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Exposure Level of Airborne Bacteria in the University Laboratories in Seoul, Korea
Hwang, Sung-Ho,Yoo, Kyong-Nam,Park, Ji-Ho,Park, Dong-Uk,Yoon, Chung-Sik Korean Society of Environmental Health 2009 한국환경보건학회지 Vol.35 No.5
This study evaluated the bacterial concentrations and affecting factors at the laboratories of a university in Seoul, Korea. Thirty-three samples of total airborne bacteria (TAB) and eighteen samples of gram negative bacteria (GNB) were collected from both microbiology laboratories (7) and chemistry laboratories (6). GM (GSD) of TAB and GNB concentrations were 194 (2.52) $cfu/m^3$, 24 (4.1) $cfu/m^3$, respectively. TAB concentrations in the chemical laboratories (GM (GSD): 193 (2.0) $cfu/m^3$) were not significantly different from those in microbial laboratories (GM (GSD): 202 (2.7) $cfu/m^3$, (p>0.05)). GM (GSD) of TAB concentrationsat the top of sink, the center of laboratory, and the front of ventilation ventilation device within laboratories, 182 (3.2) $cfu/m^3$, 217 (2.2) $cfu/m^3$, 176 (2.4) $cfu/m^3$, respectively, were not significantly different (p=0.48). Related factors were measured such as temperature, relative humidity, floor of laboratory, number of persons and laboratory area. TAB concentrations were significantly related to temperature (r=0.36, p<0.05), and the floor of laboratory and temperature were also significantly related (r=0.49, p<0.001). However, other factors such as relative humidity, number of persons and laboratory area did not show any significant relationship with TAB concentrations (p>0.05). TAB concentrations were affected significantly by cleaning frequency (p<0.001) and floor of laboratory (p<0.05). There was also a significant difference (p<0.01) between TAB indoor concentrations and TAB outdoor concentrations. However, other factors such as general ventilation did not affect TAB concentrations (p>0.05) in this study.
Exposure Level of Airborne Bacteria in the University Laboratories in Seoul, Korea
Sung-Ho Hwang,Kyong-Nam Yoo,Ji-Ho Park,Dong-Uk Park,Chung-Sik Yoon 한국환경보건학회 2009 한국환경보건학회지 Vol.35 No.5
This study evaluated the bacterial concentrations and affecting factors at the laboratories of a university in Seoul, Korea. Thirty-three samples of total airborne bacteria (TAB) and eighteen samples of gram negative bacteria (GNB) were collected from both microbiology laboratories (7) and chemistry laboratories (6). GM (GSD) of TAB and GNB concentrations were 194 (2.52) cfu/m3, 24 (4.1) cfu/m3, respectively. TAB concentrations in the chemical laboratories (GM (GSD): 193 (2.0) cfu/m3) were not significantly different from those in microbial laboratories (GM (GSD): 202 (2.7) cfu/m3, (p>0.05)). GM (GSD) of TAB concentrationsat the top of sink, the center of laboratory, and the front of ventilation ventilation device within laboratories, 182 (3.2) cfu/m3, 217 (2.2) cfu/m3, 176 (2.4) cfu/ m3, respectively, were not significantly different (p=0.48). Related factors were measured such as temperature, relative humidity, floor of laboratory, number of persons and laboratory area. TAB concentrations were significantly related to temperature (r=0.36, p<0.05), and the floor of laboratory and temperature were also significantly related (r=0.49, p<0.001). However, other factors such as relative humidity, number of persons and laboratory area did not show any significant relationship with TAB concentrations (p>0.05). TAB concentrations were affected significantly by cleaning frequency (p<0.001) and floor of laboratory (p<0.05). There was also a significant difference (p<0.01) between TAB indoor concentrations and TAB outdoor concentrations. However, other factors such as general ventilation did not affect TAB concentrations (p>0.05) in this study.
Use of TachoSil to Prevent Pancreatic Leak following Distal Pancreatectomy
Doo-ho Lee,Jin-Young Jang,Youngmin Han,Joon Sung Park,Dong Sup Yoon,Jae Keun Kim,Ho-Seong Han,Yoo Seok Yoon,Dae Wook Hwang,Chang Moo Kang,Ho Kyong Hwang,Woo Jung Lee,Jin Seok Heo,Ye Rim Chang,Mee Joo 대한외과학회 2015 대한외과학회 학술대회 초록집 Vol.2015 No.05
Joung Sug Kim,Hyang-Mi Park,Kyong Mi Jun,Tae-Ho Lee,Duk-Ju Hwang,Sung-Dug Oh,Jong-Sug Park,Dae-Geun Song,Cheol-Ho Pan,Doil Choi,Yul-Ho Kim,Baek Hie Nahm,Yeon-Ki Kim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
The perturbation of the steady state of reactive oxygen species due to biotic and abiotic stresses in a plant could lead to protein denaturation through the modification of amino acid residues, including the oxidation of methionine residues. Methionine sulfoxide reductases (MSRs) catalyze the reduction of methionine sulfoxide back to the methionine residue. To assess the role of this enzyme, we generated transgenic rice using a pepper CaMSRB2 gene under the control of the rice Rab21 promoter with/without a selection marker, the bar gene. A drought resistance test on transgenic plants showed that CaMSRB2 confers drought tolerance to rice, as evidenced by less oxidative stress symptoms and a strengthened PSII quantum yield under stress conditions, and increased survival rate and chlorophyll index after the re-watering. The results from immunoblotting using a methionine sulfoxide antibody and nano-LC-MS/MS spectrometry suggest that porphobilinogen deaminase (PBGD), which is involved in chlorophyll synthesis, is a putative target of CaMSRB2. The oxidized methionine content of PBGD expressed in E. coli increased in the presence of H2O2, and the Met-95 and Met-227 residues of PBGD were reduced by CaMSRB2 in the presence of dithiothreitol. An expression profiling analysis of the overexpression lines also suggested that photosystems are less severely affected by drought stress. Our results indicate that CaMSRB2 might play an important functional role in chloroplasts for conferring drought stress tolerance in rice
Pregnane Glycoside Multidrug-Resistance Modulators from Cynanchum wilfordii
Hwang, Bang Yeon,Kim, Se Eun,Kim, Young Ho,Kim, Hang Sub,Hong, Young-Soo,Ro, Jai Seup,Lee, Kyong Soon,Lee, Jung Joon 충남대학교 약학대학 의약품개발연구소 1999 藥學論文集 Vol.15 No.-
The methanol-soluble extracts of the roots of Cynanchum wilfordii showed a significant multidrug-resistance-reversing activity, and four known pregnane glycosides were isolated by bioassay-directed fractionation and separation. Their structures were identified as gagaminin 3-0-β-D-cymaropyranoside (1), wilfoside KlN (2), wilfoside ClN (3), and cynauricuoside A (4). In particular, compound 1, at a concentration level of 1μM, was found to completely reverse the multidrug-resistance of KB-V1 and MCF7/ADR cells to adriamycin, vinblastine, and colchicine.
Acetophenones from the Roots of Cynanchum wilfordii HEMSLEY
Hwang, Bang-Yeon,Kim, Young-Ho,Ro, Jai-Seup,Lee, Kyong-Soon,Lee, Jung-Joon The Pharmaceutical Society of Korea 1999 Archives of Pharmacal Research Vol.22 No.1
Two acetophenones, cynandione A (1) and cynanchone A (2), were isolated from the roots of Cynanchum wilfordii. Their structures were identified by comparison of their physicochemical and spectral data with reported values.
자근으로부터 acetylshikonin의 분리 및 함량분석
황석연,황방연,강삼식,김창민,박정일,배기환,손건호,이승호,장승엽,강신정,노재섭,이경순 영남대학교 약품개발연구소 2001 영남대학교 약품개발연구소 연구업적집 Vol.11 No.-
Lithospermi Radix has been used in traditional medicine for antimicrobial, antiinflammatory, immunostimulating, antitumor and wound-healing activities. For the quality control of this drug, acetylshikonin was isolated from the hexane extract of Lithospermum erythrorhizon(Boraginaceae) and identified by the spectroscopic evidences. A quantitative analysis of acetylshikonin using HPLC method showed that the average contents were 0.084±0.026% in 31 samples collected throughout the various regions of Korea.