유산균 발효유가 Streptococcus mutans의 생균수 및 biofilm 형성에 미치는 영향

신혜성,김선미,최남기,양규호,강미선 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.3

유산균이 위장관과 구강건강에 probiotics로서 긍정적인 영향을 미치고 있음이 보고되고 있다. 이에 시판되는 유산균 발효유 5종과 우유를 선택하여 우식유발 원인균인 S. mutans에 대한 증식억제 및 biofilm 형성에 미치는 영향을 평가하고, 발효유의 산도, 완충능, 산생성능, 칼슘과 인의 함량을 측정하였다. 유산균 발효유에 S. mutans를 주입한 후 생균수를 측정한 결과 모든 발효유의 %가 증가함에 따라 S. mutans 수가 감소했으나 우유에서는 변화가 없었다. 10% 유산균 발효유에 S. mutans를 주입한 후 biofilm 형성 정도를 측정한 결과 액티브 GG, 불가리스는 biofilm 형성이 감소하였고, 이오는 유의한 변화가 없었으며, 투티, 에이스, 우유는 증가하였다(P<0.05). 유산균 발효유 및 우유의 산도를 측정한 결과 이오(3.48±0.01), 투티(3.67±0.02), 에이스(3.71±0.02), 액티브 GG(4.04±0.02), 불가리스(4.19±0.02) 순으로 나타났다. 유산균 발효유의 산생성능을 평가한 결과 불가리스가 가장 높은 산생성능을 보였으며 액티브 GG, 에이스, 투티, 이오, 우유 순으로 산도 하강 경향을 나타냈다. 이상의 결과는 유산균 발효유가 초기산도 및 산생성능 측정 결과 우식유발 가능성이 있음을 나타냈다. 그러나 유산균 발효유 중에서 농후발효유가 우식유발균인 S. mutans를 억제하였고 biofilm 형성을 감소시켜 긍정적인 효과를 가지고 있음을 알 수 있었다. Lactic acid bacteria worked positively on gastrointestinal tract and oral environment. So I selected commercial five fermented milks and milk. and then I evaluated their effect of growth inhibition and biofilm formation of cariogenic bacteria. Streptococcus mutans. And also calculated the acidity. buffering capacity. concentration of Ca and P ion and pH change of those drinks. After adding S. mutans to fermented milks viable cell count of S. mutans in milk was not statistically different but those in all fermented milks were decreased as concentration of fermented milk increased. When I measured the amount of formed biofilm in 10% fermented milks and milk with S. mutans and compared with those without S. mutans. the amount was decreased in Active GG and Bulgaris while being increased in Tootee. Ace and milk(P<0.05). The fermented milk with the lowest pH value was E5(3.48±0.01). and the highest was Bulgaris(4.19±0.02). pH change of the fermented milks and milk with S. mutans was measured. The highest acid producing fermented milk was Bulgaris. and followed by Active GG, Ace, Tootee, E5, Milk. These results indicated that fermented milks had caries activity due to the value of initial acidity and acid producing capacity. But, concentrated fermented milks had the inhibitory effect against S. mutans, and also had high volume of Ca and P ion that protected teeth. So I suggest that they have positive effect on teeth.

운동을 통한 체중조절 프로그램에 의한 식이섭취량과 혈청지질 분포의 변화

신혜준,김희선 순천향대학교 기초과학연구소 2001 순천향자연과학연구 논문집 Vol.7 No.2

The purpose of this study was to investingate how regular exercise program affects on physical fitness and changes of body fat(%) and serum lipid profiles of female university students. Twelve students were recruited and 7-week exercise program was conducted at least 3 days per week for 40 minutes. The body weight and body fat changes were measured during exercise program. Changes of dietary intake and serum lipid profiles were monitored before and after excercise program. The results were as follows. 1. After regular exercise, weight and body fat(%) was reduced significantly (1.12kg,1.6%, p<0.01) 2. High-density lipoprotein concentration was significantly (p<0.01) increased and triglyceride level was reduced significantly (p<0.01) while total cholesterol and low-density lipoprotein concentration did not show significant changes after the exercise program. 3. After exercise program, better nutritional intake pattern was observed. In summary, regular excercise program showed positive effects on decrease of body weight, body fat and serum lipid profiles as well as dietary intakes.

색소실조증 환아의 치험례

신혜성,김선미,최남기,양규호 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.4

Incontinentia pigmenti(Bloch-Sulzberger syndrome) is a sex-linked hereditary disorder so girls are almost exclusively affected. The frequency rate is approximately 1:40,000 among girls. It is associated with skin, ocular, dental, skeletal and central nervous deformities. We reported 2 cases of medically diagnosed IP patients who were 4 and 5 years old girls. They had thin and sparse hair, and represented congenital missing of multiple primary and permanent teeth, accessory cusps and cone-shaped crowns. Therefore we report the dental manifestations and treatment progress. 색소실조증(Incontinentia pigmenti)은 반성우성으로 유전되며, 대부분 여아에서 발생하고 여아 4만 명 중 한 명에서 발생하는 드문 질환이다. 증상으로는 색소침착, 수포형성, 안구 증상, 치아 이상, 골격계 이상, 신경계 이상 등을 동반할 수 있다. 본 증례는 의과적으로 색소실조증으로 진단받고, 치아 결손을 주소로 내원한 5세 여아와, 4세 여아이다. 이들은 가늘고 성긴 머리카락을 나타내었으며, 구강 내 증상으로는 다수 유치와 영구치의 결손, 과잉교두, 원뿔형 치관형태 등을 나타내었다. 이에 구강 증상 및 치료 경과를 보고하는 바이다.

길랑-바레 증후군으로 초래된 기립보행불능증:증례보고

이혜림, 이상수, 이성현, 백신혜, 이형석, 신동익, 김지선 충북대학교 의과대학 충북대학교 의학연구소 2012 忠北醫大學術誌 Vol.22 No.1

기립보행불능증 (astasia-abasia)은 양호한 근력과 운동기능을 가지고 있음에도 서거나 걷는 것이 불가능한 것을 말한다. 이것은 흔히 정신적인 질환으로 간주되지만 기질적인 원인들도 보고되고 있다. 보행영역은 근육과 말초 신경 뿐 아니라 전정-소뇌-시상-피질 연결고리의 되먹임 작용에 의한 연계가 필요하다. 우리는 길랑-바레 증후군을 가진 환자에게서 나타났던 기립보행불능증을 경험하였고, 신경염 과 함께 보행 영역 연결의 붕괴가 증상 발생과 연관이 있을 것으로 사료된다.

결절종으로 초래된 후방골간신경 마비: 증례보고

신재원, 백신혜, 이형석, 신동익, 이성현, 이상수, 김지선 충북대학교 의과대학 충북대학교 의학연구소 2011 忠北醫大學術誌 Vol.21 No.2

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地衣類의 脂質과 蛋白質 含量

徐信惠,李鴻善,崔璟姬,崔正渼 효성여자대학교 가정대학 학도호국단 1984 家政大論集 Vol.3 No.-

地衣類의 영양학적 가치를 조사하기 위하여 地衣類(지붕, 바위, 나무)와 석이의 總脂質 含量, 脂質 분포, 脂肪酸 조성, 蛋白質 含量을 측정하였다. 그 결과 총지질 함량은 100g당 석이는 3.47g, 바위에 地衣類는 3.40g, 지붕의 地衣類는 3.17g, 나무의 地衣類는 2.73g 이었고 TLC法에 의한 脂質의 분포는 석이와 地衣類 모두 cholesterol ester와 phospholipid 등이 있었고 나무 地衣類에는 TG 및 cholesterol의 흔적을 보였다. 지방산 조성은 대부분 C_12~C_18 fatty acid였고 그 중 C_18 : 1 C_18 : 2인 것이 50% 정도로 높게 나타났다. 나무의 地衣類에는 C_18 이상의 fatty acid도 상당량 분포되어 있었으며 C_20.4 fatty acid는 18.9%였다. 단백질 함량은 석이 6.60g, 地衣類는 8.22g이었다. To investigate the nutritional value of lichens, contents of total lipid, compositions of fatty acids, distributions of lipids and contents of total proteins in lichens (on the rock, roof and tree) and sogi were examined. The results were summarized as follow. The contents of total lipid in sogi, rock, roof, and tree lichens were 3.47±0.05%, 3.40±0.09%, 3.17±0.02%, 2.73±0.04% respectively. Lipids in all them were cholestrol ester and phospholidpid, and TG and cholesterol were existed only the lichen on the tree. Fatty acids were composed of that of carbon number 12-18; C_18:1 and C_18:2 were about 50%, Contents of total protein in sogi and lichen (roof) were 6.60% and 8.22% respectively.

얼굴 연조직 강화를 위한 자가지방 이식 후 발생한 급성 경계부위 뇌경색

민경현, 이혜림, 여민주, 김지선, 이성현, 이상수, 신동익 충북대학교 의과대학 충북대학교 의학연구소 2016 忠北醫大學術誌 Vol.26 No.1

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Retinoic acid에 의해 유발된 흰쥐의 구개열에서 Transforming Growth Factor-beta 1, 2, 3의 발현의 변화

김종환,박진혁,신선호,윤혜경,김우형,이희철 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Objective : Craniofacial malformations are among the most frequent congenital birth defects in humans : cleft palate, that is inadequate fusion of the palatal shelves. Retinoic acid is teratogenic in many species, producing multiple malformations, including cleft palate. Also it plays an important role in embryogenesis, by regulating morphogenesis, cell proliferation, differentiation, and deposition of the extracellular matrix. The effects of retinoic acid which leads to cleft palate vary depending on the stage of development exposed. After exposure of embryonic mice to retinoic acid on gestational day(GD) 10, abnormally small palatal shelves form. And the elevation of palatal shelves is delayed. The growth and differentiation factor, transforming growth factor-beta(TGFβ) family is thought to play important roles in multiple developmental processes, especially, the development of the embryonic palate. The expression of the TGFβ family in embryonic palate suggests a functional role for these molecules. In this study, author has determined the tissue distributions of TGFβ family in murine embryonic palate, and compared them in terms of developmental stage and locations in normal and retinoic acid-induced cleft palate. Methods and Material : Retinoic acid was administered orally at gestational day(GD) 10 to ICR mice. The pregnant mice were sacrifeced on GD 12, 13. 14, 15 to obtain the fetuses and immunohistochemistry was performed. And using the scanning electron microscope, morphology of normal palate and cleft palate was studied. Results : In the retinoic acid-treated fetuses, palatal shelves did not elevate and cleft palate was induced. On GD 13 in the palates of the retinoic acid-treated fetuses, expression of the TGFβ1, 3 decreased dramatically, but that of TGFβ2, did not change. The expression of the TGFβ1, 3 in the palates of the retinoic acid-treated fetuses, increased on GD 14, 15, Significant differences in TGFβ family expression were observed with areas of the palate. Conclusion : These results suggest that retinoic acid inhibits the expression of TGFβ 1, 3, which is critical in the developing palate, and elevation of palatal shelves is delayed and impaired, therefore cleft palate is induced. These data demonstrate a role for retinoic acid and retinoic aicd-induced TGFβ family in the regulation of palatal development and suggest a role for TGFβ in retinoic acid-induced cleft palate.

환기정도에 따른 수술실용 신발 종류가 수술실 오염에 미치는 영향 : How to Choose Suitable Shoes?

남경동,정혜선,박영신,원진희,주미자,성화신,이지혜,이병희,조경숙,배재춘 한국의료QA학회 2000 한국의료질향상학회지 Vol.7 No.1

Background : Various types of protective footwear have been used to minimize bacterial contamination in operating rooms. In recent years, debate has arisen concerning the need for use of such protective footwear. This study was designed to provide useful data about choosing shoes most suitable for the surgical environment. Methods : Between November, 1999 and January, 2000, we performed this experimental study by comparing effect of three types of shoes (i.e., disposable shoescover, operating room-restricted shoes, and ordinary shoes) on bacterial contamination of operating rooms equipped with two different ventilation systems (i.e., high air-change, low air-change) respectively. Data were collected during two-hour sham operations in which subjects and their activities were strictly standardized. Bacterial flora were sampled from the study area floor and air colony-counts were measured. Results : In experiments involving high air-change ventilation system, there was a significant difference of floor contamination between three types of shoes, but no difference of air contamination. Under low air-change system, there was a significant difference of both floor and air contamination between three types of shoes. Conclusion : The results show that protective footwear would be unnecessary in the operating room with high air-change ventilation system, but it is important to choose suitable shoes carefully under low air-change system. Therefore, the use of outdoor shoes can be considered under high air-change system, but it would seem sensible to apply their first use in less bloody operations at the day surgery center or out-patient department to prevent transfer of body fluid into the outside environment.

정상교하 아동의 두개안면부 성장에 관한 종적 연구

양규호,박창헌,손정수,김낙현,최남기,김선미,김기백,신혜성 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.3

혼합치열기 정상교합 아동 24명(남:14명 여:10명 초진 시 평균 나이 9±1.3세, 평균 관찰 기간: 13±1.3개월)에 대한 성장량을 측정하여 기능적 교정장치의 순수 치료효과를 평가하는데 도움이 되기 위해 3회(5~8개월 간격) 촬영한 측모 두부 방사선 규격 사진에 대한 분석 결과 다음과 같은 결론을 얻었다. 1. 남아는 상악골은 전하방, 하악골은 전방성장하였고, 여아는 상하악골이 전하방 성장하였다(p<0.05). 2. 상하악골의 남녀간 차이에서 수평적 성장상태는 여아가 컸고(A point 여아: 2.39mm, 남아: 1.26mm, p<0.05), 수직적 성장상태는 유의한 차이가 없었다. 3. 상악 전치의 치축은 두개저에 대해서 순측 경사하였고(p<0.01) 하악 전치의 치축은 큰 변화가 없었다. The purpose of this study was to provide the reference data evaluating the treatment effect of orthopedic appliances. The skeletal and dental growth increments were measured in 24 normal mixed dentition children(boys: 14, girls: 10) by three serial lateral cephalograms: initial mean age: 9±1.3 years, mean observation period: 13±1.3 months, Cephalometric changes were analysed. The results were as follows: 1. In boys, the maxilla showed forward and downward growth pattern and the mandible showed forward growth pattern(p<0.05). In girls, the maxilla and mandible showed forward and downward growth pattern(p<0.05). 2. Horizontal growth of both maxilla and mandible in girls was superior to those in boys(A point: girls: 2.39mm, boys: 1.26mm, with p<0.05), whereas vertical growth of both maxilla and mandible in boys was similar to those in girls. 3. The change in tooth axis showed labioversion of upper incisor(p<0.01) and comparatively stable lower incisor position.