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Md. Fokhrul Islam, Wonjae Lee 조선대학교 기초과학연구원 2015 조선자연과학논문집 Vol.8 No.2
Liquid chromatographic enantiomer separation of α-amino acid esters as nitrobenzoxadiazole (NBD) derivatives was performed using several chiral stationary phases (CSPs) based on polysaccharide derivatives under fluorescence detection. For enantiomer separation by normal HPLC, the non-aqueous derivatization method of α-amino acid esters for NBD analytes was introduced. Among the six CSPs used in this study, the performance of Chiralpak IA was superior for enantiomer resolution of NBD derivatives of several α-amino acid methyl esters. Also the convenient analytical method using polysaccharide-derived CSPs developed in this study was applied to determine the optical purity of α-amino acids esters. It was investigated that the enantiomeric impurity levels of 0.02-1.73% were found after determination of enantiomeric purities of several commercially available L-amino acid methyl esters. It is expected to be quite useful for enantiomer separation of other α-amino acid esters as NBD derivatives by normal HPLC.
Islam, Md. Fokhrul,Lee, Wonjae The Basic Science Institute Chosun University 2015 조선자연과학논문집 Vol.8 No.2
Liquid chromatographic enantiomer separation of ${\alpha}$-amino acid esters as nitrobenzoxadiazole (NBD) derivatives was performed using several chiral stationary phases (CSPs) based on polysaccharide derivatives under fluorescence detection. For enantiomer separation by normal HPLC, the non-aqueous derivatization method of ${\alpha}$-amino acid esters for NBD analytes was introduced. Among the six CSPs used in this study, the performance of Chiralpak IA was superior for enantiomer resolution of NBD derivatives of several ${\alpha}$-amino acid methyl esters. Also the convenient analytical method using polysaccharide-derived CSPs developed in this study was applied to determine the optical purity of ${\alpha}$-amino acids esters. It was investigated that the enantiomeric impurity levels of 0.02-1.73% were found after determination of enantiomeric purities of several commercially available L-amino acid methyl esters. It is expected to be quite useful for enantiomer separation of other ${\alpha}$-amino acid esters as NBD derivatives by normal HPLC.
Md. Fokhrul Islam,Suraj Adhikari,백만정,이원재 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.3
The liquid chromatographic enantiomer separationof various chiral acids as 1-naphthylamides was performedusing several chiral stationary phases (CSPs). TheCSPs used in this study were six covalently bonded andfour coated type CSPs derived from amylose and cellulosederivatives as chiral selectors. The degree of enantioseparationis affected by the structure of chiral acids and theCSPs used, which have different chiral selectors and typesof immobilization. For the enantiomer resolution of chiralacids as 1-naphthylamide derivatives, the performance ofthe coated type Lux Cellulose-1 was superior to those ofthe other CSPs, except for 2-aryloxypropionic acidderivatives. Owing to the strong ultraviolet absorbance ofthe 1-naphthyl group, the convenient analytical methoddeveloped and validated in this study could be expected tobe very useful for the enantiomer separation of variouschiral acids as 1-naphthylamide derivatives usingpolysaccharide-derived CSPs.
Md. Fokhrul Islam,Suraj Adhikari,백만정,이원재 대한화학회 2019 Bulletin of the Korean Chemical Society Vol.40 No.4
Liquid chromatographic enantiomer separation and simultaneous determination of chemical and enantiomeric purity of α-amino acids and their methyl esters as N-fluorenylmethoxycarbonyl (FMOC) derivatives was performed on three covalently bonded type chiral stationary phases (CSPs) derived from amylose derivatives. The enantiomer separation of α-amino acid esters as N-FMOC derivatives was better than that of the corresponding acids, especially for CSP 1 and 2. Chemical impurities as the corresponding racemic acids present in several commercially available racemic amino acid methyl esters were observed to be 0.49?17.50%. Enantiomeric impurities of several commercially available L-amino acid methyl esters were found to be 0.03?0.58%, whereas chemical impurities as the corresponding racemic acids present in the same analytes were found to be 0.13?13.62%. This developed analytical method will be useful for the determination of chemical and enantiomeric purity of α-amino acids and/or esters as N-FMOC derivatives using amylose-derived CSPs.
고성능 액체 크로마토그래피에서 아미노산과 이들 에스테르의 플루오레닐메톡시카르보닐 유도체의 자외선과 형광 검출에서의 동시 광학분리
이스람 포크롤(Md. Fokhrul Islam),이원재(Wonjae Lee) 한국생물공학회 2015 KSBB Journal Vol.30 No.4
Liquid chromatographic enantiomer separation of α-amino acids and their methyl and ethyl esters as fluorenylmethoxycarbonyl (FMOC) derivatives was performed using a recently developed chiral column (Chiralpak IE) based on polysaccharide derivative under simultaneous UV detection and fluorescence detection. The degree of enantiomer separation of α-amino acid esters as FMOC derivatives is generally higher than that of the corresponding α-amino acids. Especially, α-amino acid methyl esters showed the greatest enantioseparation. As this method developed in this study can be applied to determine the chemical and optical purity of α-amino acids and esters, it is expected to be quite useful for their chiral separation using Chiralpak IE.
Seok Jin Kim(김석진),Kyung Wook Nam(남경욱),Bohyun Park(박보현),Md. Fokhrul Islam(이스람 포크롤),Wonjae Lee(이원재) 한국생물공학회 2016 KSBB Journal Vol.31 No.3
A liquid chromatographic method for the enantiomer separation of fluoxetine was performed using covalently bonded and coated type polysaccharide-derived chiral stationary phases (CSPs). The degree of enantioseparation is affected by the used CSPs and mobile phases. The performance of Chiralpak IC was superior to the other CSPs used in this study. Out of various solvent composition and additives, the greatest separation and resolution was observed using Chiralpak IC with mobile phase of 2-propanol in hexane with diethylamine as an additive. Semi-preparative separation of fluoxetine was performed on the analytical Chiralpak IC column to obtain (R)- and (S)-fluoxetine enantiomer with high chemical and optical purity. From the overall study, the developed liquid chromatographic method on polysaccharide-derived CSPs is expected to be very useful for the enantiomer separation of fluoxetine.
Mou Taslin Jahan,Nupur Nasrin Akter,Haque Farhana,Islam Md Fokhrul,Rahman Md. Shahedur,Huq Md. Amdadul,Parvez Anowar Khasru 한국미생물·생명공학회 2023 한국미생물·생명공학회지 Vol.51 No.3
The pharmaceutical industry in Bangladesh produces a diverse range of antibiotics for human and animal use, however, waste disposal management is inadequate. This results in substantial quantities of antibiotics being discharged into water bodies, which provide suitable environment for the growth of antibiotic-resistant bacteria, capable of spreading resistance genes. This study intended for exploring the bacterial antibiotic resistance profile in adjoining aquatic environmental sources of pharmaceutical manufacturing facilities in Bangladesh. Seven surface water samples were collected from the vicinity of two pharmaceutical industries located in the Savar area and 51 Escherichia coli isolates were identified using both phenotypic and genotypic methods. Antibiotic susceptibility tests revealed the highest percentage of resistance against ampicillin, azithromycin, and nalidixic acid (100%) and the lowest resistance against meropenem (1.96%) out of sixteen different antibiotics tested. 100% of the study E. coli isolates were observed with Multidrug resistance phenotypes, with the Multiple Antibiotic Resistance (MAR) value ranging from 0.6-1.0. Furthermore, 69% of the isolates were Extended Spectrum Beta-Lactamases (ESBL) positive as per the Double Disk Diffusion Synergy Test (DDST). ESBL resistance genes blaTEM, blaCTX-M-13, blaCTX-M-15, and blaSHV were detected in 70.6% (n = 36), 60.8% (n = 32), 54.9% (n = 28), and 1.96% (n = 1) of the isolates, respectively, by Polymerase Chain Reaction (PCR). Additionally, 15.68% (n = 8) of the isolates were positive for E. coli specific virulence genes in PCR. These findings suggest that pharmaceutical wastewater, if not properly treated, could be a formidable source of antibiotic resistance spread in the surrounding aquatic environment. Therefore, continued surveillance for drug resistance among bacterial populations around drug manufacturing facilities in Bangladesh is necessary, along with proper waste disposal management.
김석진,남경욱,박보현,이스람 포크롤,이원재,Kim, Seok Jin,Nam, Kyung Wook,Park, Bohyun,Islam, Md. Fokhrul,Lee, Wonjae The Korean Society for Biotechnology and Bioengine 2016 KSBB Journal Vol.17 No.3
A liquid chromatographic method for the enantiomer separation of fluoxetine was performed using covalently bonded and coated type polysaccharide-derived chiral stationary phases (CSPs). The degree of enantioseparation is affected by the used CSPs and mobile phases. The performance of Chiralpak IC was superior to the other CSPs used in this study. Out of various solvent composition and additives, the greatest separation and resolution was observed using Chiralpak IC with mobile phase of 2-propanol in hexane with diethylamine as an additive. Semi-preparative separation of fluoxetine was performed on the analytical Chiralpak IC column to obtain (R)- and (S)-fluoxetine enantiomer with high chemical and optical purity. From the overall study, the developed liquid chromatographic method on polysaccharide-derived CSPs is expected to be very useful for the enantiomer separation of fluoxetine.