Impact of Interferon-Based Treatment on Quality of Life and Work Related Productivity from the Korean Cohort in the MOSAIC Study

( Sang Hoon Ahn ),( Won Hyeok Choe ),( Yoon Jun Kim ),( Jeong Heo ),( Dorota Latarska-smuga ),( Jiho Kang ),( Seung Woon Paik ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Chronic Hepatitis C Virus (HCV) infection increases the risk for progressive liver disease, hepatocellular carcinoma and negatively impacts the patient’s quality of life. HCV treatment is evolving with direct acting antivirals but IFN based therapy has been the standard of care for many years and remains available in some countries. The MOSAIC study aims to characterize patients with chronic HCV infection and assess the impact of IFN-containing treatment on health-related quality of life, work related productivity and health care utilization. Methods: MOSAIC is an international prospective multicenter observational study that has been conducted in 20 countries. Consecutive patients with chronic HCV infection were enrolled and those who initiated an IFN based regimen were prospectively followed for 48 weeks. We report results from the Korean cohort Results: 100 patients were enrolled: 86 were treatment naïve and 14 were treatment experienced. 33 patients initiated an IFN based regimen: 6 patients started IFN + RBV, 26 patients started Peg-IFN + RBV, none started Peg-IFN + RBV + DAA and 1 patient received other treatment. Among the treated cohort, demographic and disease characteristics were the following: the mean age was 54.5 years; 14 patients were male. 14 had minimal or no fibrosis, 2 portal fibrosis, 3 bridging fibrosis and 6 patients suffered from cirrhosis. HCV Genotype distribution was as follows: genotype 1: 11; genotype 2: 19 and genotype 3: 3. Table 1 describes the results at baseline and changes over 4, 12 and 48 weeks and end-of-treatment (EOT) for the quality of life and work productivity outcome measures (EQ-5D-5L, HCV-PRO and WPAI). Conclusions: Results from the Korean cohort of the MOSAIC study show a moderate trend for deterioration of health-related quality of life and work productivity associated with IFN based treatment for patients with chronic HCV infection during treatment period. Acknowledgements: The design, study conduct, analysis, and financial support of MOSAIC study were provided by AbbVie. AbbVie participated in the interpretation of data, review, and approval of the content of the abstract. All authors had access to all relevant data and participated in writing, review, and approval of this abstract. Medical writing support was provided by Olivier Van de Steen of Medeor-consulting, funded by AbbVie. Disclosures: Sang Hoon Ahn: served as an advisor and lecturer for Bristol-Myers Squibb, Gilead Sciences, F.Hoffmann-La Roche, Merck, AbbVie, and has received unrestricted grants from Bristol-Myers Squibb, Gilead Sciences, and F. Hoffmann-La Roche for investigator- initiated trials Won Hyeok Choe: Nothing to disclosure Yoon Jun Kim: Nothing to disclosure Jeong Heo: received a grant from GSK; Research support from BMS, and Roche; Advisor for Abbvie, BMS, Gilead Sciences, Pharma Essentia, SillaJen, and Johnson & Johnson. Dorota Latarska-Smuga, Jiho Kang: are employees of AbbVie, Inc. and may hold stock or stock options. Seung Woon Paik: received grant and research support from AbbVie, BMS, Gilead, GSK, Merck, Novartis, and Roche

동종 조혈모세포이식 후 발생한 치명적 홍역 폐렴 1예

백창렬,이동건,최정현,정현화,조유경,박훈준,이승훈,박윤희,이교영,민우성,김춘추,신완식 대한감염학회 2001 감염 Vol.33 No.4

As a result of the enlarging pool of unvaccinated children and young adults, there has been an increase in measles in our countries. In these situation, it has been reported that measles associated pneumoinia is easily complicated with fatal respiratory failure, espycially in immunocompromised patients. Herein we report the case of lethal measles pneumonia after allogenic hematopoietic stem cell transplantation in adults proven by autopsy. Recently, one case of measles was encountered in 39-year-old female patients after allogenic bone marrow transplanted case (chronic myelogenous leukemia), who progressed into interstitial pneumonia pattern, despite treatment including antibiotics, immunoglobulin. The patient died of giant cell pneumonia compatible with that of measles which was comfirmed in the section of necropsy lung specimen. (Korean J Infect Dis 33:301∼309, 2001)

Daunomycin이 Mouse 간장의 Alkaline Phosphatase 활성에 미치는 영향

백두진,김훈기,정호삼 한양대학교 의과대학 1987 한양의대 학술지 Vol.7 No.2

Daunomycin is a member of the anthracycline class of antitumor antibiotics. And it was isolated from cultures of Streptomyces peucetius in 1963. Cemically, it was found to consist of a pigmented aglycone (daunomycinone) bound to an aminosugar, daunosamine. The biochemical mode of cytotoxic action of daunomycin results from its binding to DNA possibly by intercalation between the base pairs, thereby inhibiting the DNA polymerases. The author has investigated the effect of daunomycin on the mouse liver histochemically observing the change in the activity of alkaline phosphatase. The animals treated with 16 mg per kg of daunomycin wee sacrified at 6, 12, 24 and 36 hours after drug administration. The animals of control group were administered only water for injection. The liver specimens were fixed in 10% neutral formalin at 4℃ and sectioned at 16? In thickness in a frozen cryostat. The activity of alkaline phosphatase was evaluated histochemically by Gomori's method. The results were as follows. 1. The activity of alkaline phosphatase was moderate positive at the periportal and intermediate zones and trace positive at the centrilobular zone of the hepatic lobule in 6 hours daunomycin treated group. Ad the time goes by, the activity of alkaline phosphatase was decreased in the hepatic lobule. At 24 hours after administraton of daunomycin, negative reaction at the centrilobular zone, trace positive at the intermediate zone and weak positive at the periportal zone of the hepatic lobule were seen. Consequently, it is suggested that daunomycin decreases the activity of alkaline phoshatase in the liver, due probably to its cytotoxic effect on the hepatocyte.

스포츠생리학 : Creatine 투여가 쇼트트랙 스피드 스케이팅 선수의 운동 수행력과 혈중 피로 요인에 미치는 영향

백일영(Paik11-Young),우진희(WooJin-Hee),채지훈(ChaefJi-Hoon) 한국체육학회 2000 한국체육학회지 Vol.39 No.2

본 연구의 목적은 주니어 국가대표급 쇼트트랙 스피드 스케이팅 선수 5명을 대상으로 Creatine(Cr)을 구강 투여하여, 투여된 Cr이 최대 운동 부하 수행력에 미치는 영향과 혈중 젖산, 무기인산, 암모니아, pH등과 같은 생리학적 피로 요소의 변화를 고찰하여, Cr 투여가 운동수행에 미치는 영향뿐만 아니라, 피로 요소들의 변화에 미치는 영향을 연구하는데 있으며, 특히, 중추신경계의 피로물질로 알려진 serotonin(5-HT)의 변화를 고찰함으로써 Cr의 피로 유발 지연 효과를 규명하는데 그 목적이 있다. 운동 부하 테스트는 정규 국제규격인 쇼트트랙 전용 경기장에서 Cr을 투여하기 전 500m와 3,000m를 충분한 휴식을 두고 자신의 최대역량으로 주파하게 하는 형태를 취하였으며, Cr의 투여는 하루에 20g씩 5일간 투여하였고,6일째 되는 날 같은 조건으로 재측정 하였다. 운동 수행력의 변화는 각각의 실험 조건에서 Cr을 투여한 실험 조건과 투여하지 않은 실험 조건을 비교해서 유의한 향상이 나타나지 않았으나(p>.05), 쇼트트랙 경기의 특성을 고려할 때, Cr의 투여로 순위를 결정지을 수 있는 기록이 단축 되는 것으로 나타났다. 혈중 젖산의 변화는 운동 종료시 Cr을 투여한 실험조건에서 투여하지 않은 실험조건에서 보다 더 낮은 혈중 젖산 농도를 보였으며, 통계적으로도 유의한 차이가 나타났다(p<.05). 혈중 암모니아와 무기인산의 변화 역시, 혈중 젖산 농도 변화와 마찬가지로 운동시 모든 경우에 증가하는 현상을 보였으며, 운동 종료시 Cr을 투여한 실험조건에서 투여하지 않은 실험조건에서 보다 낮은 혈중 젖산 농도를 보였고 통계적으로 유의한 차이가 나타났다(p<.05). 혈중 pH의 변화는 운동 종료시 Cr을 투여한 실험조건에서 투여하지 않은 실험조건에서 보다 높은 혈중 pH를 보였으나, 통계적으로 유의한 차이가 나타나지 않았다(p>.05). 혈중 5-HT의 농도 변화는 두 그룹 모두에서 Cr을 투여한 실험 조건에서 더 높은 5-HT의 농도를 보였고, 통계적으로도 유의한 차이가 나타났다(p<.05). 전체적으로 운동이 반복되면서 5-HT의 농도는 일정한 형태로 증가하다가 회복시에 감소하는 양상을 보였지만, 피로유발 요인이라고 단정하기엔 무리가 있다고 본다. 따라서, 쇼트트랙과 같은 단시간의 고강도 운동 형태의 경기 종목 선수에게 있어서, Cr의 효율적인 공급은 운동 수행시 에너지 보충제로서의 역할을 충분히 수행할 수 있는 잠재적 역량을 가지고 있을 뿐만 아니라, 피로 유발 물질의 감소와 그로 인한 운동 수행력의 향상에 많은 도움을 줄 수 있을 것으로 본다. 또한, 대사적 생성기전과 피로인식 기전의 개념으로 5-HT에 대한 연구가 추가적으로 수행되어, 피로유발 요인으로서 5-HT에 대한 과학적인 증거의 제시가 이루어져야 할 것으로 본다. The purpose of this study was to examine the effect of oral creatine supplementation on exercise performance, and to follow the changes of blood fatigue elements, such as lactate, ammonia, inorganic phosphate, pH & 5-HT. All of the tests were performed on a international standard arena ice rink. The subject of present study was divided into two groups; 500m skaters(500-AT), and 3,000m skaters (3,000-AT). Each skaters participated in their own skating distances(500 and 3,000m). After the preliminary test, all subjects were given a 5-day supplementation of Creatine monohydrate at a rate of 20g·d^-1.When the study was performed, we could not find a significant difference in developing a record by Cr supplementation(p>.05). However, as the rank of skating is determined by hundreds of second, we could find a result, which can change the rank of the skating competition by the Cr supplementation. There were significant differences in mean blood lactate between Cr supplementation and Cr non-supplementation for the 500-AT and the 3,000-AT groups(p< .05). Also, there were big significant differences in mean blood inorganic phosphate and ammonia concentrations between Cr supplementation and Cr non-supplementation for the 500-AT and the 3,000-AT groups(p < .05). The mean blood pH in Cr supplementation experimental condition was higher than Cr non-supplementation experimental condition, but we could not find a significant differences in mean blood pH between Cr supplementation and Cr non-supplementation for the 500-AT and the 3,000-AT groups(p >.05).Blood 5-HT concentrations in Cr supplementation experimental condition was higher than non supplementation experimental condition, and there were significant differences between Cr supplementation and Cr non-supplementation for the 500-AT and the 3,000-AT groups.(p<.05). However, it was difficult to explain that 5-HT occurs fatigue unless the physiological mechanism of 5-HT was provided.Therefore, this study concluded that Cr supplementation had a ergogenic effect on energy metabolism. Especially, in a high intensive exercise as short-track skating, Cr supplementation will help for fatigue elements reduction and exercise performance improvement. Also, we believe the additional research of 5-HT must be accomplished so that we can provide the scientific evidence of fatigue, which was cause by 5-HT.

Recognition of Pet Heart Failure Using Dual-stream Convolutional Neural Network

lae-Hyun Paik十,Jun-Young Oh+, Euijong Lee, and Ji-Hoon Jeong 충북대학교 컴퓨터 정보통신 연구소 2022 컴퓨터정보통신연구 Vol.30 No.2

본문 참고

실리콘 겔에 활성화된 복강 대식세포의 interleukin-6 및 tumor necrosis factor-α에 의한 섬유모세포 중식 자극

김환묵,한상배,이백권,이종원,한기택,천지훈 大韓成形外科學會 1998 Archives of Plastic Surgery Vol.25 No.5

Silicone gel breast implants may induce local(fibrous capsular contracture) or systemic(rheumatoid arthritis, systemic sclerosis, etc) complications. The exact mechanism of fibrous capsular contracture has not been fully understood. In the present study, we tried to find out the effect of silicone gel on the fibroblast proliferation which has been known as a major contributing factor in fibrous capsular contracture formation. In vitro, activated macrophages are known to secrete monokines which affect fibroblast proliferation and collagen synthesis. And tumour necrosis factor-α(TNF-α) and interleukin-6(IL-6), which were released by macrophages, were reported as potent stimulator of fibroblast proliferation. The goal of this study is to investigate the role of macrophages and tumour necrosis factor-αor interleukin-6 in the interaction of fibroblasts and silicone gel. We designed four groups, two experimental and two control, using Institute for Cancer Research(ICR) mouse peritioneal macrophage and silicone gel. For the preparation of the conditioned medium of macrophages, peritoneal macrophages were prepared and cultured for 24 hours on the silicone gel-coated and naked (not coated) surface [silicone gel-macrophage conditioned medium(SCM; experimental group) and normal polystyrene-macrophage conditioned medium(NCM; control group) respectively]. To correct the effect of 10% fetal bovine serum which was included in Rapid Prototyping and Manufacturing Institute (RPMI) 1640 medium and draw the effect only by macrophages, the RPMI 1640 medium with 10% fetal bovine serum was cultured by the same method on the silicone gel-coated and naked surface (silicone gel-macrophage free conditioned medium; SFM and normal polystyrene-macrophage free conditioned medium; NFM respectively). Each conditioned medium was added onto NIH 3T3 fibroblasts culture at a final 25% concentration of total culture medium and followed by the cultivation for 24 hours. For antibody neutralizing experiments, each conditioned medium was preincubated with polyclonal rabbit anti-mouse TNF-α antibody or polyclonal rat anti-mouse IL-6 antibody for 1 hour and then, conditioned medium with antibody was added to the culture medium of NIH 3T3 fibroblasts by the same method. After 24 hours cultivation, total number of viable fibroblast(cell growth), DNA synthesis and collagen synthesis of fibroblasts with each medium were measured by sulforhodamine B(SRB) assay, 3H-thymidine and 3H-proline incorporation respectively. The results were as follows: 1. In the experiment about the effect of the conditioned medium on the fibroblast activity, the experimental group(SCM), compared with the control group(NCM), showed a significant increase of the cell growth (p<0.01), a significant decrease of DNA synthesis(p<0.001), but no significant difference in the collagen synthesis. 2. In the experiment about the effect of polyclonal rabbit anti-mouse TNF-α antibody on the fibroblast activity, after the addition of antibody the experimental group, compared with the control group, showed a significant decrease of the cell growth(p<0.001), a significant increase of DNA synthesis(p<0.01), but no significant difference in the collagen syn thesis. 3. In the experiment about the effect of polyclonal rat anti-mouse IL-6 antibody on the fibroblast activity, after the addition of antibody the experimental group, compared with the control group, showed a significant decrease of the cell growth(p<0.001), a significant increase of DNA synthesis(p<0.0001), but no significant difference in the collagen synthesis. In conclusion, culture supernatants (conditioned medium) of peritoneal macrophages, activated by silicone gel, stimulate the NIH 3T3 fibroblast proliferation. TNF-α and IL-6, products of macrophage, are involved in the stimulation of NIH 3T3 fibroblast proliferation in an in vitro condition.

고교 축구선수의 경기 후 회복방법의 차이가 혈중 염증, 근 손상 및 피로 지표에 미치는 영향

백승훈(Seung Hoon Paik),백일영(Il Young Paik),서상훈(Sang Hoon Suh),조수연(Su Youn Cho),노희태(Hee Tae Roh) 한국사회체육학회 2014 한국사회체육학회지 Vol.0 No.56

The purpose of the current study was to investigate the effects of different recovery treatments on serum inflammation, muscle damage and fatigue variables after a game of soccer players in high school. The subjects of current study were 10 soccer players in high school without any medical complications. All subjects received both recovery treatments (contrast bath and massage) for once and a control application; therefore, all underwent 3 experimental conditions. Blood sampling taken at rest, at immediately after the match, and at 60 minutes recovery. From the three blood samples, the parameters of inflammation and fatigue were analyzed. Additional blood sampling was taken at 24 hours recovery for the analysis of the long term effect of the applications on the parameters of muscle damage. The concentration of interleukin-6(IL-6), creatine kinase(CK), lactate dehydrogenase (LDH), and lactate were shown to significantly increase at immediately after the match in all experimental conditions(p<.05). For inflammation responses the concentration of IL-6 60min recovery appeared to be significantly lower in contrast bath and massage treatments compared to control treatment(p<.05). Also, for muscle damages the concentration of CK 24h recovery appeared to be significantly lower in massage treatment compared to contrast bath and control treatments(p<.05). On the base of the results of the current study, it is suggested that soccer match can induce increased concentrations of IL-6, CK, LDH and lactate which may influence on inflammation, muscle damage and fatigue. However, contrast bath and massage were beneficial for recovery from muscle damage.

Identification of mutations in the GNPTA (MGC4170) gene coding for GlcNAc-phosphotransferase α/β subunits in Korean patients with mucolipidosis type II or type IIIA

Paik, Kyung Hoon,Song, Seng Mi,Ki, Chang Seok,Yu, Han-Wook,Kim, Jung Sim,Min, Ki Hoon,Chang, Soo Hee,Yoo, Eun Jae,Lee, In Jung,Kwan, Eun Kyung,Han, Sun Joo,Jin, Dong-Kyu Wiley Subscription Services, Inc., A Wiley Company 2005 Human mutation Vol.26 No.4

<P>Mucolipidosis types II and III are autosomal recessive inherited diseases caused by a deficiency in the lysosomal enzyme N-acetylglucosamine-1 phosphotransferase (GlcNAc-phosphotransferase), which adds phosphate to function as a recognition marker for the uptake and transport of lysosomal enzymes. We investigated mutations in the GNPTA (MGC4170) gene, which codes for the α/β subunits of phosphotransferase, and in the GNPTAG gene, which codes for its γ subunits in five Korean patients with mucolipidosis type II or IIIA. We identified seven mutations in the GNPTA gene, but none in GNPTAG. The mutations in type II patients included p.Q104X (c.310C>T), p.R1189X (c.3565C>T), p.S1058X (c.3173C>G), p.W894X (c.2681G>A), and p.H1158fsX15 (c.3474_3475delTA), all of which are nonsense or frameshift mutations. However, a splicing site mutation, IVS13+1G>A (c.2715+1G>A) was detected along with a nonsense or a frameshift mutation (p.R1189X or p.E858fsX3 (c.2574_2575delGA)) in two mucolipidosis type IIIA patients. This report shows that mutations in the GNPTA gene coding for the α/β subunits of phosphotransferase, and not mutations in the GNPTAG gene, account for most of the genetic mutations found in Korean patients with mucolipidosis type II or IIIA. Hum Mutat 26(4), 308–314, 2005. © 2005 Wiley-Liss, Inc.</P>

The Performance Comparison for the Contention Resolution Policies of the Input-buffered Crosspoint Packet Switch

Paik, Jung-Hoon,Lim, Chae-Tak The Korean Institute of Electrical Engineers 1998 Journal of Electrical Engineering and Information Vol.3 No.1

In this paper, an NxN input-buffered crosspoint packet switch which selects a Head of the Line, HOL, packet in contention randomly is analyzed with a new approach. The approach is based on both a Markov chain representation of the input buffer and the probability that a HOL packet is successfully served. The probability as a function of N is derived, and it makes it possible to express the average packet delay and the average number of packets in the buffer as a function of N. The contention resolution policy based on the occupancy of the input buffer is also presented and analyzed with this same approach and the relationship between two selection policies is analyzed in terms of the occupancy of the input buffer.

Marked Suppression of Ghrelin Concentration by Insulin in Prader-Willi Syndrome

Paik, Kyung-Hoon,Lee, Moon-Kyu,Jin, Dong-Kyu,Kang, Hahn Wook,Lee, Kyung Han,Kim, An Hee,Kim, Cheol,Lee, Ji Eun,Oh, Yoo Joung,Kim, Seonwoo,Han, Sun Joo,Kwon, Eun Kyung,Choe, Yon Ho KOREAN ACADEMY OF MEDICAL SCIENCE 2007 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.22 No.2

<P>The plasma ghrelin has been reported to be elevated in Prader-Willi syndrome (PWS) and modulated by insulin. It was hypothesized that insulin might have a more pronounced effect on reducing plasma ghrelin in PWS patients, which would influence appetite. This study investigated the degree of ghrelin suppression using an euglycemic hyperinsulinemic clamp in children with PWS (n=6) and normal children (n=6). After a 90-min infusion of insulin, the plasma ghrelin level decreased from a basal value of 0.86±0.15 to 0.58±0.12 ng/mL in the controls, and from 2.38±0.76 to 1.12±0.29 ng/mL in children with PWS (<I>p</I>=0.011). The area under the curve below the baseline level over the 90 min insulin infusion was larger in children with PWS than in controls (-92.82±44.4 vs. -10.41±2.87 ng/mL/90 min) (<I>p</I>=0.011). The insulin sensitivity measured as the glucose infusion rate at steady state was similar in the two groups (<I>p</I>=0.088). The decrease in the ghrelin levels in response to insulin was more pronounced in the children with PWS than in the controls. However, the level of ghrelin was always higher in the children with PWS during the clamp study. This suggests that even though insulin sensitivity to ghrelin is well maintained, an increase in the baseline ghrelin levels is characteristic of PWS.</P>