Glycine alleviated diquat-induced hepatic injury via inhibiting ferroptosis in weaned piglets

Hua Hongwei,Xu Xiao,Tian Wei,Li Pei,Zhu Huiling,Wang Wenjun,Liu Yulan,Xiao Kan 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.6

Objective: The beneficial effects of glycine were tested in piglets with diquat-induced hepatic injury. Methods: Thirty-two piglets were assigned by a 2×2 factorial experimental design including glycine supplementation and diquat challenge. After 3 weeks of feeding with a basic diet or a 1% glycine supplemented diet, piglets were challenged with diquat or saline. After 1 week later, the piglets were slaughtered and samples were collected. Results: Our results indicated that glycine alleviated diquat induced morphological hepatic injury, decreased the activities of plasma alanine aminotransferase, aspartate aminotransferase and glutamyl transpeptidase in the piglets under diquat challenge, and increased total antioxidant capacity and antioxidative enzyme activity significantly. Adding glycine enhanced the concentrations of hepatic adenosine triphosphate and adenosine diphosphate. Transmission electron microscope observation showed that diquat induced clear hepatocytes ferroptosis and its effect could be alleviated by glycine to a certain degree. Moreover, glycine significantly affected mRNA and protein expression of ferroptosis-related signals in the liver. Conclusion: These results demonstrated that glycine attenuated liver damage via inhibiting ferroptosis. Objective: The beneficial effects of glycine were tested in piglets with diquat-induced hepatic injury.Methods: Thirty-two piglets were assigned by a 2×2 factorial experimental design including glycine supplementation and diquat challenge. After 3 weeks of feeding with a basic diet or a 1% glycine supplemented diet, piglets were challenged with diquat or saline. After 1 week later, the piglets were slaughtered and samples were collected.Results: Our results indicated that glycine alleviated diquat induced morphological hepatic injury, decreased the activities of plasma alanine aminotransferase, aspartate aminotransferase and glutamyl transpeptidase in the piglets under diquat challenge, and increased total antioxidant capacity and antioxidative enzyme activity significantly. Adding glycine enhanced the concentrations of hepatic adenosine triphosphate and adenosine diphosphate. Transmission electron microscope observation showed that diquat induced clear hepatocytes ferroptosis and its effect could be alleviated by glycine to a certain degree. Moreover, glycine significantly affected mRNA and protein expression of ferroptosis-related signals in the liver.Conclusion: These results demonstrated that glycine attenuated liver damage via inhibiting ferroptosis.

The Specific Case Analysis of Biomineralization Induced by Sulfate Reducing Bacteria

( Hongwei Liu ),( Shuang Qin ),( Chaoyang Fu ),( Fei Xiao ),( Deli Wang ),( Xia Han ),( Tianli Wang ),( Hongfang Liu ) 한국부식방식학회(구 한국부식학회) 2017 Corrosion Science and Technology Vol.16 No.6

The effects of sulfate reducing bacteria (SRB) on the corrosion and scaling of the Q235 carbon steel has been investigated in the simulated sewage water and oil field gathering pipelines production water, using scanning electron microscopy (SEM), energy dispersive x-ray spectrometry (EDS), and three-dimensional stereoscopic microscope. Results indicated that the concentration of SRB reached the maximum value on the ninth day in simulated sewage water with a large amount of scaling on the surface of specimen. In oil field gathering pipelines, a large amount of scaling and mineralization of mineral salts and thick deposition of extracellular polymeric substance (EPS) layers were also observed on the surface of specimen. The thickness of biofilm was about 245 μm within 30 days. After adding microbicides, the thickness of corrosion products film was only up to 48 - 106 μm within 30 days, suggesting that SRB could induce biomineralization. Under-deposit corrosion morphology was uniform in the absence of microbicides while local corrosion was observed in the presence of microbicides.

pH-sensitive drug controlled release core/shell fibers fabricated by combination of electrospinning and photopolymerization

Xiao-lei Zhu,Hongwei Zhang,Jun Nie,Guiping Ma 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.45 No.-

pH-sensitive drug loaded core shellfibers were fabricated by a combination of electrospinning and UVphoto-polymerization. Combretastatin A4 (CA4) was selected as the model drug loaded in PLA to test thepH-sensitivity property of the core shellfibers. The morphology of thefibers was studied by scanningelectron microscopy (SEM), and the core shell structure of thefibers was confirmed by transmissionelectron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS). The drug release assay wastested via the UV–vis spectrophotometer, and the pH-sensitivity of core shellfibers was tested by drugrelease assay under pH 5.0 and 7.4.

The progresses of superconducting technology for power grid last decade in China

Xiao, Liye,Gu, Hongwei The Korea Institute of Applied Superconductivity a 2015 한국초전도저온공학회논문지 Vol.17 No.1

With the increasing development of renewable energy, it is expected that large-scale renewable power would be transported from the west and north area of China to the east and south area. For this reason, it will be necessary to develop a wide-area power grid in which the renewable energy would be the dominant power source, and the power grid will be faced by some critical challenges such as long-distance large-capacity power transmission, the stability of the wide-area power grid and the land use problem for the power grid. The superconducting technology for power (STP) would be a possible alternative for the development of China's future power grid. In last decade, STP has been extensively developed in China. In this paper, we present an overview of the R&D of STP last decade in China including: 1) the development of high temperature superconducting (HTS) materials, 2) DC power cables, 3) superconducting power substations, 4) fault current limiters and 5) superconducting magnetic energy storage (SMES).

The progresses of superconducting technology for power grid last decade in China

Liye Xiao,Hongwei Gu 한국초전도저온학회 (구 한국초전도저온공학회) 2015 한국초전도저온공학회논문지 Vol.17 No.1

Screening of Recurrence Related MicroRNA in Ductal Carcinoma In Situ and Functional Study of MicroRNA-654-5p

Shi Li,Tianjie Pu,Lin Xiao,Hongwei Gao,Li Li,Feng Ye,Yueping Liu,Hong Bu 한국유방암학회 2019 Journal of breast cancer Vol.22 No.1

Purpose: Ductal carcinoma in situ (DCIS) contributes to 20%–30% of newly diagnosed cases of breast cancer in China. Although the breast cancer-specific mortality of DCIS is extremely low, a small proportion of DCIS patients still show relapse or metastasis, leading to poor prognosis. Little is known about the molecular mechanism for DCIS metastasis, partly due to the limited number of poor prognosis patients. This study analyzed the clinicopathological features and screened key microRNAs (miRNAs) contributing to local or distant recurrence. Methods: The clinicopathological features of DCIS were evaluated and survival analysis were performed to clarify risk factors associated with poor prognosis. Using miRNA arrays and real-time quantitative polymerase chain reaction (RT-qPCR) on DCIS formalin-fixed and paraffin-embedded samples with or without microinvasion with different clinical outcomes, potential DCIS metastasis-related miRNAs were screened out and further validated. The influence of one identified miRNA, miRNA-654-5p, on DCIS progression was analyzed. Results: Poor prognosis was significantly associated with larger tumor size and higher lymph node metastasis rate (both p < 0.05). Both were independent prognostic factors for DCIS. According to RT-qPCR results, distinct miRNA expression profiles were identified between DCIS and DCIS with microinvasion (DCIS-Mi) patients. In the DCIS panel, miRNA-654-5p was significantly upregulated in the patients with poor prognosis. In vitro, miRNA-654-5p promoted MDA-MB-231 cell mobility in healing tests and metastasis in the Transwell study. Conclusion: The panel of high-risk miRNAs in DCIS and DCIS-Mi differs markedly. miRNA-654-5p is significantly upregulated DCIS patients having poor prognosis and may be essential for local and distant recurrence in DCIS.

A Reliability Evaluation Model for the Power Devices Used in Power Converter Systems Considering the Effect of the Different Time Scales of the Wind Speed Profile

Haiting Ji,Hui Li,Yang Li,Li Yang,Guoping Lei,Hongwei Xiao,Jie Zhao,Lefeng Shi 전력전자학회 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.2

This paper presents a reliability assessment model for the power semiconductors used in wind turbine power converters. In this study, the thermal loadings at different timescales of wind speed are considered. First, in order to address the influence of long-term thermal cycling caused by variations in wind speed, the power converter operation state is partitioned into different phases in terms of average wind speed and wind turbulence. Therefore, the contributions can be considered separately. Then, in regards to the reliability assessment caused by short-term thermal cycling, the wind profile is converted to a wind speed distribution, and the contribution of different wind speeds to the final failure rate is accumulated. Finally, the reliability of an actual power converter semiconductor for a 2.5 MW wind turbine is assessed, and the failure rates induced by different timescale thermal behavior patterns are compared. The effects of various parameters such as cut-in, rated, cut-out wind speed on the failure rate of power devices are also analyzed based on the proposed model.

Association of UDP-galactose-4-epimerase with milk protein concentration in the Chinese Holstein population

Li Cong,Cai Wentao,Liu Shuli,Zhou Chenghao,Cao Mingyue,Yin Hongwei,Sun Dong-Xiao,Zhang Shengli,Loor Juan J. 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.11

Objective: An initial RNA-Sequencing study revealed that UDP-galactose-4-epimerase (GALE) was one of the most promising candidates for milk protein concentration in Chinese Holstein cattle. This enzyme catalyzes the interconversion of UDP-galactose and UDP-glucose, an important step in galactose catabolism. To further validate the genetic effect of GALE on milk protein traits, genetic variations were identified, and genotypes-phenotypes associations were performed. Methods: The entire coding region and the 5’-regulatory region (5’-UTR) of GALE were re-sequenced using pooled DNA of 17 unrelated sires. Association studies for five milk production traits were performed using a mixed linear animal model with a population encompassing 1,027 Chinese Holstein cows. Results: A total of three variants in GALE were identified, including two novel variants (g.2114 A>G and g.2037 G>A) in the 5’-UTR and one previously reported variant (g.3836 G>C) in an intron. All three single nucleotide polymorphisms (SNPs) were associated with milk yield (p<0.0001), fat yield (p = 0.0006 to <0.0001), protein yield (p = 0.0232 to <0.0001) and protein percentage (p<0.0001), while no significant associations were detected between the SNPs and fat percentage. A strong linkage disequilibrium (D’ = 0.96 to 1.00) was observed among all three SNPs, and a 5 Kb haplotype block involving three main haplotypes with GAG, AGC, and AGG was formed. The results of haplotype association analyses were consistent with the results of single locus association analysis (p<0.0001). The phenotypic variance ratio above 3.00% was observed for milk protein yield that was explained by SNP-g.3836G >C. Conclusion: Overall, our findings provided new insights into the polymorphic variations in bovine GALE gene and their associations with milk protein concentration. The data indicate their potential uses for marker-assisted breeding or genetic selection schemes.