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盧一燮,金鎭漢 충북대학교 농업과학기술연구소 1990 農業科學硏究 Vol.8 No.1
This study was conducted to examine the effects of rhizosphere temperature on the growth of seedlings, internal and external morphology of leaves and absorption of mineral nutrients in pepper (Capsicum annuum L). Pepper seedlings were grown at rhizosphere temperature of 13℃, 18℃, 23℃, 28℃ and 33℃, respectively. Leaf thickness, chlorophyl content, number of chloroplasts, number of cells, size and number of palisade cells and number of stomata were measured and inoganic eleme-nts were analized. The results were summarized as follows ; 1.Seedling weight and fruit weight were heavier at 23℃, while they were reduced greatly at 13℃. 2.Leaf length and leaf width were largest at 23℃ and 28℃, respectively and both traits were became smaller as temperatures were increased or decreased. 3.Chlorophy Ⅱ content and number of chloroplasts were highest at 18℃ 4.Surface area and volume of palisade cells were significally greater at 23℃ and considerably reduced at 13℃. 5.Number of stomata was highest at 13℃,while no significant difference were noted among other temperatures. Number of stomata was about 4 times higher at abaxial side than at adaxial side. 6.Contents of inoganic nutrient analyzed except phosphate, such as T- N, K2O, MgO and CaO, in leaf, stem and root were higher at 23℃ and tended to be decreased at higher or lower temperatures.
참다래 수품종의 형태적 특성과 RAPD에 의한 유연관계 분석
조윤섭,조혜성,노일섭,김월수 전남대학교 농업과학기술연구소 2000 農業科學技術硏究 Vol.35 No.-
To examine the taxonomic relationships among 13 male kiwifruits by 36 morphological characters, principal component analysis and cluster analysis were carried out. Of 36 characters, hair density on ventral midribs and between ventral midribs, leaf length/leaf width ratio etc. were considered as very useful characters for the classification of Actinidiacea plants and number of white dots on branch, full flowering period and flower color etc. also showed significant differences among A. deliciosa, A. chinensis and wild kiwis. Results of cluster analysis using scores of the principal component indicated that 13 male kiwifruits could be grouped into A. deliciosa, A. chinensis and wild kiwis phenon at 0.5 of average distance in WMVCA. This result was also very similar to that of RAPD analysis. However, it seems that more characters including various kinds of Actinidia species are needed for the exact classification.
同形化型 自家不和合性에 관한 硏究動向 Ⅰ. 胞子體型 自家不和合性에 관한 遺傳分析 및 分子的 硏究
Ill Sup Nou(盧一燮),Hyo Yeon Lee(李孝淵),Jin Ho Kim(金晋鎬) 한국육종학회 1993 한국육종학회지 Vol.25 No.1
In self-incompatibility, the rejection if self pollen occurs at the initial site of pollination, the stigma, where pollen germination either does not commence or is aborted before invasion of the papillar cell wall by the pollen tube. Recently, analysis of self-incompatibility in Brassica has focused on the study of a class of stigma glycoproteins, the S-locus glycoprotein(SLG). The level of SLG corresponds with the onset of self-incompatibility and the polymorphisms of these molecules correlate with S-allele polymorphism and genetic segregation of S-allele. In these decades, knowledge have been accumulated explosively on S-alleles at DNA and protein levels. An evidence that the function of SLG was suggested by a database survey in respective systems tells us recent trends of studies in this area. These studies revealed the difference of the structure and function of S-alleles in sporophytic and gametophytic systems. Siganl transduction system as found in vertebrates seems to be involved in sporophytic incompatibility, since SRK(S-receptor kinase) genes were found. SRK gene was shown to encode a putative receptor protein kinase with a cytoplasmic domain exhibiting homology to serine/threonine protein kinase and an SLG-homologous extracelluar domain was speculated to be involved in ligand binding. Despite of the explosive increase of data, many important question are still unsolved What is the pollen specific substance that participate in the recognition reaction? Can variation of S-alleles be explained only by the structural difference of SLG? How is the whole structure of S-genes? These situation give us an impression that we are still very poverty in our knowledege of self-incompatibility, but the rush of papers in a couple of years gives us also a hope that the mechanism of self-incompatibility will be recongnized by us on a sound basis in very near future.
SEM observation the pollen-stigma interaction in self-incompatible strains of brassica campestris
Ill Sup Nou(盧一燮),Hyo Yeon Lee(李孝淵)Seon Ha Lee(李善河)Jin Ho Kim(金晉鎬)Kokichi Hinata(日向康吉) 한국육종학회 1994 한국육종학회지 Vol.26 No.2
The behavior of pollen and stigma was compared between self(incompatible)-and cross(compatible)-pollinations in connection with self-incompatibility in Brassica campestris L. through scanning electron microscope(SEM). Whithin 30 min after pollination, swollen pollen grains began to emerge a large quantity of secretion from one of the three furrows. The secretion covered the papilla cells and pollen altogether in the form of a film for about two hrs. The swelling was quicker and secretion was more abundant in the cross-pollination as compared with the self-pollinations. Recognition reaction between self and non-self occurs during the phase, but the discrimination doses not give conclusive effects. As the result of the recognition reaction, self-pollen tubes are unable to penetrate the papilla cell walls. Thus, the recognition reaction occurs at two phases ; pollen hydration-germination and pollen tube penetration. Although the self pollen tubes cannot penetrate papilla cells, the contact between a pollen papilla without breakage. This suggests that they were not separated from papilla without breakage. This suggests that a metabolic interaction has occurred between pollen tubes and papilla cells. On the other hand, the self pollen tubes elongated on the surface of papilla cells during night, indicating that they have a potential to elongate after the contact.