따라서 본 실험은 검사를 마친 이들 검체의 대부분이 적절한 살균 조작을 거치지 않음으로써 유발될 수 있는 환경오염이나 질병의 전파 등 보건위생상 중대한 문제를 예방하고자 B형 간염 항원 양성 혈장을 사용하여 여러가지 소독제의 바이러스에 대한 불활성화 능력을 방사 면역 측정법(Radioimmunoassay, RIA)으로 측정하였다. 실험 결과는 반응 시간 7시간인 경우 2% 농도에서 benzalkonium chloride가 98.6%로 가장 높았고, glutarldehyde는 92.3%, MLDGH는 88.6%였으며, NaOCl은 68.8%의 순서로 나타났다. 따라서 2% benzalkonium chloride를 사용하는 것이 가장 효과적인 것으로 평가되었다. Contaminated blood with microorganism and virus is put into the environment without any discontamination treatment. It may give rise to a serious health and sanitation problems and also to a threat of spreading infectious disease. In order to select the appropriate disinfectant and disinfection procedure, the teats performed in vitro via artificially contaminated blood with hepatitis B surface antigen(HBs Ag) positive plasma using several disinfectants commonly used in hospitals. The tested disinfectants were sodium hypochlorite(NaOCL), sodium dichloroisocyanurate(NaDCC), ethyl alcohol(EtOH), povidon iodine, cresol soap, benzalkonium chloride, mono-laurel-di(aminoethyl)-glycin HCI(MLDGH) and glutaraldehyde. The HBs Ag inactivation study was performed using the Radioimmunoassay(RIA) method. The results were as follows ; The inactivation effect on the HBs Ag of the benzalkonium chloride, glutaraldehyde, MLDGH, and NaOCL was 98.6%. 92.3%, 88.6% and 68.8% with in 5 hours after disinfection on the same 2% concentration, respectively. In conclusion, this study provided useful information for the 2% benalkonium chloride is the best disinfectants in consideration of the chemical stability, economy, and sanitation.
Nocardioides basaltis sp. nov., isolated from black beach sand
Kim, K.-H.,Roh, S. W.,Chang, H.-W.,Nam, Y.-D.,Yoon, J.-H.,Jeon, C. O.,Oh, H.-M.,Bae, J.-W. Microbiology Society 2009 International journal of systematic and evolutiona Vol.59 No.1
<P>A novel Gram-positive, aerobic, short-rod-shaped bacterium, designated strain J112T, was isolated from black sand collected from Soesoggak, Jeju Island, Korea. The strain was found to be oxidase-negative and catalase-positive. Cells grew at 10-37 degrees C, at pH 5.5-8.0 and with 1-10 % NaCl. Growth occurred on marine agar but not on R2A or trypticase soy agar. A phylogenetic analysis based on 16S rRNA gene sequences showed that the strain belongs to the radiation of the genus Nocardioides. Strain J112T shared the highest 16S rRNA gene sequence similarities with Nocardioides marinisabuli SBS-12T (99.2 %), Nocardioides terrigena DS-17T (97.3 %), Nocardioides kribbensis KCTC 19038T (97.1 %) and type strains of other Nocardioides species with validly published names (<97 %). The DNA-DNA hybridization values between strain J112T and the three most closely related strains were low enough to justify the assignment of this strain to a novel species. On the basis of these phenotypic, phylogenetic and chemotaxonomic data, strain J112T represents a novel species of the genus Nocardioides, for which the name Nocardioides basaltis sp. nov. is proposed. The type strain is J112T (=KCTC 19365T=JCM 14945T).</P>
초정밀 평면 X-Y 스테이지의 시뮬레이션 및 제어성능 평가
곽이구,김재열,한재호,김영석,안재신,노기웅 한국공작기계학회 2002 한국공작기계학회 춘계학술대회논문집 Vol.2002 No.-
In this study, actuator, sensor, guide, power transmission element and control method are considered for ultra-precision positioning apparatus. Through previous process, single plane X-Y stage with ultra-precision positioning is manufactured. Global stage for the purpose of materialization with robust system, is combined by using AC servo motor and ball screw and rolling guide. And ultra-precision positioning system is developed by micro stage with elastic hinge type and piezo element. global servo and micro servo for the purpose of materialization positioning accuracy with nm(nanometer) are controlled simultaneously by using incremental encoder and laser interferometer as displacement measurement sensor. Through previous process, ultra-precision positioning system (100mm stroke and ±10nm positioning accuracy) with single plane X-Y stage are materialized.
중년 사무직 남성의 직무스트레스와 허리둘레, 체질량지수의 관계에 관한 연구
강수임,고보경,김지영,노정연,리청,송주은,이현영,이혜민,최유경,정덕유,이민경 이화여자대학교 간호과학대학 2016 이화간호학회지 Vol.- No.50
Purpose: The purpose of this study was to determine the relationship between job stress, body mass index and waist circumference among middle aged male workers. Methods: Using a descriptive correlation study design, a total of 122 middle aged male workers were recruited through convenience sampling from November 8 to December 13, 2015. The questionnaire included in this study were general characteristics and the Korean Occupational Stress Scale-Short Form (KOSS-SF). Data were statistically analyzed using SPSS windows version 22 program with Pearson’s correlation coefficient. Results: A significant relationship was found between job stress, body mass index (p=.043) and waist circumference (p=.007). Conclusion: These results suggest that high job stress are significantly associated with both body mass index and waist circumference of middle aged male workers. This implies a need for intervention that focuses on reducing obesity and metabolic syndrome influenced by job stress.