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Hu-Quan Yin,Youn-Su Kim,You-Jin Choi,김윤철,손동환,Shi-Yong Ryu,이병훈 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.5
Tanshinone IIA is one of the most abundant constituents of the root of Salvia miltiorrhiza BUNGE which exerts antioxidant and anti-inflammatory actions in many experimental disease models. In the present study, we demonstrated that the standardized fraction of S. miltiorrhiza (Sm-SF) was able to protect RAW 264.7 cells from ethanol- and lipopolysaccharide (LPS)- induced production of superoxide radical, activation of NADPH oxidase and subsequently death of the cells. Among four main components of Sm-SF, tanshinone IIA was the most potent in protecting cells from LPS-and ethanol-induced cytotoxicity. LPS or ethanol induced the expression of CD14, iNOS, and SCD1 and decreased RXR-α, which was completely reversed by tanshinone IIA. In H4IIEC3 cells, 10 μM tanshinone IIA effectively blocked ethanolinduced fat accumulation as evidenced by Nile Red binding assay. These results indicate that tanshinone IIA may have potential to inhibit alcoholic liver disease by reducing LPS- and ethanol- induced Kupffer cell sensitization, inhibiting synthesis of reactive oxygen/nitrogen species, inhibiting fatty acid synthesis and stimulating fatty acid oxidation.
Bioconversion of Ginsenoside Rd into Compound K by Lactobacillus pentosus DC101 Isolated from Kimchi
Quan, Lin-Hu,Cheng, Le-Qin,Kim, Ho-Bin,Kim, Ju-Han,Son, Na-Ri,Kim, Se-Young,Jin, Hyun-O,Yang, Deok-Chun The Korean Society of Ginseng 2010 Journal of Ginseng Research Vol.34 No.4
Ginsenosides are the principal components responsible for the pharmacological and biological activities of ginseng. Ginsenoside Rd was transformed into compound K using cell-free extracts of food microorganisms, with Lactobacillus pentosus DC101 isolated from kimchi (traditional Korean fermented food) used for this conversion. The optimum time for the conversion was about 72 h at a constant pH of 7.0 and an optimum temperature of about $30^{\circ}C$. The transformation products were identified by thin-layer chromatography and high-performance liquid chromatography, and their structures were assigned using nuclear magnetic resonance analysis. Generally, ginsenoside Rd was converted into ginsenoside F2 by 36 h post-reaction. Consequently, over 97% of ginsenoside Rd was decomposed and converted into compound K by 72 h post-reaction. The bioconversion pathway to produce compound K is as follows: ginsenoside Rd$\rightarrow$ginsenoside F2$\rightarrow$compound K.
Temporal Changes in the Hepatic Fatty Liver in Mice Receiving Standard Lieber-DeCarli Diet
Hu-Quan Yin,Byung-Hoon Lee 한국독성학회 2008 Toxicological Research Vol.24 No.2
Chronic exposure to ethanol induces cumulative damage to the liver starting from fatty infiltration to cirrhosis depending on the dose and duration of exposure. The whole process leading to the development of alcoholic liver disease is very complex and the mechanisms involved are not fully understood. Among many experimental animal models, Lieber-DeCarli liquid diet provides moderate to severe pathophysiological outcome depending on the compositional changes. In the present study, we investigated the temporal changes in the early phase hepatic disease in rats fed with standard Lieber-DeCarli diet. Male Wistar rats were fed with Lieber-Decarli ethanol diet for 6 weeks and the liver samples were obtained after 2, 4 and 6 weeks. Mild fatty infiltration was observed in 2 weeks of feeding and it became evident in 4 and 6 week samples. The level of hepatic triglyceride showed a good agreement with the data obtained in the pathological analysis. Feeding mice with ethanol diet resulted in the maturation and translocation of SREBP-1 to nucleus in the liver. Western blot analysis of the pooled liver sample of control and ethanol fed animals showed a clear-cut time-dependent increase in the expression of nSREBP-1. These data provide important information for selecting proper time point in experimental intervention study in the field of drug development for alcoholic liver disease.
Quan, Lin-Hu,Min, Jin-Woo,Yang, Dong-Uk,Kim, Yeon-Ju,Yang, Deok-Chun Springer International 2012 Applied microbiology and biotechnology Vol.94 No.2
<P>Microbacterium esteraromaticum was isolated from ginseng field. The beta-glucosidase gene (bgp1) from M. esteraromaticum was cloned and expressed in Escherichia coli BL21 (DE3). The bgp1 gene consists of 2,496 bp encoding 831 amino acids which have homology to the glycosyl hydrolase family 3 protein domain. The recombinant beta-glucosidase enzyme (Bgp1) was purified and characterized. The molecular mass of purified Bgp1 was 87.5 kDa, as determined by SDS-PAGE. Using 0.1 mg ml(-1) enzyme in 20 mM sodium phosphate buffer at 37A degrees C and pH 7.0, 1.0 mg ml(-1) ginsenoside Rb1 was transformed into 0.444 mg ml(-1) ginsenoside Rg3 within 6 h. The Bgp1 sequentially hydrolyzed the outer and inner glucose attached to the C-20 position of ginsenosides Rb1. Bgp1 hydrolyzed the ginsenoside Rb1 along the following pathway: Rb1 -> aEuro parts per thousand Rd -> aEuro parts per thousand 20(S)-Rg3. This is the first report of the biotransformation of ginsenoside Rb1 to ginsenoside 20(S)-Rg3 using the recombinant beta-glucosidase.</P>
Quan Zhou,Song Yang,Heng Zhang,Fei Chang,Hu Li,Hu Pan,Wei Xue,De-Yu Hu 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.31 No.-
A comparative study on the activity of La2O3 and two kinds of nano La2O3 catalysts prepared usingsonochemical (nano La2O3-S) and hydrothermal methods in the transesterification to produce biodieselwas conducted. The relatively high activity of nano La2O3 catalysts may be ascribed to their high basestrength, large base amount, small particle size and large BET surface areas. Nano La2O3-S was selectedfor further optimisation due to its simple preparation procedure and short preparation time. The FAMEcontent and yield obtained were successively 97.6% and 90.3% under optimal conditions. Moreover, nanoLa2O3-S showed a remarkable tolerance to FFA.
Quanli Hu,Myung-Soo Kim 한국탄소학회 2008 Carbon Letters Vol.9 No.4
Carbon blacks could be used as the filler for the electromagnetic interference (EMI) shielding. The poly vinyl alcohol (PVA) and polyvinylidene fluoride (PVDF) were used as the matrix for the carbon black fillers. Porous carbon blacks were prepared by CO2 activation. The activation was performed by treating the carbon blacks in CO2 to different degrees of burnoff. During the activation, the enlargement of pore diameters, and development of microporous and mesoporous structures were introduced in the carbon blacks, resulting in an increase of extremely large specific surface areas. The porosity of carbon blacks was an increasing function of the degree of burn-off. The surface area increased from 80 m2/g to 1142 m2/g and the total pore volume increased from 0.14073 cc·g-1 to 0.9343 cc·g-1. Also, the C=O functional group characterized by aldehydes, ketones, carboxylic acids and esters was enhanced during the activation process. The EMI shielding effectiveness (SE) of raw N330 carbon blacks filled with PVA was about 1 dB and those of the activated carbon blacks increased to the values between 6 and 9 dB. The EMI SE of raw N330 carbon blacks filled with PVDF was about 7 dB and the EMI SE increased to the range from 11 to 15 dB by the activation.
Quan, Lin-Hu,Piao, Jin-Ying,Min, Jin-Woo,Yang, Dong-Uk,Lee, Hee Nyeong,Yang, Deok Chun Sociedade Brasileira de Microbiologia 2011 Brazilian journal of microbiology Vol.42 No.3
<P>About 40 different types of ginsenoside (ginseng saponin), a major pharmacological component of ginseng, have been identified along with their physiological activities. Among these, compound K has been reported to prevent the development of and the metastasis of cancer by blocking the formation of tumors and suppressing the invasion of cancerous cells. In this study, ginsenoside Rb1 was converted into compound K via interaction with the enzyme secreted by β-glucosidase active bacteria, <I>Leuconostoc citreum</I> LH1, extracted from kimchi. The optimum time for the conversion of Rb1 to compound K was about 72 hrs at a constant pH of 6.0 and an optimum temperature of about 30°C. Under optimal conditions, ginsenoside Rb1 was decomposed and converted into compound K by 72 hrs post-reaction (99%). Both TLC and HPLC were used to analyze the enzymatic reaction. Ginsenoside Rb1 was consecutively converted to ginsenoside Rd, F2, and compound K via the hydrolyses of 20-C β-(1 → 6)-glucoside, 3-C β-(1 → 2)-glucoside, and 3-C β-glucose of ginsenoside Rb1.</P>