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Hyeong-Wook Jo(조형욱) 한국농약과학회 2022 한국농약과학회 학술발표대회 논문집 Vol.2022 No.11
The number of chemical substances resgistered Cas No. is about 190 million in 2021, and there are about 397,000 types of regulated chemicals. If the types of unregulated substances that are difficult to estimate from actual statistics are included, the number of chemical substances in products that need to be monitored are very vast. The low-resolution mass spectrometry (LRMS) currently used for monitoring in food is less useful for detecting unknown residues and unregulated substances without prior information. Also, it is possible to obtain a standard material or related materials to perform a qualitative analysis, and there is a difficulty in tracking only the observed mass of an unknown residual materials. Therefore, there is a need for an analytical method that can simultaneously screening various chemical substances that may potentially remain in food even in a situation where a standard material is not secured. For such monitoring, an approach through a non-target analysis is much more advantageous than a conventional target analysis. For this reason, the use of high-resolution mass spectrometry (HRMS) has been attracting attention for non-target analysis, and non-target analysis has been applied for the safety management of agricultural, livestock and fishery products.
Simultaneous determination of 13 mycotoxins in feedstuffs using QuEChERS extraction
Jo Hyeong-Wook,Park Min-Kyu,Heo Hyo-min,Jeon Hwang-Ju,Choi Sung-Deuk,Lee Sung-Eun,Moon Joon-Kwan 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.2
Mycotoxins are secondary metabolites produced by various fungi and are known to have a significant negative impact on human and animal health. When feedstuffs are contaminated with mycotoxins, their toxicities may be caused a variety of diseases. In this study, the residual mycotoxins in feedstuffs were analyzed using LC–MS/MS incorporated with QuEChERS extraction. Analytical method validation was performed for LOD, LOQ, linearity, and recoveries with consideration of matrix effects prior to the residual analysis. They were all reached to the accepted range of validation level. Using 39 feedstuff samples (5 g) for mycotoxin analysis, nine samples were contaminated by four major mycotoxins such as fumonisin B1 (FB1), deoxynivalenol, fumonisin B2, and zearalenone. Among them, FB1 was detected at the highest concentration as 18.0943 mg/kg. The total sum of fumonisins in 39 samples did not exceed the maximum residual level (MRL) criterion set by Korean Food and Drug Administration. Altogether, intensive management of mycotoxins in Korean feedstuffs should be implemented with proper and routine monitoring, even their residual concentrations are not exceeded over the MRL levels because of high frequent detection found in this study.
GC-MS/MS를 활용한 담수 및 담수어에서의 Chloroxylenol(PCMX) 분석을 위한 분석기기 검토
조형욱(Hyeong-Wook Jo),허효민(Hyo-Min Heo),원은지(Eun-Ji Won),신경훈(Kyoung-Hoon Shin),문준관(Joon-Kwan Moon) 환경독성보건학회 2021 한국독성학회 심포지움 및 학술발표회 Vol.2021 No.5
Chloroxylenol(PCMX)은 화장품 원료로 사용되고 있으며, 환경 중에 여러 경로를 통하여 수생태로 이동될 가능성이 높고, 담수어류에서 생물농축 및 위해 가능성이 있으므로, 주기적인 모니터링을 위하여 담수 및 담수어류 중 분석을 위하여 분석기기를 검토하였다. 화장품 사용한도 성분 분석법 가이드라인(식품의약품안전처, 2018)에서는 HPLC-UV/VIS를 이용하여 280 nm에서 분석하는 방법으로 기기 검출한계는 0.2 mg/L이며 분석법 정량한계는 50 mg/kg였다. 하지만 담수 및 담수어에서 생물농축 등을 위한 모니터링을 위한 분석방법으로는 부적합한 방법이다. 따라서 낮은 검출한계값을 가지며, 좀 더 낮은 농도에서의 검출을 위하여 trimethylsilyl기(시약 1) 및 butyldimethylsilyl기 (시약 2)를 이용하여 유도체화한 후 GC-MS/MS를 활용한 분석방법을 검토하였다. 질량분석기를 활용하여 분석할 경우, 매질효과를 배제할 수 없으므로, 정량을 위해 내부표준물질로 chloroxylenol-d6도 함께 검토하였다. 검토결과, 각각 기기 검출한계는 0.001mg/L 이하로 검출이 가능하며 시약 1보다 시약 2에서 감도가 더 높았다. 시약 2를 이용하여 SIM(Select Ion Monitoring) 모드 또는 MRM(Multi Reaction Monitoring) 모드 중 적합한 ion monitoring 방법을 적용하여 분석법을 확립할 예정이다.