DNA 수준에서 한국인 HLA-Class Ⅰ 대립유전자형 및 일배체형 분포 : the Molecular Basis

최희백,김형재,김태규,김창규,정태준,한훈 대한조혈모세포이식학회 1997 대한조혈모세포이식학회지 Vol.2 No.1

The products of the human leukocyte antigen (HLA) have been detected by the serological or cellular methods. With the evailability of DNA sequences for alleles of the HLA system, and with the development of molecular biological techniques it has been possible to define the genotypes in HLA genes. And the amplification of DNA using sequence-specific primers has been proved as a reliable and rapid method for typing of HLA class II genes. We studied the distribution of the HLA-A, -B, C genotypes on 114 unrelated individuals by amplification refractory mutation system-polymerase chain reaction (ARMs-PCR). 13, 24 and 14 alleles in HLA-A, B and C genes were detected in normal Korean. The genotypes showing frequencies more than 18 percent were A*02(65.8%), A*24(02, 03) (41.2%), A*33(01, 02) (21.1%), A*11(01, 02) (20.2%), B*15(01, 02, 03, 04, 05)/B52(011, 012) (21.9%), B815(01, 04, 05, 06, 07, 12, 19, 20) (19.3%), B*40(02, 04, 05, 06) (18.4%), Cw*08(01, 02, 03) (28.9%), Cw*0303(27.2%), Cw*0304(26.3%) and Cw*01(01, 02) (24.6%). And most common 2-loci hyplotypes with frequencies larger than 0.04 were A*02-B*15(02, 08, 11, 15) (HF: 0.045), A*24(02, 03)-B*51(01, 02, 03, 04, 05)/*52(011, 012)(HF:0.044), B*51(01, 02, 03, 04, 05)/*52 (011, 012)-Cw14(01, 02) (HF: 0.069). These results suggest that the DNA typing of HLA class I may be an efficient typing method compared with the conventional method.

약물전달체를 위한 폴리에틸렌글리콜과 폴리카프로락톤을 이용한 나노입자

김태형,채수영,최창용,김동곤,김명열,나재운 한국공업화학회 2004 응용화학 Vol.8 No.2

The self-aggregation behavior and microscopic characteristics of the PEG-b-PCL copolymer self-aggregates, prepared by solvent evaporation method, were investigated by `H NMR, dynamic light scattering (DLS), and fluorescence spectroscopy. The PEG-PCL block copolymer formed nano-sized self-aggregates in an aqueous environment by intraand/or intermolecular association between hydrophobic PCL chains. Then, drug loading and its release behavior of clonazepam loaded PEG-b-PCL nanoparticles were investigated. The result revealed that hydrophobic-hydrophilic balances played a crucial role for drug loading and its release behaviors.

Preparation and Characterizations of Poly(ethylene glycol)-Poly(ε-caprolactone) Block Copolymer Nanoparticles

Choi, Chang-Yong,Chae, Su-Young,Kim, Tai-Hyoung,Jang, Mi-Kyeong,Cho, Chong-Su,Nah, Jae-Woon Korean Chemical Society 2005 Bulletin of the Korean Chemical Society Vol.26 No.4

Diblock copolymers with different poly($\varepsilon$-caprolactone) (PCL) block lengths were synthesized by ringopening polymerization of $\varepsilon$-caprolactone in the presence of monomethoxy poly(ethylene glycol) (mPEG-OH, MW 2000) as initiator. The self-aggregation behaviors of the diblock copolymer nanoparticle, prepared by the diafiltration method, were investigated by using $^1H$ NMR, dynamic light scattering (DLS), and fluorescence spectroscopy. The PEG-PCL block copolymers formed the nano-sized self-aggregate in an aqueous environment by intrsa- and/or intermolecular association between hydrophobic PCL chains. The critical aggregation concentrations (cac) of the block copolymer self-aggregate became lower with increasing hydrophobic PCL block length. On the other hand, reverse trends of mean hydrodynamic diameters were measured by DLS owing to the increasing bulkiness of the hydrophobic chains and hydrophobic interaction between the PCL microdomains. The hydrodynamic diameters of the block copolymer nanoparticles, measured by DLS, were in the range of 65-270 nm. Furthermore, the size of the nanoparticles was scarcely affected by the concentration of the block copolymers in the range of 0.125-5 mg/mL owing to the negligible interparticular aggregation between the self-aggregated nanoparticles. Considered with the fairly low cac and nanoparticle stability, the PEG-PCL nanoparticles can be considered a potential candidate for biomedical applications such as drug carrier or imaging agent.

Aircraft Longitudinal Auto-landing Guidance Law Using Time Delay Control Scheme

CHOI, Hyoung Sik,LEE, Sangjong,LEE, Jangho,KIM, Eung Tai,SHIM, Hyunchul THE JAPAN SOCIETY FOR AERONAUTICAL AND SPACE SCIEN 2010 Transactions of the Japan Society for Aeronautical Vol.53 No.181

<P>The objective of this paper is applying the Time Delay Control scheme for the aircraft auto-landing guidance problem. A stability and control augmentation system and longitudinal auto-landing guidance law, using Time Delay Control, is proposed and evaluated through a simulation with model uncertainties and wind disturbances. The proposed Time Delay Control guidance law shows good performance and is robust to model uncertainties and disturbances.</P>

Characterization of Nanoparticles Prepared by poly(ethylene glycol)-block-poly(ε-caprolactone)

Kim, Tai-Hyoung,Chae, Su Young,Choi, Changyong,Chae, Hey Young,Kim, Dong-Gon,Kwon, Joong-Keun,Nah, Jea-Woon 한국공업화학회 2004 응용화학 Vol.8 No.1

Amphiphilic diblock copolymer with different poly(ε-caprolactone) (PCL) block length were synthesized by ring-opening polymerization of ε-caprolactone in the presence of monomethoxy poly(ethylene glycol) (mPEG-OH, MW 2000) as initiator, using stannous octoate catalyst. The self-aggregation behavior and microscopic characteristics of the diblock copolymer self-aggregation behavior and microscopic characteristics of the diblock copolymer self-aggregates, prepared by dialysis method, were investigated by ¹H NMR, dynamic light scattering (DLS), fluorescence spectroscopy. The PEG-PCL block copolymer formed self-aggregates in an aqueous environment by intra- and/or intermolecular association between hydrophobic PCL chains. The microscopic chacaterization of the PEGCL self-aggregates revealed that the hydrophobic/hydrophilic balance, resulting from different PCL block length, played a crueial roles fo ditermination of the physicochemical properties of them.

유한골접산이 백서의 골절 치유과정에 미치는 영향

김태승,김원규,황규성,최윤영,이관형,최일용,성일훈 대한골절학회 2000 대한골절학회지 Vol.13 No.4

Objectives: We have studied the effects of Yuhan bone connecting powder on the healing process of the frauture at the osteotomized bones in rat, using bone densitometer, X-ray and morphological studies after administration of Yuhan bone connecting powder which has been reported in China as enhancing the processes of the fracture healing. Materials and Methods: 90 Sprague-Dawley male rats were divided into the rib and tibia osteotomy groups. Each group was subdivided into the control, low dosage and high dosage groups. Rib and tibia were osteotomized under the general anesthesia with ketamin. From the next day after operation, Yuhan bone connecting powder diluted with distilled water was administrated to low(0.68g/kg) and high(1.36g/kg) dosage groups per se. Same amount of the distilled water was used to the control group. 1.5cm long rib including the osteotomy site and whole tibia were excised. Bone densitometer using dual energy X-ray absorptiometry, radiological and morphological studies with HE stain and alcian blue(pH 2.5)-PAS stain were performed. Results: BMD showed statistically significant difference between control group, low dose group and high dose group at 2weeks after treatment(p=0.035), but did not show such a good result at 1week(p=ns) and 4weeks(p=0.091) after treatment. Radiologically, after treatment for 2 weeks, the low and high dose groups showed more active callus formation than control group. Morphologically, dilated numerous blood vessels adjacent the bony trabeculae and well developed cartilagenous callus were observed in the experimental group at the 1st week. At the 2nd week, many newly formed bony trabeculae were formed from the cartilagenous callus, and at the 4th week relatively thick compact bone and bony trabeculae were connecting the both osteotomy ends. Conclusion: BMD at the osteotomy site seemed to be increased after administration with Yuhan bone connecting powder to rats, and this finding was supported radiologically at 2weeks after treatment. Morphologically, at the early stage of the fracture healing, numerous dilated blood vessels were distributed and many bony trabeculae were formed from the cartilagenous callus. At the remodelling stage relatively thick compact bone was connecting the both ends of the osteotomy site. So it is suggested that Yuhan bone connecting powder would induce enhancing the healing process of the osteotomized bone in rat through active vascularization, mineralization of the cartilage matrix, endochondral ossification and remodelling.

Theoretical Study on Hydrophobicity of Amino Acids by the Solvation Free Energy Density Model

Kim, Jun-Hyoung,Nam, Ky-Youb,Cho, Kwang-Hwi,Choi, Seung-Hoon,Noh, Jae-Sung,No, Kyoung-Tai Korean Chemical Society 2003 Bulletin of the Korean Chemical Society Vol.24 No.12

In order to characterize the hydrophobic parameters of N-acetyl amino acid amides in 1-octanol/water, a theoretical calculation was carried out using a solvation free energy density model. The hydrophobicity parameters of the molecules are obtained with the consideration of the solvation free energy over the solvent volume surrounding the solute, using a grid model. Our method can account for the solvent accessible surface area of the molecules according to conformational variations. Through a comparison of the hydrophobicity of our calculation and that of other experimental/theoretical works, the solvation free energy density model is proven to be a useful tool for the evaluation of the hydrophobicity of amino acids and peptides. In order to evaluate the solvation free energy density model as a method of calculating the activity of drugs using the hydrophobicity of its building blocks, the contracture of Bradykinin potentiating pentapeptide was also predicted from the hydrophobicity of each residue. The solvation free energy density model can be used to employ descriptors for the prediction of peptide activities in drug discovery, as well as to calculate the hydrophobicity of amino acids.

Time Delay Fault Tolerant Controller for Actuator Failures during Aircraft Autolanding

LEE, Jangho,CHOI, Hyoung Sik,LEE, Sangjong,KIM, Eung Tai,SHIN, Dongho THE JAPAN SOCIETY FOR AERONAUTICAL AND SPACE SCIEN 2012 Transactions of the Japan Society for Aeronautical Vol.55 No.3

<P>A time delay control methodology is adopted to cope with degraded control performance due to control surface damage of unmanned aerial vehicles, especially in the case of the automatic landing phase. It is a crucial challenge to maintain consistent control performance even under fault environments such as stuck and/or incipient actuator faults. Flight control systems designed using conventional feedback control methods in such cases may result in unsatisfactory performance, and even worse, may not guarantee the closed-loop stability, which is fatal for aircraft in the state of auto-landing. To overcome the shortfalls of the conventional approach, the time delay control scheme is adopted. This scheme is known to be robust against disturbance, model uncertainties and so on. Motivated by the fact that the abrupt and/or incipient actuator faults focused on in this paper could be considered as model uncertainties, we consider the application of the time delay controller to designing a fault tolerant control system. To show the effectiveness of the time delay control method, a nonlinear 6-DOF simulation is performed under model uncertainties and wind disturbances, and control performance is compared with that of conventional controllers in the case of multiple and single actuator faults.</P>

Fluconazole in the Treatment of Oropharyngeal Candidiasis in Pediatric Leukemic Patients

Kook, Hoon,Choi, Young Kwon,Ko, Dong Hoon,Kim, Byoung Joo,Hwang, Tai Ju,Kim, Hyoung Joon,Choi, In Sun Chonnam National UniversityResearch Institute of 1993 Chonnam journal of medical sciences Vol.6 No.1

The <i>HLA-C*07:478</i> allele identified in a volunteer donor for hematopoietic stem cell transplant

Baek, In-Cheol,Choi, Eun-Jeong,Shin, Dong-Hwan,Kim, Hyoung-Jae,Kim, Tai-Gyu Wiley (Blackwell Publishing) 2018 Hla Vol.92 No.4

<P>HLA-C*07:478 differs from HLA-C*07:02:01:01 in codon 81 in exon 2.</P>