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Huiping Yu,Zhihao Tong,Pei Chen,Anwen Cai,Fei Qin 한국물리학회 2020 Current Applied Physics Vol.20 No.4
This paper is devoted to studying the thermal and mechanical properties of aminated graphene (AG)/epoxy nanocomposites connected by covalent bond using molecular dynamics (MD) simulation. The effects of crosslinking degree, mass fraction and functionalized graphene (FG) type on AG/epoxy nanocomposites are considered. The elasticity modulus (E), the glass transition temperature (Tg), the coefficient of thermal expansion (CTE) and the interfacial energy (Eint) are also investigated. The MD simulation results indicate that, when the mass fraction of AG is between 1.2% and 3.1% and crosslinking degree reaches about 70%, the E, Tg, Eint and CTE of AG/epoxy nanocomposites are significantly improved compared with those of pure epoxy and graphene/ epoxy nanocomposites. The reason is that AG not only possesses some excellent thermodynamic properties of graphene, but also has the function of curing agent to crosslink with epoxy monomer to form the carbon-nitrogen (C–N) covalent bond. A better interfacial interaction between nanoparticles and epoxy is essential in enhancing the thermal and mechanical properties of nanocomposite materials, which will provide a microscopic theoretical basis for the study of epoxy nanocomposites.
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Zhimin Yu,Mou-Ming Zhao,Huiping Li,Hai-Feng Zhao,Qingli Zhang,Chunyan Wan,Huipin Li 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.4
High gravity (HG) or very high gravity (VHG)brewing has become popular in modern breweries due to its economic and product quality advantages. However,there are the negative impacts such as the fermentation performance of brewer’s yeast in HG or VHG wort, which are closely related to changes in cell physiological activity. In the present study, 3 kinds of worts, with different gravities, were used to examine the systematic effects on fermentation performance and physiological activity of lager yeast FBY009505 (Saccharomyces pastorianus) and ale yeast FBY0099 (Saccharomyces cerevisiae), as well as the resulting beer flavor. Results showed that the responses of FBY009505 and FBY0099 to the HG or VHG worts were similar. The specific fermentation rate and viability of cropped yeast of FBY009505 and FBY0099 were decreased with increasing wort gravity. The increased wort gravity resulted in the increase of energy charge and the decrease of α-glucosides transport rate and glycolytic enzyme activities. Moreover, the environmental stresses in the HG or VHG wort showed a higher inhibitory activity against α-glucoside transport than glycolytic enzymes. The content of intracellular trehalose and glycerol of FBY009505 and FBY0099 increased with the increase in wort gravity. The results from this study provided a potential means to systematically understand the physiology of brewer’s yeast under HG or VHG conditions. High gravity (HG) or very high gravity (VHG)brewing has become popular in modern breweries due to its economic and product quality advantages. However,there are the negative impacts such as the fermentation performance of brewer’s yeast in HG or VHG wort, which are closely related to changes in cell physiological activity. In the present study, 3 kinds of worts, with different gravities, were used to examine the systematic effects on fermentation performance and physiological activity of lager yeast FBY009505 (Saccharomyces pastorianus) and ale yeast FBY0099 (Saccharomyces cerevisiae), as well as the resulting beer flavor. Results showed that the responses of FBY009505 and FBY0099 to the HG or VHG worts were similar. The specific fermentation rate and viability of cropped yeast of FBY009505 and FBY0099 were decreased with increasing wort gravity. The increased wort gravity resulted in the increase of energy charge and the decrease of α-glucosides transport rate and glycolytic enzyme activities. Moreover, the environmental stresses in the HG or VHG wort showed a higher inhibitory activity against α-glucoside transport than glycolytic enzymes. The content of intracellular trehalose and glycerol of FBY009505 and FBY0099 increased with the increase in wort gravity. The results from this study provided a potential means to systematically understand the physiology of brewer’s yeast under HG or VHG conditions.
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Naxin Sun,Huiping Liu,Xiaowei Zhang,Hongni Wang,Shaojuan Liu,Pei Chen,Weijie Yu,Kai Liu 한국축산식품학회 2018 한국축산식품학회지 Vol.38 No.2
To shorten the production cycle of Zaodan, this study first pickled Zaodan by a novel technology -vacuum decompression technology. Vacuum decompression technology could reduce the pickling time of Zaodan from 20wkto about 9wk.The protein content, moisture and pH of the Zaodan egg white gradually decreased with a concomitant increase in salt during the pickling process. The total sulfhydryl group (SH) group content of the egg white proteins was increased to 2.43×10-3mol/L after being pickled for 30 d, whereas the content of disulphide bonds (SS) was reduced to 23.35×10-3mol/L. The surface hydrophobicity was lowest after pickling for 30 d. In addition, great changes occurred in the secondary structure of the egg white proteins after pickling for 20 d. The disappearance of ovomucin was noticeable based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis.
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Peirong Li,Tongbing Su,Shuancang Yu,Huiping Wang,Weihong Wan,Yangjun Yu,Deshuang Zhang,Xiuyun Zhao,Changlong Wen,Fenglan Zhang 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.3
Rapid, economical, and reliable genotyping is an important requirement for germplasm analysis and cultivar identifi cationin crop species. Chinese cabbage ( Brassica rapa L. subsp. pekinensis (Lour.) Hanelt) originated in China and is now aneconomically important vegetable crop worldwide, especially in East Asia. In this study, we evaluated 1167 single nucleotidepolymorphisms (SNPs) among 166 representative Chinese cabbage inbred lines using a KASP genotyping assay. Onthe basis of polymorphisms and principal component analysis, we selected 60 core SNPs distributed on all Brassica rapachromosomes with allele frequencies suffi ciently balanced so as to provide adequate information for genetic identifi cation. The core set of SNPs was used for construction of a neighbor-joining dendrogram, in which the 166 inbred lines wereclustered into spring, summer, and autumn ecotype groups. Clustering of the ecotype groups was better resolved than thatachieved with 1167 and 360 polymorphic SNP datasets. Stability and resolution of the core SNP markers were tested using178 commercial hybrid Chinese cabbage cultivars to confi rm their utility in genetic identifi cation. The set of 60 informativeand stable SNP markers showed high discriminatory power and relatively uniform genomic distribution (4–9 markers perchromosome). The SNPs represent a cost-effi cient and accurate marker set for germplasm analysis and cultivar identifi cationand are suitable for molecular marker-assisted breeding in Chinese cabbage.
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