Methylation Status and Expression of E-cadherin in Oral Squamous Cell Carcinomas Compared to Benign Oral Epithelial Lesions

Son, Hyun-Jin,Chu, Jung-Youb,Cho, Eui-Sic,Lee, Dong-Geun,Min, Myung-Gee,Lee, Suk-Keun,Cho, Nam-Pyo The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Expression of invasion/metastasis suppressor, E-cadherin, is reduced in many types of human carcinomas. Although somatic and germline utations in the CDH1, which encodes the human E-cadherin, have frequently been reported in cases with diffuse gastric and lobular breast ancers, irreversible genetic inactivations are rare in other human carcinomas. Recently, it has been well documented that some genes in human cancers may be inactivated by altered CpG methylation. Herein, we determined the expression and methylation status of E-cadherin in oral squamous cell carcinoma (SCC) by immunohistochemistry and methylation-specific PCR. The expression of E-cadherin was significantly higher in the well-differentiated oral SCCs than the moderately or poorly differentiated ones. None of eight tested benign pithelial hyperplasias showed aberrant methylation, whereas five of 12 oral squamous cell carcinomas showed aberrant methylation. When we compared E-cadherin expression with methylation status, oral SCCs with normal methylation showed a higher expression of E-cadherin than those with methylation. These findings suggest that aberrant CpG methylation of CDH1 promoter region is closely associated with transcriptional inactivation and might be involved in tumor progression of the oral mucosa.

물-공기 계면에서 긴 사슬 양 말단 작용기 분자인 α,ω-13,16-Dimethyloctacosanedioate Dimethylester(C_30DME)의 거동에 관한 연구

이정근,손대원,이관수,송석호,김현명,정선호 한양대학교 자연과학연구소 2001 自然科學論文集 Vol.20 No.-

물-공기 계면에서 표면압력의 증가에 따른 C30DME 분자의 구조 변화를 살펴보았다. 표면압력(π)-표면적(A) 등온선을 통해 표면적이 줄어들 때 C30DME 분자가 선형으로부터 역 U 자형 구조로 변하는 것을 확인하였다. C30DME의 역 U 자형 구조의 형성을 확인하기 위하여 Surface Plasmon Resonance (SPR)와 ellipsometry를 이용하여 Langmuir-Blodgett (LB) film 의 두께를 측정하였고 이 두께는 C30DME 분자의 역 U 자형 구조의 높이와 일치함을 관찰하였다. 또한 Atomic Force Microscope (AFM)을 이용한 표면 거침도 측정의 결과는 선형으로부터 역 U자형 구조로의 구조적 변화가 불연속이라는 것을 보여주었다. We investigated the conformational change of C30DME molecule with an increase on surface pressure at the air-water interface. The surface pressure (π)-area (A) isotherm indicates that the structure of C30DME changed from linear to reverse U-shape as the surface area was compressed. In order to confirm the formation of reverse U-shape, we examined the thickness of Langmuir-Blodgett (LB) film by a surface plasmon resonance (SPR) and an ellipsometry. The result of surface roughness measurement using an atomic force microscope (AFM) showed that the conformational change from linear to reverse U-shape is discontinuous.

효율적인 소규모 네트워크 구축

안봉근,이병수,정현식 한국경영과학회 1995 經營 科學 Vol.12 No.3

Information sharing and automated office in the use of PC necessitate corporate network to implement business innovation. Building an efficient network requires competent knowledge on capabilities of various LAN technologies, hardwares and softwares that have rapidly improved in terms of efficiency and cost. This paper compares capabilities of network components: communication protocol, hardware, network operation system, low cost database software and mid-range priced SQL for application development. Also this study presents a new technology on virtual LAN and a selection guide among the various options of the network components which are suitable for PC-based small-sized network in balance of efficiency with economy. In the last section of this paper, a case of small-sized network is given for illustration.

개발에 의해 조성된 매립지의 기후특성(Ⅱ)

도근영,김가현,이승휘,옥치율,강대석 東亞大學校 海洋資源硏究所 2003 硏究論文集 Vol.15 No.-

This study started with the measured experiment of climatic characteristic at the coastal reclamation area formed by the reclamation and development which is inevitable selection presently. As a result of the examination, the climate variation is predicted to be large in summer and the salt concentration is shown in the order of top layer, bottom layer and intermediate layer. Therefore, the greens space and wind path are occupied at the coastal reclamation.

Global-MAHN에서 라우팅 오버헤드 개선에 대한 연구

張良根,金玄旭,李光培,吳世德,裵振勝,金在浩 明知大學校 産業技術硏究所 2007 産業技術硏究所論文集 Vol.26 No.-

In this Paper, we studied the mechanism that increases the network reliability and decreases network load in GMAHN environment based on the AODV routing protocol. This proposed algorithm is the research on the algorithm, minimizing network overhead by preventing unnecessary network overhead previously and adapting Local repair mechanism to efficient data transmission when route error happens in network, in GMAHN environment. To evaluate the proposed algorithm's performance, we analyzed the proposed algorithm and adapting in various experiment environment according to 5 experiment viewpoint such as node movement, gateway network zone, mobile node number, flow number. After evaluating the experiment, we confirmed better network improvement altogether through following result such as total average data reception rate's increase, average data transmission time's decrease, network overhead's decrease than existing GMAHN algorithm.

무의 PCR에 영향을 주는 요인에 관한 실험

김현주,최근원 경희대학교 부설 식량자원개발연구소 1994 硏究論文集 Vol.15 No.-

무 품종들에 있어 RAPD 분석을 위한 PCR에 영향을 미치는 요인들을 조사하기 위한 일련의 실험이 실시되었으며, 주요결과를 요약하면 아래와 같다.; 1. 무 '알타리 01'×'알타리 13'에 있어 RAPD 분석을 위한 PCR 반응 요인의 적정 농도 또는 양은 50 ㎕의 반응용액내에 50 ng의 template DNA, 15 pM의 10-mer primer, 0.2 mM의 dNTP와 0.25 unit의 Taq DNA polymerase로 사료되어졌다. 2. 무 '알타리 13'×'서울봄무'에 있어 RAPD 분석을 위한 PCR 반응 요인의 적정 농도 또는 양은 50 ㎕의 반응용액내에 50 ng의 template DNA, 10 pM의 10-mer primer, 0.2 mM의 dNTP와 0.25 unit의 Taq DNA polymerase로 사료되어졌다. 3. 38℃의 annealing 온도가 공시된 무의 PCR에 적합하였다. 4. 반응 요인들의 농도와 양에서 나타난 결과로 보아 그들의 효과에 있어 각각은 상호의존적인 관계에 있는것으로 나타났으며, 반응요인의 적정 조건들은 채소작물에 따라 매우 다양한 것으로 나타났다. A series of experiments were conducted to examine parameters affecting Polymerase Chain Reaction in studying Random Amplified Polymorphic DNA of radishes. The major results were summarized as follows; 1. In 'Altari 01'×'Altari 15', optimum concentrations or amounts of reaction parameters for RAPD analysis were found to be 50ng of template DNA, 15pM of 10-mer primer, 0.2nM of dNTP, and 0.25 units of Taq DNA Polymerase in 50㎕ of reaction mixture. 2. In 'Altari 13'×'Seoul Bom Moo', optimum concentrations or amounts of reaction parameters for RAPD analysis were found to be 50ng of template DNA, 10pM of 10-mer primer, 0.2mM of dNTP, and 0.25 units of Taq DNA polymerase in 50㎕ of reaction mixture. 3. The annealing temperature of 38℃ was optimum for PCRs of radish tested. 4. The results suggested that the concentrations or the amounts of reaction parameters were correlated with each other in their effects on amplification and showed that optimum conditions of reaction parameters could very depending on vegetable crops.

Nondestructive Characterization of Metal Matrix Composite by Means of Ultrasonics

한근조,이준현 東亞大學校 海洋資源硏究所 1993 硏究論文集 Vol.6 No.1

초음파를 이용하여 비파괴적으로 단섬유 강화 알루미늄복합재료의 탄성계수의 측정기법에 대하여 연구하였다. 이를 위해 강화섬유 함유량을 0%에서 30%까지 5단계로 변화시킨 시험편을 제조하여 강화재 체적율의 변화에 따른 탄성계수의 변화를 정량적으로 측정하여 종래의 파괴적인 방법으로 구한 결과와 비교 검토 하였다. 그 결과 초음파의 종파, 횡파속도로 부터 비파괴적으로 평가된 탄성계수의 값은 4점굽힘시험에 의해 파괴적으로 측정된 값에 비교해 약간 낮은 값을 보이고 있으며, 등가응력조건(equal stress condition) 식에서 구한 이론적인 결과와 잘 일치하고 있음을 보여주고 있다.

실험 방광암 모델에 있어서 Proliferating Cell Nuclear Antigen이 발현되는 세포들의 색상-화상 분석

이현무,이태수,김원재,차은종,송형근 충북대학교 의과대학 충북대학교 의학연구소 1993 忠北醫大學術誌 Vol.3 No.1

Proliferating cell nuclear antigen(PCNA)에 대한 단클론성 항체에 의해 면역조직화학적으로 염색된 세포들의 평가에 있어서 색상-화상 분석의 역할을 살펴보고자 하였다. BBN 투여 기간에 따라 Fisher 344 백서 방광에 정상 상피로부터 단순 과증식, 결절성 혹은 유두상 과증식 및 표재성 방광암에 이르는 순차적인 병리세포학적 변화가 발생함을 관찰한 후, 이를 색상-화상 분석과 육안적 분석을 통해 PCNA에 대한 평균 labeling index를 구한 결과 두가지 방법에서 모두 각 병변 사이에 통계적으로 유의한 차이를 보였으나, 색상-화상 분석을 이용하여 구한 평균 labeling index의 변이계수가 육안적 분석을 이용하여 구한 평균 labeling index의 변이계수에 비해 전 병변에 대하여 낮게 나타나, 종양화과정이 진행함에 따라 PCNA에 대한 평균 labeling index가 증가하고 평균 labeling index가 높을 수록 증식도가 더 큼을 나타낸다는 결과를 얻었으며, 색상-화상 분석을 이용하여 얻은 결과가 육안적 분석을 이용하여 얻은 결과보다 신뢰도가 더 높은 것으로 나타났다. 이와같은 면역조직화학염색에 대한 색상-화상 분석을 통한 세포역동학적 연구는 정량화 및 재현성의 향상을 통해 면역조직화학염색의 평가에 있어 보다 신뢰도가 높은 정보를 제공하여줄 것이며, 이를 통해 방광종양의 증식도 및 악성도를 예측하는데 중요한 역할을 할 것으로 생각된다. A study was carried out to define the role of color-image analysis in evaluation of immunohistochemically labeled cells using anti-PCNA/cyclin monoclonal antibody. Sequential cellular changes(normal, simple hyperplasia, nodular or papillary hyperplasia, and transitional cell carcinoma-Ta, T1) were observed in Fisher 344 rat urinary bladder depending on the duration of BBN administration. The mean PCNA labeling index obtained by color-image analysis and visual analysis in normal, simple hyperplasia, nodular or papillary hyperplasia and bladder tumor showed statistically significant difference in each histological group. But coefficients of variation of mean PCNA labeling index obtained by color-image analysis were lower than those using visual analysis in all lesions. Higher PCNA labeling indices indicated greater biological malignant potential, and mean labeling index in each histological group obtained by both color-image and visual analysis progressively increased with tumorigenesis, but the coefficient of variation with color-image analysis was lower than that with visual analysis in general, therefore, the evaluation of labeling indices obtained using color-image analysis was more reliable than that obtained using visual analysis. The data suggest that cell kinetic evaluation using color-image analysis may provide more reliable information to evaluate immunohistochemical staining in terms of quantitation and reproducibility and play an important role in predicting proliferative activity and malignant potential for bladder tumor.

고추 PCR 조건의 최적화

최근원,이승우,남승현,설일환,유일웅 경희대학교 부설 식량자원개발연구소 1996 硏究論文集 Vol.17 No.-

신품종에 대한 육종가의 권리보호용 고추 RAPD 마커를 개발하기 위한 기초연구로 PCR에 영향을 주는 요인들에 대한 최적화가 이루어졌으며 주요 결과를 요약하면 다음가 같다. 1. MgCl₂ 농도는 DNA증폭에 직접적인 영향을 주었으며 본 실험의 결과는 고추 PCR에 요구되는 MgCl₂의 최소농도는 3mM임을 보였다. 2. 공시된 2고추 조합간데 차이는 있었지만 Taq DNA polymerase는 1.5U이상의 조건일 때 DNA증폭에 효과적임이 관측되었다. 3. 본 실험에 사용된 template DNA양 중 최소인 10ng의 수준으로도 성공적인 DNA증폭을 보였다 4. dNTPs농도는 200~400μM 수준이 적당하였다. 5. Primer의 농도가 높아짐에 따라 band의 선명도가 증가하는 경향을 보였으며 200nM이상의 농도에서 뚜렷한 band가 관측되었다. 6. 공시된 두조합에서 모두 annealing 온도가 높아짐에 따라 minor band의 수가 줄어들고 major band의 선명도가 증가했다. 7. 본 실험의 결과는 3mM의 MgCl₂, 1.5U의 Taq, DNA polymerase, 10ng의 template DNA, 200μM의 dNTPs, 200nM의 primer, 그리고 42℃의 annealing 온도가 고추의 random primer를 이용한 PCR이 적정수준이 될 수 있음을 제시하였다. Factors affecting PCR have been optimized as a fundamental study in developing RAPD markers to protect breeder's right for new cultivars in Capsicums. DNA amplification was directly influenced by MgCl₂ concentration and the result showed that the minimum concentration required for PCR in Capsicums was 3mM. Although there was a difference between two pepper combinations tested, it was observed that over 1.5U of Taq. DNA polymerase was effective in DNA amplification. A successful DNA amplification was obtained with 10ng of template DNA, the smallest amount tested in this experiment. The proper level of dNTPs was the range between 200 and 400μM. There was a tendency that the intensity of bands was increased as the concentration of random primer increased and clear bands were observed with the concentration above 200nM. In both pepper combinations tested, the number of nonspecific bands was decreased and the intensity of major bands was increased as the annealing temperature was increased. The results suggested that the optimum conditions for PCR with random primer in Capsicums could be obtained with 3mM of MgCl₂, 1.5U of Taq. DNA polymerase, 10ng of template DNA, 200μM of dNTPs, 200nM of random primer, and 42℃ of annealing temperature.

멀티미디어 데이타를 위한 E-mail 시스템의 설계 및 구현

金昌根,朴淳圭,金炫周 진주산업대학교 1998 산업과학기술연구소보 Vol.- No.5

Even though UNIX system has E-mail function, but it confined only Text-data, RFC 821/822 the standard of internet mail also confined 7bit Text-data, there is still limit in displaying various multimedia infomation. In this paper, java was used to complete the practical electronic mail system over various Operation System, MINE was standarded to support various media, SMTP and POP3 were set for design and implementation. Therefore, Electronic Mail system more it possible to be used in Personal Computer.