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Kwon, Hyoung Joo,Lee, Hee Gu,Kim, Hee Sue,Choe, In-Seong,Chung, Tai-Wha,Kim, Seung-Ho,Chung, Kyeong-Soo,Kim, Kilhyoun 충남대학교 약학대학 의약품개발연구소 1992 藥學論文集 Vol.8 No.-
β-수용체 효능약물((-)-NE), 길항약물 ((±)-propranolol, labetalol) 및 PDE 억제약물 (imazodan, KR-30045, KR-30075 등)에 대한 β-adrenoceptor binding 실험을 β_1/β_2 비선택적 radioligand인 (-)-[^3H]-DHA를 사용하여 실시하였다. Saturation 실험에서 β_1 및 β_2 수용체를 모두 갖고 있는 rat 좌심실의 β 수용체에 대한 (-)-[^3H]-DHA의 K_d 값은 1.5±0.43 nM, B_max는 22.0±0.9 fmol/㎎ protein 이었다. (±)propranolol, labetalol 및 (-)NE는 단일상으로 (-)-[^3H]-DHA의 결합을 억제하였으며 Ki 값은 각각 17.0±0.43 nM, 5.73±1.30nM, 1.57±0.95μM로 나타났다. 실험에 사용한 모든 PDE 억제약물들은 (-)-[^3H]-DHA 결합을 10^3 M의 고농도에서도 10%미만으로 억제했다. 실험결과, propraolol, labetalol 및 NE는 β_1/β_2 수용체에 대해 비선택적인 약물로 나타났으며, imazodan 및 산합성 PDE 억제약물들은 rat 심근에 있는 β- 수용체에 친화성이 거의 없음을 알수 있었다. Binding of the antigenic peptide resulting from antigen processing to major histocompatibility complex (MHC) molecules constitutes a major event in T cell recognition of antigens. When they were incubated with potential antigen-presenting cells (APC), synthetic peptides bound directly to the surface of APC. The binding was specific for MHC molecules since it was substantially reduced by treating with anti-MHC antibodies. Furthemore, it was possible to render the peptides to bind only to the cell surface, by treating the APC with glutaraldehyde to block nonspecific internalization of the peptides. It was also shown that a peptide that elicited relevant immune responses and thus seemed to have T cell epitope(s) indeed bound to relavent APC surface through MHC molecules. The peptide, when analyzed theoretically by using an algorithm that had been designed for the prediction of T cell epitope(s), also appeared to have a potential T cell epitope. These results support that peptide binding assay employing whole cells as APC could provide a convenient way to determine potential T cell epitopes among a deiverse array of peptides.
( Jae Hyoung Song ),( Sang Gu Yeo ),( Eun Hye Hong ),( Bo Ra Lee ),( Jin Won Kim ),( Jeong Hoon Kim ),( Hyeon Gun Jeong ),( Yong Soo Kwon ),( Hyun Pyo Kim ),( Sang Won Lee ),( Jae Hak Park ),( Hyun Je 한국응용약물학회 2014 Biomolecules & Therapeutics(구 응용약물학회지) Vol.22 No.1
Enterovirus 71 (EV71) is the predominant cause of hand, foot and mouth disease (HFMD). The antiviral activity of hederasaponin B from Hedera helix against EV71 subgenotypes C3 and C4a was evaluated in vero cells. In the current study, the antiviral activity of hederasaponin B against EV71 C3 and C4a was determined by cytopathic effect (CPE) reduction method and western blot assay. Our results demonstrated that hederasaponin B and 30% ethanol extract of Hedera helix containing hederasaponin B showed significant antiviral activity against EV71 subgenotypes C3 and C4a by reducing the formation of a visible CPE. Hederasaponin B also inhibited the viral VP2 protein expression, suggesting the inhibition of viral capsid protein synthesis.These results suggest that hederasaponin B and Hedera helix extract containing hederasaponin B can be novel drug candidates with broad-spectrum antiviral activity against various subgenotypes of EV71.
성인에서 고인슐린혈증성 저혈당을 보인 췌장내의 미만성 Nesidioblastosis 1예
조정구,채권묵,서검석,윤기중,양광수,이승하,형근영,신봉주 대한내분비학회 1996 Endocrinology and metabolism Vol.11 No.2
Nesidioblastosis is a term that describes multifocal hyperplasia of all panereatic cell components and is characterized primarily by their disorganization and proliferation throughout the entire pancreas. Adult onset nesidioblastosis is an extremely rare entity associated with hypersecretion of insulin. The authors have recently experienced a case of nesidioblastosis in an adult. A 41-year old man was admitted due to intermittent hypoglycemic symptoms, that had been relieved by carbohydrate ingestion. Hyperinsulinemic hypoglycemia was documented during prolonged fast. Under the presumptive diagnosis of insulinoma, abdominal CT, celiac angiogram and percutaneous transhepatic portal venous sampling were done but we could not find any definitive mass. Eight-five percent of the pancreas was removed. Pathologic examination of the resected pancreas revealed irregularly sized islets and scattering of small endocrine cell clusters throughout the acinar tissue and ductuloinsular complex(1 Kor Soc Endocrinol 11:247~253, 1996).