Protective effect of Lactobacillus plantarum ATCC8014 on acrylamide-induced oxidative damage in rats

Zhao Sijia,Zhao Xiaoduo,Liu Qingbo,Jiang Yujun,Li Yanhua,Feng Wenxiao,Xu Honghua,Shao Meili 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.4

Acrylamide (AA), which is mainly found in fried foods, causes neurotoxicity, genetic toxicity, carcinogenic effects, and DNA damage. This study confirms that a strain of lactic acid bacteria (Lactobacillus plantarum ATCC8014) could alleviate the toxicity of rats by inhibiting the AA-induced oxidative damage. Forty-eight adult male SD rats were randomly divided into eight groups: control group, AA group (40 mg/kg), three different doses (1 × 107 CFU/ml, 1 × 108 CFU/ ml, 1 × 109 CFU/ml of Lactobacillus plantarum ATCC8014) of prevention groups and therapeutic groups, respectively. At the end of three-week experiment, AA treatment produced a significant reduction in the rate of weight gain along with the symptoms of hind limb splay and ataxia. Histological examinations revealed various degrees of injury in five tissues. Levels of superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) in group AA rats were significantly decreased, but the level of lipid peroxidation (LPO) was significantly increased (p < 0.05). Both prevention and therapeutic groups with 1 × 109 CFU/ml of Lactobacillus plantarum ATCC8014 could effectively reduce the injury of AA to the body. However, reductions in both groups were not statistically significant.

Effects of Chemical Stabilizers on an Expansive Clay

Honghua Zhao,Louis Ge,Thomas M. Petry,Yi-Zhen Sun 대한토목학회 2014 KSCE JOURNAL OF CIVIL ENGINEERING Vol.18 No.4

Expansive clays exhibit a high potential for the volume change behavior when the soil moisture changes. The estimated annualcost of the damage to the facilities built on expansive clay in the United States exceeds 9 billion dollars. Because of the physicochemicalnature of the expansive clay, the use of chemical agents to improve their engineering properties is common. This researchinvestigated the effects of chemical agents on an expansive soil from Texas through a laboratory injection method. The agents used inthis study included lime, potassium based agents, and a group of ionic agents. Swelling tests, chemical tests, and soil suction testswere used to evaluate the stabilizing effects of those chemical agents. The testing results indicated that potassium based stabilizer, isan effective stabilizing agent to control the swelling potential of the expansive clay. It can also be injected in the field to build amoisture barrier. The chemical tests on the injected Texas clay showed that the stabilizing mechanism of the ionic agents is possiblythrough the cations’ exchange and the increase of the cations’ concentrations in the soil pore water.

Cultural Connotation and Developmental Status of Chinese Boccaro Tea Set

Zhao Yanhong,Han Yuying,Zhang Honghua 한국차학회 2015 한국차학회지 Vol.- No.S

Boccaro Teapot is one of the main carriers of tea culture in China. This paper introduces the material and its applied characteristics, modelling, development history, appreciation, and cultural connotation of boccaro tea sets.This article mainly introduced some classical works of Chinese boccaro artists. The production characteristics, display form and artistic connotation are recommended. Chinese boccaro teapots are both practical tea sets and works of art, they fully conveys the culture connotation of tea culture. The author thinks the main connotation of tea culture is to respect and tolerate, hopes people’s the positive attitude and wishes to create a harmonious happy life together. Since the advent of boccaro teapots, they are popular all over the world, especially loved and collected by people from mainland China, Taiwan, Hong Kong, South Korea, Japan and Southeast Asian countries

A brain somatic RHEB doublet mutation causes focal cortical dysplasia type II

Shanshan Zhao,Zhenghui Li,Muxian Zhang,Lingliang Zhang,Honghua Zheng,Jinhuan Ning,Yanyan Wang,Feng-Peng Wang,Xiaobin Zhang,Hexia Gan,Yuanqing Wang,Xian Zhang,Hong Luo,Guojun Bu,Huaxi Xu,Yi Yao,Yun-wu 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Focal cortical dysplasia type II (FCDII) is a cerebral cortex malformation characterized by local cortical structure disorganization, neuronal dysmorphology, and refractory epilepsy. Brain somatic mutations in several genes involved in the PI3K/AKT/mTOR pathway are associated with FCDII, but they are only found in a proportion of patients with FCDII. The genetic causes underlying the development FCDII in other patients remain unclear. Here, we carried out whole exome sequencing and targeted sequencing in paired brain–blood DNA from patients with FCDII and identified a brain somatic doublet mutation c.(A104T, C105A) in the Ras homolog, mTORC1 binding (RHEB) gene, which led to the RHEB p.Y35L mutation in one patient with FCDII. This RHEB mutation carrier had a dramatic increase of ribosomal protein S6 phosphorylation, indicating mTOR activation in the region of the brain lesion. The RHEB p.Y35L mutant protein had increased GTPλS-binding activity compared with wild-type RHEB. Overexpression of the RHEB p. Y35L variant in cultured cells also resulted in elevated S6 phosphorylation compared to wild-type RHEB. Importantly, in utero electroporation of the RHEB p.Y35L variant in mice induced S6 phosphorylation, cytomegalic neurons, dysregulated neuron migration, abnormal electroencephalogram, and seizures, all of which are found in patients with FCDII. Rapamycin treatment rescued abnormal electroencephalograms and alleviated seizures in these mice. These results demonstrate that brain somatic mutations in RHEB are also responsible for the pathogenesis of FCDII, indicating that aberrant activation of mTOR signaling is a primary driver and potential drug target for FCDII.

A New Spatial Deformation Measurement Method Using 3D Reconstruction Technology during Pile Penetration

Cong Liu,Xiaowei Tang,Huanwei Wei,Honghua Zhao 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.11

Non-intrusive observation of the spatial deformation field of the soil has been a difficult problem for model test measurements. Based on the advantage that the visualization test method of transparent soil can observe the two-dimensional (2D) deformation inside the soil, this study proposes a new automatic tomographic scanning measuring device to observe the three-dimensional (3D) spatial soil deformation inside the transparent soil model. A series of 2D laser speckle images of different vertical cross sections before and after deformation were obtained, and an improved 3D reconstruction algorithm was used to reconstruct the 3D displacement field of soil after deformation. Different types of jacked-pile penetration model tests were carried out to investigate the spatial disturbance of the soil around the pile caused by the squeezing effect of the jacked-pile. The test results showed that the developed novel automatic tomographic scanning measuring device with the modified 3D reconstruction procedure could be an innovative tool in geotechnical physical model experiments. The model test results visually revealed the mechanism of the soil squeezing effect of jacked-piles with different pile head forms. Moreover, the spatial disturbance effect caused by different penetration stages was also discussed herein.

Model Tests of Jacked-Pile Penetration into Sand Using Transparent Soil and Incremental Particle Image Velocimetry

Cong Liu,Xiao-Wei Tang,Huanwei Wei,Pengpeng Wang,Honghua Zhao 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.4

This paper presents a series of physical modelling tests of jacked-pile penetration into sand, combining the transparent soil and incremental reliability-guided particle image velocimetry, which provides a non-intrusively internal deformation measurement approach to monitor the internal movement of soil caused by jacked-pile penetration. The difference between the full-model test and the half-model test was analyzed quantitatively for the first time. This paper aims to provide some new insight into the penetration mechanisms of different shapes of pile tip. In addition, the phenomenon of meta-stable sand plug in the process of jacked-pile penetration was studied by means of the method of partial replacement with dyed particles. Experimental evidence has shown that the boundary and interaction effect causes the experimental phenomenon of the half-model test to be different from the true condition. The shape effect of pile tip on the governing penetration mechanisms was discussed, and the disturbance effect of different shapes of pile tip was evaluated and compared based on a large number of model tests and published literatures. The paper intuitively demonstrates that the different penetration mechanism of flat-ended pile and cone-ended pile is mainly due to the formation of the meta-stable sand plug beneath the pile tip.