Oxidative Stress for the Stroke

Hyangkyu Lee,Younghwa Kim 한국생활환경학회 2014 한국생활환경학회지 Vol.21 No.4

The aim of this study is to provide fundamental knowledge for the mechanism of oxidative stress in stroke. The collection of literatures in this study was performed through a biomedical database, Pubmed, Korean research information service system, Riss4U, a science and technical database, ScienceDirect and Internet. This study investigated the known mechanisms of oxidative stress in stroke through published literature reviews. Stroke is associated with the production of reactive oxygen species (ROS) after ischemia-reperfusion in prehospital emergency system, and the use of many antioxidants is being considered in order to reduce the pathological damage caused by ROS. To enhance the quality of prehospital practice, the emergency medical technicians need to understand the accurate mechanism of oxidative stress, identify the risk factors affecting the process of ROS production, and monitor the cellular balance in oxidation-reduction. Providing effective prehospital care reducing oxidative stress will reduce cerebral damage and improve the status of health. Very few studies related to molecular mechanisms on the development of CVA were conducted in health care service field including nursing, therefore, it is important to encourage and continue related researches in the fields.

Understanding of Antioxidants Mechanism in Clinical Nursing Practice

Hyangkyu Lee,Younghwa Kim 한국생활환경학회 2013 한국생활환경학회지 Vol.20 No.6

The aim of this study is to provide fundamental knowledge for the efficient use of antioxidants in nursing intervention by identifying cellular damage by Reactive Oxygen Species (ROS), antioxidant defense mechanism, and a broad range of antioxidant use. This study investigated the known mechanisms and clinical applications of antioxidants through published literature reviews. The state of nursing research using antioxidants between the period 1966 and 2011 was reviewed using analysis criteria. The collection of literatures in this study was performed through a biomedical database, Pubmed, Korean research information service system, Riss4U, a science and technical database, ScienceDirect and Internet. Acute and chronic diseases are associated with the production of ROS in the human body, and the use of many antioxidants is being considered in order to reduce the pathological damage caused by ROS. Providing effective nursing intervention reducing oxidative stress will prevent various diseases and improve the status of health. To enhance the quality of clinical nursing practice, nurses need to understand the accurate mechanism of antioxidants, identify the risk factors affecting the process of ROS production, and monitor the cellular balance in oxidation-reduction.

Visceral adiposity and expression of clock genes in peripheral blood mononuclear cells: A pilot study

Kim, Sue,Lee, Hye-Sun,Park, Hyun-Ki,Linton, John A.,Lee, Ji-Won,Lee, Hyangkyu Informa UK (TaylorFrancis) 2017 Chronobiology international Vol.34 No.8

<P>Increasing evidence suggests a close interrelationship between disrupted circadian rhythms and obesity and metabolic disturbances. In particular, abdominal obesity, which contributes to the pathogenesis of metabolic disease, is associated with disrupted clock gene expression. However, little is known about the relationship between clock gene expression and accurate computed tomography (CT)-based measurements of visceral adiposity. Therefore, we examined the relationship between expression of clock genes in peripheral blood mononuclear cells (PBMCs) with visceral and subcutaneous adiposity in 75 healthy overweight or obese individuals. PBMCs were obtained from blood samples collected at 8 AM, and gene expression was analyzed by real-time reverse transcription polymerase chain reaction. Visceral and subcutaneous adiposity were measured by CT. Our results showed that visceral fat area was significantly positively correlated with BMAL1 and CRY1 mRNA levels and significantly negatively correlated with CLOCK, PER2, PER3 and CRY2 mRNA levels. In contrast, subcutaneous fat area was not correlated with the expression of any of the clock genes analyzed. After adjusting for multiple variables, visceral fat area was significantly associated with the expression of BMAL1, PER2 and CRY1. Taken together, our results indicate that visceral adiposity, but not subcutaneous adiposity, correlates with expression of clock genes in PBMCs.</P>

Caregiving Distress and Inflammatory Response among Family Caregivers of Cancer Patients

Yoonjoo Kim,Hyangkyu Lee,Heejung Kim,Sangyeon Suh,Hyun-Ki Park 한국간호과학회 2018 한국간호과학회 학술대회 Vol.2018 No.1

Purpose: Caregivers of cancer patients suffer from a wide range of problems, including fatigue, care burden, burnout and depression. Researches on cancer caregivers have demonstrated that the caregiving distress is related not only to mental but also to physical health such as dysregulation of inflammatory function. This study aimed to investigate the relationship between caregiving distress and serum IL-6 and TNF-α, which are implicated in immune function of caregivers of cancer patients. Methods: Total 63 family caregivers of cancer patients participated in this study. Caregiving distress (fatigue, caring burden, burnout, and depression) was measured by using structured self-report questionnaires. Two inflammatory cytokines, IL-6 and TNF-α levels were obtained using a high-sensitivity enzyme-linked immunosorbent assay (ELISA). Data were analyzed using descriptive statistics, t-test, ANOVA, Spearman correlation coefficient analysis, and Hierarchical multiple regression analyses. Results: There was a significant correlation between fatigue, caring burden, burnout, depression. Caregiving distress was associated with the increased serum IL-6 and TNF-α levels. Gender, experience in radiotherapy, length of caregiving, and fatigue are associating factors for the levels of cytokines. Conclusions: Caregiving distress is a negative experience that affects mental health and inflammatory response to family caregivers. It is necessary to develop practical nursing intervention that supports caregivers and manages caregiving distress.

Risk factors for postoperative delirium in the elderly who underwent spine surgery postoperative cognitive decline and identifying associated biomarkers using exosomes

Wonhee Baek,Hyangkyu Lee 한국간호과학회 2021 한국간호과학회 학술대회 Vol.2021 No.10

Background: Since there is a high incidence of delirium after spinal surgery and the prognosis is poor, it is important to predict and detect delirium early by identifying risk factors for delirium before surgery. In addition, since delirium symptoms are episodic and are common in the elderly, the rate of diagnosis by the medical staff is low. Therefore, it is necessary to search for biomarkers that are helpful in the prediction and diagnosis of delirium. Based on Jeffrey and Stacie"s (2013) model of the potential relationships among anesthesia, surgery, and the ultimate development of long-term cognitive impairment, this study aimed to investigate the risk factors affecting postoperative delirium in older adults who have undergone spine surgery, identify the association between postoperative delirium and cognitive decline, and discover the biological markers. Methods: Data collection was carried out from October 23, 2019 to May 20, 2020 on 100 patients over 70 years of age who underwent spinal surgery at S city Y hospital. Urine exosomes were analyzed for tau, UCH-L1, NFL, and GFAP using a single molecule array (Simoa). The collected data were analyzed using the R program (Version 4.0.0). Results: There were differences between delirious and non-delirious participants with regard to their past history of psychiatric illness and use of benzodiazepines (p=.005 and p=.026, respectively). There was a difference in the ratios of preoperative WBC and total protein between delirious and non-delirious participants (p=.013, p=.013, respectively). There was a difference in the ratios of CRP and eosinophil count on the day of operation between delirious and non-delirious participants (p=.05 and p=.013, respectively). Tau and UCH-L1 concentrations were found to be high in the severely delirious participants (p=.002 and p=.001, respectively). The MMSE scores upon discharge (p<.012) and the differences between the preoperative and postoperative MMSE scores (p<.009) were significantly different between delirious and non-delirious participants. Conclusion: Based on these results, medical staff will be able to accurately investigate risk factors before surgery, classify high-risk patients, and predict and detect delirium early. In addition, since delirious participants are at risk for cognitive decline, follow-up and preventive interventions for cognitive dysfunction will be required, depending on the patient. In addition, exosome analysis of the urine of patients showed that there was a high degree of brain injury in delirious patients after surgery.

Assessment of Risk Factors for Postoperative Delirium in Older Adults Who Underwent Spinal Surgery and Identifying Associated Biomarkers Using Exosomal Protein

Baek, Wonhee,Lee, JuHee,Jang, Yeonsoo,Kim, Jeongmin,Shin, Dong Ah,Park, Hyunki,Koo, Bon-Nyeo,Lee, Hyangkyu 한국간호과학회 2023 Journal of Korean Academy of Nursing Vol.53 No.4

Purpose: With an increase in the aging population, the number of patients with degenerative spinal diseases undergoing surgery has risen, as has the incidence of postoperative delirium. This study aimed to investigate the risk factors affecting postoperative delirium in older adults who had undergone spine surgery and to identify the associated biomarkers. Methods: This study is a prospective study. Data of 100 patients aged ≥ 70 years who underwent spinal surgery were analyzed. Demographic data, medical history, clinical characteristics, cognitive function, depression symptoms, functional status, frailty, and nutritional status were investigated to identify the risk factors for delirium. The Confusion Assessment Method, Delirium Rating Scale-R-98, and Nursing Delirium Scale were also used for diagnosing delirium. To discover the biomarkers, urine extracellular vesicles (EVs) were analyzed for tau, ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), neurofilament light, and glial fibrillary acidic protein using digital immunoassay technology. Results: Nine patients were excluded, and data obtained from the remaining 91 were analyzed. Among them, 18 (19.8%) developed delirium. Differences were observed between participants with and without delirium in the contexts of a history of mental disorder and use of benzodiazepines (p = .005 and p = .026, respectively). Tau and UCH-L1—concentrations of urine EVs—were comparatively higher in participants with severe delirium than that in participants without delirium (p = .002 and p = .001, respectively). Conclusion: These findings can assist clinicians in accurately identifying the risk factors before surgery, classifying high-risk patients, and predicting and detecting delirium in older patients. Moreover, urine EV analysis revealed that postoperative delirium following spinal surgery is most likely associated with brain damage.

Nurses confidence in performance, care burden, and educational needs for ostomy care

Geonah Kim,Hyangkyu Lee,Yunjin Lee,Imkyung Kim 한국간호과학회 2023 한국간호과학회 학술대회 Vol.2023 No.11

Impact of Fatigue on Quality of Life in Patients with Thyroid Cancer after Total Thyroidectomy

Jungeun Kim,Hyangkyu Lee,Sue Kim,Woonyoun Chung 한국간호과학회 2015 한국간호과학회 학술대회 Vol.2015 No.10

Utilizing a Digital Health Intervention to Enhance Physical, Psychosocial, and Cognitive Health in Older Adults: Subgroup Analysis Based on Engagement Level

Jeongeun Choi,Hyeonmi Cho,Hyangkyu Lee,Yu-Jin Kwon 한국간호과학회 2023 한국간호과학회 학술대회 Vol.2023 No.11

Exosomal Protein Profiles as Novel Biomarkers in Weight Gain After Kidney Transplantation: A Pilot Study

Cho Young-Eun,Lee Hyangkyu,Kim Hyungsuk,Yun Sijung,Cashion Ann 한국기초간호학회 2020 Journal of korean biological nursing science Vol.22 No.2

Purpose: Weight gain after kidney transplantation is a critical factor that can lead to poor outcomes with cardiovascular complications. Many studies have been conducted to identify predictive markers of future weight changes at the time of transplant. Recently, circulating exosomes and its contents including miRNAs and proteins have attracted attention as potential biomarkers. In this pilot study, we investigated exosomal proteins and weight change after kidney transplant. Methods: Recipients (n=10) were classified into two groups; weight gainers (n=5, 9.7±4.4kg) and weight losers (n=5, -6.4±1.8kg) based on their weight changes at 12-months posttransplant. Based on the exosomal protein profiles obtained by the LC-MS/MS, differentially expressed proteins were identified between the groups. Results: Concentration and the mean size of exosomes significantly increased at 12-months compared to the baseline (p=.009) in the total group. Eleven exosomal proteins were found at the baseline as differentially expressed between the two groups. In the weight gain group, complement proteins including HV169, C3, C4B, and C4A, were significantly upregulated. Conclusion: Our pilot study suggests that exosomal complementary proteins are associated with weight gain after kidney transplantation. Further studies are needed to clarify the role of these exosomal proteins in the underlying mechanisms of weight changes in kidney transplant recipients.