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RISS 인기검색어
- 검색키워드
- 저자 : Ill Sup Nou(盧一燮) (검색결과 2 건)
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SEM observation the pollen-stigma interaction in self-incompatible strains of brassica campestris
Ill Sup Nou(盧一燮),Hyo Yeon Lee(李孝淵)Seon Ha Lee(李善河)Jin Ho Kim(金晉鎬)Kokichi Hinata(日向康吉) 한국육종학회 1994 한국육종학회지 Vol.26 No.2
The behavior of pollen and stigma was compared between self(incompatible)-and cross(compatible)-pollinations in connection with self-incompatibility in Brassica campestris L. through scanning electron microscope(SEM). Whithin 30 min after pollination, swollen pollen grains began to emerge a large quantity of secretion from one of the three furrows. The secretion covered the papilla cells and pollen altogether in the form of a film for about two hrs. The swelling was quicker and secretion was more abundant in the cross-pollination as compared with the self-pollinations. Recognition reaction between self and non-self occurs during the phase, but the discrimination doses not give conclusive effects. As the result of the recognition reaction, self-pollen tubes are unable to penetrate the papilla cell walls. Thus, the recognition reaction occurs at two phases ; pollen hydration-germination and pollen tube penetration. Although the self pollen tubes cannot penetrate papilla cells, the contact between a pollen papilla without breakage. This suggests that they were not separated from papilla without breakage. This suggests that a metabolic interaction has occurred between pollen tubes and papilla cells. On the other hand, the self pollen tubes elongated on the surface of papilla cells during night, indicating that they have a potential to elongate after the contact.
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Detecting DNA markers by RFLP analysis and DNA-fingerprinting in capsicum
Dong Young Shin(申東永),Hak Soon Choi(崔學淳),Hyo Yeon Lee(李孝淵),Kwon Kyu Kang(姜權圭),Ill Sup Nou(盧一燮) 한국육종학회 1996 한국육종학회지 Vol.28 No.2
This experiment was carried out to detect of cultivar specific markers using RFLP analysis and DNA-fingerprinting in Capsicum genus. The result of SDS-PAGE analysis could not detect genotype specific protein markers workable within each species of Capsicum, it however was possible to detect some interspecies markers. For RFLP analysis and DNA-fingerprinting, DIG labelled (GATA)₄, 26S rRNA, rice chloroplast DNA clones (P1, P2, P10-1, P45, P50, B3), and maize mitochondrial DNA clone (ATP A) were used as a probe. With a (GATA)₄ probe the level of polymorphism detected was high enough to differentiate all 29 genotypes used in this study. The results with the most informative banding patterns were obtained with the (GATA)₄ probe hybridized to Hae Ⅲ, Rsa Ⅰ, and 26S rRNA probe hybridized to BamH Ⅰ, Hinf Ⅰ, Hae Ⅲ, Hind Ⅲ resticted genomic DNA, respectively. Hybridization with the P2 probe which produced polymorphic bands (11kb) in only Masanjere by HindⅢ digest. Mitochondrial ATP A probes revealed three variable band patterns among the 29 red pepper genotypes digested by Hind Ⅲ and HaeⅢ. A same band patterns was obtained between X-mas bell Ⅰ and X-mas bell Ⅱ on a HindⅢ digest. In case of Green 100 genotype specific band detcted by HindⅢ, HaeⅢ digested. Subsequently, the multilocus probe, (GATA)₄, 26S rRNA and ATP A probes has proven successful in distingulishing between different red pepper genotypes.
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