부산지역 수돗물과 지하수의 중금속 농도

김준연,손지언,김형수,김두희,원미숙,김인식,이혜령 동아대학교 산업의학연구소 2000 산업의학연구소 논총 Vol.- No.5

This study investigated mean airborne CO concention of 15 workplaces, suspected of CO exposure and conducted self-reported questionnaire completion and indirect COHb concentration measure using Micro II Smokerlyzer to healthy 702 adult subjects from 1999 May to 1999 September in order to find a relation of CO exposure and occupational factors, socioeconomic factors, and health related behaviors and confirm the related conditions in the screening test for CO exposure. The results of this study were summarized as follows : 1. In the CO exposed and non exposed group, COHb concentrations of the smokers were 2.55±0.96% and 2.21±0.97% and that got a statistically significant difference, There were not statistically significant differences in the age and total smoking index. Passed times after the last smoking, Working time/day, and working duration were statistically significant difference (p〈0.05). 2. In the CO exposed and non exposed group, COHb concentrations of non-smoking group were 0.94±0.35% and 0.68±0.47% and that got a statistically significant difference. There were not statistically significant differences in the age and working duration but Working time/day was a statistically significant difference(p〈0.05). 3. In the CO exposed group, r-square of multiple regression of the smokers was 38.5 % and passed time after the last smoking, working time/day, and job category were statistically significant differences (p〈0.05). And r-square in the CO non-exposed group was 38.3 % and age, passed time after the last smoking, and total smoking index were statistically significant difference. 4. In the CO exposed group, r-square of multiple repression of the non-smokers was 66.3% and job category and airborne CO concentration were statistically significant differences(p〈0.05). But r-square of non-smokers in the CO non-exposed group was 1.0% and there was not a statistically significant difference(p〈0.05). 5. In the smokers of CO exposed and non exposed groups, relation of COHb concentration and passed time after the last smoking was expressed as exponential function, Y = 2.9182e-0.0083x and r-square of this function was 37.4%. Therefore it was more than 150 minutes that passed time after the last smoking was when COHb concentrations were measured as less than 1%. In conclusion, variable, that was statistically significant to COHb concentraion in the both CO exposed and non-exposed smokers, was a passed time after the last smoking. We suggest that you have to restrict the smoking of smokers at least 150 minutes in the exposed and non exposed group before COHb concentration measure in order to exclude smoking effects

鼻알레르기의 호산구내 효소에 관한 전자현미경적 연구

吳世明,鄭泰仁,金基鈴 대한이비인후과학회 1977 대한이비인후과학회지 두경부외과학 Vol.20 No.3

Apoptotic Effect of Co-treatment with Chios Gum Mastic and HS-1200 on G361 Human Melanoma Cell Line

Young-Joo Hur,Young-Ki Kim,Hyun-Ho Kwak,Gyoo-Cheon Kim,Seung-Eun Lee,In-Ryoung Kim,Chul-Hoon Kim1,Bong-Soo Park 대한해부학회 2009 Anatomy & Cell Biology Vol.42 No.2

Chios gum mastic (CGM) is a resinous exudate obtained from the stem and the main leaves of Pistacia lenticulus tree native to Mediterranean areas. Recently it reported that CGM induce apoptosis in a few cancer cells in vitro. Bile acids and their synthetic derivatives induced apoptosis in various kinds of cancer cells and anticancer effects. It has been reported that the synthetic chenodeoxycholic acid (CDCA) derivatives showed apoptosis-inducing activity on various cancer cells in vitro. This study was undertaken to investigate the synergistic apoptotic effect of cotreatment with a natural product, CGM and a CDCA derivative, HS-1200 on G361 human melanoma cells. To investigate whether the co-treatment of CGM and HS-1200 compared with each single treatment efficiently reduced the viability of G361 cells, MTT assay was conducted. To investigate augmentation of apoptosis in G631 cells co-treated with CGM and HS-1200, DNA electrophoresis, Hoechst staining, proteasome activity assay, flow cytometry, Westen blot analyses, immunofluorescent staining and confocal microscopy were performed. In this study, G361 cells co-treated with CGM and HS-1200 showed several lines of apoptotic manifestation such as nuclear condensations, DNA fragmentation, the reduction of MMP and proteasome activity, the decrease of DNA content, the release of cytochrome c into cytosol, the translocation of AIF and DFF40 (CAD) onto nuclei, activation of caspase-9, caspase-3, PARP and DFF45 (ICAD), and up-regulation of Bax whereas each single treated G361 cells did not. Although the single treatment of 40 μg/mL CGM or 25 μM HS-1200 for 24 hrs did not induce apoptosis, the co-treatment of them induced prominently apoptosis. Therefore, combination therapy of CGM and HS-1200 could be considered, in the future, as an alternative therapeutic strategy for human melanoma.

상악동내에 발생한 미입견치의 1예

김기령,박인용,정진선 대한이비인후과학회 1976 대한이비인후과학회지 두경부외과학 Vol.19 No.3

비기질적인 흉통환자의 정신과적 임상 특성에 대한 연구

서세령,김진세,이현수,정인과 大韓神經精神醫學會 1996 신경정신의학 Vol.35 No.5

이 연구는 93년 9월에 5월까지 비기질적인 흉통 환자들중 정신과 진료를 거부한 22명, 정신과 진료를 받은25명과 대조 집단인 공황장애 환자 23명을 대상으로 하였다. 이들에게 불안 및 공포 상태를 평가하기 위해 Sheehan 임상 불안 척도, Spielberg 상태 - 특성 불안 검사, Sheehan 공포 척도와 우울정도를 평가하기위하여 Beck 우울 척도, 생활상의 장애를 평가하기 위해 사회 장애 척도 및 스트레스를 평가하기 위해 사회 재적응 평가 척도를 실시하였으며 다음과 같은 결과를 얻었다. 1) Sheehan 임상 불안 척도, Spielberg 특성 상태 불안 척도, Sheehan 공포 척도, Beck 우울 척도 및 사회 장애 척도에서 3군사이에 통계적으로 의미있는 차이는 없었다. 2) 사회 재적응 평가 척도에서 내과 환자가 공황장애 환자에 비해 통계적으로 유의하게 점수가 높았다. 이와 같은 결과로 비기질적인 흉통 환자들이 정신과적 장애가 있음을 강력히 시사하고 특히 최근 스트레스를 겪은 내과 환자에서 더욱 신속한 정신과적 평가와 치료가 중요하다는 것을 생각할 수 있다. 또한 이런 정신병리는 다시 기질적인 심장질환 발생의 원인이 되기도 하는 바 기기질적인 흉통 환자에 있어서 적극적인 정신과적 치료가 필요하다. Objective : We tried to compare and assess nonorganic chest pain patients and panic disorder patients in clinical manifestations. Methods : We compared the result of clinical scale for anxiety, depression, phobia and avoidance, stress, and social disability in twenty-two patients diagnosed to have nonorganic chest pain and having refused psychiatric consultation, twenty-five patients with nonorganic chest pain admitted to psychiatric department, and twenty-three panic disorder patients with no chest pain. Results : No significant difference was found among the three groups except social readjustment rating scale. Conclusion : The patients with nonorganic chest pain showed as much anxiety, depression, phobia and avoidance, stress, and social disability as the panic patients. Early psychiatric assessment and treatment of patients with nonorganic chest pain is warranted.

A Study of the pH Values of Discharges in Otolaryngology

Kim, Gill Ryoung,Kim, Sun Kon,Park, In Yong Yonsei University, College of Medicine 1962 Yonsei medical journal Vol.3 No.-

Influence of Propofol and Fentanyl on Deep Brain Stimulation of the Subthalamic Nucleus

Kim, Wonki,Song, In Ho,Lim, Yong Hoon,Kim, Mi-Ryoung,Kim, Young Eun,Hwang, Jae Ha,Kim, In Keyoung,Song, Sang Woo,Kim, Jin Wook,Lee, Woong-Woo,Kim, Han-Joon,Kim, Cheolyoung,Kim, Hee Chan,Kim, In Young The Korean Academy of Medical Sciences 2014 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.29 No.9

<P>We investigated the effect of propofol and fentanyl on microelectrode recording (MER) and its clinical applicability during subthalamic nucleus (STN) deep brain stimulation (DBS) surgery. We analyzed 8 patients with Parkinson's disease, underwent bilateral STN DBS with MER. Their left sides were done under awake and then their right sides were done with a continuous infusion of propofol and fentanyl under local anesthesia. The electrode position was evaluated by preoperative MRI and postoperative CT. The clinical outcomes were assessed at six months after surgery. We isolated single unit activities from the left and the right side MERs. There was no significant difference in the mean firing rate between the left side MERs (38.7±16.8 spikes/sec, n=78) and the right side MERs (35.5±17.2 spikes/sec, n=66). The bursting pattern of spikes was more frequently observed in the right STN than in the left STN. All the electrode positions were within the STNs on both sides and the off-time Unified Parkinson's Disease Rating Scale part III scores at six months after surgery decreased by 67% of the preoperative level. In this study, a continuous infusion of propofol and fentanyl did not significantly interfere with the MER signals from the STN. The results of this study suggest that propofol and fentanyl can be used for STN DBS in patients with advanced Parkinson's disease improving the overall experience of the patients.</P><P><B>Graphical Abstract</B></P><P/>

Anticancer Properties of Icariside II in Human Oral Squamous Cell Carcinoma Cells

In-Ryoung Kim,Young-Seok Kim,Su-Bin Yu,Hae-Mi Kang,Hyun-Ho Kwak,Bong-Soo Park 대한구강생물학회 2016 International Journal of Oral Biology Vol.41 No.1

OSCC is currently the most common malignancy of the head and neck, affecting tens of thousands of patients per year worldwide. Natural flavonoids from plants are potential sources for novel anti-cancer drugs. Icariin is the active ingredient of flavonol glycoside, which is derived from the medical plant Herba Epimedii. A metabolite of icariin, icariside II exhibits a variety of pharmacological actions, including anti-rheumatic, anti-depressant, cardiovascular protective, and immunomodulatory functions. However, the exact mechanism causing the apoptosis-inducing effect of icariside II in OSCC is still not fully understood. In the present study, we assessed the anti-cancer effect of icariside II in OSCC cell lines by measuring its effect on cell viability, cell proliferation, and mitochondria membrane potential (MMP). Icariside II treatment of OSCC cells resulted in a dose- and time-dependent decrease in cell viability. Hoechst staining indicated apoptosis in icariside II-treated HSC cells. Icariside II inhibited cell proliferation and induced apoptosis in HSC cells, with significant increases in all present parameters in HSC-4 cells. The results clearly suggested that icariside II induced apoptosis via activation of intrinsic pathways and caspase cascades in HSC-4 cell lines. The collective findings of the study suggested that Icariside II is a potential treatment for OSCC; in addition, the data could provide a basis for the development of a novel anti-cancer strategy.

The Electron Microscopic Study of Enzymes in Eosinophils

Kim, Gill Ryoung,Chung, Tae In,Choi, Seng Ee Yonsei University, College of Medicine 1975 Yonsei medical journal Vol.16 No.2

Changes in Cecal Microbiota and Short-chain Fatty Acid During Lifespan of the Rat

( Soo In Choi ),( Joo Hee Son ),( Nayoung Kim ),( Yong Sung Kim ),( Ryoung Hee Nam ),( Ji Hyun Park ),( Chin-hee Song ),( Jeong Eun Yu ),( Dong Ho Lee ),( Kichul Yoon ),( Huitae Min ),( Yeon-ran Kim ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2021 Journal of Neurogastroenterology and Motility (JNM Vol.27 No.1

Background/Aims The gut microbiota regulates intestinal immune homeostasis through host-microbiota interactions. Multiple factors affect the gut microbiota, including age, sex, diet, and use of drugs. In addition, information on gut microbiota differs depending on the samples. The aim of this study is to investigate whether changes in cecal microbiota depend on aging. Methods Gut microbiota in cecal contents of 6-, 31-, and 74-week-old and 2-year-old male Fischer-344 rats (corresponding to 5-, 30-, 60-, and 80-year-old humans in terms of age) were analyzed using 16S ribosomal RNA metagenome sequencing and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) based on the Kyoto Encyclopedia of Genes and Genomes orthology. Moreover, short-chain fatty acid (SCFA) level in cecum and inflammation related factors were measured using real-time quantitative polymerase chain reaction and enzyme linked immunosorbent assay. Results Alpha and beta diversity did not change significantly with age. At the family level, Lachnospiraceae and Ruminococcaceae, which produce SCFAs, showed significant change in 31-week-old rats: Lachnospiraceae significantly increased at 31 weeks of age, compared to other age groups, while Ruminococcaceae decreased. Butyrate levels in cecum were significantly increased in 31-week-old rats, and the expression of inflammation related genes was increased followed aging. Especially, EU622775_s and EU622773_s, which were highly abundance species in 31-week-old rats, showed significant relationship with butyrate concentration. Enzymes required for producing butyrate―acetyl-CoA transferase, butyryl-CoA dehydrogenase, and butyrate kinase―were not predicted by PICRUSt. Conclusions Major bacterial taxa in the cecal lumen, such as Lachnospiraceae, well-known SCFAs-producing family, changed in 31-week-old rats. Moreover, unknown species EU622775_s and EU622773_s showed strong association with cecal butyrate level at 31 weeks of age. (J Neurogastroenterol Motil 2021;27:134-146)