Evaluation of Toxicity of Green Tea Extract in Chilled Boar Spermatozoa

다국어 초록 (Multilingual Abstract)

The cold shock of spermatozoa is associated with oxidative stress induced by reactive oxygen species. This studywas conducted to evaluate the toxicity of natural antioxidant green tea extract (GTE) in lactose-egg yolk (LEY) extenderduring boar sperm cooling prior to freezing. Spermatozoa were cooled to 5℃ for 3 h in LEY extender containing0 (control), 1, 10, 100 or 1,000 mg/l of GTE, re-suspended with LEY-glycerol-Equex extender and cooled at 5℃ for30 min. Sperm progressive motility, viability and phosphatidylserine (PS) translocation were evaluated. PS translocation wasassayed by flow cytometry using Annexin V-FITC apoptosis detection kit. The sperm function including progressivemotility, viability and PS translocation was not significantly different regardless of GTE concentrations (P>0.05). Inconclusion, this study demonstrated non-toxicity of GTE supplement in LEY extender during sperm cooling.

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