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Hongli Cao,Ping Zhang,Hong Yu,Jianing Xi 한국분자세포생물학회 2022 Molecules and cells Vol.45 No.6
Extracellular vesicles (EVs) play an essential role in the communication between cells and the tumor microenvironment. However, the effect of tumor-derived EVs on the growth and metastasis of lung adenocarcinoma (LUAD) remains to be explored. This study aimed to elucidate the role of miR-153-3p-EVs in the invasion and migration capabilities of LUAD cells and explore its mechanism through in vivo and in vitro experiments. We found that miR-153-3p was specifically and highly expressed in LUAD and its secreted EVs. Furthermore, the expression of BANCR was negatively regulated by miR-153-3p and identified as a target gene of miR-153-3p using luciferase reporter assays. Through further investigation, we found that the downregulation of BANCR activates the PI3K/AKT pathway and accelerates the process of epithelial-mesenchymal transition (EMT), which ultimately leads to the aggravation of LUAD. The orthotopic xenograft mouse model was established to illustrate the effect of miR-153-3p-EVs on LUAD. Animal studies showed that miR-153-3p-EVs accelerated tumor growth in mice. Besides, we found that miR-153-3p-EVs could damage the respiratory ability of mice and produce a mass of inflammatory cells around the lung tissue of mice. Nevertheless, antagomir-153-3p treatment could inhibit the deterioration of respiratory function and inhibit the growth of lung tumors in mice. In conclusion, our study reveals the potential molecular mechanism of miR-153-3p-EVs in the development of LUAD and provides a potential strategy for the treatment of LUAD.
Cao Hongli,Zhang Guosheng,Ma Hui,Xue Zhongwen,Huo Ran,Wang Kun,Liu Zijin 한국미생물·생명공학회 2024 Journal of microbiology and biotechnology Vol.34 No.1
Refractory infections, such as hospital-acquired pneumonia, can be better diagnosed with the assistance of precise methicillin-resistant Staphylococcus aureus (MRSA) testing. However, traditional methods necessitate high-tech tools, rigorous temperature cycling, and the extraction of genetic material from MRSA cells. Herein, we propose a sensitive, specific, and extraction-free strategy for MRSA detection by integrating allosteric probe-based target recognition and exonuclease-III (ExoIII)-enhanced color reaction. The penicillin-binding protein 2a (PBP2a) aptamer in the allosteric probe binds with MRSA to convert protein signals to nucleic acid signals. This is followed by the DNA polymerase-assisted target recycle and the production of numerous single-strand DNA (ssDNA) chains which bind with silver ion (Ag+ ) aptamer to form a blunt terminus that can be identified by Exo-III. As a result, the Ag+ aptamer pre-coupled to magnetic nanoparticles is digested. After magnetic separation, the Ag+ in liquid supernatant catalyzes 3,3’,5,5’-tetramethylbenzidine (TMB) for a color reaction. In addition, a concentration of 54 cfu/mL is predicted to be the lowest detectable value. Based on this, our assay has a wide linear detection range, covering 5 orders of magnitude and demonstrating a high specificity, which allows it to accurately distinguish the target MRSA from other microorganisms.
Cao, Rui,Wu, Wang Jun,Zhou, Xiao Long,Xiao, Peng,Wang, Yi,Liu, Hong Lin Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.4
Most follicles in the mammalian ovary undergo atresia. Granulosa cell apoptosis is a hallmark of follicle atresia. Our previous study using a microRNA (miRNA) microarray showed that the let-7 microRNA family was differentially expressed during follicular atresia. However, whether the let-7 miRNA family members are related to porcine (Sus scrofa) ovary follicular apoptosis is unclear. In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine ovary follicles compared with healthy follicles, while let-7g was highly expressed during follicle atresia. Furthermore, flow cytometric analysis and Hoechst33342 staining demonstrated that let-7g increased the apoptotic rate of cultured granulosa cells. In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways. Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.
Hongli Zhang,Ying Guo,Zhaoqin Cao 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.5
This paper is concerned with the aperiodically intermittent pinning control (AIPC) for the synchronization of nonlinear coupled networks, where both the internal time delay and the coupling time delay are considered. We consider the aperiodicity of AIPC from the average sense. Then by extending the Halanay’s inequality to the case with two kinds different delays, we obtain two kinds of synchronization criteria: one is the upper bound of time delay must be less than the average control width but can be larger than certain control widths; the other is that the upper bound of time delay has no relationship with the average control width. We show that the two criteria lead to complementary results. The effectiveness of our results is confirmed by numerical simulations.
CAO Yu,CHEN Xiao-hong,MA Yue-ru 인하대학교 정석물류통상연구원 2009 인하대학교 정석물류통상연구원 학술대회 Vol.2009 No.10
The correct judgment of the stage at which a corporation stays is the premise of empirical research on the issue of a corporation in the life cycle perspective, this paper has put forward two methods, by which the evolutionary model of our national corporate competitiveness at different stage of life cycle has been verified with empirical research, one is acquired directly from questionare, the other is the employ of fuzzy-integrated judgment through setting up relative subjects. This has in one hand. proved the feasibility of different life cycle judgment approach adopted by this paper, and on the other hand, verified the reliability of conduction made by this paper on the evolutionary model of our national corporate competitiveness. The result reveals that the life cycle of our national corporations can all be assessed by these two methods. By these two methods, this paper has put forward the evolutionary model of our national corporate competitiveness at different stage of life cycle, which is of significant referential importance of the establishment of corporate competitive strategy
Development of Multidirectional Nonlinear Numerical Wave Tank by Naoe-FOAM-SJTU Solver
Cao, Hong-Jian,Wan, De-Cheng Korean Society of Ocean Engineers 2014 International journal of ocean system engineering Vol.4 No.1
A three-dimensional multidirectional nonlinear numerical wave tank (NWT) based on the Navier-Stokes equations and the Finite Volume Method (FVM) is developed by using the two-phase hydrodynamic flow solver naoe-FOAM-SJTU based on the open source toolbox OpenFOAM. The free surface is capturing with the Volume Of Fluids (VOF). The directional wave including Stokes wave, solitary wave and nonlinear wave are simulated and verified. The multi-directional waves are also simulated with particular wave spectral such as JONSWAP and wave directional spreading function. The obtained numerical results show the capability of the solver to generate different type of multidirectional nonlinear waves accurately. Meanwhile, it implies that the presented NWT can easily extend to model the wave-structures interactions, which will be great help to the offshore structures design.
Development of Multidirectional Nonlinear Numerical Wave Tank by Naoe-FOAM-SJTU Solver
Cao, Hong-Jian,Wan, De-Cheng Korean Society of Ocean Engineers 2015 Journal of advanced research in ocean engineering Vol.1 No.1
A three-dimensional multidirectional nonlinear numerical wave tank (NWT) based on the Navier-Stokes equations and the Finite Volume Method (FVM) is developed by using the two-phase hydrodynamic flow solver naoe-FOAM-SJTU based on the open source toolbox OpenFOAM. The free surface is capturing with the Volume Of Fluids (VOF). The directional wave including Stokes wave, solitary wave and nonlinear wave are simulated and verified. The multi-directional waves are also simulated with particular wave spectral such as JONSWAP and wave directional spreading function. The obtained numerical results show the capability of the solver to generate different type of multidirectional nonlinear waves accurately. Meanwhile, it implies that the presented NWT can easily extend to model the wave-structures interactions, which will be great help to the offshore structures design.