Overexpression of the 3’ half of the PHYB phytochrome partially suppresses dwarfism in the brassinosteroid-insensitive bri1-5 mutant

Yu Jeong Jeong,Soon Il Kwon,Slki Park,Su Jeoung Suh,Richard Cha,Yoong Eun Kim,Sunghwa Choe 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Brassinosteroids (BRs) control virtually every aspect of plant growth and development. BRs act alone or with other exogenous and endogenous signals including auxin and light. To screen for the novel player involved in BR signaling in Arabidopsis, we employed cDNA overexpression strategy. We created a cDNA library to be expressed under the 35S overexpression promoter, and introduced into a weak brassinosteroid insensitive 1 (bri1) mutant. The mutant dubbed bri1-5 with long petiole (blp) was identified to display bigger stature especially in hypocotyl and petiole length relative to bri1-5. Sequence analysis of the rescued transgene revealed that blp consisted of a chimeric DNA consisting of a 3’ half of PHYB, 2 bp insertion, and a part of a chloroplast ribosomal RNA. Re-introduction of chimeric DNA into bri1-5 recapitulated blp phenotype. The blp phenotypes being similar to phyB mutants led us to examine both the PHYB transcript and protein levels in the blp 35Spro:PHYB doubly homozygous line. Lower levels of both transcripts and proteins of PHYB suggested that introduction of the chimeric gene interfered with the stability of PHYB transcripts. Our results highlight that overexpression mutagenesis facilitates functional genomics to decipher a function of Arabidopsis genome.

The Effect of Cold Shock on Function and Morphology of Dog Epididymal Spermatozoa

Yu, Il-Jeoung,Songsasen, Nucharin,Kim, Yong-Jun,Kim, In-Shik,Kang, Hyung-Sub,Park, Jun-Ho 한국수정란이식학회 2006 한국수정란이식학회 학술대회 Vol.2006 No.1

The Effect of Cold Shock on Function and Morphology of Dog Epididymal Spermatozoa

Il-jeoung Yu 한국임상수의학회 2004 한국임상수의학회지 Vol.21 No.4

Dog spermatozoa were recovered from the caudae epididymides of 23 domestic dogs which were 11 purebreed and 12 mix-bred dogs ranging in age from 0.6 to 3 years. The experimental designs were as follows: 1) theeffect of chilling to 0, 10 or 37?C, 2) the kinetics of chilling Viable spermatozoa were recovered by percoll gradient separation and adjusted to 5. 107 spermatozoa/ml. In experiment1, spermatozoa were diluted with 0.33 M glucose suplemented with 3% BSA (G-BSA) at 1:2 dilution. Spermatozoawere loaded into straws and exposed at 0, 10 or 37C for 30 min. In experiment 2, spermatozoa were prepared as theexperiment 1 and exposed for 0.5, 5, 15, or 30 min at 0 or 4C. In experiment 3, spermatozoa were diluted with diferentsugars (0.33 M galactose, glucose, fructose, mannitol, lactose, sucrose, rafinose) and cooled to 0C for 30 min. Spermmembrane integrity, motility and acrosome integrity were assayed after rewarming at 37C for 5 min. Sperm motilityand membrane integrity abruptly decreased with decreasing temperature but acrosome integrity gradually decreased (P. cooled to 0?C were more damaged than those at 4?C. Sperm motility was not different among exposed times at both0 and 4C. However, membrane integrity of spermatozoa exposed for 30 min at both 0 and 4C was significantly lower(P. 0.05). Spermatozoa diluted in 0.33 M fructose or galactose showed lower motility and higher morphologicalabnormality with coiled tail at 0?C. These sperm characteristics were strongly related. These results indicate that dogepididymal spermatozoa are relatively sensitive to rapid cooling and higher morphological abnormality at 0C was shownin spermatozoa diluted in fructose and galactose. 냉각이 개 정자의 기능(생존력, 활력, 첨체고유성)과 형태(기형율)에 미치는 영향을 알아보기 위해 23마리 개의 정소상체에서 정자를 회수하여 다음과 같은 실험에 사용하였다. 정자를 3%BSA가 첨가된 0.3 M glucose (G-BSA)에 희석한 후 실험1에서 서로 다른온도, 0, 10, 37℃에 30분동안 냉각하여 37℃에서 가온한 후 정자의 활력, 생존력, 첨체고유성을 검사하였다.실험2에서 0℃와 4℃에서 정자를 서로 다른시간(5, 10, 15, 30분)동안 노출시켜 37℃에서 가온한 후 정자의 활력 및 생존력을 검사하였다. 또한 sugars가 개정자의 기능과 형태에 미치는 영향을 알아보기 위해 여러종류의 sugars (0.3 M)를 준비하고 0℃에서 정자를 sugar용액에 노출시켜 37℃에서 가온한 후 정자의 활력, 생존력 및 기형정도를 관찰하였다. 본 실험결과 개 정소상체유래의 정자는 냉각온도가 감소할수록 정자의 기능이 급격하게 감소하여 냉각에 민감하게 나타났다(P< 0.05).특히 정자의 활력이 다른 정자의 기능, 생존력과 첨체고유성에 비해 냉각에 민감하게 나타났다. 정자를 0℃와 4℃에서 서로 다른 시간(5, 10, 15, 30분)동안 냉각하였을 때 30분 동안 냉각된 정자가 그 외의 시간 동안 냉각된 정자에 비해 생존력이 저하되었다(P< 0.05). Sugars가 정자의 기능과 형태에 미치는 결과는 fructose와 galactose에 노출된 정자의 활력이 다른 sugars에 노출되었던 정자의 활력에 비해 현저히 낮았으며, 이와 관련되어 정자의 기형율이 높게 나타났다(P< 0.05). 이상의 결과 개 정소상체유래의 정자는 냉각에 비교적 민감하였으며 정자의 냉각에 대한 민감성을 완화시키는 첨가제로 단당류뿐만 아니라 다당류에 대한 연구가 이루어져야 할 것으로 생각된다.

Effect of 0.5 mM Dibutyryl cAMP on Meiotic Maturation during Different Incubation Time and Embryonic Development Following In Vitro Fertilization or Parthenogenetic Activation in Porcine Oocytes

Yu, Il-Jeoung 韓國受精卵移植學會 2011 한국동물생명공학회지 Vol.26 No.4

Presently, the effect of 0.5 mM dibutyryl cAMP (dbcAMP)-supplemented maturation medium during different incubation time on meiotic arrest (germinal vesicle) and resumption (metaphase II) of porcine oocytes and embryonic development of porcine oocytes following in vitro fertilization (IVF) or parthenogenetic activation (PA) was determined. Porcine cumulus oocyte complexes (COCs) were cultured in 0.5 mM dbcAMP for 17, 22, 27, or 42 h, and an additional 22 h without 0.5 mM dbcAMP. The nuclear status was examined at each time point. Oocytes cultured from 39~49 h displayed more than 80% meiotic resumption. More than 85 % of meiotic arrest was presented at 17~22 h. Oocytes were cultured for 22 h with 0.5 mM dbcAMP and additional 22 h without dbcAMP to assess developmental potential following IVF or PA. There were no significant differences in blastocyst rates among the dbcAMPIVF, IVF, dbcAMP-PA, and PA groups, although cleavage rate of IVF group was significantly higher than those of dbcAMP-PA, and PA groups. In conclusion, 0.5 mM dbcAMP influenced meiotic maturation of porcine oocytes depending on incubation time of oocyte, although embryonic development was not improved in both IVF and PA.

Evaluation of Toxicity of Green Tea Extract in Chilled Boar Spermatozoa

Sang-Hyoun Park,Il-Jeoung Yu 한국수정란이식학회 2015 한국동물생명공학회지 Vol.30 No.1

The cold shock of spermatozoa is associated with oxidative stress induced by reactive oxygen species. This study was conducted to evaluate the toxicity of natural antioxidant green tea extract (GTE) in lactose-egg yolk (LEY) extender during boar sperm cooling prior to freezing. Spermatozoa were cooled to 5℃ for 3 h in LEY extender containing 0 (control), 1, 10, 100 or 1,000 mg/l of GTE, re-suspended with LEY-glycerol-Equex extender and cooled at 5℃ for 30 min. Sperm progressive motility, viability and phosphatidylserine (PS) translocation were evaluated. PS translocation was assayed by flow cytometry using Annexin V-FITC apoptosis detection kit. The sperm function including progressive motility, viability and PS translocation was not significantly different regardless of GTE concentrations (P>0.05). In conclusion, this study demonstrated non-toxicity of GTE supplement in LEY extender during sperm cooling.

완전좌각차단(Complete LBBB)환자의 균형마취

임유택,권혁권,정병연,김세강,정용일 대한마취과학회 1996 Korean Journal of Anesthesiology Vol.31 No.6

Local or general anesthesia is essential for safe operation. Patients in good preoperative condition are relatively to tolerable to the operation, but the patients in poor preoperative condition have the high mortality and morbidity during and after the operation. Therefore, we should choose the anesthetic agents and methods, which cause little effect to the patients life. Among the intraventricular blocks, bundle branch is the most common type, and left bundle branch block may progress to a more serious condition of complete heart block. Optimal anesthetic management of patients with cardiovascular disease requires a thorough knowledge of normal cardiac physiology, the circulatory effects of the various anesthetic agents, and the pathophysiology and treatment of these diseases. The authors successfully performed balanced anesthesia in operation of a 63 year old female patient who showed complete left bundle branch block pattern in a preoperative electrocardiographic tracing without any subjective symptoms.

Evaluation of Toxicity of Green Tea Extract in Chilled Boar Spermatozoa

박상현,Il-Jeoung Yu 사단법인 한국동물생명공학회 2015 한국동물생명공학회지 Vol.30 No.1

The cold shock of spermatozoa is associated with oxidative stress induced by reactive oxygen species. This studywas conducted to evaluate the toxicity of natural antioxidant green tea extract (GTE) in lactose-egg yolk (LEY) extenderduring boar sperm cooling prior to freezing. Spermatozoa were cooled to 5℃ for 3 h in LEY extender containing0 (control), 1, 10, 100 or 1,000 mg/l of GTE, re-suspended with LEY-glycerol-Equex extender and cooled at 5℃ for30 min. Sperm progressive motility, viability and phosphatidylserine (PS) translocation were evaluated. PS translocation wasassayed by flow cytometry using Annexin V-FITC apoptosis detection kit. The sperm function including progressivemotility, viability and PS translocation was not significantly different regardless of GTE concentrations (P>0.05). Inconclusion, this study demonstrated non-toxicity of GTE supplement in LEY extender during sperm cooling.

노인환자에서 Taylor`s Approach를 통한 척추마취

정용일,정병연,임유택,하동춘 대한마취과학회 1997 Korean Journal of Anesthesiology Vol.33 No.5

The Taylor's approach is a special paramedian approach to enter the L5-S1 interspace. The L5-S1 interspace is the largest in vertebral column. This approach is particularly useful when the interspace has been narrowed by pathologic bone destruction such as rheumatoid arthritis or osteoarthritis. Surgery in geriatric patients is associated with a markedly higher incidence of perioperative complication or mortality rate. Optimal anesthetic management of geriatric patients depends on understanding of the normal anatomy and physiologic changes in response to drug in aging. We studied of 3-geriatric patients with Taylor's approach. These patients had problems with respiratory dysfunction and anatomic constraints, which make other approaches unfeasible. (Korean J Anesthesiol 1997; 33: 970∼973)

미추마취하의 수술시 Morphine과 혼합한 Mepivacaine, Bupivacaine의 투여에 따른 마취 발현시간 및 술후 제통효과의 비교

정용일,임유택,정병연,권혁권 대한마취과학회 1999 Korean Journal of Anesthesiology Vol.36 No.2

Background : Caudal injection of local anesthetics with morphine is the most common anesthetic technique for perianal operation and postoperative analgesia. This study was purposed to compare the onset time of caudal analgesia, postoperative analgesic effect and side effects. Method : Sixty healthy patients scheduled for perianal operation were divided into 2 groups randomly. Group I was given 2 mg of morphine in 20 ml of 2% mepivacaine via sacral hiatus. Group II was also given 2 mg of morphine in 20 ml of 0.5% bupivacaine caudally. We measured the onset time of caudal block, time to the first request of analgesics, the number of analgesics within 24 hours and the incidence of postoperative side effects. Analgesic effect was evaluated by visual analogue scales (VAS) at 1, 2, 6, 12 and 24 hours postoperatively. Result : The onset time of caudal block for operation and the first request time of analgesic for postoperative pain was significantly shorter in group I than group II. The analgesic use in the first 24 hours was significantly more in group I than group II. The side effects were similar in both groups. Conclusion : We concluded that the combined use of morphine and bupivacaine provided better postoperative analgesia than the combined use of morphine and mepivacaine. (Korean J Anesthesiol 1999; 36: 263∼267)

인공발정유도견의 발정기 변화와 생식기의 변화

유일정,김용준,Yu, Il-jeoung,Kim, Yong-jun 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.3

To investigate changes of estrus signs and genital organs in the bitch by hormonal induction of estrus, fourteen bitches of nulliparous and multiparous(2nd-5th) were grouped into diestrus and anestrus according to their estrus cycle. The hormonal treatments were divided into four groups: group A($PGF_2{\alpha}+PMSG+hCG$) and group B(PMSG+hCG) in diestrus bitches and group C(GnRH+FSH+hCG) and group D(PMSG+hCG) in anestrus bitches. The external signs of proestrus and estrus as well as the vaginal smear findings and natural breeding as estrus detection were investigated in all the experimental groups. Also, genital organs were examined at two months after the hormone treatment. The bitches in anestrus showed 100% of male attraction, vaginal bleeding and vulvar swelling as proestrus signs after the hormonal treatment for estrus induction and they showed higher numerical value of signs than the bitches in diestrus. The group A showed the lowest value in proestrous signs of all the groups. The bitches in anestrus treated with GnRH+FSH showed 100% of positive estrus by vaginal smear findings and 75% of natural breeding as estrus detection index and these values were the highest of all the groups. Pregnancy was recognized in only group C and the conception rate was 7.14% in al the experimental animals. Of the side effects after the hormone treatment, external findings of continous male attraction, continous external swelling and purulent exudate were recognized in all the experimental groups and the bitches in diestrus showed higher value of the findings than the bitches in anestrus. Of the changes of genital organs after the hormone treatment, hypertrophy of uterine horn, sanguineous exudate and purulent exudate as uterine findings were recognized in all the groups and these findings were shown more in the bitches in diestrus than in those in anestrus. These results indicated that group C showed the highest value of all the experimental groups in external signs of estrus and estrus detection and also pregnancy was recognized only in that group, consequently, that the hormonal treatment of group C would be the most effective for estrus induction, and also indicated that bitches in anestrus were more suitable than bitches in diestrus for the induction of estrus. In addition, side effects in external genital organs and uteri after hormone treatment were shown more in the bitches in diestrus than in those in anestrus, indicating that bitches in anestrus would be of choice for estrus induction.