대두 식이섬유를 이용한 특수 영양식품의 유효성 평가

한재흠,이균희,손현수,이윤복,박점선,오만진 충남대학교 농업과학연구소 2008 농업과학연구 Vol.35 No.1

In the development of enteral foods for the patients with diarrhea, soybean hull, by-products of soybean processing, was used to prepare crude dietary fiber extracts (soybean hull fiber, SHF). Total dietary fiber content of SHF is 85% and their composition are 86.1% cellulose, 8.1% hemi-cellulose, and 4.7% lignin. The effects of SHF on the prevention of diarrhea were studied in animal. Spraque-Dawley (SD) rats were fed AIN93G diets containing 5% dietary fiber for 3days simultaneously inducing diarrhea with the phenolphthalein Mg citrate solution. On day 4, feces were collected at different time point. Dietary fibers used for the animal study were SHF, soybean cotyledon fiber (SCF), psyllium husk fiber (PHF), and chicory fiber (CF). α-cellulose was used as a control. Body weight gain, calorie consumed and food efficiency ratio among the experimental groups were not different. However, water content in the feces of SHF group was significantly lower by 10%, compared with other groups at 24hrs. time point. This effect was even greater in the feces collected later than 24 hrs. time point. SHF seems to have a greater effects on slow the symptom of diarrhea. Based on the previous results, enteral food enriched with SHF were prepared and its effect was compared with other commercially available products from domestic or imported ones. Weight changes among experimental groups were not different, but the moisture content of feces consumed SHF enriched products were lower than that of other products. Approximately 10% decrease in water content was observed from feces collected at 24 hr time point. According to the sensory evaluation, overall acceptability of the enteral food enriched with SHF was 3.24 out of 5 indicating that taste of this product is acceptable. 대두가공에 부산물로부터 얻어지는 대두 식이섬유를 이용하여 설사환자를 위한 경장 영양 식이를 개발하기 위하여 쥐에게 투여한 후 체중증가율, 변의 수분감소, 사료효율 등을 측정하여 다음과 같은 결과를 얻었다. 대두피 중의 식이섬유함량은 85% 이었고 cellulose 가장 높았으며 hemi-cellulose, lignin의 순 이었다. 대두 식이섬유가 대조구에 비하여 체중 감소율은 약간 낮았고 사료효율은 약간 높았다. 대두 식이섬유를 섭취시킨 쥐가 치커리 식이섬유, 차전자 피에 비하여 24시간 후의 변의 수분함량은 10% 낮았다. 대두 식이섬유를 첨가한 경장영양식이로 사육한 쥐의 체중변화와 사료효율은 3제품 간에 차이가 없었으나 다른 하나의제품은 대조군에 비하여 낮았다. 대두 식이섬유를 이용하여 가공한 경장영양식이 다른 상업용 제품에 비하여 실험쥐의 변 수분함량은 10% 이상 낮았다.

알코올성 췌장염 환자에서 CYP2E1 유전자형의 다형성

김일환,염윤기,채석,권준택,김형기,박상흠 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

Cytochrome P4502E1(CYP2E1) is inducible by ethyl alcohol and activates procarcinogenic N-nitrosodimethylamine(NDMA), benzene, urethane and other lower molecular weight compound. CYP2E1 is also involved in metabolism of certain drugs, for example, alcohol, acetaminophen, tamoxifen, theophylline, flouorinated anesthetics, and chlorozoxazone. CYP2E1 activity was shown to be polymorphically distributed in humans and has been suggested to play a role in hepatocellular carcinoma and alcoholic related disorders. Although genetic predispodition to alcoholism and alcoholic liver disease has been reported, genetic susceptibility to alcoholic pancreatitis is still a matter of debate. The aims of this study were to investigate the allelic frequency of CYP2E1 Rsa I polymorphism in Koreans with alcoholic pancreatitis patients. We investigated the frequency distribution of CYP2E1 Rsa I polymorphism in 212 unrelated healthy Koreans and 64 alcoholic pancreatitis patients. Detection of the CYP2E1 alleles was performed by polymerase chain reaction and restriction fragment length analysis. The genomic DNA was isolated from peripheral blood with conventional phenol: chloroforn extraction method. The allelic frequencies of c1 and c2 in healty volunteers were 0.85 and 0.15, respectively. The allelic frequencies of c1 and c2 in alcoholic pancreatitis patients were 0.8 and 0.2, respectively. The rare type (c2/c2) of CYP2E1 Rsa I polymorphism was 0.005 and 0.031 (p<0.001) in healthy volunteers and alcoholic pancreatitis patients, respectively. However, the CYP2E1 genotype showed no significant relation to the alcoholic pancreatitis patients. It was suggested that the risk of alcoholic pancreatitis diagnosed clinically seem to be not associated with the CYP2E1 genotype.

Paracyclopina nana Smirnov (Copepoda)의 생물학적 고찰 : Ⅰ. 각 유생 발생 단계별 탈피율 및 탈피에 소요되는 시간 Ⅰ. Moulting rate and inter-moult interval(days) of each larval developmental stages

김형신,정민민,박흠기,윤양호,이화자 濟州大學校 基礎科學硏究所 2002 基礎科學硏究 Vol.15 No.2

우리 나라 동해안에 위치한 화진포에서 채집된 요각류, Paracyclopina nana의 생물학적 특성을 알아보기 위하여 각 유생 단계의 탈피율 및 탈피에 소요되는 시간을 알아보았다. 수온 20℃, 염도농도 17ppt의 조건에서 T. suecica를 먹이로서 충분량 급이 했을 때 P. nana의 어미로부터 갓 부화되어 나온 nauplius 유생은 성체가 되기까지 평균 17일이 소요됨을 알 수 있었다. 특히 제 1 nauplius 유생 단계에서 제 2 nauplius 유생으로 탈피하는 데에 평균 3.3일이 소요되어 가장 많은 시간을 요하는 것으로 나타났다. 점차 탈피하는데 소요되는 기간은 짧아져서 제 5 nauplius 유생 단계에서 제 6 nauplius 유생 단계로 탈피하는데에 가장 짧아서 약 1일이 소요되었고, 제 2 copepodid 유생 단계에서 제 3 copepodid 유생 단계로 탈피하는데 약 2.5일이 소요되었다. 한편, 제 1 nauplius 유생 단계에서 제 2 nauplius 유생 단계로의 탈피율은 66.7% 그리고, 제 6 nauplius 유생단계까지의 탈피율은 50%를 나타내었다. The moulting rate and inter-moult interval(days) of each larval developmental stages of Paracyclopina nana were studied. The copepod, P. nana was collected from lagoon Hwajinpo, South Korea. The experimental conditions were temperature 20℃, salinity l7ppt and fed on micro-algae, Tetraselmis suecica. The species was metamorphosed to the adult stage after possession of 6 nauplius stages and 5 copepodid stages. The moulting rate was 66.7% from 1st nauplius stage to 2nd one and was 50.0% to 6th one. P. nana were needed 17 days for developing from hatching to adult.

The Role of Shamanism in Korean History

Yoon Yee-Heum(Yee-Heum Yoon) 전남대학교 종교문화연구소 2005 종교문화학보 Vol.1 No.-

Effects of gamma-irradiation before and after cooking on bacterial population and sensory quality of <i>Dakgalbi</i>

Yoon, Young Min,Park, Jong-Heum,Lee, Ji-Hye,Park, Jae-Nam,Park, Jin-Kyu,Sung, Nak-Yun,Song, Beom-Seok,Kim, Jae-Hun,Yoon, Yohan,Gao, Meixu,Yook, Hong-Sun,Lee, Ju-Woon Elsevier 2012 Radiation physics and chemistry Vol.81 No.8

<P><B>Abstract</B></P><P>The purpose of this study was to compare the effect of gamma irradiation on the total bacterial population and the sensory quality of <I>Dakgalbi</I> irradiated before and after cooking. Fresh chicken meat was cut into small pieces and used to prepare <I>Dakgalbi</I>. For the preparation of <I>Dakgalbi</I> cooked with gamma-irradiated chicken meat and sauce (IBC), raw chicken meat and <I>Dakgalbi</I> sauce were irradiated and then stir-fried. For the preparation of <I>Dakgalbi</I> irradiated after cooking with raw chicken meat and sauce (IAC), raw chicken meat and <I>Dakgalbi</I> sauce were first cooked and subsequently irradiated. Under the accelerated storage condition of 35°C for 7 days, bacteria in IBC were below the detection limit (1log CFU/g) on day 1 but were detected on day 2 and gradually increased hereafter. In IAC, on the other hand, bacteria were not detected at all. Evaluation of sensory quality also decreased on both samples. However, IAC showed a better trend. Our results indicate that IAC protocol was a more effective method for reducing bacterial growth in <I>Dakgalbi</I>.</P> <P><B>Highlights</B></P><P>► We compared the microbial safety and sensory property of <I>Dakgalbi</I> irradiated before and after cooking. ► <I>Dakgalbi</I> irradiated after cooking can be more effective processing method on microbial safety. ► Sensory property decreased on both <I>Dakgalbis</I> by irradiation-induced off-flavor. ► <I>Dakgalbi</I> irradiated after cooking showed a better tendency on the sensory evaluation.</P>

급성 척수손상 환자의 치료 : 최근의 세포치료 연구 결과를 중심으로

윤도흠,하윤,윤승환,김은영,현동근,박종운,박소라,구성욱,김긍년,조용은,박형천 대한척추신경외과학회 2004 Neurospine Vol.1 No.1

A therapy to treat injuries to the central nervous system is, to date, a major clinical challenge. The devastating functional consequences they cause in patients have encouraged many scientists to search, in animal models, for a repair strategy that could, in the future, be applied to humans. To highlight the current understanding of traumatic spinal cord injury, we provide information regarding state-of-the-art care for the acute spinal cord injured model, and explore future treatments aimed at neural preservation and reconstruction. This article also reviews recent phase I clinical trials in neural and nonneural cell transplantation, stressing that the transition from basic science to clinical applications.

Serotnin 1A Receptor Agonist(8 OH DPAT) Reduces Tissue Lesion Volume in the Rat Middle Cerebral Artery Occlusion Model of Ischemia

Yoon, Do Heum,Kim, Young Soo,Young, Wise 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.7

저자들은 쥐의 중대뇌동맥을 폐쇄시킨후 serotonin la agonist인 8 OH DPAT의 치료효과를 연구하였다. 중대뇌동맥 폐쇄 10분 후 부터 4시간 동안 30μng/㎏/hr의 8 OH DPAT을 정주하였으며 24시간 후에 대뇌 허혈부위에서 Na. K, H₂O의 함량을 측정하였다. 8 OH DPAT는 frontopyriform cortex와 frontoparietal cortex의 수분함량을 대조군에 비하여 각기 10%, 55% 감소시켰으며, Na 축척은 20%,47% 방지하였고. K손실은 24%, 44% 줄일 수 있었고 결국 lesion volume을 37% 줄일 수 있었다. 이 모든 변화는 모두 통계학적으로 의의가 있었다. 저자들의 이러한 결과는 8 OH DPAT가 쥐의 중대뇌동맥 폐쇄 후 발생하는 신경학적 손상을 현저히 줄일 수 있다는 것을 암시한다. We studied the effectiveness of serotonin la agonist(8 OH DPAT) on the 24 hour ionic lesion volume produced by permanent occlusion of the middle cerebral artery(MCAo) in rats. A 4-hour intravenous infusion of 30μmg/kg/hr of 8 OH DPAT were given starting at 10 minutes after occlusion. Tissue concentrations of Na. K. and water at infarct and peri-infarct zone were measured by atomic absorption spectroscopy and by wet-dry weight measurements 24 hours after occlusion. Compared with vehicle treatment. 8 OH DPAT treatment reduced tissue water accumulation by 10% and 55% in the frontopyriform cortex(L1) and frontoparietal cortex(L2). sodium accumulation by 20% at L1 and 47% at L2. potassium loss by 24% at Ll and 44% at L2. cell volume fraction loss by 24% at L1 and 47% at L2. Finally the treatment reduced overall lesion volume by about 37%. All these changes were statistically significant at p <0.05 Our findings suggest strongly that 8 OH DPAT is neuroprotective in the rat MCAo model of focal cerebral ischemia.

Han’guk Kodae Chonggyosasang (Ancient Religious thoughts of Korea)

Yoon Yee-heum(서평자) The Academy of Korean Studies 1986 Korea Journal Vol.26 No.7

The Diversity and Continuity of Shamanism in Korean Religious History

Yoon, Yee-heum 한국샤머니즘학회 2000 샤머니즘연구 Vol.3 No.-

Re-examination of the Empirical Category of Science of Religion

Yoon, Yee-heum 서울대학교 종교문제연구소 1995 종교와 문화 Vol.0 No.1