Variable Coefficient Inductance Model-Based Four-Quadrant Sensorless Control of SRM

Song-Yan Kuai,Xue-Feng Li,Xing-Hong Li,Jinyang Ma 전력전자학회 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.6

The phase inductance of a switch reluctance motor (SRM) is significantly nonlinear. With different saturation conditions, the phase inductance shape is clearly changed. This study focuses on the relationship between coefficient and current in an inductance model with ignored harmonics above the order of 3. A position estimation method based on the variable coefficient inductance model is proposed in this paper. A four-quadrant sensorless control system of the SRM drive is constructed based on the relationship between variable coefficient inductance and rotor position. The proposed algorithms are implemented in an experimental SRM test setup. Experimental results show that the proposed method estimates position accurately in operating two/four-quadrants. The entire system also has good static and dynamic performance.

Effects of SRBSDV-infected rice plants on the fitness of vector and non-vector rice planthoppers

Hong-Xing Xu,Hong-Xing Xu,Xu-Song Zheng,Yajun Yang,Jue-Feng Zhang Zhang,Zhong-Xian Lu 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3

Southern rice black-streaked dwarf virus (SRBSDV) is a new member of the genus Fijivirus and transmitted by whitebacked planthopper (WBPH), Sogatella furcifera (Horváth). The rice area affected by SRBSDV and the subsequent damages are increasing rapidly. In current study, we evaluated the effects of SRBSDV-infected plants on the ecological fitness and the tolerance to adverse environmental factors of vector WBPH and non-vector brown planthopper (BPH), Nilaparvata lugens (Stål). Our study revealed that the fitness of WBPH and BPH were both influenced by feeding on SRBSDV-infected rice. Fecundity ofWBPH feeding on the SRBSDV-infected rice plants were higher than those on healthy plants, nymphal duration of male WBPH prolonged markedly by 0.77 d (P b 0.05), while the weight of brachypterous female adults significantly decreased (P b 0.05), and the average survival time shortened both at 26 °C and 31 °C, respectively. Median lethal time of WBPH on SRBSDV-infected and healthy rice plants were 80 h and 112 h at 26 °C, 64 h and 88 h at 31 °C, respectively. As for non-vector BPH, nymph survival rate decreased significantly by 11.22% (P b 0.05) and the longevity of female adults also shortened (P b 0.05), while hatchability of BPH eggs increased (P b 0.05). Survival time of BPH feeding on healthy rice at 26 °Cwas significant longer than those feeding on healthy and infected rice at 31 °C. The above results imply that the vectorWBPH suffers much stronger effects than non-vector BPH fromfeeding on SRBSDVinfected rice plants.

Can CD44+/CD24- Tumor Cells Be Used to Determine the Extent of Breast Cancer Invasion Following Neoadjuvant Chemotherapy?

Hong Wu,Ruhui Li,XiaoDong Hang,Ming Yan,Feng Niu,Lidi Liu,Wei Liu,Song Zhao,Shaokun Zhang 한국유방암학회 2011 Journal of breast cancer Vol.14 No.3

Purpose: To investigate the distribution of CD44+/CD24- cells in breast cancers in relation to tumor size before and after the administration of neoadjuvant chemotherapy. Methods: CD44+/CD24- tumor cells obtained from breast cancer specimens were characterized in vivo and in vitro using tumor formation assays and mammosphere generation assays, respectively. The distribution of CD44+/CD24- tumor cells in 78 breast cancer specimens following administration of neoadjuvant chemotherapy was also evaluated using immunofluorescence assays, and this distribution was compared with the extent of tumor invasion predicted by Response Evaluation Criteria in Solid Tumours (RECIST). Results: In 27/78 cases, complete remission (CR) was identified using RECIST. However, 18 of these CR cases were associated with a scattered distribution of tumor stem cells in the outline of the original tumor prior to neoadjuvant chemotherapy. After neoadjuvant chemotherapy, 24 cases involved cancer cells that were confined to the tumor outline, and 21 cases had tumor cells or tumor stem cells overlapping the tumor outline. In addition, there were 6 patients who were insensitive to chemotherapy, and in these cases, both cancer cells and stem cells were detected outside the contours of the tumor volume imaged prior to chemotherapy. Conclusion:CD44+/CD24- tumor cells may be an additional parameter to evaluate when determining the extent of breast cancer invasion.

PU.1 Is Identified as a Novel Metastasis Suppressor in Hepatocellular Carcinoma Regulating the miR-615-5p/IGF2 Axis

Song, Li-Jie,Zhang, Wei-Jie,Chang, Zhi-Wei,Pan, Yan-Feng,Zong, Hong,Fan, Qing-Xia,Wang, Liu-Xing Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9

Invasion and metastasis is the major cause of tumor recurrence, difficulty for cure and low survival rate. Excavating key transcription factors, which can regulate tumor invasion and metastasis, are crucial to the development of therapeutic strategies for cancers. PU.1 is a master hematopoietic transcription factor and a vital regulator in life. Here, we report that, compared to adjacent non-cancerous tissues, expression of PU.1 mRNA in metastatic hepatocellular carcinoma (HCC), but not primary HCC, was significantly down-regulated. In addition, levels of PU.1 mRNA in metastatic hepatoma cell lines MHCC97L and MHCC97H were much lower than in non-metastatic Hep3B cells. Transwell invasion assays after PU.1 siRNA transfection showed that the invasion of hepatoma cell lines was increased markedly by PU.1 knockdown. Oppositely, overexpression of PU.1 suppressed the invasion of these cells. However, knockdown and overexpression of PU.1 did not influence proliferation. Finally, we tried to explore the potential mechanism of PU.1 suppressing hepatoma cell invasion. ChIP-qPCR analysis showed that PU.1 exhibited a high binding capacity with miR-615-5p promoter sequence. Overexpression of PU.1 caused a dramatic increase of pri-, pre- and mature miR-615-5p, as well as a marked decrease of miR-615-5p target gene IGF2. These data indicate that PU.1 inhibits invasion of human HCC through promoting miR-615-5p and suppressing IGF2. These findings improve our understanding of PU.1 regulatory roles and provided a potential target for metastatic HCC diagnosis and therapy.

A rice ABC transporter, OsABCC1, reduces arsenic accumulation in the grain

Song, Won-Yong,Yamaki, Tomohiro,Yamaji, Naoki,Ko, Donghwi,Jung, Ki-Hong,Fujii-Kashino, Miho,An, Gynheung,Martinoia, Enrico,Lee, Youngsook,Ma, Jian Feng National Academy of Sciences 2014 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.111 No.44

<P><B>Significance</B></P><P>Contamination of water and foods with arsenic (As) poses a threat to millions of people worldwide. Because the rice grain is the major source of As intake, reducing the transfer of As from soil to the grain is a pressing public health issue. We found that a member of the <I>Oryza sativa</I> C-type ATP-binding cassette transporter (OsABCC) family, OsABCC1, detoxifies As and reduces the amount of As in the rice grain. OsABCC1 in the upper nodes of rice plants restricts the distribution of As to the grain by sequestering it in the vacuoles of the phloem companion cells of diffuse vascular bundles directly connected to the grain. Our work suggests a strategy for limiting As accumulation in rice grains and thereby reducing human As exposure.</P><P>Arsenic (As) is a chronic poison that causes severe skin lesions and cancer. Rice (<I>Oryza sativa</I> L.) is a major dietary source of As; therefore, reducing As accumulation in the rice grain and thereby diminishing the amount of As that enters the food chain is of critical importance. Here, we report that a member of the <I>Oryza sativa</I> C-type ATP-binding cassette (ABC) transporter (OsABCC) family, OsABCC1, is involved in the detoxification and reduction of As in rice grains. We found that <I>OsABCC1</I> was expressed in many organs, including the roots, leaves, nodes, peduncle, and rachis. Expression was not affected when plants were exposed to low levels of As but was up-regulated in response to high levels of As. In both the basal nodes and upper nodes, which are connected to the panicle, OsABCC1 was localized to the phloem region of vascular bundles. Furthermore, OsABCC1 was localized to the tonoplast and conferred phytochelatin-dependent As resistance in yeast. Knockout of <I>OsABCC1</I> in rice resulted in decreased tolerance to As, but did not affect cadmium toxicity. At the reproductive growth stage, the As content was higher in the nodes and in other tissues of wild-type rice than in those of <I>OsABCC1</I> knockout mutants, but was significantly lower in the grain. Taken together, our results indicate that OsABCC1 limits As transport to the grains by sequestering As in the vacuoles of the phloem companion cells of the nodes in rice.</P>

Therapeutic Regimens and Prognostic Factors of Brain Metastatic Cancers

Song, Wen-Guang,Wang, Yi-Feng,Wang, Rui-Lin,Qu, Yin-E,Zhang, Zhi,Li, Guo-Zhong,Xiao, Ying,Fang, Fang,Chen, Hong Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Objective: This work aims to investigate the therapeutic regimen of brain metastatic cancers and the relationship between clinical features and prognosis. Methods: Clinical data of 184 patients with brain metastatic cancers were collected and analysed for the relationship between survival time and age, gender, primary diseases, quantity of brain metastatic foci, their position, extra cranial lesions, and therapeutic regimens. Results: The average age of onset was 59.1 years old. The median survival time (MST) was 15.0 months, and the patients with breast cancer as the primary disease had the longest survival time. Females had a longer survival time than males. Patients with meningeal metastasis had extremely short survival time. Those with less than 3 brain metastatic foci survived longer than patients with more than 3. The MST of patients receiving radiotherapy only and the patients receiving chemotherapy only were all 10.0 months while the MST of patients receiving combination therapy was 16.0 months. Multiple COX regression analysis demonstrated that gender, primary diseases, and quantity of brain metastatic foci were independent prognostic factors for brain metastatic cancers. Conclusions: Chemotherapy is as important as radiotherapy in the treatment of brain metastatic cancer. Combination therapy is the best treatment mode. Male gender, brain metastatic cancers originating in the gastrointestinal tract, more than 3 metastatic foci, and involvement of meninges indicate a worse prognosis.

Identification and Functional Analysis of Differentially Expressed Genes Related to Metastatic Osteosarcoma

Niu, Feng,Zhao, Song,Xu, Chang-Yan,Chen, Lin,Ye, Long,Bi, Gui-Bin,Tian, Gang,Gong, Ping,Nie, Tian-Hong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Background: To explore the molecular mechanisms of metastatic osteosarcoma (OS) by using the microarray expression profiles of metastatic and non-metastatic OS samples. Materials and Methods: The gene expression profile GSE37552 was downloaded from Gene Expression Omnibus database, including 2 human metastatic OS cell line models and 2 two non-metastatic OS cell line models. The differentially expressed genes (DEGs) were identified by Multtest package in R language. In addition, functional enrichment analysis of the DEGs was performed by WebGestalt, and the protein-protein interaction (PPI) networks were constructed by Hitpredict, then the signal pathways of the genes involved in the networks were performed by Kyoto Encyclopaedia of Genes and Genomes (KEGG) automatic annotation server (KAAS). Results: A total of 237 genes were classified as DEGs in metastatic OS. The most significant up- and down-regulated genes were A2M (alpha-2-macroglobulin) and BCAN (brevican). The DEGs were significantly related to the response to hormone stimulus, and the PPI network of A2M contained IL1B (interleukin), LRP1 (low-density lipoprotein receptor-related protein 1) and PDGF (platelet-derived growth factor). Furthermore, the MAPK signaling pathway and focal adhesion were significantly enriched. Conclusions: A2M and its interactive proteins, such as IL1B, LRP1 and PDGF may be candidate target molecules to monitor, diagnose and treat metastatic OS. The response to hormone stimulus, MAPK signaling pathway and focal adhesion may play important roles in metastatic OS.

Expression and Effects of JMJD2A Histone Demethylase in Endometrial Carcinoma

Wang, Hong-Li,Liu, Mei-Mei,Ma, Xin,Fang, Lei,Zhang, Zong-Feng,Song, Tie-Fang,Gao, Jia-Yin,Kuang, Ye,Jiang, Jing,Li, Lin,Wang, Yang-Yang,Li, Pei-Ling Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

Previous studies have demonstrated that JMJD2A is a potential oncogene and is overexpressed in human tumors. However, its role in the endometrial carcinoma remains largely unknown. In this study, we discovered that JMJD2A was overexpressed in endometrial carcinoma, using immunohistochemistry, quantitative realtime polymerase chain reaction, and western blotting. Downregulation of JMJD2A led to reduced endometrial carcinoma RL95-2 and ISK cell proliferation, invasion and metastasis as asessed with cell counting kit-8, cell migration and invasive assays. Collectively, our results support that JMJD2A is a promoter of endometrial carcinoma cell proliferation and survival, and is a potential novel drug target.

Patterns of Failure and Survival Trends in 3,808 Patients with Stage II Nasopharyngeal Carcinoma Diagnosed from 1990 to 2012: A Large-Scale Retrospective Cohort Study

Xue-Song Sun,Di-Han Liu,Sai-Lan Liu,Qiu-Yan Chen,Shan-Shan Guo,Yue-Feng Wen,Li-Ting Liu,Hao-Jun Xie,Qing-Nan Tang,Yu-Jing Liang,Xiao-Yun Li,Jin-Jie Yan,Ming-Huang Hong,Jun Ma,Lin-Quan Tang,Hai-Qiang M 대한암학회 2019 Cancer Research and Treatment Vol.51 No.4

Purpose The purpose of this study was to investigate the survival trends and patterns of failure in patients with stage II nasopharyngeal carcinoma (NPC) treated with radiotherapy (RT) and chemotherapy over the last 20 years. Materials and Methods Thirty-eight hundred and eight patients diagnosed with stage II NPC between January 1990 and December 2012 were involved in this retrospective cohort study. All patients were treated with RT. According to the main imaging techniques and RT technology, we categorized these patients into four calendar periods: 1990-1996, 1997-2002, 2003-2007, and 2008-2012. Overall survival (OS), progression-free survival (PFS), locoregional relapse-free survival (LRFS), and distant metastasis–free survival (DMFS) were served as the clinical outcome. Results After a median follow-up period of 84.7 months, we observed increasing trends in survival and disease control. The 3- and 5-year OS rates increased from 87.1% and 78.7% in the first calendar period to 97.4% and 94.5% in the last calendar period, respectively (p < 0.001). Additionally, significant increasing trends could be seen in the PFS and LRFS during the four calendar periods. In the subgroup analysis, the LRFS in patients older than 50 years at diagnosis showed greater improvement than younger patients. However, the rate of distant metastasis was stable and relatively low, as the 5-year DMFS ranged from 90.5% to 94.7% among the four calendar periods. Conclusion The survival rates in patients with stage II NPC showed increasing trends from 1990 to 2012. The advance of RT provided excellent locoregional control and enhanced OS.

Three New 29 Carbon Skeletons Pentacyclic Triterpenoids and S-equol from Biogas Slurry

Jian-Feng Xu,Hui-Bin Wu,Ding-Cai Liu,Long Sha,Wen-Hui Wu,Hua Fan,Yishan Song,Hong-Guang Zhu 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.12

Bioactive natural products were firstly obtained from biogas slurry. Three new 29 carbon skeletons of the pentacyclic triterpenoid compounds 24-norolean-12-ene-3,22-dione (1), 3β-hydroxy-24-norolean-12-ene-22-one (2), 3α-hydroxy-24-norolean-12-ene-22-one (3), as well as one known compound S-equol (4) were isolated and purified from the MeOH extract of chicken manure biogas slurry. The molecular structures of the four compounds were elucidated based on the extensive spectroscopic data analysis, and the structure of Compound 1 was further confirmed by single-crystal X-ray diffraction. The structures of Compounds 1, 2, and 3 are similar with oleanolicum and hederagenin that has excellent anti-tumor activities. The cytotoxicity against five cancer cell lines (Hela, A549, MCF7, PC3, and B16) of Compounds 1–4 was tested. Similar to Compound 4 (S-equol), Compounds 1–3 (pentacyclic triterpenoids) showed cytotoxicity activity against different tumor cell lines. Compounds 1–3 showed slightly lower cytotoxicity activities than Compound 4. The IC50 of Compound 4 was determined to be 9.7–27.6 μM, while the IC50 values of 1–3 were 17.6–65.3 μM. There are no significant differences in the cytotoxicity capacities between Compounds 1, 2, and 3.